• Title/Summary/Keyword: Production Education

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High-frequency Plant Regeneration from Cultured Flower Bud Receptacles of Allium hookeri L.

  • Koo, Ja Choon
    • Horticultural Science & Technology
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    • v.32 no.5
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    • pp.694-701
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    • 2014
  • Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots ($93.33{\pm}4.63%$), roots ($76.67{\pm}7.85%$), and calli ($80.00{\pm}7.43%$) when cultured on Gamborg B5 (B5) medium containing $10{\mu}M$ 6-benzylaminopurine (BA) + $1{\mu}M$ naphthalene acetic acid (NAA), $0.5{\mu}M$ BA + $5{\mu}M$ NAA, and $1{\mu}M$ BA + $10{\mu}M$ NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to $10{\mu}M$. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.

Analysis of Usage Behavior for the Educational and Academic Information of Part-time Instructors (시간강사들의 교육학술정보 이용행태 분석)

  • Oh, Seon-Kyung
    • Journal of the Korean Society for Library and Information Science
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    • v.54 no.3
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    • pp.237-262
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    • 2020
  • Part-time instructors are contributing to the production of excellent human resources through education and research, strengthening research competitiveness, and national and social knowledge and academic development. Therefore, academic and research information services for part-time instructors are essential for their educational and research activities. In this study, researcher suggested various methods for providing efficient educational and academic information services by surveying and analyzing their information environment, information needs, and educational and academic information recognition and usage behavior by applying the survey method to part-time instructors.

A Study on the Fuzzy ELDC of Composite Power System Based on Probabilistic and Fuzzy Set Theories

  • Park, Jaeseok;Kim, Hongsik;Seungpil Moon;Junmin Cha;Park, Daeseok;Roy Billinton
    • KIEE International Transactions on Power Engineering
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    • v.2A no.3
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    • pp.95-101
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    • 2002
  • This paper illustrates a new fuzzy effective load model for probabilistic and fuzzy production cost simulation of the load point of the composite power system. A model for reliability evaluation of a transmission system using the fuzzy set theory is proposed for considering the flexibility or ambiguity of capacity limitation and overload of transmission lines, which are subjective matter characteristics. A conventional probabilistic approach was also used to model the uncertainties related to the objective matters for forced outage rates of generators and transmission lines in the new model. The methodology is formulated in order to consider the flexibility or ambiguity of load forecasting as well as capacity limitation and overload of transmission lines. It is expected that the Fuzzy CMELDC (CoMposite power system Effective Load Duration Curve) proposed in this study will provide some solutions to many problems based on nodal and decentralized operation and control of an electric power systems in a competitive environment in the future. The characteristics of this new model are illustrated by some case studies of a very simple test system.

Changes of PBP Quantity and FNR Activity by Light Wavelengths in Anabaena variabilis (光波長에 따른 Anabaena variabilis 의 Phycobiliprotein 含量 및 FNR 活性度 變化)

  • Kim, Jung-Suk;Chang, Nam-Kee
    • The Korean Journal of Ecology
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    • v.14 no.1
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    • pp.87-99
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    • 1991
  • Changes of phycobiliproteins(PBP) quantity and ferredoxin-NADP reductase(FNR) activity were investigated in various light illuminated cyanobacteria, Anabaena variabilis. PBP components were increased under blue light illumination, whereas decreased under red light illumination. PBP contents were twofolds in blue light than in red light. In view of the PBP composition, allophycocyanin(APC) in red light was higher 5.5% and phycoerythrocyanin(PEC) in blue light was higher 2.2% than in white light-illuminated PBP. It was suggested that PBP changes in bule light be the results of regulation of photosysthetic efficiency and protection of photosystem, whereas PBP changes in red light be effected by adaptation of adequate harvesting of light energy in photosystem. Changes of FNR activity were highest in red light, and sequenced lower to blue light and green light. It means that light-dependent production rate of NADP is the highest in red light. The difference of values was larger than that of values in comparison of red and blue light. It was suggested that increasing of FNR activity be due not to the function of isozyme, but to the synthesis of enzymes. Because of NAD/NADP regulation-effect to metabolism, it was considered that FNR activity might influence the metabolism indirectly and explain the probability of regulation in pathways of key enzyme activation. FNR activity was directly proportional to intensity of light. Optimum temperature and pH were about 25℃ and 7.5, respectively.

