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A Study on the Konjak Mannan-hydrolyzing Enzymes from Aspergillus awamori (Aspergillus awamori 가 생산하는 konjak mannan 분해효소에 관한 연구)

  • Chang, Kyung-Jung;Lee, Su-Rae
    • Applied Biological Chemistry
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    • v.15 no.3
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    • pp.199-206
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    • 1972
  • As a study on the konjak mannan-hydrolyzing enzymes from Aspergillus awamori, the culture conditions for enzyme formation, purification and properties of the enzymes and the effect of gamma-irradiation on the enzymatic hydrolysis were investigated. The results are summarized as follows: 1) A strain of A. awamori was selected as having the highest productivity of mannanase among 81 species of molds. 2) The optimum conditions for solid culture on wheat bran were 3 days of culture period, pH 4 of spraying water and 100% addition of tap water. 3) The optimum conditions for shaking culture were 6 days of culture period, addition of 0.1% xylose plus 0.5% konjak mannan and of 0.04% peptone. 4) Konjak mannan-hydrolyzing enzymes were separated into fraction I and fraction II by ammonium sulfate fractionation and DEAE-Sephadex column chromatography. 5) Fractions I and II showed pH optima of 4, pH stability of $3.5{\sim}5$ and $3{\sim}6$ and the extent of hydrolyzing konjak mannan 9% and 50%, respectively. 6) Hydrolysis of konjak mannan by a crude enzyme preparation was partially accerelated by gamma-irradiation of substrate above 0.5 Mrad and the effect was more remarkable by irradiating in wet state than in dry state. 7) Gamma-irradiation of konjak mannan brought about the increase in reducing power and decrease in viscosity and the effect was more remarkable in wet state than in dry state.

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Analysis on the behavior of shield TBM cable tunnel: The effect of the distance of backfill grout injection from the end of skin plate (뒷채움 주입 거리에 따른 전력구 쉴드 TBM 터널의 거동 특성 분석)

  • Cho, Won-Sub;Song, Ki-Il;Ryu, Hee-Hwan
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.16 no.2
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    • pp.213-224
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    • 2014
  • Recently, tunnelling with TBM is getting popular for the construction of cable tunnel in urban area. Mechanized tunnelling method using shield TBM has various advantages such as minimization of ground settlement and prevention of vibration induced by blasting that should be accompanied by conventional tunnelling. In Korea, earth pressure balance (EPB) type shield TBM has been mainly used. Despite the popularity of EPB shield TBM for cable tunnel construction, study on the mechanical behavior of cable tunnel driven by shield TBM is insufficient. Especially, the effect of backfill grout injection on the behavior of cable tunnel driven by shield TBM is investigated in this study. Tunnelling with shield TBM is simulated using 3D FEM. The distance of backfill grout injection from the end of shield skin varies. Sectional forces such as axial force, shear force and bending moment are monitored. Vertical displacement at the ground surface is measured. Futhermore, the relation between volume loss and the distance of backfill grout injection from the end of skin plate is derived. Based on the stability analysis with the results obtained from the numerical analysis, the most appropriate injection distance can be obtained.

Antibacterial Effect of Gelatin/Ag Nanoparticle Biocomposite Prepared Using Solution Plasma Generated by Unipolar DC Power (단극성 직류전원으로 유도된 용액 플라즈마를 이용하여 제조한 젤라틴/은 나노입자 생체복합재료의 항균 효과)

  • Kim, Seong-Cheol;Yoon, Gook-Jin;Nam, Sang-Woo;Lee, Sang-Yul;Kim, Jung-Wan
    • Microbiology and Biotechnology Letters
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    • v.40 no.4
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    • pp.403-408
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    • 2012
  • Gelatin/Ag nanoparticle (AgNP) biocomposite was synthesized using the solution plasma process (SPP) that has been recently introduced as an effective method for synthesis of nanoparticles. In this study, gelatin/AgNP biocomposite was synthesized using various concentrations of Ag precursor ($AgNO_3$) and gelatin in the range of 1-5 mM and 1-3% (w/w), respectively, without using any chemical reducing agent. Physical properties of the gelatin/AgNP biocomposites were analyzed using EDS, FE-SEM, and TEM. The results indicated that spherical AgNPs with approximately 12~20 nm in diameter were synthesized successfully in the gelatin matrix by SPP. As the concentration of gelatin was increased (3%, w/w), disperse stability of AgNP was improved and micro-pores of gelatin became smaller and denser in the 3D scaffold. Bactericidal activity of the AgNPs was examined against Staphylococcus aureus and Escherichia coli by measuring zone of growth inhibition and decrease in colony forming unit (CFU). CFUs of S. aureus and E. coli were decreased approximately to 56% and 0%, respectively, by the gelatin/AgNP biocomposite, Ag5G3.

