• Archives of Pharmacal Research
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• 제22권3호
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• pp.288-293
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• 1999

Postabsorptive serum iron level was determined after oral administration of the compounds to human. In serum and whole blood, $Fe^{3+}$ was measured by ion chromatography (IC) using a pyridine-2,6-dicarboxylic acid (PDCA) as an eluent. The serum sample solutions were pretreated with I N HCI and 50% TCA. The whole blood sample solutions were treated with 3 N HCI for 30 min at $125^{\circ}C$. The limit of detection (LOD) of the IC technique is $0.2 {\mu}M$ for$Fe^{2+}$and 0.1 $\mu$M for $Fe^{3+}$. The area under concentration (AUC) can be obtained by the above analytical condition. In addition, to compare the stability of $Fe^{2+}$ to that of $Fe^{3+}$ in pharamaceutical preparations, accelerated stability test was carried out. After storing the samples under $40^{\circ}C$, 75%RH in light-resistant container for various time intervals, the contents of iron of different valencies were determined separately by the IC technique and the change and/or the interchange of among those iron species in preparations was investigated. Iron raw materials are stable, but $Fe^{2+}$ in$Fe^{3+}$ source materials was slightly converted to $Fe^{3+}$ by oxidation. $Fe^{2+}$ in$Fe^{3+}$ source raw materials and $Fe^{3+}$ in $Fe^{2+}$ raw materials are determined as impurities. Therefore, IC technique is found to be an appropriate method for comparative evaluation of dissimilar bioavailability of $Fe^{2+}$ and $Fe^{3+}$, stability of $Fe^{2+}$ and $Fe^{3+}$ raw materials and preparations.

• 한국환경보건학회지
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• 제45권2호
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• pp.154-163
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• 2019

Objectives: The hazardous heavy metal contents of cosmetics were investigated and the resulting values were compared by type of cosmetics: skin care preparations (SCP), hair preparations (HP), makeup preparations (MP), and eye makeup preparations (EMP). Methods: The hazardous heavy metal contents (Pb, As, Cd, Sb, Ni and Hg) were analyzed for 358 cosmetics products (187 SCP, 82 HP, 56 MP, and 33 EMP). Hg was measured by the amalgamation method, and other hazardous heavy metals were measured by inductively coupled plasma optical emission spectrometer (ICP-OES) after decomposition using the microwave method. Results: The mean contents of Pb, As, Cd, Sb, Ni, and Hg in cosmetics were 0.424, 0.068, 0.024, 0.398, $0.567{\mu}/g$, and Not Detected, respectively. All of the hazardous heavy metals were detected in most products, but below the recommended maximums of the Ministry of Food and Drug Safety. The level of Cd was the lowest at 14.8%, and Sb was the highest at 41.2%. Pb, Sb and Ni showed the highest mean value and detection rate in EMP. As, Cd, and Hg showed the highest in SCP, HP, and MP, respectively. Conclusion: Hazardous heavy metals were detected in most products. In particular, Pb, Sb, and Ni were broadly detected in EMP, meaning more stringent quality control is required.

• 공업화학
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• 제22권1호
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• pp.91-97
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• 2011

이 연구는 아토피 피부염이 유발된 hairless mice에 항 아토피 제품 처리를 통해 아토피 증상 개선 효과에 대해 알아 본 실험이다. 항 아토피 물질인 ceramide와 astaxanthin을 melt-homogenization 방법을 이용해 고형 지질 나노입자를 제조하고 입자 주위에 silk fibroin을 코팅시켰다. 동시에 고형 지질 나노입자를 함유하는 항 아토피 로션(lotion)과 크림(cream)을 만들어 타 사의 항 아토피 제품과 비교하였다. 아토피 피부염이 유발된 마우스의 외형적인 모습 관찰을 통해 ceramide와 silk fibroin을 함유한 항 아토피 제품인 FL과 FC는 아토피 피부염으로 손상된 피부 장벽을 재생 시키는 것을 확인하였고, 아토피 피부염이 유발된 피부 두께 역시 감소됨을 알 수 있었다. 면역학적 평가를 통해 silk fibroin과 astaxanthin 이 Th2 cytokine인 IgE와 IL-4의 발현을 억제하고 $IFN-{\gamma}$의 발현을 증가시켜 Th cell 사이의 균형을 이루게 하는 것이 밝혀졌다. 또한, 비장수치를 통해 T 림파구의 따른 IgE, IL-4 발현 억제능, 그리고 $IFN-{\gamma}$ 발현을 확인하였다.

