• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.92-100
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• 2017

Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3-BD) was obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from Serratia sp. T241, Lactobacillus brevis, and Vitreoscilla, respectively. The new biocatalyst of E. coli/pET-mbdh-nox-vgb was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3R)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l meso-2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3R)-AC production. The results exhibit the industrial potential for (3R)-AC production via whole-cell biocatalysis.

• 공학교육연구
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• 제15권5호
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• pp.64-68
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• 2012

Design factors such as design objects establishment, analysis, synthesis, production, test and evaluation should be educated in a systematic way. Also design ability to reflect practical restrictive conditions such as industrial standards, economic feasibility, environmental impact, aesthetics, safety and reliability, ethical impacts and social impacts should be cultivated. In this paper, we explain the meaning of these terms and propose a systematic engineering design education framework satisfying Korean engineering education accreditation criteria. We also present a simple implementation in information network engineering.

• 환경정책연구
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• 제8권4호
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• pp.51-73
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• 2009

오늘날 환경교육의 중요성이 대두되고 있음에도 불구하고 환경교육이 에너지 절감 또는 쓰레기 절감에 미치는 정량적 효과에 대한 연구는 거의 전무한 실정이다. 본 연구는 환경교육이 집중적으로 행해지는 집단과 일반적으로 행해지는 집단의 에너지 및 쓰레기 절감 효과를 정량적으로 분석한다. 본 연구의 분석결과, 환경교육의 강화는 에너지 절감 및 쓰레기 절감에 부분적으로 긍정적인 영향을 미치는 것으로 나타났다. 구체적으로 환경교육이 집중적으로 이루어진 학생들 가정에서의 제 요금 및 학교에서 남기는 음식의 양이 일반적인 환경교육이 이루어지는 학생들 가정에서의 제 요금 및 학교에서 남기는 음식의 양보다 유의하게 낮은 것으로 나타난다. 본 연구의 결과는 에너지 절감 및 쓰레기 절감을 위해 환경시범학교 등과 같은 다양한 환경교육 정책의 강화가 필요하다는 것을 보여준다고 할 수 있다.

• 디지털융복합연구
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• 제17권7호
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• pp.341-349
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• 2019

오늘날 미술교육은 확장된 장에서 매체 자체나 매체를 통한 이미지 제작 교육이 필요하다. 본 연구는 학생들이 기초적 영상 문법을 습득하여 영상의 기본 구성요소들을 이해하고 동시에 소통 능력 중 발문과 경청 능력, 그리고 이해와 정리 능력을 향상시키기 위해 기초 영상 문법 습득과 소통 능력 향상을 위한 융합 수업으로 인터뷰 영상 제작 수업지도안을 개발하고 적용하였다. 수업은 중학생 30명을 대상으로 3차시로 진행되었으며 사전, 사후 질문 및 학생 작품분석을 통해 얻은 결과는 다음과 같다. 첫째, 학생들은 인터뷰 영상 제작 수업을 통해 촬영의 샷 사이즈와 안정된 구도를 사용했으며, 결과적으로 기초 영상 문법 습득에 본 수업이 효과적이었다. 둘째, 학생들은 인터뷰 영상 제작 수업을 통해 발문, 경청, 이해, 그리고 정리 능력이 향상되었다. 결과적으로 학생들은 인터뷰 영상 제작 수업을 통해 기초 영상문법을 적용한 인터뷰 영상을 효과적으로 제작하였다.

• 수산해양교육연구
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• 제26권2호
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• pp.444-454
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• 2014

The range of optimization problem in aquaculture is very wide, resulting from the range of species, mode of operation. Quite a few studies focus marine net-cages, but studies on land based culture farm are few or no. This paper considers a allocation problem to meet production planning in land based aquaculture system. A water pool allocation model in land based aquaculture system was developed. The solution finds the value of decision variable to minimize yearly production costs that sums up the water pool usage cost and sorting cost. The model inputs were (1) the fish growth rate (2) critical standing corp (3) number of water pool (4) number of fish. The model outputs were (5) number of water pool in growing phase (6) cost of cultivation (6) optimal facility allocation(number of water pool for each growing phase). To solve the problem, an efficient heuristic algorithm based on a greedy manner is developed. Branch and bound and heuristic is evaluated through numerical examples.

