• Title/Summary/Keyword: Predominant frequency

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Analyzing Vomit of Platalea minor (Black-faced Spoonbill) to Identify Food Components using Next-Generation Sequencing and Microscopy (차세대염기서열 및 현미경 분석을 통한 저어새의 토사물 내 먹이생물 분석)

  • Kim, Hyun-Jung;Lee, Taek-Kyun;Jung, Seung Won;Kwon, In-Ki;Yoo, Jae-Won
    • Korean Journal of Environmental Biology
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    • v.36 no.2
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    • pp.165-173
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    • 2018
  • We sampled vomit of black-faced spoonbills(Platalea minor) during the brood-rearing season (from June 2011 to June 2014) at the Namdong reservoir in Incheon and analyzed the food components in the vomit using microscopy and next-generation sequencing (NGS). Microscopic observations primarily helped in identifying osteichthyes (bony fishes), crustaceans, and polychaetes. In particular, species belonging to the families Mugilidae and Gobiidae among the fish, and Macrophthalmus japonicas among the crustaceans, were observed at high frequency. Results of NGS analysis revealed the predominant presence of bony fish (42.58% of total reads) and crustaceans (40.75% of total reads), whereas others, such as polychaetes (12.66%), insects (0.24%), and unidentified species (3.78%), occurred in lower proportions. At the species level, results of NGS analysis revealed that Macrophthalmus abbreviates and Macrobrachium sp. among the crustaceans, and Acanthogobius hasta, Tridentiger obscurus, and Pterogobius zacalles among the bony fish, made up a high proportion of the total reads. These food species are frequently found at tidal flats in the Songdo and Sihwa lakes, emphasizing the importance of these areas as potential feeding sites of the black-faced spoonbill. Feed composition of the black-faced spoonbill, as evaluated by analyzing its vomit, differed when the evaluations were done by microscopic observation or by NGS analysis. Evaluation by microscopic observation is difficult and not error free, owing to the degradation of the samples to be analyzed; however, NGS analysis is more accurate, because it makes use of genetic information. Therefore, accurately analyzing food components from morphologically indistinguishable samples is possible by using genetic analysis.

Comparative Characterization of the Bacteria Isolated from Market Milk Treated with ESL and Conventional System (ESL 생산공정에 따른 시유 유래 미생물의 분포 비교 연구)

  • 김응률;정병문;유병희;정후길;강국희;전호남
    • Food Science of Animal Resources
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    • v.23 no.4
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    • pp.327-332
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    • 2003
  • This study was conducted to investigate the characteristics of strains which were isolated from market milk treated with ESL(extended shelf life) and conventional system, and to compare the microbiological quality of ESL milk with conventional milk. In order to characterize the isolated strains, purification, Gram staining, spore staining, catalase, oxidase, motility test, and identification by means of automatic identificator were performed. The results obtained are as follows: total 364 selected strains were analyzed in this study. Depending upon the isolated source, the number of strains from conventional milk was found to be Higher than ESL-milk. By means of grouping of total strains, Bacillus ssp. and Staphylococcus ssp. showed to be predominant. But most of strains were distributed with various groups except Lactobacillus ssp. When the isolates were compared with milk process methods, Enterococcus ssp. was detected much on market milk treated with LTLT pasteurization. Also, Pseudomonas ssp. was detected much on conventional milk treated with UHT pasteurization. By comparison with genus groups depending upon storage temperature of market milk, the higher milk storage temperature increased, the most frequency detected Bacillus ssp. increased. Also, Pseudomonas ssp. was detected most frequently at 10$^{\circ}C$ storage condition. Generally this genus derived from post-contamination during milk processing and related to the quality of market milk during chilled system. In conclusion, it was shown that ESL system reduced post-contamination during milk process, following the improvement of product quality and life cycle during the distribution of market milk.

