• Journal of Korean Society for Atmospheric Environment
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• v.21 no.6
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• pp.639-647
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• 2005

A single particle analytical technique, named low-Z particle electron probe X-ray microanalysis (EPMA), was applied to characterize samples collected at a subway station and ambient samples in Seoul. According to their chemical composition, many distinctive particle types were identified. For samples collected at the subway station platform, the major chemical species are carbon-rich, organic, aluminosilicates (AlSi), AlSi/C, AlSi/$CaCO_{3},\;CaCO_{3},\;SiO_{2},\;and\;Fe_{2}O_{3}$. For outdoor samples, carbon-rich, organic, AlSi, $CaCO_{3},\;SiO_{2},\;NaNO_{3},\;(Na,Mg)NO_{3},\;Na(CO_{3},NO_{3},SO_{4}),\;and\;(NH_{4})_2SO_4$, are abundantly encountered. Samples collected at the subway station show very high contents of $Fe_{2}O_{3}$, both in coarse and fine fractions, which come from brake block, subway train wheel, electric contact materials, etc. It is demonstrated that the single-particle characterization using this low-Z particle EPMA technique provided detailed information on various types of chemical species in indoor and outdoor samples.

• Microbiology and Biotechnology Letters
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• v.20 no.3
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• pp.301-310
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• 1992

This work was performed to investigate the possibility of using J96 hemolysin(Hly, Hly A) vaccine against urinary tract infecting Escherichia coli. Based on the known sequence of J96 hemolysin which was originally isolated from a pyelonephritis patient, ten 20-mer oligonucleotide probes were synthesized. Radioactive labelled 8 probes showed positive colony blots against most of the hemolysin producing wild type E. coli, while HA484 and HA661 showed 28.3, 71.7% positive blots, respectively. This result means that hemolysin genes are highly conserved. Also, 12 anti-Hly MABs(monoclonal antibodies) showed more than 90% positive immunoblots against secreted hemolysin from wild type E. coli. Especially, the result that MAB132 neutralized hemolysin from all of the wild type E. coli augments the idea that hemolysin will be effective as a vaccine.

• Archives of Pharmacal Research
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• v.29 no.8
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• pp.712-719
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• 2006

In this study, we prepared core-shell type nanoparticles of a poly(DL-lactide-co-glycolide) (PLGA) grafted-dextran (DexLG) copolymer with varying graft ratio of PLGA. The synthesis of the DexLG copolymer was confirmed by $^1H$ nuclear magnetic resonance (NMR) spectroscopy. The DexLG copolymer was able to form nanoparticles in water by self-aggregating process, and their particle size was around $50\;nm{\sim}300\;nm$ according to the graft ratio of PLGA. Morphological observations using a transmission electron microscope (TEM) showed that the nanoparticles of the DexLG copolymer have uniformly spherical shapes. From fluorescence probe study using pyrene as a hydrophobic probe, critical association concentration (CAC) values determined from the fluorescence excitation spectra were increased as increase of DS of PLGA. $^1H-NMR$ spectroscopy using $D_2O$ and DMSO approved that DexLG nanoparticles have core-shell structure, i.e. hydrophobic block PLGA consisted inner-core as a drug-incorporating domain and dextran consisted as a hydrated outershell. Drug release rate from DexLG nano-particles became faster in the presence of dextranase in spite of the release rate not being significantly changed at high graft ratio of PLGA. Core-shell type nanoparticles of DexLG copolymer can be used as a colonic drug carrier. In conclusion, size, morphology, and molecular structure of DexLG nanoparticles are available to consider as an oral drug targeting nanoparticles.