• 수산해양교육연구
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• 제25권2호
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• pp.410-418
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• 2013

Based on the survey on abalone aquaculture management status in Nohwa-eup, Bogil-myeon, and Wando-eup in Wando region, this study compared the analyzed business performance generated from production by region. The results show that the abalone production quantity has been stabilized currently after rapid growth during the past 10 years. In the future, it will be the time for more positively spontaneous efforts by people in aquaculture business to restore fishing ground, in which they have actively and continuously worked, by means of clean-up of fishing ground, movement of fishing ground, or time-off period rather than just to focus on the short-term increase of production in all of three regions.

• Asian-Australasian Journal of Animal Sciences
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• 제16권3호
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• pp.455-462
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• 2003

Biotechnological research and development are moving at a very fast rate. The subject has assumed greatest importance in recent years in the development of agriculture and human health. The science of biotechnology has endowed us with new tools and tremendous power to create novel genes and genotypes of plants, animals and fish. The application of biotechnology in the fisheries sector is a relatively recent practice. Nevertheless, it is a promising area to enhance fish production. The increased application of biotechnological tools can certainly revolutionise our fish farming besides its role in biodiversity conservation. The paper briefly reports the current progress and thrust areas in the use of synthetic hormones in fish breeding, production of monosex, uniparental and polyploid individuals, molecular biology and transgenesis, biotechnology in aquaculture nutrition and health management, gene banking and the marine natural products.

• 수산해양교육연구
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• 제28권5호
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• pp.1280-1289
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• 2016

This study aims to conduct economic analysis of the Rainbow Trout (Onchorhynchus mykiss) aquaculture farms in Korea. The analysis was performed based on farming cost, market price of the species and fishermen's income. We estimated the farms' economic feasibility using return on sales, the NPV, the IRR and the break-even point. The result indicated that while the profitability depends on current aquaculture production performance and market situation, the business operation is up to price and aquaculture production of species. According to sensitivity analyses of price and yield, aquaculture business becomes poorer with lower price and production.

• 반도체디스플레이기술학회지
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• 제15권3호
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• pp.72-77
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• 2016

As the semiconductor production technology has gradually developed and intra-market competition has grown fiercer, the caliber of Si Wafer for semiconductor production has increased as well. And semiconductors have become integrated with higher density. Presently the Si Wafer caliber has reached up to 450 mm and relevant production technology has been advanced together. Electrostatic chuck is an important device utilized not only for the Wafer transport and fixation but also for the heat treatment process based on plasma. To effectively control the high calories generated by plasma, it employs a refrigerant-based cooling method. Amid the enlarging Si Wafers and semiconductor device integration, effective temperature control is essential. Therefore, uniformed temperature distribution in the electrostatic chuck is a key factor determining its performance. In this study, the form of refrigerant flow channel will be investigated for uniformed temperature distribution in electrostatic chuck.

• 수산해양교육연구
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• 제19권1호
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• pp.150-160
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• 2007

The school system of fisheries high school was proper to specialist objective school system in order to training for expert human resource development.Training a field of human resources development from fisheries high school is fallow; fisheries production, seamen's training, ship engine and refrigerator, marine electronic telecommunication and information, fisheries foods production and fisheries foods production and distribution, fishery fisheries self-management, marine distribution, management and conservation of marine environment, safety and marine prevention of disasters, apparatus of marine development, under water area development.A new department opening and each department was revised toward to department name and department character. The unit-lesson hour of curriculum according to specialist objective school system of fisheries and marine highschool was revised. professional subject 98 unit-lesson hour(52%), normality subject 90 unit-lesson hour(48%), and educational activity of professional subject 10unit-lesson hour, total training activity 10 unit-lesson hour. And the special objective school system need to revise curriculum of 208 total unit-lesson hour.