Rheological Properties of Citrus Pectin Solutions (감귤류 펙틴 용액의 리올리지 특성)

  • Hwang, Jae-Kwan
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.799-806
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    • 1995
  • The steady shear and small amplitude oscillatory dynamic rheological properties of citrus pectin $([\eta]=3.75\;dL/g)$ were characterized for a wide range of pectin concentrations $({\sim}6%)$. The typical power-law flow was observed above 2.0% concentration, and the shear rate dependence of viscosity increased with pectin concentration. The transition from dilute to concentrated regime, determined from the double logarithmic plot of ${\eta_{sp.o}}\;vs\;C[\eta]$, occurred at a critical coil overlap parameter $C^{*}[\eta]\approx4.0$, at which ${\eta_{sp.o}}$ corresponded to approximately 10.0. The slopes of ${\eta_{sp.o}}\;vs\;C[\eta]$, at $C[\eta]\;at\;C[\eta]C^{*}[\eta]$were 1.1 and 4.5, respectively. The steady viscosity $(\eta)$ displayed a good superposition at ${\eta}/{\eta}_o\;vs\;{\gamma}/{\gamma}_{0.8}$ relation with an exception of high concentration (6%), which arised from the significant deviation of flow behavior index (n values of $\eta_{a}=K\gamma^{n-1}$) at high concentration. Dynamic measurements showed that the loss modulus $(G^{\prime\prime})$ was much higher than the storage modulus $(G^\prime)$for all concentrations studied, indicating predominant viscoelastic liquid-like behavior of pectin solutions. The frequency dependence of $G^\prime$ was higher than that of $G^\prime\prime$ at the same concentration, whose trend was more pronounced with decreasing pectin concentration. The shear viscosity $(\eta)$ was almost identical to the complex viscosity $(\eta^{*})$ at low concentration, following the Cox-Merz rule, but they became increasingly different at high concentration.

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Congener Specific Profiles and Exposure Pathways of Polybrominated Diphenyl Ethers (PBDEs) in Breast Milk of Chuncheon Area (춘천지역 모유 중 폴리브롬화디페닐메테르(PBDEs)의 이성체별 분포특성과 노출경로)

  • We, Sung-Ug;Baek, In-Cheon;Park, Sang-Ah;Kang, Seong-Hoon;Cho, Yu-Jin;Cho, Bong-Hui;Yoon, Cho-Hee;Kim, Ki-Ho;Min, Byung-Yoon
    • Journal of Life Science
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    • v.20 no.3
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    • pp.356-364
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    • 2010
  • This study was carried out to investigate the exposure pathway of PBDEs in human breast milk collected from new mothers residing in Chuncheon, a comparatively clean area in Korea. The congeners of PBDE in 22 human breast milk samples were analyzed using a high resolution gas chromatography with a high resolution mass detector. The residue level of ${\sum}PBDEs$ was higher in primipara subjects than in multipara subjects (p<0.05). The levels in this study were similar to those of people in some Asian and European countries, but were lower than those of people in north America. In the congener profiles, BDE-47 (mean contribution=36.1%) was predominant, followed by BDE-153 (27%), BDE-99 (11.7%), BDE-100 (11.1%), BDE-28 (7.9%) and BDE-183 (3.5%). The sum of BDE-47 and BDE-153 accounted for more than 50% of ${\sum}PBDEs$ in most samples. BDE-47 was highly correlated with ${\sum}PBDEs$ (r=0.94, p<0.001). No strong trend was observed between PBDE levels and a number of key biological factors (women's age, weight, height and body mass index) examined in this study, however, weak correlations were observed in PBDE levels measured against dietary habits, particularly in fish consumption frequency. It seems that Korean people might be exposed to multiple sources including products of PBDEs, and particularly food resources.

Utilization of Pigments and Tunic Components of Ascidian as an Improved Feed Aids for Aquaculture 2. Chemical Properties of Sulfated Polysaccharides in Ascidian (Halocynthia roretzi) Tunic (우렁쉥이 껍질성분 및 색소를 이용한 양식소재 개발 2. 우렁쉥이 껍질 중 부분정제한 황산다당의 화학적 특성)

