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The Impact of Characteristics of Communication Media and Instruction Behavior on Collaborative Interaction and Project Performance (커뮤니케이션매체 특성과 교수행위 특성이 협력적 상호작용과 프로젝트 성과에 미치는 영향)

  • Ko, Yun-Jung;Chung, Kyung-Soo;Ko, Il-Sang
    • Asia pacific journal of information systems
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    • v.18 no.4
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    • pp.83-103
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    • 2008
  • In the new web based learning environment which has recently emerged, a variety of new learning objectives and teaching methods suited to this learning environment have been adopted. Recently, web based project-based learning methods have received a great deal of attention from those wishing to improve learning performance. The objective of this study is to identify the impact of characteristics of communication media and instruction behavior on collaborative interaction and project performance through web based group projects. The characteristics of communication media were divided into richness, flexibility, and ease of use, and the characteristics of instruction behavior were divided into support and expression, which are independent variables. Collaborative interaction as a mediate variable, was divided into information sharing and negotiation. Project performance was the dependent variable. To verify the proposed research model empirically, an experiment was conducted in which learners participated in on-line and off-line courses with group projects. The group project was conducted virtual product development(VPD), and designed a web-site about the VPD. At the end of the project, a survey was conducted. Of the 270 students, 239 responded. The students were assigned to groups of 3 or 4 members, and represented different genders and levels of computer competence. The reliability, validity, and correlation of research variables were analyzed using SPSS 14.0, and the measurement model and the structural goodness-of-fit of the research model were verified through SEM analysis using Lisrel 8.54. We found important results as follows; First, richness and ease of use has positive impacts on each of sharing information and negotiation. This suggests that richness and ease of use are useful in sharing information which is related to the task and agreeing in opinions among group members. However, flexibility has not positive impacts on sharing information and negotiation. This implies that there is no great difference in performance of PC and information literacy of user. Second, support and expression of instructor have positive impacts on sharing information and negotiation. This indicates that instructors play an important role in encouraging learners to participate in the project and communicating with them, sharing information related to the project, making a resonable decision and finally leading them to improve a project performance. Third, collaborative interaction has a positive impact on project performance. This result shows that if the ability to share information and negotiate among students was improved then a project performance would be improved as well. Recently, in the state of revitalized web based learning, it is opportune that web-based group project is practically conducted, and the impact of characteristics of communication media and characteristics of instruction behavior on sharing information, negotiating among group members and improving a project performance is verified. On the basis of these results, we propose that forms of learning, such as web based project, could be one of solution which is to enforce interaction among learners, and ultimately improve learning performance. Moreover web-based group project is able to make up for a weakness which makes it difficult to make interpersonal relations or friendship among learners in computer mediated communication or web based learning.

Isolation of Anticarcinogenic Isoflavone-conjugated Glycoproteins from a Submerged Liquid Culture of Agaricus blazei Mycelia by the Autolysis Process (신령버섯균사체 액체배양물의 자가분해에 의한 항암성 isoflavone-conjugated glycoprotein 분리)

  • Kim, So Young;Kim, Young Suk;Jang, Joung Soon;Kim, Boh Hyun;Rakib, Abdur Md.;Kim, Gon Sup;Kim, Jeong Ok;Ha, Yeong Lae
    • Journal of Life Science
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    • v.24 no.12
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    • pp.1316-1324
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    • 2014
  • Most beta-glucans obtained from various fruit bodies of mushrooms and mushroom mycelial cultures have high-molecular weight glycoproteins, conjugated with beta-glucans. We report that isoflavone-conjugated glycolproteins (designated as gluvone) were isolated and exhibited stronger anticarcinogenic activities. Agaricus blazei mycelia (ABM) was cultured in a liquid medium containing soybean flakes for 14 days. The liquid culture was autolyzed by incubating at $53^{\circ}C$ (pH 5.5) for 3 h. A crude glycoprotein (CGP) fraction with a cytotoxic effect on a mouse ascite cancer cell line (S-180) and a human breast cancer cell line (MCF-7) was isolated from the autolyzed ABM cultures by 80% ethanol treatment. Gluvone was isolated from the CGP with Sephadex G-75 column chromatography. It exhibited a stronger anticancer effect than CGP against the S-180 cell-induced female ICR mouse ascites carcinogenesis. Gluvone with 9,400 daltons was identified as a glycoprotein conjugated with isoflavone. According to HPLC and GC analysis, in conjunction with $^1H$-NMR spectral analysis, it contained 60% carbohydrates (glucose, fructose, and ribose), 31% protein, and 2% isoflavone (daidzein and genistein), which is a novel material. These results indicate that a strong anticarcinogenic gluvone was isolated from the autolyzed product of a submerged liquid culture of ABM, suggesting that autolysis could be a useful tool to produce antitumor agents.

