• Journal of Applied Biological Chemistry
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• 제49권1호
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• pp.28-32
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• 2006

Effect of organic-C on immobilization and nitrification patterns in acidic soil was examined during 20 weeks incubation period to verify if organic amendments such as composted material can increase soil retention of N by stimulating microbial immobilization of $NH_4^+$. Four treatments were laid out: control without fertilizer N and glucose (treatment code: S), ammonium sulfate (SN), ammonium sulfate with single glucose at the commencement (0 week) of incubation (SNG), and ammonium sulfate with double glucose at 0 and 4 weeks of incubation (SNGG). Glucose application (SNG) significantly increased microbial immobilization of $NH_4^+$ within 1 week of incubation over SN. Immobilization was followed by remineralization thereafter; however, second-application of glucose (SNGG) restored $NH_4^+$ immobilization. At the same time, nitrification was significantly inhibited by glucose application as indicated by consistently low $NO_3^-$ concentration in SNG and SNGG soils, suggesting that microbial assimilation of $NH_4^+$ is predominant compared to nitrification when available C-source is abundant. These results suggest application of chemical fertilizer-N with organic amendment would have beneficial effect on soil-N retention and environmental conservation by reducing production of $NO_3^-$ which is likely to be lost through leaching or denitrification.

• The Plant Pathology Journal
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• 제36권3호
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• pp.244-254
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• 2020

Gom-chwi (Ligularia fischeri) is severely infected with Phytophthora drechsleri, the causal organism of Phytophthora root rot, an economically important crop disease that needs management throughout the cultivation period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizosphere soils collected from various plants and screened for antagonistic activity against P. drechsleri. A total of 172 bacterial strains were isolated, of which, 49 strains showed antagonistic activities by dual culture assay. In the seedling assay, six out of the 49 strains showed a predominant effect on suppressing P. drechsleri. Among the six strains, the ObRS-5 strain showed remarkable against P. drechsleri when treated with seed dipping or soil drenching. The ObRS-5 strain was identified as Enterobacter asburiae based on 16S ribosomal RNA gene sequences analysis. The bacterial cells of E. asburiae ObRS-5 significantly suppressed sporangium formation and zoospore germination in P. drechsleri by 87.4% and 66.7%, respectively. In addition, culture filtrate of E. asburiae ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacterial cells, filtrate, and culture solution of E. asburiae ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, E. asburiae ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi.

• 한국해양학회지
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• 제30권2호
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• pp.138-146
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• 1995

본 연구는 강화도 연안 새우류(Decapoda, Macrura) 군집의 우점종인 돛대 기새우 (Leptochela gracilis (STIMPSON, 1860))의 성장 양상을 조사하기 위해 실시되었다. 시료는 1개의 동일한 정점에서 낭장망을 이용하여 채집하였으며 1993년 4월부터 1994 년 1월까지 8월을 제외한 월별 채집을 하였다. 돛대 기새우의 포란 개체는 4월에서 9 월까지 출현하였는데 5월에서 7월의 포란률이 70% 이상나타나 포란성기로 여겨진다. 또한 성비는 4월을 제외한 조사 전월에 걸쳐 암컷이 우세하였으며 포란기와 비포란기 사이에 유의한 차이를 보이지 않았다(p>0.05). 돛대 기새우 개체군은 장기세대와 단기 세대의 두 형태의 세대로 나눌 수 있었고 장기세대의 수명은 1년에서 1년 3개월 정도 로 추측된다. 단기세대의 경우는 9월 포란군에 의해 산란되어 12월이후 감소하는데, 이들이 환경 요인들의 악화로 사망하는 것인지, 월동이후 성장률의 차이에 의해 장기 세대 병합되는 것인지는 밝힐 수 없었다. 장기세대를 Von Bertalanffy 성장식과 계절 변동을 고려한 Pauly and Gaschutz 성장식에 적용시켜본 결과 Pauly and Gaschutz 성 장식이 더 적합한 것으로 나타났다.

• 지구물리와물리탐사
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• 제13권2호
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• pp.144-152
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• 2010

제주도 화산암 두께변화를 연구하기 위하여, 단주기와 광대역 지진계를 각각 8개와 4개 조사지 지표에 설치하여 상시미동 자료를 30 ~ 60분 동안 100 Hz 샘플률로 기록하였다. 인공잡음은 주간자료의 1.8 Hz 이상에서 나타나는 반면, 자연잡음은 0.4 ~ 0.8 Hz 범위에서 주간 및 야간 기록자료 모두에 기록되어 나타난다. H/V 스펙트럼비를 이용하여 구한 우세주파수는 0.2 ~ 0.7 Hz 범위에 분포한다. 우세주파수는 한라산 중심부로 갈수록 점진적으로 작은 값을 보여, 기존의 중력 및 자력모델의 결과처럼 제주도의 기반암이 섬 중심부 아래로 휜 구조임을 반영한다. 기반암 상부 현무암의 평균 횡파속도를 1,800 m/s로 가정할 경우, 각 조사지의 기반암 깊이는 약 640 ~ 2,140 m 정도로 평가된다.