• Journal of Internet Computing and Services
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• v.23 no.5
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• pp.79-85
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• 2022

Anomaly detection is a method to detect and block abnormal data flows in general users' data sets. The previously known method is a method of detecting and defending an attack based on a signature using the signature of an already known attack. This has the advantage of a low false positive rate, but the problem is that it is very vulnerable to a zero-day vulnerability attack or a modified attack. However, in the case of anomaly detection, there is a disadvantage that the false positive rate is high, but it has the advantage of being able to identify, detect, and block zero-day vulnerability attacks or modified attacks, so related studies are being actively conducted. In this study, we want to deal with these anomaly detection mechanisms, and we propose a new mechanism that performs both anomaly detection and classification while supplementing the high false positive rate mentioned above. In this study, the experiment was conducted with five configurations considering the characteristics of various algorithms. As a result, the model showing the best accuracy was proposed as the result of this study. After detecting an attack by applying the Extra Tree and Three-layer ANN at the same time, the attack type is classified using the Extra Tree for the classified attack data. In this study, verification was performed on the NSL-KDD data set, and the accuracy was 99.8%, 99.1%, 98.9%, 98.7%, and 97.9% for Normal, Dos, Probe, U2R, and R2L, respectively. This configuration showed superior performance compared to other models.

• Journal of the Korean Institute of Intelligent Systems
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• v.18 no.5
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• pp.617-623
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• 2008

This paper presents a human motion recognition method using both centroid shift and local basis images. The centroid shift based on 1st moment balance technique is applied to get the robust motion images against position or size changes, the extraction of local basis images based on independent component analysis(ICA) is also applied to find a set of statistically independent motion features, which is included in each motions. Especially, ICA of fixed-point(FP) algorithm based on Newton method is used for being quick to extract a local basis images of motions. The proposed method has been applied to the problem for recognizing the 160(1 person * 10 animals * 16 motions) sign language motion images of 240*215 pixels. The 3 distances such as city-block, Euclidean, negative angle are used as measures when match the probe images to the nearest gallery images. The experimental results show that the proposed method has a superior recognition performances(speed, rate) than the method using local eigen images and the method using local basis images without centroid shift respectively.

• Journal of Society of Preventive Korean Medicine
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• v.12 no.2
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• pp.51-59
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• 2008

Objectives : Buplueri Radix (BR), used medical plant in Korea traditional medicine, contains various compounds, including a series of triterpene saponins known as saikosaponins. We performed this study for the protective effect of BR against oxidative damage induced by 1,2,4-benzentriol(BT) in human lymphocytes. Methods : In order to investigate the protective effect of BR against carcinogens, genotoxicity induced by benzene metabolite, BT were performed using cytokinesis-block micronucleus(CBMN) assay and comet assay. Results : The frequency of micronucleus at 25, 50 and $100{\mu}M$ concentration of BT were $8{\pm}2.36$, $23{\pm}2.31$, $35{\pm}4.17$ respectively. In addition of BR with concentration of 25 and $50{\mu}g/mL$, MN frequencies were significantly decreased. According to comet assay, BT induced DNA damage in a dose-dependent manner at concentration of 10 and 50 while BT with BR treatment decreased DNA breakage. No genotoxicity was observed by BR($25{\sim}50{\mu}g/mL$) treatment alone on DNA breakage. Since BT can induce DNA damage through the generation of reactive oxygen species(ROS), we examined the level of ROS in human lymphocytes treated with BT and/or BR using DCF-DA, ROS-sensitive probe. The generation of ROS in BT-treated cells was also observed, and BR addition inhibited the level of BT-induced DNA damage. Conclusions : From above results it is suggested that BR could protect the cell and DNA from pro-oxidant effect by ROS by BT

• Korean Journal of Optics and Photonics
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• v.21 no.3
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• pp.123-128
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• 2010

Sum frequency generation was utilized to obtain a yellow laser with the wavelength of 578.4 nm for a probe laser of an Yb lattice clock. The output of an Nd:YAG laser with wavelength of 1319 nm and that of an Yb-fiber laser with wavelength of 1030 nm were passed through a waveguided periodically-poled lithium niobate (WG-PPLN) for sum frequency generation. It is required that the probe laser has a linewidth of the order of 1 Hz to fully resolve the Yb lattice clock transition. Thus, the linewidth of the probe laser was reduced by stabilizing the frequency to a super-cavity. This was made of ULE with a low thermal expansion coefficient, and was mounted on an active vibration-isolation table at the optimal point for the reduced sensitivity to vibration. Also, this was installed in a vacuum chamber, and the temperature was stabilized to 1 mK level. This system was installed in an acoustic enclosure to block acoustic noise. The finesse of the super-cavity was measured to be 380 000 from the photon life time of the cavity.