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Immunomodulating and Anticoagulant Activity of Glycosaminoglycans Derived from Porcine Testis

  • Yoo, Yung-Choon;Kim, Yeong-Shik;Song, Kyung-Sik;Moon, Eun-Ho;Lee, Kyung-Bok
    • Archives of Pharmacal Research
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    • v.25 no.5
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    • pp.669-674
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    • 2002
  • Glycosaminoglycans (GAGs) were isolated from the porcine testis, and their immuno-modulating and anticoagulant activity was investigated. From anion exchange chromatography (Dowex Macropolous Resin) used for further isolation of porcine testis GAGs (PT-GAGs), two fractions (PT-GAG-1.5 and PT-GAG-16) eluted by different salt concentration were obtained. In immunomodulating activity test, PT-GAG-1.5, but not PT-GAG-16, significantly enhanced the growth of murine peritoneal macrophages. In addition, treatment with PT-GAG-1.5 induced the production of cytokines, interleukin-1$\beta$ (IL-1$\beta$), interferon-${\gamma}$ (IFN-${\gamma}$) and tumor necrosis factor-$\alpha$ (TNF-$\alpha$), from murine microphages. Unexpectedly, both of PT-GAGs had no effect on the growth of murine splenocytes. The anticoagulant activity of PT-GAG-1.5 and PT-GAG-16 was examined by activated partial thromboplastin time (aPTT) assay and thrombin time (TT) assay. Both of PT-kGAGs significantly increased the clotting times of aPTT and TT in a dose-dependent manner. The anticoagulant activity of PT-GAG-16 was found to be higher than that of PT-GAG-1.5. These results suggest that PT-GAGs possess biological activities such as immunomodulating activity and anticoagulant activity.

Edible Sprout Production from Ainsliaea acerifolia Seeds

  • Park, Cheol-Ho;Lee, Cheol-Ki;Chang, Kwang-Jin
    • Plant Resources
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    • v.2 no.2
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    • pp.127-132
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    • 1999
  • This study was carried out to investigate the morphological characteristics of the plants and seeds of Ainsliaea acerifolia and to determine the optimum condition for producing sprouts from the seeds. Plant height, flower stalk length, and pod number were higher in natural habitat than in campus farm. Average 1.2 seeds per pod was set but only 0 to 2 seeds per plant was set in plants with the enveloped flower stalks, indicating that this is an outcrossing species. Most of seeds were 9-11mm long and 1.1-1.4mm wide. Fresh weight of seeds was ranged from 10mg to 17mg. Seeds germinated well at 15$^{\circ}C$ and 2$0^{\circ}C$. Mean germination period was 11.5 day at 15 to $25^{\circ}C$. Sprouts grown at 15$^{\circ}C$ was longest(5.4cm) and heaviest(738mg/10 sprouts). Chlorophyll content was 333mg per fresh weight 110g. Protein, Fe, vitamin Bl, vitamin B2, and vitamin C were respectively 23.7mg, 6.4mg, 1.82mg, 0.49mg, and 10..7mg.

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A Study on Industrial Development Direction at Transitional Periods of Industrial Structure in Chungcheongbuk-do Region (산업구조 전환기 충북지역 산업의 발전방향)

  • 한주성
    • Journal of the Economic Geographical Society of Korea
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    • v.6 no.2
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    • pp.293-306
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    • 2003
  • This paper aims to clarify the change in industrial structure and industry itself, and makes suggestions for the industrial development direction at transitional periods in the Chungcheongbuk-do(province) region. Because profits of regional gross production in Chungcheongbuk-do region flow out of the region, basic industries must be brought up. For this phenomenon, main manufacturing must be developed for the industrial power of the next generation of high added values that combined with digital industry; the petrochemistry, semiconductor industry as major type of industry, and automobile industry as minor type of industry. Also for supporting industry, education service, health and welfare, research and development services that are knowledge-based service industries in Chungcheongbuk-do region, must be formated the network among corporations and constructed regional innovation system linked with educational institutions, precision chemistry industry and biology technology as major type of industry, and precision machinery and tools industry as minor type of industry.