  • HONG Byeong-Il;JUNG Byung-Chun;JUNG Woo-Jin;RUCK Ji-Hee;CHOI Byeong-Dae;LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.6
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    • pp.632-637
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    • 2001
  • Components of polysaccharides isolated from ascidian tunic were measuerd by gel filtration, electrophoresis and chemical analyses. The sulfated polysaccharides consisted in sulfate, protein, uronic acid and amino sugars. Hexosamines were composed of arabinose, xylose, glucose, galactose, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine by gas chromatography analysis. The galactose was predominant hexose after autoclave and nutrase digestion followed by DEAE-cellulose ion exchange chromatography and gel-permeation chromatography on Sephadex G-100 and G-25. FT-IR spectra of isolated polysaccharides from ascidian tunic and standard chondroitin sulfates have similar functional groups of the type of vibration and frequency. Molecular weight of isolated polysaccharides by autoclave represented more than 40 kDa by polyacrylamide gel electrophoresis. But the neutrase treatment group divided into three band. The highest molecular band group was shown more than 100 kDa, and the two low molecular band group were shown about 22 kDa and 5 kDa, respectively, compare to standard materials.

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A Study on the Family Situation of Sterilized Homemakers (불임피술주부(不妊被術主婦)의 가정적(家庭的) 배경(背景)에 관(關)한 연구(硏究))

  • Kim, Chi-Wha
    • Clinical and Experimental Reproductive Medicine
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    • v.4 no.2
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    • pp.23-34
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    • 1977
  • A study was carried out for the evaluation on family situation of 900 homemakers those who had received tubal sterilization operation (laparoscopic and minilaparotomy) at family planning clinic, Seoul Red Cross Hospital from October 1974 to September 1977. The results obtained are as follows: 1) In age distribution, predominant age group consisted of those $31{\sim}35$ years frequency of 43.2% and average age was 33.3 years. 2) Educational level showed that homemakers who finished primary school accounted for 37.3% of the total and those having middle school education comprised 28.7%, and 24.3% of them were high school graduates, 8.3% of them were college graduates. On the other hand, husband's education level showed that, 37.6% of them were high school graduates, 29.7% were college graduates and 19.9% were middle school graduates, therefor, educational level of husbands was one step higher than wives. 3) In the gravidity at the tubal sterilization, the highest incidence(18.4%) among 853cases was the group of gravida 5, and 16.2% gravida 4, and the gravidity ranged $1{\sim}23$. Average gravidity of clients was 6.0. 4) Among the total number of 900 clients, 778cases (91.3%) had no experience of spontanous abortion, history of 1 abortion in 5.9%, 2 abortions in 1.8%, and it showed the decresed incidence of spontanous abortion recently. Average was 0.15. As regarding induced abortion, in spite of only 142 homemakers (16.7%) had no history of induced abortion, 20.5% had experienced 1 induced abortion before sterilization, 17.7% had 2 induced abortions, 14.6% had 3 abortions, 10.3% had 4 abortions, and 0.2% (2cases) had over 20 abortions. Average was 2.7. 5) In regarding to the number of living children, the greatest number (45.0%) of clients had 3 children, and 26.5% 2 children, 19.7% 4 children. Average number of their living children was 3.03. 6) Sex ratio of living children, among 18 clients those had 1 child, 17 homemakers had 1 boy and no girl, 1 homemaker had no boy and 1 girl only. Sex ratio showed that woman who had 2 boys and no girl accounted for 46.3%, however, those having no boy and 2 girls ocmprised only 1. 8% among 229 clients who had 2 children. Among 389 clients who had 3 children, in spite of woman who had 3 boys and no girl comprised 16.5%, but no boy and 3 girls only 1.5%. Among 170 clients who had 4 children, homemakers with 4 boys and no girl accounted for 4.1%, however, no boy and 4 girls 1.8% of the total. Among 52 clients, who had 5 children, woman with 5 boys and no girl comprised 3.9%, no boy and 5 girls 0%. Among 7 cases who had 6 children, there were 3 cases who had 3 boys and 3 girls, but only 1 cases had 1 boy and 5 girls and so on. These results showed a strong trend of male preference in Korea and this could be one of the inhibit factors for family planning.

