• Journal of Plant Biotechnology
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• v.6 no.1
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• pp.15-19
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• 2004

The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

• Proceedings of the KIEE Conference
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• 1999.07d
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• pp.1590-1592
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• 1999

We have fabricated good quality superconducting $YBa_2Cu_3O_{7-{\delta}}$ thin films on Hastelloy(Ni-Cr-Mo alloys) with $CeO_2$ buffer layers by in-situ pulsed laser deposition in a multi-target processing chamber. Using one of electrical properties of YBCO superconducting which the resistance approaches to zero dramatically on transition temperature, we have researched to make power transmission line, we have deposited YBCO thin film on flexible metallic substrate. However, it is difficult to make films on flexible metallic substrates due to both interdiffusion problem between metallic substrate and superconducting layer and non-crystallization of YBCO on amorphous substrate. From early research, two ways-using textured metallic substrate and buffer layer-were proposed to overcome theses difficulties. We have chosen $CeO_2$ as a buffer layer which has cubic structure of $5.41{\AA}$ lattice parameter and only 0.2% of lattice mismatch with $3.82{\AA}$ of a-axis lattice parameter of YBCO on (110) direction of $CeO_2$. In order to enhance the crystallization of YBCO films on metallic substrates we deposited $CeO_2$ buffer layers at varying temperature $700^{\circ}C$ to $800^{\circ}C$ and $O_2$ pressure. By X-ray diffraction, we found that each domination of (200) and (111) orientations were strongly relied upon the deposition temperature in $CeO_2$ layer and the change of the domination of orientation affects the crystallization of YBCO upper layer.

• Geotechnical Engineering
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• v.12 no.6
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• pp.115-126
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• 1996

The purpose of 1,his study is to analyze the compression and strength charactefistics of the decomposed -weathered soil originating from biotite gneiss or fine grained gneiss sampled from Poidong, Seoul : to figure out the behavioural characteristics of the decomposed -weathered soil in accordance with mineral composition and origin by comparing experimental results of residual soils. originating from granites and sampled from Bulam, Andong and Kimchun area. A series of CIU, CID CKoV, CKoD tests were car lied out. Although weathered soils have different origin and mineral composition, the slope of the NCL A was similar. It was also shown that plastic strain ratio was about 85% mainly due to the particle crushing effect during compression. The Poidong soil showed strain softening phenomenon unlike the Kimchun and Andong soils. this implies that the behavioural characteristics are affected by the origin and the mineral composition of the soil particles. Moreover, it was found that the angle of the shear resistance$(\phi')$ was dependent on the mineral composition. On the oher hand, measured Af values of decomposed weathered soils were more than one regardless of the origin and the mineral composition.

• Journal of the Korean Geotechnical Society
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• v.15 no.4
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• pp.133-152
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• 1999

The cyclic triaxial test was carried out to research liquefaction characteristics and sample disturbance effects of silty sand soils at the west coast in Korea. First, liquefaction in silty sand was generated when axial strain approached to $\pm10%$ of strain and behavior of pore pressure was similar to the formula suggested by Seed, Martin, and Lysmer(1975). Also, it was found that dilatancy was generated at failure. Secondly, the liquefaction evaluation methods suggested by many researchers were carried out and the results were compared. In these methods the weak depth in liquefaction was similar and the method carried out by cyclic triaxial test on remolded sample showed the least safety factor. Thirdly the stress ratio by cyclic triaxial test was compared with that obtained from SPT N-value as a kind of empirical methods. It was found that the effect of sample disturbance was relatively small when SPT N-value was less than 20, but there were large differences in safety factor and resistance of liquefaction in soil by the effects of disturbance and remolding when SPT N-value was more than 20.

• Proceedings of the Korea Association of Crystal Growth Conference
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• 2000.06a
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• pp.195-214
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• 2000

The high-speed steel (shorten as HSS) consists of Fe and several kinds of transition metal carbides. The cutting tools or wear-resistant materials made from HSS experience relatively high thermal shock because a coolant such as water or oil is flowed over the surface of heated HSS. The purpose of this research is to increase the hardness, strength, fracture toughness and thermal shock resistance of HSS. A possible strategy is to incorporate a hard ceramic material with high strength in HSS matrix. This paper describes the processing, microstructure and mechanical properties of the oriented unidirectional mullite fiber/HSS composite. The unidirectional mullite fibers of 10${\mu}{\textrm}{m}$ diameter were dispersed by the ultrasonic irradiation of 38 kHz in an ethylenglycol suspension containing HSS powder of 11${\mu}{\textrm}{m}$ median size. The dried green composites with 4-68 vol% fibers were hot-pressed for 2h at 100$0^{\circ}C$ in Ar atmosphere under a pressure of 39 MPa. The higher density was achieved in the composite with a lower content of fibers. The oriented unidirectional fibers were well dispersed in the HSS matrix. The average distance between the center of fibers in the cross section was close to the value calculated from the fiber fraction. No reaction occurred at the interfaces between HSS and mullite fibers in the composites. The composite with 13.6 vol% fibers showed 100 MPa of four point flexural strength at room temperature. The thermal expansion of composite with heating was influenced by the orientation of mullite fibers.