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A study on the usage of apparel CAD systems (국내 어패럴 CAD 시스템 사용현황에 관한 분석적 연구)

  • 최정욱;조진숙
    • Proceedings of the ESK Conference
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    • 1993.10a
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    • pp.165-188
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    • 1993
  • The study was to find out the current situation of supply and demand of apparel CAD systems in Korea. Also it was hoped to gather any valuable information about the curent usage of appared CAD systems for the development of the enhanced apparel CAD system. Three interviews were carried out for the study. The first interview with the importing agents was to find out the apparel CAD system's supplying situation. The second interview with the users of apparel CAD system was about the usage of the systems and related informations. The last interview was about the pattern making facilities, which was pointed out by the users to be the most inconvenient prat of apparel CAD systems. The results of the survey were:- 1. The supplu and demand of apparel CAD systems is increasing. 2. The education, training, maintenance of the system should be improved by the supplier. 3. Apparel CAD systems are used for various production systems in the apparel industry. 4. 100% of the systems are utilized for pattern input and 89% of the systems are utilized for marker making. Only 15% of the systems are utilized in pattern making process, which 80% of the users realize to be computerized. 5. The most serious barrier to the better usage of the system is the communication with the system in foreign language. This barrier could be lifted when Korean apparel CAD system is developed.

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Immunostimulating activity of maysin isolated from corn silk in murine RAW 264.7 macrophages

  • Lee, Jisun;Kim, Sun-Lim;Lee, Seul;Chung, Mi Ja;Park, Yong Il
    • BMB Reports
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    • v.47 no.7
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    • pp.382-387
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    • 2014
  • Corn silk (CS) has long been consumed as a traditional herb in Korea. Maysin is a major flavonoid of CS. The effects of maysin on macrophage activation were evaluated, using the murine macrophage RAW 264.7 cells. Maysin was isolated from CS by methanol extraction, and preparative $C_{18}$ reverse phase column chromatography. Maysin was nontoxic up to $100{\mu}g/ml$, and dose-dependently increased TNF-${\alpha}$ secretion and iNOS production by 11.2- and 4.2-fold, respectively, compared to untreated control. The activation and subsequent nuclear translocation of NF-${\kappa}B$ was substantially enhanced upon treatment with maysin ($1-100{\mu}g/ml$). Maysin also stimulated the phosphorylation of Akt and MAPKs (ERK, JNK). These results indicated that maysin activates macrophages to secrete TNF-${\alpha}$ and induce iNOS expression, via the activation of the Akt, NF-${\kappa}B$ and MAPKs signaling pathways. These results suggest for the first time that maysin can be a new immunomodulator, enhancing the early innate immunity.

Section Analysis of EMS Rail by Finite Element Analysis (유한요소해석을 통한 EMS 레일 단면 해석)

  • Yu, Byoung Kwon
    • Transactions of the KSME C: Technology and Education
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    • v.3 no.1
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    • pp.63-69
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    • 2015
  • Among the material handling system, EMS (Electric Monorail System), which is the facility of transferring the material hanging on truss, has the strength point of the maximum utilization of working space and the improvement of working environment including low-level noise generation. This paper will introduce the variable method of EMS rail analysis, which has the main role of supporting the whole material weight and guiding them with high-speed transportation, and, based on the analysis, the direction of optimization of the rail design be described. The rail with light-weight and high-strength contributes the reduction of the load of truss, the cost-down of rail production and the easy-installation on site.