Temporary Metadata Journaling Scheme to Improve Performance and Stability of a FAT Compatible File System (FAT 파일 시스템의 호환성을 유지하며 성능과 안정성을 향상시키는 메타데이터 저널링 기법의 설계)

  • Hyun, Choul-Seung;Choi, Jong-Moo;Lee, Dong-Hee;Noh, Sam-H.
    • Journal of KIISE:Computer Systems and Theory
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    • v.36 no.3
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    • pp.191-198
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    • 2009
  • The FAT (File Allocation Table) compatible file system has been widely used in mobile devices and memory cards because of its data exchangeability among numerous platforms recognizing the FAT file system. By the way. modern embedded systems have tough demands for instant power failure recovery and superior performance for multimedia applications. The key issue is how to achieve the goals of superior write performance and instant booting capability while controlling compatibility issues. To achieve the goals while controlling compatibility issues. we devised a temporary meta-data journaling scheme for a FAT compatible file system. Benchmark results of the scheme implemented in a FAT compatible file system shows that it really improves write performance of the FAT file system by converting small random write for meta-data update to a large sequential write in journaling area. Also, it provides natural way to implement the instant booting capability. Nevertheless, the file system compatibility is temporarily compromised by the scheme because it stores updated meta-data in the temporary journaling area rather than to their original locations. However, the compatibility can be fully recovered at any time by journal-flushing that copies meta-data in journaling area to their original locations. Generally, the journal-flushing is done before un-mounting a memory card so that it can be used in other mobile devices which recognized FAT file system but not the temporary meta-data journaling scheme.

Development of the Wide Passenger Door System of EMU based on the High Precision Stop Performance (정위치 정차 성능 기반 전동차 광폭 출입문 시스템 개발 연구)

  • Kim, Moosun;Hong, Jae-Sung;Kim, Jungtai;Jang, Dong Uk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.1
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    • pp.618-624
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    • 2017
  • In Seoul and most metropolitan cities, urban trains are delayed due to high congestion during commute times. The delay effect of passengers boarding and disembarking is also significant. In this study, a wide passenger door system was developed as a way to improve the scheduled speed of urban trains by decreasing the passengers' flow time. The door size was defined experimentally to shorten the entrance time. The optimum door size was also determined to improve the stop precision performance of the train while considering the interference effect with peripheral devices. Because the change in door size changes the structural characteristics of the vehicle, the structural stability of a train was analyzed numerically. A prototype of the wide door system was made, and the proposed design was verified using functional and endurance tests. The systematic development process can be used as design data for door size definition and system production when applying a wide door to improve the scheduled speed.

Development of an Integrated Forecasting and Warning System for Abrupt Natural Disaster using rainfall prediction data and Ubiquitous Sensor Network(USN) (농촌지역 돌발재해 피해 경감을 위한 USN기반 통합예경보시스템 (ANSIM)의 개발)

  • Bae, Seung-Jong;Bae, Won-Gil;Bae, Yeon-Joung;Kim, Seong-Pil;Kim, Soo-Jin;Seo, Il-Hwan;Seo, Seung-Won
    • Journal of Korean Society of Rural Planning
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    • v.21 no.3
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    • pp.171-179
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    • 2015
  • The objectives of this research have been focussed on 1) developing prediction techniques for the flash flood and landslide based on rainfall prediction data in agricultural area and 2) developing an integrated forecasting system for the abrupt disasters using USN based real-time disaster sensing techniques. This study contains following steps to achieve the objective; 1) selecting rainfall prediction data, 2) constructing prediction techniques for flash flood and landslide, 3) developing USN and communication network protocol for detecting the abrupt disaster suitable for rural area, & 4) developing mobile application and SMS based early warning service system for local resident and tourist. Local prediction model (LDAPS, UM1.5km) supported by Korean meteorological administration was used for the rainfall prediction by considering spatial and temporal resolution. NRCS TR-20 and infinite slope stability analysis model were used to predict flash flood and landslide. There are limitations in terms of communication distance and cost using Zigbee and CDMA which have been used for existing disaster sensors. Rural suitable sensor-network module for water level and tilting gauge and gateway based on proprietary RF network were developed by consideration of low-cost, low-power, and long-distance for communication suitable for rural condition. SMS & mobile application forecasting & alarming system for local resident and tourist was set up for minimizing damage on the critical regions for abrupt disaster. The developed H/W & S/W for integrated abrupt disaster forecasting & alarming system was verified by field application.

Seismic and Stress Analysis of 72.5kV GIS for Technical Specification of KEPCO (72.5kV GIS 전력 장비의 KEPCO 기준 내진 및 응력 해석)

  • Lee, Jae-Hwan;Kim, Young-Joong;Kim, So-Ul;Bang, Myung-Suk
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.30 no.3
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    • pp.207-214
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    • 2017
  • High voltage electric power transmitter GIS(Gas Insulated Switchgear) above 72.5kV needs to satisfy domestic Korean peninsular standard(ES-6110-0002) in KEPCO with respect to normal and special operation conditions which include internal gas pressure, dead weight, wind and seismic load. Some other requirements not described in Korean standard can be applied from other international standards such as IEC(International Electronical Committee) 62271-203 and 62271-207. The GIS is a kind of pressure vessel structure made of aluminum and filled with SF6 gas of internal pressure 0.4~0.5MPa. Finite element analysis of GIS is performed with such operational loads including seismic loading and the stability and reliability is determined according to ASME BPVC(Boiler and Pressure Vessel Code) SEC. VIII standard where the allowable stress level of the pressure vessel is suggested. The result shows that the stress of GIS is satisfied the allowable stress level and the safety factor is about 2.3 for Korean peninsular standard.