• Magazine of the Korean Society of Agricultural Engineers
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• v.36 no.2
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• pp.123-131
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• 1994

Experimental studies were conducted to make clear the soil moisture environment under the condition of paddy-upland rotational fields by investigating water-table depths and soil moisture contents during growing season of crops in two kinds of soil. The following results were obtained. 1.Although water-table depths fluctuated with the amount of rainfall in the experimental field, it seemed that the variation of vater4able depths in the paddy-upland rotational, field was strongly affected by the condition of locations on paddy fields. 2.It is recognized that the concept of sum of excess water depth(SEWxx) and sum of excess water day(SEDxx) can be used to represent the soil moisture stress index due to the fluctuation of water-table depths. 3.The results of this study clearly indicate that drainage in paddy-upland rotational field to maintain an optimum soil moisture content must be made by introducing the concept of block drainage which needs both subsurface drainage and intercept drainage around a field. 4.Soil moisture contents were affected by both the amount of rainfall and water-table depths, however, the moisture content for top soil showed higher correlation with the amount of rainfall while that for subsoil with water-table depths.

• Macromolecular Research
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• v.13 no.5
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• pp.373-384
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• 2005

Novel pH-sensitive moieties containing an s-triazine ring were synthesized with sulfonamide and secondary amino groups. The synthesized pH-sensitive moieties were used for the synthesis of a pH-sensitive amphiphilic ABA triblock copolymer. The pH-sensitive triblock copolymer was composed of diblock copolymers, methoxy poly(ethylene glycol)-poly ($\varepsilon$-caprolactone-co-D,L-lactide) (MPEG-PCLA), and pH-sensitive moiety. These copolymers could be dissolved molecularly in both acidic and basic aqueous media at room temperature due to secondary amino and sulfonamide groups. The synthesized s-triazine rings containing pH-sensitive compounds were characterized by ${^1}H-NMR,\;{^13}C-NMR$, and LC/MSD spectral data. The synthesized diblock and triblock copolymers were also characterized by ${^1}H-NMR$ and GPC analyses. The critical micelle concentrations at various pH conditions were determined by fluorescence technique using pyrene as a probe. Furthermore, the micellization and demicellization study of the triblock copolymer was done with pH-sensitive groups. The sensitivity towards pH change was further established by acid-base titration.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.7
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• pp.149-159
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• 1998

The importance of soundness and safety evaluation in weld zone using by the ultrasonic wave has been recently increased rapidly because of the collapses of huge structures and safety accidents. Especially, the ultrasonic method that has been often used for a major non-destructive testing(NDT) technique in many engineering fields plays an important role as a volume test method. Hence, the defecting any defects of weld Bone in austenitic stainless steel type 304 using by ultrasonic wave and neural network is explored in this paper. In order to detect defects, a distance amplitude curve on standard scan sensitivity and preliminary scan sensitivity represented of the relation between ultrasonic probe, instrument, and materials was drawn based on a quantitative standard. Also, a total of 93％ of defect types by testing 30 defect patterns after organizing neural network system, which is learned with an accuracy of 99％, based on ultrasonic evaluation is distinguished in order to classify defects such as holes or notches in experimental results. Thus, the proposed ultrasonic wave and neural network is useful for defect detection and Ultrasonic Non-Destructive Evaluation(UNDE) of weld zone in austenitic stainless steel 304.