A Study on the Manufacturing Process of Ladies' Jacket (숙녀복(淑女服) 재킷 제조공정(製造工程) 실태(實態) 연구(硏究))

  • Shim, Jae-Hee;Sohn, Hee-Soon
    • Journal of Fashion Business
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    • v.6 no.1
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    • pp.43-52
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    • 2002
  • The purpose of this study was to present the plan for activation and rationalization of production of ladies' jacket and provide basic materials for improvement of the development of technologies in relation to the productivity improvement of ladies' jacket and the achievement of high quality product. For this purpose, this study attempted to investigate the present situation of jacket manufacturing process. The data is related with local ladies' jacket manufacturing companies in Seoul snd Kyonggi area. The results of this study are as follows. 1. 87.8% of the business firms responded that they acquired the productive process of jacket based on their own knowhow and 80.5% was aware of the need for the analysis of each process. The highest proportion of the business firms(65.9%) pointed out that the advantage of process analysis was the alleviation of the production time. 2. The jacket manufacturing process was made up of 4 stages such as the process of frontal/rear plate $\rightarrow$ the process of accessories $\rightarrow$ the process of completion $\rightarrow$ the process of finishing in a broad sense but composed of a total of 19 stages in detail. 3. Attachment of the sleeves(73.2), attachment of the collar(41.5%) and the formation of the overall silhouette(22.0%) were raised as the challenge in manufacturing ladies' jacket. 4. Most of the sewing business firms made use of the method of completing the collar and then stitching the outer material and the inner collar, and the line of the bodice and the outer collar as the method of stitching the tailored collar. and many of them used the method of completing the collar and then inserting it between the line of the bodice and the outer material and stitching it as the method of stitching the stand collar. They had a preference for the method of completing the sleeve and connecting it to the bodice as the method of stitching the sleeve. and used the method of treating the margin to seam of semi-lined and unlined jacket by treating it with the bias tape.

Protein Kinase C (PKC) in Cellular Signalling System: Translocation of Six Protein Kinase C Isozymes in Human Prostate Adenocarcinoma PC-3 Cell Line (세포신호계에 있어서 Protein Kinase C: 사람의 전입선 adenocarcinoma PC-3 세포내의 여섯개의 Protein kinase C 동립효소의 translocation)

