• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.293-296
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• 2002

Hyperosmotic pressure increased specific antibody productivity ($q_{Ab}$) of recombinant CHO cells (SH2-0.32) while it depressed cell growth. Thus, the use of hyperosmolar medium did not increase the maximum antibody concentration substantially. To overcome this drawback, the feasibility of biphasic culture strategy was investigated. In the biphasic culture, cells were first cultivated in the standard medium with physiological osmolality(294 mOsm/kg) for cell growth. When cells reached the late exponential phase of growth, the spent standard medium was replaced with the fresh hyperosmolar medium (522 mOsm/kg) for antibody production. The ($q_{Ab}$) in growth phase with the standard medium was 2.1 ${\mu}g/10^6cell/day$ while the ($q_{Ab}$) in antibody production phase with the hyperosmolar medium (522 mOsm/kg) was 11.1 ${\mu}g/10^6cell/day$. Northern blot analysis showed a positive relationship between the relative contenet of Ig mRNA and ($q_{Ab}$), indicating that transcriptional regulation was involved in the response of rCHO cells to hyperosmotic pressure. Due to the enhanced ($q_{Ab}$) and increased cell concentration in biphasic culture, the maximum antibody concentration obtained in biphasic culture with 522 mOsm/kg medium exchange was 161% higher than that obtained in batch culture with the standard medium. Taken together, simple biphasic culture strategy based on hyperosmotic culture for improved foreign protein production from rCHO cells is effective in improving antibody production of rCHO cells.

• Journal of Advanced Marine Engineering and Technology
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• 제40권4호
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• pp.295-300
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• 2016

P사의 실린더 당 210 kW 급 중속디젤엔진의 연료분사펌프를 대상으로 연료 송출 압력파에 대해서 Ansys Fluent R15.0을 사용하여 수치 해석하였다. 연료 송출 압력파의 실험 결과와 수치 해석 결과가 유사하게 나타났으며, 전산유체역학을 이용한 연료분사펌프의 연료 송출 압력파에 대한 수치 해석의 신뢰성을 확인하였다.

• Current Optics and Photonics
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• 제2권3호
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• pp.250-260
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• 2018

A pressure wave created by the photoacoustic effect is affected by the medium and by laser parameters. The effect of these parameters on the generated pressure wave can be seen by solving the photoacoustic wave equation. These solutions which are examined in the time domain and the frequency domain should be considered by researchers in acoustic sensor design. In particular, frequency domain analysis contains significant information for designing the sensor. The most important part of this information is the determination of the operating frequency of the sensor. In this work, the laser parameters to excite the medium, and the acoustic signal parameters created by the medium are analyzed. For the first time, we have obtained solutions for situations which have no frequency domain solutions in the literature. The main focal point in this work is that the frequency domain solutions of the acoustic wave equation are performed and the effects of the frequency analysis of the related parameters are shown comparatively from the viewpoint of using them in acoustic sensor designs.

• 한국광물학회지
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• 제30권3호
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• pp.83-91
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• 2017

천연산 녹주석($Be_3Al_2Si_6O_{18}$, P6/mcc) 중 산출지가 각각 다른 아쿠아마린 시료인 녹주석-A, 녹주석-B를 물을 압력전달 매개체로 사용하여 고압실험 및 고온-고압실험을 실시하였다. 기존 문헌과 다르게 압력전달 매개체로 물을 이용했을 때 고압 및 고온-고압 상태에서 녹주석의 조성과 구조에 어떠한 영향을 주며 탄성 특성이 어떻게 변하는지를 확인하기 위함이다. 그 결과 녹주석-A, B의 체적탄성률은 각각 111(7) GPa, $K{_0}^{\prime}=73(7)$; 110(9) GPa, $K{_0}^{\prime}=65(8)$로 확인되었다. 이는 기존 연구에서 메탄올 에탄올 4 : 1 체적비로 혼합하여 관찰한 것과 다른 값 및 경향성을 보여주는 것으로 확인하였으며 관찰된 녹주석의 치밀화는 ICE VI, ICE VII 상변이 구간인 약 1.0 GPa, 약 2.5 GPa 구간과 일치하였다.

• 상하수도학회지
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• 제17권4호
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• pp.542-549
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• 2003

In the present study, the feasibility of $UV/H_2O_2$ systems was investigated using low and medium-pressure lamps on biologically treated wastewater effluents for secondary effluent reclamation. Two types of UV lamps were used as the light sources (a 39-W low-pressure mercury lamp and a 350-W medium-pressure mercury lamp). The results from these UV systems showed that the removal of organic compounds could be achieved in the contact time of longer than 30min (i.e., low UV doses). Efficiencies of color removal and disinfection were far better than those of organic matters measured as TOC, DOC and $TCOD_{cr}$. In the low-pressure lamp UV system, it has been found that DOC and color removals were 60.9 and 86.2% with 50mg/L of $H_2O_2$ and contact times of 30 minute, respectively. Whereas, with the medium-pressure lamp UV system, TOC, DOC and color removal were 27.1, 5.6 and 95% with 14.3mg/L of $H_2O_2$ and 14 minute of contact times, respectively. Both systems could be applied for the reclamation of secondary effluent treated with biological treatment processes.