A Study on the Measurement and Effect Factors of Productivity of the Korean Ocean Carriers (금융위기 이후 국적 외항선사의 생산성 측정과 영향요인에 관한 연구)

  • Nam, Hyung-Sik;Ahn, Ki-Myung
    • Journal of Navigation and Port Research
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    • v.44 no.4
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    • pp.338-346
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    • 2020
  • In this study, we first measured the Malmquist productivity index by DEA among the Korean ocean carriers. Second, it was intended to present measures to improve productivity by identifying the influence and discriminating power between productivity and the major financial ratios (profitability, financial stability, liquidity, efficiency and value-added productivity). Compared to 2017, there are 11 more shipping carriers with decline in productivity (MPI) than those with an increase in 2018. The increase in productivity is attributed to an increase in the Technology Change Index (TCI) affected by the external environment. There is strong significant correlation between the productivity (MPI) and the management efficiency (CRS). Additionally, the TECI (TECHI) index of the technological efficiency changes from internal factors of the shipping carrier is significantly higher than that of the efficient shipping carrier. This is because of the differences in scale efficiency. The ratio of charter cost/sales is also lower than that of the carriers with high productivity (0.17) and with low productivity (0.21). With 7% of the shipping carrier with MPI>1, only 1% of MPI <1 is found to have a significant impact on its productivity.

Novel Intensity-Based Fiber Optic Vibration Sensor Using Mass-Spring Structure (질량-스프링 구조를 이용한 새로운 광세기 기반 광섬유 진동센서)

  • Yi, Hao;Kim, Hyeon-Ho;Choi, Sang-Jin;Pan, Jae-Kyung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.6
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    • pp.78-86
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    • 2014
  • In this paper, a novel intensity-based fiber optic vibration sensor using a mass-spring structure, which consists of four serpentine flexure springs and a rectangular aperture within a proof mass, is proposed and its feasibility test is given by the simulation and experiment. An optical collimator is used to broaden the beam which is modulated by the displacement of the rectangular aperture within the proof mass. The proposed fiber optic vibration sensor has been analyzed and designed in terms of the optical and mechanical parts. A mechanical structure has been designed using theoretical analysis, mathematical modeling, and 3D FEM (Finite Element Method) simulation. The relative aperture displacement according to the base vibration is given using FEM simulation, while the output beam power according to the relative displacement is measured by experiment. The simulated sensor sensitivity of $15.731{\mu}W/G$ and detection range of ${\pm}6.087G$ are given. By using reference signal, the output signal with 0.75% relative error shows a good stability. The proposed vibration sensor structure has the advantages of a simple structure, low cost, and multi-point sensing characteristic. It also has the potential to be made by MEMS (Micro-Electro-Mechanical System) technology.

Characteristics of Rice Flours Prepared by Moisture-Heat Treatment (수분-열처리에 의해 제조한 멥쌀가루의 특성)

  • Lee Mi-Kyung;Shin Mal-Shick
    • Korean journal of food and cookery science
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    • v.22 no.2 s.92
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    • pp.147-157
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    • 2006
  • To improve the properties of non-waxy rice flours for baking, soaked-wet milled rice flour (SWRF) was adjusted moisture content (MC) to 30 and 50 % and heated at 50 and $70^{\circ}C$ in a shaking water bath for 6 and 18 hrs, respectively. Moisture-heat treated rice flours were investigated with regard to particle size distribution, and morphological, physicochemical and pasting properties for comparing dry and wet milled flours. The particle size of rice flour treated with 30% MC was distributed between $4-20{\mu}m\;and\;100-200{\mu}m$, like SWRF. The particles above $200{\mu}m$ in the flour were produced at higher heating temperature. By SEM, starch granules were found in the rice flours treated with 30% MC, who whereas aggregated starch granules were shown in the flours treated with 50% MC. Moisture-heat treatment using higher MC and heating temperature decreased the lightness and increased the yellowness of non-waxy rice flours. Water binding capacity of 30% moisture treated rice flour was similar to that of SWRF. In the same moisture treated rice flour, swelling power was higher, but solubility was lower at $50^{\circ}C$ than at $70^{\circ}C$. The initial pasting temperature by RVA increased after moisture-heat treatment. The peak viscosity of moisture-heat treated rice flour was higher for 30% moisture than that of the others. The rice flour treated with 30% MC and heated at $50^{\circ}C$ showed low setback and increased stability for retrogradation.