  • Park, Won-Chul;Ahn, Chang-Ho
    • The Korean Journal of Zoology
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    • v.36 no.4
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    • pp.439-451
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    • 1993
  • Protein kinase C isozymes in a human prostate adenocarcinoma PC-3 cell line were characterized. Immunoreactive bands and immunocytochemical stains were obsenred in PC-3 cells with antibodies raised against protein kinase C ${\alpha}$, ${\beta}$, ${\gamma}$, $\delta$, $\varepsilon$, and ζ types, respectively. Protein kinase C ${\alpha}$ corresponded to a immunoreactive band at a molecular weight of 80,000-dalton, whereas molecular weights of other immunoreactive isozvmes of protein kinase C were detected at 68,000-dalton. Protein kinHse C $\delta$ and ζ antibodies detected additional bands at 55,000-dalton and 80,000-dalton, respectively Immunocvtochemical study confirmed the results of the immunoblotting experiments qualitatively: all six protein kinase C isozymes were detected in the cytoplasm of PC-3 cells. Translocation of protein kinase C in PC-3 cells were also examined with phorbol 12-myristate 13-acetate (PMA), bryostatin 2, diolein, and 1-oleoyl-2-acetyl glycerol (OAG). Differential reactions of protein kinase C isozvmes to these activators were obsenred. When PC-3 cells were treated with 10mM bryostatin 2, protein kinase C isozyme u was translocated into the nucleus, whereas s type was translocated into the plasma membrane and the nucleus. Protein kinase C ${\alpha}$ and ζ types were translocated into the nucleus following the treatment with 101M diolein, whereas protein kinase C ${\alpha}$, ${\beta}$, ${\gamma}$, and $\varepsilon$ types were translocated into the nucleus by the treatment with 10mM OAG. Protein kinase C ${\alpha}$ and $\varepsilon$ types were translocated into the nucleus in the presence of 100nM PMA. Protein kinase C $\delta$ type was translocated to the nuclear membrane by these activators, however, only PMA-induced translocation was inhibited by protein kinase C inhibitor, 1-(5-isoquinolinesulfonyll-2-methvlpiperazine dihvdrochloride (H7) . H7 inhibited translocation of protein kinase C ${\alpha}$ type induced by PMA, ${\beta}$ type by OAG and s type by PMA and OAG, whereas it did not affect translocations induced by bryostatin and diolein, respectively. These results suggest that there exist six isoformes of protein kinase C (${\alpha}$, ${\beta}$, ${\gamma}$, $\delta$, $\varepsilon$ and ζ types) in PC-3 cells and that each of these isozvmes distinctivelv reacts to bryostatin, diolein, OAG and PMA, in part due to an altered molecular size and conceivably discrete binding site(s).

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The Effect of Linarin on LPS-Induced Cytokine Production and Nitric Oxide Inhibition in Murine Macrophages Cell Line RAW264.7

  • Kim, Kyung-Jae;Han, Shin-Ha;Sung, Ki-Hyun;Yim, Dong-Sool;Lee, Sook-Kyeon;Lee, Chong-Kil;Ha, Nam-Ju
    • Archives of Pharmacal Research
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    • v.25 no.2
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    • pp.170-177
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    • 2002
  • The herb, Chrysanthemum zawadskii var, latilobum commonly known as Gu-Jul-Cho in Korea, used in traditional medicine to treat pneumonia, bronchitis, cough, common cold, pharyngitis, bladder-related disorders, gastroenteric disorders, and hypertension. Linarin is the main active compound and the biological mechanisms of its activity are unclear. It is believed that effects of this herb may be exerted through the pluripotent effectors of linarin due to its ability to treat a variety of afflictions. In this study, the effects of linarin on the mouse macrophages cell line, RAW 264.7, were investigated. It was found that linarin could activate macrophages by producing cytokines. Monocytes and tissue macrophages produce at least two groups of protein mediators of inflammation, interleukin 1 (IL-1 ) and the tumor necrosis factor (TNF). Recent studies have shown that TNF and IL-1 modulate the inflammatory function of endothelial cells, leukocytes, and fibroblasts. $TNF-{\alpha}$ production by macrophages treated with linarin occured in a dose dependent manner However, IL-1 production was largely unaffected by this natural product. This study demonstrated the ability of linarin to activate macrophages both directly and indirectly. Linarin also affect both cytosine production and nitric oxide inhibition, in addition to the expression of some surface molecules. Nitric oxide (NO), derived from L-argin-ine, is produced by two forms(constitutive and inducible) of nitric oxide synthase (NOS). The NO produced in large amounts by inducible NOS is known to be responsible for the vasodilation and hypotension observed in septic shock. Linarin was found to inhibit NO production in the LPS-activated RAW 264.7 cells. Linarin may be a useful candidate as a new drug for treating endotoxemia and the inflammation accompanied by NO overproduction. The linarin-treated total Iymphocytes exhibited cytotoxicity in a dose dependent manner between $20{\;}{\mu}g/ml{\;}and{\;}40{\;}{\mu}g/ml$. These results suggest that linarin may function through macrophage activation.