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A Comparative Study on the Quality of Sleep, Tongue Diagnosis, and Oral Microbiome in Accordance to the Korean Medicine Pattern Differentiation of Insomnia (불면 변증에 따른 수면의 질, 설진, 구강 미생물 차이에 대한 비교 연구)

  • Shim, Hyeyoon;Kwon, Ojin;Kim, Min-Jee;Song, Eun-Ji;Moon, Sun-Young;Nam, Young-Do;Nam, Dong-Hyun;Lee, Jun-Hwan;Koo, Byung Soo;Kim, Hojun
    • Journal of Korean Medicine for Obesity Research
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    • v.20 no.1
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    • pp.40-51
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    • 2020
  • Objectives: We aimed to compare the quality of sleep, tongue diagnosis, oral microbiology differences in insomnia of Liver qi stagnation (LQS) and Non-Liver qi stagnation (NLQS). Methods: 56 patients were classified as LQS or NLSQ type insomnia through the insomnia differentiation questionnaire. The depression scores between the groups were compared through beck depression inventory (BDI), and the sleep quality was compared through Pittsburgh sleep quality index (PSQI) and Insomnia Severity Index (ISI). We analyzed the sleep efficiency, total sleep time, total awake frequency, total and average awake time through actigraph. For the tongue diagnosis, the distribution of tongue coating in six areas were measured through Winkel tongue coating index (WTCI). Linear discriminant analysis was performed to observe the differences in composition of microbial strains between the groups. Results: The scores of BDI, ISI and PSQI were significantly higher in LQS group. The total sleep time in LQS group was significantly less than that of NLQS group. Among the areas of tongue, according to the WTCI, the amount of tongue coating in zones A and C was significantly small. In oral microbial analysis, there was no significant difference between the groups at the phylum level. At the genus level, Prevotella, Veillonella, and Streptococcus were predominant in LQS group, whereas Prevotella, Neisseria, and Streptococcus in NLQS group. Conclusions: It was meaningful that insomnia was more likely in LQS group than in NLQS group, and the composition of oral microorganisms was significantly different, which could lead to the diseases caused by stress.

Comparative Study on Maternal Health Status With Island and Land Women in A Middle City Area (중도시내(中都市內) 도서(島嶼)와 육지거주부인(陸地居住婦人)의 모성보건실태비교(母性保健實態比較))

  • Kim, Kyu-Chul
    • Journal of Preventive Medicine and Public Health
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    • v.9 no.1
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    • pp.65-75
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    • 1976
  • To compare the maternal health status between women in island and land, the study was conducted to adjacent land (kukdong) and island (kyonghodong) areas in Yosoo city during April 1976. The results obtained from 174 interviewee in island and 192 in land areas were the same as follow, 1. General characteristics of both area. Age: Land, group 20-29 year of age was the largest. Island, 30-39 year of age was the largest. Education; Low educated group, less than primary grade (land 88.6%, island 93.1%), was predominant in both area. Occupation of herself; agriculture and labor was the main occupation (land 50%, island 82.2%) in both area. Occupation of husband: labor and fishery was general (81.2%) in land, and agriculture and fishery was general (81.6%) in island. Duration of residence; Land, 25.0% being resided here more than 10 years. Island, 64.3% being resided here more than 10 years. 2. Marriage, Pregnancy, and Present children. Average age at marriage; Land, 20.7 years. Island, 20.9 years. Average frequency of pregnancy; Land, 4.4 times. Island, 4.3 times. Wastage of pregnancies: Land, 236 per 1000 pregnancies. Island, 151 per 1000 pregnancies. Wastage occupied by induced abortion; land, 73.5%. island, 60.5%. Number and sex of present children; Land, 3.3 per family, sex ratio 52.4 to 47.6. Island, 3.6 per family, sex ratio 53.3 to 46.7. 3. Prenatal and postnatal care. Prenatal consutation: Land, 16.1% received by doctor or midwife. Island 9.2% received by doctor or midwife. Complications during last pregnancy; Land, 46.6% complained. Island, 51.1% complained. Return to work within 1 week after delivery; Land, 40.6%. Island, 50.6%. 4. Delivery environment Home delivery; Land, 97.4% Island, 97.3%. Delivery attdended by non medical professions at home; Land, 80.2% Island, 93.7%. Solo home delivery; Land, 13.0%, Island, 12.9%. Delivery attended by layman without taking any disinfective preparations; Land, 48.1%, Island, 49.1%. Material mainly used to cut umbilical cord at home; Land, scissors (97.4%). Island, scissora (98.8%). Cord cutting material used without giving any disinfective preparations; Land, 79.9%, Island, 84.0%. Delivery sheets used at home; Cement bag paper (land 50.0%, island, 31.3%). Vinyl sheets (land,17.5%, island, 27.6%). News paper (land, 3.2%. island, 11.7%). No sheets (land 19.5%. island, 12.9%). etc. Maternal Meal; Seaweed soup with rice was the most general in both area. (land 95.3%, island, 91.4%).