• 대한본초학회지
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• 제37권5호
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• pp.75-81
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• 2022

Objectives : In 『Treatise on Cold Damage Diseases』, the volume of preparation dosage measured in Hap was 6.5mL. This study aimed to confirm that a square-inch-spoon, which measures powder preparation dosage, was 1/3 Hap, and that Jeon-bi was 1/8 square-inch-spoon. Based on these values, this study also aimed to adjust the diameter of Firmiana-Seed-sized pill preparation, currently known as 6.5mm, so that one square-inch-spoon of powder preparation creates 16 pills. Methods : Calculated the ratio between the floor areas of square-inch-spoon and Jeon-bi and measured the ratio of volume; assumed that powder preparations were stacked as much as possible in the shape of a square pyramid when scooped comfortably, then calculated the slopes of 1/3 Hap and 1/4 Hap; measured the volumes of powdered Oryeongsan and Lijungwhan in square-inch-spoon and Jeon-bi; created the powders into Firmiana-Seed-sized pill preparation; adjusted the diameter of Firmiana Seed so that 16 pills could be created. Results : Inferring from the floor areas, the volume of square-inch-spoon is 9.2 times that of Jeon-bi; one square-inch-spoon should be 1/3 Hap for the powder to reach its maximum slope of 47° and, according to actual measurement of powder preparations, reach its maximum volume; when created into Firmiana-Seed-sized pill preparations, one square-inch-spoon should be 1/3 Hap so that approximately 16 pills were created. Conclusions : One square-inch-spoon of powder preparation was 1/3 Hap(2.17mL), Jeon-bi was 1/8 square-inch-spoon(0.27mL), and the diameter of Firmiana-Seed-sized pill preparation was 5.5mm.

• Korean Circulation Journal
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• 제53권4호
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• pp.239-250
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• 2023

Background and Objectives: Brugada syndrome (BrS) is an inherited arrhythmia syndrome that presents as sudden cardiac death (SCD) without structural heart disease. One of the mechanisms of SCD has been suggested to be related to the uneven dispersion of transient outward potassium current (I_to) channels between the epicardium and endocardium, thus inducing ventricular tachyarrhythmia. Artemisinin is widely used as an antimalarial drug. Its antiarrhythmic effect, which includes suppression of I_to channels, has been previously reported. We investigated the effect of artemisinin on the suppression of electrocardiographic manifestations in a canine experimental model of BrS. Methods: Transmural pseudo-electrocardiograms and epicardial/endocardial transmembrane action potentials (APs) were recorded from coronary-perfused canine right ventricular wedge preparations (n=8). To mimic the BrS phenotypes, acetylcholine (3 μM), calcium channel blocker verapamil (1 μM), and I_to agonist NS5806 (6-10 μM) were used. Artemisinin (100-150 μM) was then perfused to ameliorate the ventricular tachyarrhythmia in the BrS models. Results: The provocation agents induced prominent J waves in all the models on the pseudo-electrocardiograms. The epicardial AP dome was attenuated. Ventricular tachyarrhythmia was induced in six out of 8 preparations. Artemisinin suppressed ventricular tachyarrhythmia in all 6 of these preparations and recovered the AP dome of the right ventricular epicardium in all preparations (n=8). J wave areas and epicardial notch indexes were also significantly decreased after artemisinin perfusion. Conclusions: Our findings suggest that artemisinin has an antiarrhythmic effect on wedge preparation models of BrS. It might work by inhibition of potassium channels including I_to channels, subsequently suppressing ventricular tachycardia/ventricular fibrillation.

• 한국임상약학회지
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• 제18권2호
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• pp.97-105
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• 2008

Hospital on-site preparations (HOP) have long been produced in most institutions since enactment of the Ministry of Health and Welfare Notification No. 92-12 in 1992. However, due to the unclear definition of the HOP, most institutions have trouble in production and reporting of the HOP. Therefore, in this study, the definition and range of HOP are examined by questionnaire for 34 institutions, mainly teaching hospitals, which reported the items of HOP among 84 institutions having 500 or more beds. It was found that 472 items and 1,116,888 jesu of the HOP were produced at 34 institutions in 2006. Most institutions prepared $21{\sim}30$ items of HOP, and the number of institutions producing $5,000{\sim}10,000$ forms was most prevalent. Accordingly, for HOPs whose need was identified by this study, pharmaceutical companies should be pressed for commercialization.

