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http://dx.doi.org/10.5352/JLS.2014.24.12.1316

Isolation of Anticarcinogenic Isoflavone-conjugated Glycoproteins from a Submerged Liquid Culture of Agaricus blazei Mycelia by the Autolysis Process  

Kim, So Young (Department of Food and Nutrition, International University of Korea)
Kim, Young Suk (HK Biotech, Co. Ltd.)
Jang, Joung Soon (Department of Internal Medicine, Chung-Ang University College of Medicine)
Kim, Boh Hyun (Department of Applied Chemistry, Institute of Agriculture and Life Science, Gyeongsang National University)
Rakib, Abdur Md. (Department of Applied Chemistry, Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Gon Sup (Laboratory of Biochemistry, School of Veterinary Medicine, Gyeongsang National University)
Kim, Jeong Ok (HK Biotech, Co. Ltd.)
Ha, Yeong Lae (Department of Applied Chemistry, Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Journal of Life Science / v.24, no.12, 2014 , pp. 1316-1324 More about this Journal
Abstract
Most beta-glucans obtained from various fruit bodies of mushrooms and mushroom mycelial cultures have high-molecular weight glycoproteins, conjugated with beta-glucans. We report that isoflavone-conjugated glycolproteins (designated as gluvone) were isolated and exhibited stronger anticarcinogenic activities. Agaricus blazei mycelia (ABM) was cultured in a liquid medium containing soybean flakes for 14 days. The liquid culture was autolyzed by incubating at $53^{\circ}C$ (pH 5.5) for 3 h. A crude glycoprotein (CGP) fraction with a cytotoxic effect on a mouse ascite cancer cell line (S-180) and a human breast cancer cell line (MCF-7) was isolated from the autolyzed ABM cultures by 80% ethanol treatment. Gluvone was isolated from the CGP with Sephadex G-75 column chromatography. It exhibited a stronger anticancer effect than CGP against the S-180 cell-induced female ICR mouse ascites carcinogenesis. Gluvone with 9,400 daltons was identified as a glycoprotein conjugated with isoflavone. According to HPLC and GC analysis, in conjunction with $^1H$-NMR spectral analysis, it contained 60% carbohydrates (glucose, fructose, and ribose), 31% protein, and 2% isoflavone (daidzein and genistein), which is a novel material. These results indicate that a strong anticarcinogenic gluvone was isolated from the autolyzed product of a submerged liquid culture of ABM, suggesting that autolysis could be a useful tool to produce antitumor agents.
Keywords
Agaricus blazei mycelia (ABM); autolysis; MCF-7 cancer cells; S-180 cancer cells; submerged-liquid culture;
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