• Geomechanics and Engineering
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• 제25권2호
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• pp.143-157
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• 2021

Expansive soil is the most predominant geologic hazard which shows a large amount of shrinkage and swelling with changes in their moisture content. This study investigates the macro-mechanical and micro-structural behaviours of dredged natural expansive clay from coal mining treated with ordinary Portland cement or hydrated lime addition. The stabilised expansive soil aims for possible reuse as pavement materials. Mechanical testing determined geotechnical engineering properties, including free swelling potential, California bearing ratio, unconfined compressive strength, resilient modulus, and shear wave velocity. The microstructures of treated soils are observed by scanning electron microscopy, x-ray diffraction, and energy dispersive spectroscopy to understand the behaviour of the expansive clay blended with cement and lime. Test results confirmed that cement and lime are effective agents for improving the swelling behaviour and other engineering properties of natural expansive clay. In general, chemical treatments reduce the swelling and increase the strength and modulus of expansive clay, subjected to chemical content and curing time. Scanning electron microscopy analysis can observe the increase in formation of particle clusters with curing period, and x-ray diffraction patterns display hydration and pozzolanic products from chemical particles. The correlations of mechanical properties and microstructures for chemical stabilised expansive clay are recommended.

• Environmental Engineering Research
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• 제24권1호
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• pp.159-172
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• 2019

To investigate the influence of pollution events on the chemical composition and formation processes of aerosol particles, 24-h integrated size-segregated particulate matter (PM) was collected during the fall season at an urban site of Gwangju, Korea and was used to determine the concentrations of mass, water-soluble organic carbon (WSOC) and ionic species. Furthermore, black carbon (BC) concentrations were observed with an aethalometer. The entire sampling period was classified into four periods, i.e., typical, pollution event I, pollution event II, and an Asian dust event. Stable meteorological conditions (e.g., low wind speed, high surface pressure, and high relative humidity) observed during the two pollution events led to accumulation of aerosol particles and increased formation of secondary organic and inorganic aerosol species, thus causing $PM_{2.5}$ increase. Furthermore, these stable conditions resulted in the predominant condensation or droplet mode size distributions of PM, WSOC, $NO_3{^-}$, and $SO{_4}^{2-}$. However, difference in the accumulation mode size distributions of secondary water-soluble species between pollution events I and II could be attributed to the difference in transport pathways of air masses from high-pollution regions and the formation processes for the secondary chemical species. The average absorption ${\AA}ngstr{\ddot{o}}m$ exponent ($AAE_{370-950}$) for 370-950 nm wavelengths > 1.0 indicates that the BC particles from traffic emissions were likely mixed with light absorbing brown carbon (BrC) from biomass burning (BB) emissions. It was found that light absorption by BrC in the near UV range was affected by both secondary organic aerosol and BB emissions. Overall, the pollution events observed during fall at the study site can be due to the synergy of unfavorable meteorological conditions, enhanced secondary formation, local emissions, and long-range transportation of air masses from upwind polluted areas.

• 한국환경과학회지
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• 제28권1호
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• pp.107-123
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• 2019

The aerosol chemical components in $PM_{2.5}$ in several regions (Seoul, Busan, Daejeon, and Jeju Island) were investigated with regard to their concentration characteristics and optical properties. The optical properties of the various aerosol components (e.g., water-soluble, insoluble, Black Carbon (BC), and sea-salt) were estimated using hourly and daily aerosol sampling data from the study area via a modeling approach. Overall, the water-soluble component was predominant over all other components in terms of concentration and impact on optical properties (except for the absorption coefficient of BC). The annual mean concentration and Aerosol Optical Ddepth (AOD) of the water-soluble component were highest in Seoul (at the Gwangjin site) ($26{\mu}g/m^3$ and 0.29 in 2013, respectively). Further, despite relatively moderate BC concentrations, the annual mean absorption coefficient of BC ($21.7Mm^{-1}$) was highest in Busan (at the Yeonsan site) in 2013, due to the strong light absorbing ability of BC. In addition, high AODs for the water-soluble component were observed most frequently in spring and/or winter at most of the study sites, while low values were noted in summer and/or early fall. The diurnal variation in the AOD of each component in Seoul (at the Gwangjin site) was slightly high in the morning and low in the afternoon during the study period; however, such distinctions were not apparent in Jeju Island (at the Aweol site), except for a slightly high AOD of the water-soluble component in the morning (08:00 LST). The monthly and diurnal differences in the AOD values for each component could be attributed to the differences in their mass concentrations and Relative Humidities (RH). In a sensitivity test, the AODs estimated under RH conditions of 80 and 90% were factors of 1.2 and 1.7 higher, respectively, than the values estimated using the observed RH.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.82-91
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• 2019

