• Fisheries and Aquatic Sciences
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• 제19권1호
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• pp.1.1-1.6
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• 2016

Although soft tunic syndrome (STS) in the ascidian is a serious disease, helpful measures have yet not been established. It was examined in this study by applying aniti-parasitic drugs to eradicate the causative protozoa Azumiobodo hoyamushi from infected ascidians. Formalin was synergistic in killing parasites in vitro when co-treated with hydrogen peroxide ($H_2O_2$) or bronopol, but not with chloramine-T or povidone-iodine (PVP-I), when tested with in vitro parasite culture. The synergistic effects did not change when $formalin-H_2O_2$ (or bronopol) ratios were changed. It was found that treatment periods less than 60 min achieved a sub-maximal efficacy. Increasing drug concentration while keeping 30 min period improved anti-parasitic effects. Anti-parasitic effects of $formalin(F)+H_2O_2$(H) were also assessed in an in vivo STS model infected with cultured parasites. It was observed that combined 50 (40F + 10H) and 100 (80F +20H) ppm were effective in partially preventing STS-caused mortality. In horizontally transmitted artificial STS model, significant prevention of ascidian mortality was also observed after 50 ppm. Marked reduction of living parasites were noted after drug treatments in vivo. The results provide a highly useful basis to develop a preventive or treatment measure against the currently uncontrollable STS in the ascidian.

• Journal of Chest Surgery
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• 제54권2호
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• pp.137-142
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• 2021

Background: Refractory empyemas with collapsed lung and persistent bronchopleural fistulas pose significant problems to thoracic surgeons and impose a substantial burden in terms of morbidity and mortality. The modified Eloesser flap procedure is a useful palliative option for clearing infections. Herein, we present our experiences with the modified Eloesser flap procedure in mixed suppurative lung pathologies with a new technique of irrigation for persistent infection. Methods: A retrospective review was carried out of 56 patients who underwent the modified Eloesser flap with continuous irrigation at Katurba Medical College. These patients had severe morbidities and were not suitable for major thoracic resection surgery, and electively underwent modified Eloesser flap surgery. Regular follow-up was done at 1, 3, 6, and 12 months. Patients with persistent infections were treated with our continuous irrigation technique. Results: The most important finding was that all patients with active sputum acid-fast bacilli-positive findings became sputum smear-negative during the first month of follow-up. Half (50%) of the patients had a patent stoma. Eleven patients had persistent infections, necessitating continuous irrigation. The infection was fully cleared after 1 month in 9 patients, while 2 patients required second irrigation and continued to receive follow-up. In the remaining 50% of the patients, the stoma closed completely, and the lung expanded fully. Conclusion: The modified Eloesser flap is a simple procedure. In suppurative pathologies, infections were well controlled and the general condition of the patients improved. Our continuous irrigation method showed promising results in patients with persistent purulent discharge.

• Parasites, Hosts and Diseases
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• 제54권5호
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• pp.667-671
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• 2016

Myiasis is a relatively common infection of animals kept as pets, although only 1 case of canine myiasis has been described so far in the Republic of Korea. In the present study, we report an additional case of canine wound myiasis with identification of its causative agent, Lucilia sericata. An 8-year-old male Siberian husky dog was referred with anorexia, vomiting, and diarrhea to the Chungbuk National University Veterinary Medical Center, Cheongju-si (city), Chungcheongbuk-do (province), Korea in July 2013. Physical examination indicated the patient had a deep wound filled with a maggot swarm as a left gluteal lesion. A total of 216 maggots were removed by forceps, and the wounded area was sponged with gauzes and disinfected with 70% alcohol and a povidone-iodine solution. After daily care and suturing the wound, the patient was discharged at day 19 after admission. Recovered worms possessed morphological characteristics similar to those of L. sericata, namely, a sub-cylindrical body with 6-8 lobed anterior spiracles, round shaped with a button surrounded by a peritremal ring with no gaps, and similar distances between dorsal, median, and outer papillae of the 12th segment. Additionally, cox1 partial sequences (528 bp) obtained in the present study showed 100% identity with those of L. sericata (GenBank no. KT272854.1). L. sericata is indicated as a pathogen of myiasis infection not only in humans, but also in animals kept as pets in Korea.

• Pediatric Infection and Vaccine
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• 제25권1호
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• pp.17-25
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• 2018

목적: 국내에서 소아를 대상으로 시행하는 혈액배양의 적응증과 방법, 정도 관리 현황을 파악하고자 하였다. 방법: 종합병원에 근무하는 소아감염분과 전문의(소아감염)와 신생아집중치료센터를 운영하는 병원에 근무하는 신생아분과 전문의(신생아)를 대상으로 온라인 설문조사를 하였다. 결과: 소아감염의 81.1% (30/37)와 신생아의 72.2% (52/72)가 설문에 응하였다. 적응증과 무관하게 정규 검사로 혈액배양을 실시하는 경우는 소아감염의 33.3%, 신생아의 59.6%였다. 소아감염의 40%와 신생아의 65.4%가 균혈증 의심 환자에서 혈액배양을 1회 시행한다고 했다. 가장 많이 사용되는 피부 소독제는 포비돈-아이오딘이었고, 여러 피부 소독제를 사용하는 경우 그 순서는 기관마다 다양하였다. 배양 양성률 및 오염률을 모니터링하는 기관은 전체의 2/3 정도였으나 결과를 채혈자에게 피드백하는 곳은 적었다. 채혈 담당자에게 정기적인 교육을 하는 곳은 전체의 1/2 이하였다. 결론: 기관별로 혈액배양의 적응증과 방법이 다양하였으며 정도 관리를 적극적으로 시행하는 병원은 적었다. 소아에서 실시되는 혈액배양의 질 향상을 위해 표준 지침의 정립이 필요하며 정도 관리를 위한 기관들의 노력이 요구된다.