A Study on the Cognitive Characteristics of Analog Image and Digital Image as a Symbolic Function in the Two-Dimensional Smartphone Form (2차원 스마트폰형상에 있어서 상징적 기능으로서 아날로그이미지 및 디지털이미지의 인지특성 연구)

  • Lee, Jin Seung;Kim, Jin Sung
    • Journal of the HCI Society of Korea
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    • v.13 no.3
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    • pp.17-33
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    • 2018
  • Humans process aesthetic functions of objects such as form, size, color, and texture through vision, while at the same time observe them with a symbolic function by a new psychological interpretation based on their past experience. Therefore, it is more important to study and understand the thoughts and psychological influences of how humans perceive with their eyes than what humans simply look with them. With this research purpose in the background, this study will demonstrate in detail the cognitive characteristics of analog and digital images as a symbolic function with a "formative" point of view. Five hypotheses were established, which were proven through the following tests: smartphone, most frequently used in our daily lives, is simplified to a two-dimensional line drawing form, which is set to instances where the R(radius) is applied to the horizontal line of the two-dimensional form of smartphone, where Run-in-R(radius) is applied, and R(radius) rounding is applied to the edges of those shapes. The shapes were combined to form 12 pairs, which was used in a test to the test subjects. As a result, some human characteristics were observed; in specific, some helped prove objectively how humans perceived analog and digital images as psychologically stronger as a symbolic function. If people's thoughts and feelings gained through such study could be stored in database as one value and be provided to product designers, I believe it could be developed into design form as a basic emotional design that consumers desire.

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A Study of Electromagnetic Coupling Analysis between Dipole Antenna and Transmission Line Using PEEC Method (PEEC 방법을 이용한 다이폴 안테나와 전송선로 사이의 전자기 결합 분석에 관한 연구)

  • Oh, Jeongjoon;Kim, Kwangho;Park, Myeongkoo;Lee, Hosang;Nah, Wansoo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.28 no.11
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    • pp.902-915
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    • 2017
  • In recent years, mobile devices have become increasingly multi-functional and high performance, resulting in a dramatical increase in processing speed. On the other hand, the size of device is reduced, circuits inside the device are more easily exposed to electromagnetic interference radiated from antenna or adjacent circuits, degrading the system performance. To prevent this, it is necessary to design the device considering the electromagnetic characteristics with EM simulation at the design stage of product. However, the EM simulation takes a long analysis time and require high-level system resources for fast analysis. In this paper, an equivalent circuit modeling method for a round wire is proposed using a PEEC method and the electromagnetic coupling from a dipole antenna to a transmission line is analyzed in frequency domain. And compared with the result of electromagnetic simulator. As a result, PEEC method shows good agreement with those of electromagnetic simulation, in a much more short time.

A Fundamental Study on the Development of the Fusion Education Curriculum in the Field of Architecture and Urban: based on Practitioner-oriented Survey (건축·도시 분야 융복합 교육과정 개발을 위한 기초연구 -실무자 중심의 설문조사를 기반으로-)

  • Hong, So-Hee;Yoo, Seung-Kyu;Yuh, Ok-Kyung;Kim, Jae-Jun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.5
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    • pp.3491-3498
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    • 2015
  • The purpose of this study is to identify demand of fusion education in the field of architecture and urban, according to architecture and urban market changing, product changing, increasing fusion education demand. We conducted online surveys targeting construction and non construction field practitioners so that we want to identify consumer's needs about fusion education in the field of architecture and urban. Research findings can be summarized in three parts. First, practitioners need to fuse business-related majors (Frequency changing ratio : financing(716.7%), management(633.3%), planning(454.5%) etc.) and traditional majors. Second, the survey results show that 79 percent of those questioned were in favor of switching the cyber and off-line operation methods for fusion education. They require the complementary education system between off-line and cyber education based on education accessability. Third, the survey results show that 70.9 percent of those questioned were in favor of limited freestyle(Freedom of choices without required subjects). The curriculum should be arranged according to the flow of construction business process including fusion subjects.