• Journal of Biosystems Engineering
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• 제34권4호
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• pp.211-219
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• 2009

In the process of oyster mushroom production, loading work of culture medium needs the most intensive labor power and cost. Therefore, the development of culture medium loading machine causes to reduce the manpower and cost. The main objectives of this study are identify cultivating environment, physical properties of culture medium and to make an element design of culture medium loading machine. The results are summarized as follows: 1. The moisture content and density of popularly used culture medium were 70%(w.b), $26\;kg/m^3$, respectively. 2. Pressure of the blower increased as the impeller speed increased, and the opening ratio of pressure controller decreased. 3. Recommendable c1earance(${\delta}$) between an impeller plate and a blower case was 25 mm at an impeller speed of 3183 rpm 4. Discharge device of type B with a hopper and suit was better than type A with a hopper.

• 한국세라믹학회지
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• 제36권11호
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• pp.1189-1197
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• 1999

Low-density silica gel monolith was synthesized at ambient pressure by surface modification with TMCS and sub-sequent heat treatment. The mechanical thermal and optical properties of gel were studied. Compressive strength and modulus of compression of 350$^{\circ}C$-heated gel with the density of 0.24g/cm3 were 250kPa and 0.2MPa respectively. The thermal conductivity of silica gels synthesized at ambient pressure exhibited 0.02 W/m$.$K for the density of 0.24g/cm3 which is similar to that of the gel via supercritical drying and their main thermal transfer mechanism is considered to be solid and radiation conduction at room temperature. Ambient-dried silica gels were transparent blue showing about 60% of transmittance in the wavelength of 1500-2100nm and typical absorption bands of existing bonds under heat treatment at 350$^{\circ}C$. Medium scale monolity(${\Phi}$=50mm) at ambient pressure could be successfully prepared through total 5-month process period.

• 한국안전학회지
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• 제16권4호
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• pp.39-46
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• 2001

It is noted that KS D 3507 pipe steel has several problems when it is used as a city gas pipe at medium pressure. So new pipe steel, KS D 3631, was developed in order to be used as a pipe for medium and low pressure and now it is being substituted for D 3507. In this study, several mechanical tests, such as tensile test, microhardness test, and Charpy impact test were conducted to get material properties of D 3507 and D 3631 pipe steels. And also microstructures at the weld and heat affected zones were observed for the two materials. From the Charpy test results $K_{IC}/$ was estimated for the upper and lower shelf and the critical crack length is calculated for supposed axial semi-elliptical surface cracks. And the burst pressure is estimated as a function of wear depth for a defective D 3631 pipe by using the finite element method. The burst pressure is also calculated for pipes with an axial crack by using the published equations.

• 조명전기설비학회논문지
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• 제27권10호
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• pp.86-92
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• 2013

In studies on an alternative insulating gas of $SF_6$ gas, the section of the alternative gas and an insulation technique to improve its low dielectric strength have been reported, but very few attempts have been made at the dependence of a gas pressure and a gap as well as the medium effect in the alternative gas. The purpose of this paper is to analyze the dependence of the gas pressure and the gap at surface flashover voltage in dry air. The dependence is analyzed based on the medium effect. The medium effect by the gas pressure and the gap can be explained by surface roughness of a solid dielectric and an electrode as well as an electric field which decreases due to the correlation between the collision ionization coefficient and the gap, respectively. In addition, an insulation technique which can fabricate a compact eco-friendly gas insulated switchgear is proposed by the results of this paper.

• 한국직업건강간호학회지
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• 제4권호
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• pp.30-38
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• 1995

The purpose of this study was to evaluate the effect of health services on workers with hypertension in small and medium sized enterprises (20-300 employees). One hundred seventy three workers among 296 workers with hypertension were responded to the questionnaire on general and occupational characteristics, health habits and anti-hypertensive medications. Blood pressure was obtained while seated using a calibrated standard manometer before and after 4 months health services. Statistical analysis was performed using $X^2$-test and logistic regression analysis. The results were as follows ; 1. No significant differences were noted in variables of smoking, glucose intake and sleeping time, while significant differences were showed in variables of exercise time, frequency and amount of drinking and intake of salt, protain, lipid, calcium, vegetable and fruits between groups which was provided and not provided health services. 2. The rate of anti-hypertensive medication was increased and the blood pressure was decreased significantly in group which was provided health services compared with those of group which was not provided. 3. With the logistic regression analysis, significant variables affecting blood pressure change were smoking and anti-hypertensive medication. The results of this study suggested that health services on workers with hypertension in small and medium sized enterprises was effective, and the role of health professionals for health promotion movement in enterprises was important.