• 가정과삶의질연구
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• 제32권6호
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• pp.73-87
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• 2014

The study researches the actual preparations for elderhood as well as recognizes general preparations for it, and analyzes the demand for educational programs on elderly life. Married women aged 35 years to 60 years who live in Jeju province were surveyed. The data were analyzed according to frequency, t-test, and one way ANOVA analysis. The results of this study are as follows: First, most middle-aged women aren't prepared for elderhood. Second, regarding the contents of the educational programs on elderly life, it was found that the following were in high demand: that the part of life design establishes positive old-age form, maintenance of good health, investment knowledge, leisure activities, and effective communication with family members. Third, significant differences in demand for educational programson elderlylife were found according to age, the age of the last child, income, the age at which one prepares for elderhood, and the amount of funds reserved for elderhood.

• 대한약침학회지
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• 제14권2호
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• pp.5-14
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• 2011

Objectives: Fibrinolytic enzyme preparations were isolated from the snake venom of Macrovipera lebetica turanica in this study. Methods: The purity of the preparations was determined using SDS-PAGE and the enzymic characteristics of the purified fibrinolytic enzyme were determined. Results: 1. All of the two preparations with fibrinolytic activity obtained from the snake venom of M. l. turanicat contained the major polypeptide with the molecular weight of 27,500. One of the preparation showed purified fibrinolytic enzyme. 2. The purified fibrinolytic enzyme hydrolyzed ${\alpha}$-chain of fibrinogen faster than ${\beta}$-chain but not ${\gamma}$-chain. 3. The fibrinolytic activity was inhibited completely by EDTA, EGTA, 1,10-phenanthroline, and dithiothreitol. 4. The fibrinolytic activity was inhibited completely by calcium chloride, iron(III) chloride, mercuric chloride, and cobalt (II) chloride. 5. The fibrinolysis zone formed after addition of zinc sulfate was smaller but clearer than the control. Conclusions: These results suggested that the fibrinolytic enzyme purifed from the snake venom of M. l turanica was a metalloprotease containing dithiol group.

• 대한치과의사협회지
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• 제36권2호통권345호
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• pp.144-150
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• 1998

The dental insurance had been enforced in Korea past 20 years. The major part of the insurance was filling, and the filling was divided into two parts-the cavity preparation and cavity filling, The Cavity filling was estimated into 6 levels according to the cavity types. But the tooth cavity preparations was not evaluated by the cavity types but merely only one-the one visit filling or cavity preparation. Korean dentists tend to turn away from the amalgam to the non-insurance treatments. The purpose of this study was to relate the cavity preparations and cavity fillings to the cavity types. So I measured the real time and surveyed the relative difficulties of cavity preparations from the 31 dentists according to the cavity types, who had been trained in conservative dentistry. The conclusion of this study was below. 1. The estimations of tooth cavity preparation was divided same as the cavity filling. 2. There must be reasonable considerations about the times and difficulties in cavity preparation. 3. There was differences between the premolars and molars, upper jaw and lower one. 4. Two or more cavities in one tooth must be evaluated according to the number of cavities, not in one cavity.

• Biomolecules & Therapeutics
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• 제13권1호
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• pp.59-63
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• 2005

A kinetic assay was carried out in order to compare the ability of detection for prekallikrein activator(PKA) in plasma-derived products with that of an endpoint assay and a commercial method. Using these methods, 9 human albumin preparations were assayed and compared to each other. The coefficient of variation between the Kinetic assay and the end point assay was found within 6.6% and this result showed that two methods were highly correlative and the end point assay could act as a replacement of the kinetic assay. Another important goal of this study was to investigate the reproducibility among laboratories on the kinetic assay. A collaborative study was performed to validate the kinetic method with intra and inter assays. The coefficient of variation for the intra assay of each laboratory was less than 4% and that for between individuals in the inter assay was 4.1%. These results revealed that the kinetic assay showed good reproducibility. The contents of PKA in plasma-derived products were also determined by the kinetic assay. As a result, it was found that trace amounts of PKA were present in 32 human immunoglobulin preparations, however the average concentration of PKA in 171 albumin preparations was 5.8 IU/mL.