• Journal of the Korea Concrete Institute
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• v.20 no.1
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• pp.89-97
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• 2008

By replacing the meta-kaolin with cement and the waste tire chip with fine aggregate separately, the high strength concrete is protected from the spalling in fire and the method to constrain the temperature increase of steel bar within the concrete and the basic properties of the high strength concrete mixed with the material are reviewed. As the result, meta-kaolin increases the self fire proof characteristics of the concrete, the waste tire chip can share the internal expanding pressure so it can be deleted. In detail, using the meta-kaolin about the cement in 4$\sim$8% of weight ratio about the cement and the waste tire chip under the grade scope of 0.6$\sim$3 mm in 5$\sim$10% of weight ratio about the sand is very effective to prevent the spalling.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.23 no.8
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• pp.651-654
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• 2010

This paper describes the thermal and mechanical properties of doped thin film 3C-SiC and porous 3C-SiC. In this work, the in-situ doped thin film 3C-SiC was deposited by using atmospheric pressure chemical vapor deposition (APCVD) method at $120^{\circ}C$ using single-precursor hexamethyildisilane: $Si_2(CH_3)_6$ (HMDS) as Si and C precursors. 0~40 sccm $N_2$ gas was used as doping source. After growing of doped thin film 3C-SiC, porous structure was achieved by anodization process with 380 nm UV-LED. Anodization time and current density were fixed at 60 sec and 7.1 mA/$cm^2$, respectively. The thermal and mechanical properties of the $N_2$ doped porous 3C-SiC was measured by temperature coefficient of resistance (TCR) and nano-indentation, respectively. In the case of 0 sccm, the variations of TCR of thin film and porous 3C-SiC are similar, but TCR conversely changed with increase of $N_2$ flow rate. Maximum young's modulus and hardness of porous 3C-SiC films were measured to be 276 GPa and 32 Gpa at 0 sccm $N_2$, respectively.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.836-842
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• 2011

The lateral stability of bridge foundations against train moving load, emergency stopping load, earthquakes, and so on is very important for a railway bridge foundation. A borehole test is much more accurate than laboratory tests since it is possible to minimize the disturbance of ground conditions on the test site. The representative borehole test methods are Dilatometer, Pressuremeter and Lateral Load Tester, which usually provide force-resistance characteristics in elastic range. In order to estimate P-y curves using those methods, the non-linear characteristics of soil which is one of the most important characteristics of the soil cannot be obtained. Therefore, P-y curves are estimated usually using elastic modulus ($E_O$, $E_R$) of lateral pressure-deformation ratio obtained within the range of elastic behavior. Even though the pile foundation is designed using borehole tests in field to increase design accuracy, it is necessary to use a higher safety factor to improve the reliability of the design. A Large Displacement Borehole Testmeter(LDBT) is developed to measure nonlinear characteristics of the soil in this study. P-y curves can be directly achieved from the developed equipment. Comparisons between measured P-y curves the LDBT developed equipment, theoretical methods based on geotechnical investigations, and back-calculated P-y curves from field tests are shown in this paper. The research result shows that the measured P-y curves using LDBT can be properly matched with back-calculated P-y curves from filed tests by applying scale effects for sand and clay, respectively.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.4
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• pp.965-975
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• 1994

Opening the tip of a PHC pile, under a constant driving energy, can result in an increment of penetration depth due to the decrement of driving resistance. Therefore, the bearing capacity of an open-ended PHC pile may become larger than that of a closed-ended PHC pile by virtue of the increased embedded length. However, two main problems can be caused by opening the end of PHC pile. First problem is the variation of bearing capacity by opening the pile tip, and the second one is whether the tip of an open-ended PHC pile will be failured by a high pressure developed by the soil plug. In this study, model pile tests in calibration chamber were performed to investigate the practicability of open-ended PHC pile in view of both the pile bearing capacity and the possible failure of the pile tip. According to the test results, the total bearing capacity of open-ended piles approaches the total bearing capacity of closed-ended piles with the increase of the penetration depth. The failure of pile tip could be occurred in the region of 0.8~1.1 times as the inside diameter from the pile tip.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.12
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• pp.1267-1271
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• 2012

Demand for optical glass device used for lighting could increase rapidly because of LED lighting market growth. The optical glass devices that have been formed by hot press molding process the desired optical performance without being subjected to mechanical processing such as curve generation or grinding. EL-Max material has been used for many engineering applications because of their high wear resistance, high compressive strength, corrosion resistant and very good dimensional stability. EL-Max is very useful for a glass lens mold especially at high temperature and pressure. The performance and reliability of optical components are strongly influenced by the surface damage of EL-Max during grinding process. Therefore, the severe process condition optimization shall be necessary for the highly qualified EL-Max glass lens mold. To get the required qualified surface of EL-Max, the selection of type of the diamond wheel is also important. In this paper, we report best grinding conditions of ultra-precision grinding machining. The grinding machining results of the form accuracy and surface roughness have been analyzed by using Form Talysurf and NanoScan.