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Probing Atomic Structure of Quarternary Aluminosilicate Glasses using Solid-state NMR (다성분계 현무암질 비정질 규산염의 원자 구조에 대한 고상핵자기 공명 분광분석연구)

  • Park, Sun-Young;Lee, Sung-Keun
    • Journal of the Mineralogical Society of Korea
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    • v.22 no.4
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    • pp.343-352
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    • 2009
  • High-resolution Solid-state NMR provides element specific and quantitative information and also resolves, otherwise overlapping atomic configurations in multi-component non-crystalline silicates. Here we report the preliminary results on the effect of composition on the structure of CMAS (CaO-MgO-$Al_2O_3-SiO_2$) silicate glasses, as a model system for basaltic magmas, using the high-resolution 1D and 2D solid-state NMR. The $^{27}Al$ MAS NMR spectra for the CMAS silicate glasses show that four-coordinated Al is predominant, demonstrating that $Al^{3+}$ is network forming cation. The peak position moves toward lower frequency about 4.7 ppm with increasing $X_{MgO}$ due to an increase in $Q^4$(4Si) fraction with increasing Si content, indicating that Al are surrounded only by bridging oxygen. $^{17}O$ MAS NMR spectra for $CaAl_2SiO_6$ and $CaMgSi_2O_6$ glasses qualitatively suggest that NBO fraction in the former is smaller than that in $CaMgSi_2O_6$ glasses. As $^{17}O$ 3QMAS NMR spectrum of model quaternary aluminosilicate glass resolved distinct bridging and non-bridging oxygen environments, atomic structure for natural magmas can also be potentially probed using high-resolution 3QMAS NMR.

Korean Emotion Vocabulary: Extraction and Categorization of Feeling Words (한국어 감정표현단어의 추출과 범주화)

  • Sohn, Sun-Ju;Park, Mi-Sook;Park, Ji-Eun;Sohn, Jin-Hun
    • Science of Emotion and Sensibility
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    • v.15 no.1
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    • pp.105-120
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    • 2012
  • This study aimed to develop a Korean emotion vocabulary list that functions as an important tool in understanding human feelings. In doing so, the focus was on the careful extraction of most widely used feeling words, as well as categorization into groups of emotion(s) in relation to its meaning when used in real life. A total of 12 professionals (including Korean major graduate students) partook in the study. Using the Korean 'word frequency list' developed by Yonsei University and through various sorting processes, the study condensed the original 64,666 emotion words into a finalized 504 words. In the next step, a total of 80 social work students evaluated and classified each word for its meaning and into any of the following categories that seem most appropriate for inclusion: 'happiness', 'sadness', 'fear', 'anger', 'disgust', 'surprise', 'interest', 'boredom', 'pain', 'neutral', and 'other'. Findings showed that, of the 504 feeling words, 426 words expressed a single emotion, whereas 72 words reflected two emotions (i.e., same word indicating two distinct emotions), and 6 words showing three emotions. Of the 426 words that represent a single emotion, 'sadness' was predominant, followed by 'anger' and 'happiness'. Amongst 72 words that showed two emotions were mostly a combination of 'anger' and 'disgust', followed by 'sadness' and 'fear', and 'happiness' and 'interest'. The significance of the study is on the development of a most adaptive list of Korean feeling words that can be meticulously combined with other emotion signals such as facial expression in optimizing emotion recognition research, particularly in the Human-Computer Interface (HCI) area. The identification of feeling words that connote more than one emotion is also noteworthy.

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