Objective: This experiment was conducted to compare the structure and composition of ruminal microorganisms in goats with high and low neutral detergent fibre (NDF) digestibility. Methods: Nineteen crossbred goats were used as experimental animals and fed the same total mixed rations during the 30-day pre-treatment and 6-day digestion trialperiods. All faeces were collected during the digestion period for measuring the NDF digestibility. Then, high and the low NDF digestibility individuals were chosen for the high NDF digestibility group (HFD) and low NDF digestibility group (LFD), respectively. Rumen contents were collected for total microbial DNA extraction. The V4 region of the bacterial 16S rRNA gene was amplified using universal primers of bacteria and sequenced using high-throughput sequencer. The sequences were mainly analysed by QIIME 1.8.0. Results: A total of 18,694 operational taxonomic units were obtained, within 81.98% belonged to bacteria, 6.64% belonged to archaea and 11.38% was unassigned microorganisms. Bacteroidetes, Firmicutes, and Proteobacteria were the predominant microbial phyla in both groups. At the genus level, the relative abundance of fifteen microorganisms were significantly higher (p<0.05) and six microorganisms were extremely significantly higher (p<0.01) in LFD than HFD. Overall, 176 core shared genera were identified in the two groups. The relative abundance of 2 phyla, 5 classes, 10 orders, 13 families and 15 genera had a negative correlation with NDF digestibility, but only the relative abundance of Pyramidobacter had a positive correlation with NDF digestibility. Conclusion: There were substantial differences in NDF digestibility among the individual goats, and the NDF digestibility had significant correlation with the relative abundance of some ruminal microorganisms.

• Earthquakes and Structures
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• 제16권5호
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• pp.563-573
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• 2019

When generating spectrum-compatible artificial ground motion in engineering practices, the effect of the variation in fitting parameters on the distribution of the peak ground displacement (PGD) has not yet drawn enough attention. In this study, a method for simulating ground motion matching for multiple targets is developed. In this method, a frequency-dependent amplitude envelope function with statistical parameters is introduced to simulate the nonstationarity of the frequency in earthquake ground motion. Then, several groups of time-history acceleration with different temporal and spectral nonstationarities were generated to analyze the effect of nonstationary parameter variations on the distribution of PGD. The following conclusions are drawn from the results: (1) In the simulation of spectrum-compatible artificial ground motion, if the acceleration time-history is generated with random initial phases, the corresponding PGD distribution is quite discrete and an uncertain number of PGD values lower than the limit value are observed. Nevertheless, the mean values of PGD always meet the requirement in every group. (2) If the nonstationary frequencies of the ground motion are taken into account when fitting the target spectrum, the corresponding PGD values will increase. A correlation analysis shows that the change in the mean and the dispersion values, from before the frequencies are controlled to after, correlates with the modal parameters of the predominant frequencies. (3) Extending the maximum period of the target spectrum will increase the corresponding PGD value and, simultaneously, decrease the PGD dispersion. Finally, in order to control the PGD effectively, the ground motion simulation method suggested in this study was revised to target a specified PGD. This novel method can generate ground motion that satisfies not only the required precision of the target spectrum, peak ground acceleration (PGA), and nonstationarity characteristics of the ground motion but also meets the required limit of the PGD, improving engineering practices.

• 한국축산식품학회지
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• 제43권3호
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• pp.471-490
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• 2023

In this study, the duck eggs were salted with none or 2.5% and 5.0% (v/v) of liquid smoke (LS), respectively. As a control, samples salted without LS were used. The 2-thiobarbituric acid (TBA) values, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, and reducing power of the three groups were tested at 0, 7, 14, and 21 and 28 days to determine the effects of LS on the antioxidant activity of treated eggs. In addition, gas chromatography-mass spectrometry (GC-MS) and electronic nose (E-Nose) were used to analyze the volatile flavor components of fresh duck eggs, LS, control, and salted duck eggs enriched with 2.5% (v/v) LS after 28 days of salting. The TBA value considerably increased with an increase in salting period, and the treated egg's TBA value significantly associated with LS concentration. The TBA value decreased as the LS concentration increased. The amount of LS present was highly associated with their capacity to scavenge DPPH radicals. The reducing power of the samples was substantially correlated with the LS concentration, and the reducing power increased with increasing LS concentration. The GC-MS data revealed that phenols and ketones were the predominant chemicals present in the LS, and they were also found in the eggs added to the LS even though they were absent in the fresh eggs and control. The flavor of the control group and treated eggs with LS differed significantly, according to the principal component analysis and radar map of the E-nose. The texture study results revealed that the LS significantly impacted the hardness, cohesiveness, and chewiness of eggs.