Ansanella granifera gen. et sp. nov. (Dinophyceae), a new dinoflagellate from the coastal waters of Korea

  • Jeong, Hae Jin;Jang, Se Hyeon;Moestrup, Ojvind;Kang, Nam Seon;Lee, Sung Yeon;Potvin, Eric;Noh, Jae Hoon
    • ALGAE
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    • v.29 no.2
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    • pp.75-99
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    • 2014
  • A small dinoflagellate, Ansanella granifera gen. et sp. nov., was isolated from estuarine and marine waters, and examined by light microscopy, scanning electron microscopy, and transmission electron microscopy. In addition, the identity of the sequences (3,663-bp product) of the small subunit (SSU), internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), and D1-D3 large subunit (LSU) rDNA were determined. This newly isolated, thin-walled dinoflagellate has a type E eyespot and a single elongated apical vesicle, and it is closely related to species belonging to the family Suessiaceae. A. granifera has 10-14 horizontal rows of amphiesmal vesicles, comparable to Biecheleria spp. and Biecheleriopsis adriatica, but greater in number than in other species of the family Suessiaceae. Unlike Biecheleria spp. and B. adriatica, A. granifera has grana-like thylakoids. Further, A. granifera lacks a nuclear fibrous connective, which is present in B. adriatica. B. adriatica and A. granifera also show a morphological difference in the shape of the margin of the cingulum. In A. granifera, the cingular margin formed a zigzag line, and in B. adriatica a straight line, especially on the dorsal side of the cell. The episome is conical with a round apex, whereas the hyposome is trapezoidal. Cells growing photosynthetically are $10.0-15.0{\mu}m$ long and $8.5-12.4{\mu}m$ wide. The cingulum is descending, the two ends displaced about its own width. Cells of A. granifera contain 5-8 peripheral chloroplasts, stalked pyrenoids, and a pusule system, but lack nuclear envelope chambers, a nuclear fibrous connective, lamellar body, rhizocysts, and a peduncle. The main accessory pigment is peridinin. The SSU, ITS regions, and D1-D3 LSU rDNA sequences differ by 1.2-7.4%, >8.8%, and >2.5%, respectively, from those of the other known genera in the order Suessiales. Moreover, the SSU rDNA sequence differed by 1-2% from that of the three most closely related species, Polarella glacialis, Pelagodinium bei, and Protodinium simplex. In addition, the ITS1-5.8S-ITS2 rDNA sequence differed by 16-19% from that of the three most closely related species, Gymnodinium corii, Pr. simplex, and Pel. bei, and the LSU rDNA sequence differed by 3-4% from that of the three most closely related species, Protodinium sp. CCMP419, B. adriatica, and Gymnodinium sp. CCMP425. A. granifera had a 51-base pair fragment in domain D2 of the large subunit of ribosomal DNA, which is absent in the genus Biecheleria. In the phylogenetic tree based on the SSU and LSU sequences, A. granifera is located in the large clade of the family Suessiaceae, but it forms an independent clade.

Sun-induced Fluorescence Data: Case of the Rice Paddy Field in Naju (논벼에서 관측된 태양 유도 엽록소 형광 자료: 나주에서 2020년 6월 10일부터 10월 5일까지)

  • Ryu, Jae-Hyun;Jang, Seon Woong;Kim, Hyunki;Moon, Hyun-Dong;Sin, Seo-Ho;Lee, Yang-Won;Cho, Jaeil
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.23 no.1
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    • pp.82-88
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    • 2021
  • Sun-induced fluorescence (SIF) retrieval using remote sensing technique has been used in an effort to understand the photosynthetic efficiency and stress condition of vegetation. Although optical devices and SIF retrieval methodologies were established in order to retrieve SIF, the SIF measurements are domestically sparse. SIF data of paddy rice w as measured in Naju, South Korea from June 10, 2020 to October 5, 2020. The SIFs based red (O2A) and far-red (O2B) w ere retrieved using a spectral fitting method and an improved Fraunhofer line depth, and photosynthetically active radiation was also produced. In addition, the SIF data was filtered considering solar zenith angle, saturation conditions, the rapid and sudden change of solar irradiance, and sun glint. The provided SIF data can help to understand a SIF product and the filtering method of SIF data can contribute to producing high-quality SIF data.