• Korean Journal of Poultry Science
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• v.36 no.4
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• pp.337-342
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• 2009

This work was conducted to investigate the manure excreta and chemical compositions of layers. Two hundred 1-d-old ISA Brown layers were used in this work, and ten of 200 layers were selected for measurement of manure excreta with feeding phase. Means of the manure excreta per bird, the moisture content of poultry, and the dried manure were $124.7\;{\pm}\;27.5\;g$/bird/day, $76.9\;{\pm}\;1.9%$, and $29.1\;{\pm}\;3.9\;g$/bird/day, respectively. N, $P_2O_5$, and $K_2O$ of manure (55 weeks) were $1.39\;{\pm}\;0.139$, $0.62\;{\pm}\;0.11$ and $0.68\;{\pm}\;0.09%$, respectively. pH, $BOD_5$, $COD_{Mn}$, and suspended solids (SS) were $8.19\;{\pm}\;0.71$, $50,266\;{\pm}\;621\;mg/L$, $62,832\;{\pm}\;803\;mg/L$, and $121,725\;{\pm}\;16,165\;mg/L$, respectively. Heavy metal contents in the manure were $0.003\;{\pm}\;0.0013\;ppm$ for Hg and $0.008\;{\pm}\;0.0012\;ppm$ for Pb, the latter of which was highly low compared with the standard (150 ppm), but no Cd was detected. The results of this work show the average excretion amounts and chemical compositions of layer's manure, which is used as an organic fertilizer.

• Journal of Microbiology and Biotechnology
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• v.21 no.1
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• pp.62-70
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• 2011

The antibacterial effects of nine lactic acid bacteria (LAB) against Campylobacter jejuni were investigated by using agar gel diffusion and co-culture assays. Some differences were recorded between the inhibition effects measured with these two methods. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, exhibited a clear anti- Campylobacter activity in co-culture assay with dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the supplementation of such medium with a cellulase A complex (Beldem S.A.) enhanced the antimicrobial effect of both LAB strains. The co-culture medium acidification and the C. jejuni were positively correlated with the cellulase A concentration. The antibacterial effect was characterized by the lactic acid production from the homofermentative E. faecium THT and the lactic and acetic acids production from the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties of LAB strains and enzyme combination could be used in strategies aiming at the reduction of Campylobacter prevalence in the poultry production chain and consequently the risk of human infection.

• Korean Journal of Poultry Science
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• v.21 no.3
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• pp.195-205
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• 1994

Effects of the addition of \beta-glucanase to barley-based layer diets were examined by feeding 200 Leghorn layers with corn-based (Control) and \beta-glucanase supplemented diets (Barley+ Enzyme). The results obtained are sumrrarized as follows. 1. There were no siginificant (P>0.05) differences in hen-day egg production(%) and average egg weight between two treatments, indicating that the \beta-glucanase supplemented barley could successfully replace the commonly used corn in the layer diets. 2. Although there was no statistical difference (P>0.05) between two treatments, the daily feed consumption was numerically high in layers fed the barly diet compared to the corn-based diet. 3. Availabilities of crude fat and crude fiber of the barley diet were significantly poor (P<0.05) as compared to corn diet. 4. The \beta-glucarase supplementation depressed the viscosity of barley diets and excreta from therm. 5. Both serum and egg yolk cholesterol were not significantly affected by the addition of \beta-glucarase in the barley based diet. Our data indicate that the barley grain supplemented with \beta-glucarase can be sucessfully used as an energy source of layer diet when there is a price advantage. Although some possibilities to produce low cholesterol egg were recognized in this study, further studies pertaining to long-term feeding experiment and elucidaton of the metabolic interrelationship between serum and yolk cholesterol, are required to confirm the result.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1487-1494
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• 2003

Studies were conducted with intact White Leghorn roosters to determine the true metabolisable energy (TME) and the true amino acid availability (TAAA) in five dehulled and hulled soybean meals produced in China. 60 roosters, kept in individual cages, were fasted for 48 h and then tube-fed 50 g of one of experimental feedstuffs and their excreta was then collected for the subsequent 48 h period. Two separate collection periods were used with each meal being fed to 12 roosters. The birds were given a 15-day recovery period between collection periods. An additional 12 roosters were either fasted or fed a protein-free diet in order to estimate the extent of endogenous losses of energy and amino acids in excreta. The average values per bird for nitrogen loss, endogenous energy losses (EEL) and endogenous energy losses corrected to zero-nitrogen balance ($EEL_n$) were found to be 0.74 g, 47.0 kJ and 21.5 kJ, respectively. It was found that the TME and TAAA values of dehulled soybean meal were higher than those of hulled soybean meal. The TME and nitrogen-corrected TME metabolisable energy values of dehulled soybean meal were 10.58 and 10.74 MJ/kg, respectively, while the corresponding values for hulled soybean meal were 10.03 and 10.27 MJ/kg, respectively. The average indispensable and dispensable amino acid availability of dehulled soybean meal was 92.1 and 93.5%, compared with 89.3 and 91.4% for hulled soybean meal. Dehulled soybean meal would therefore appear to be superior to hulled soybean meal as a source of protein and energy for use in poultry rations.

• Journal of Animal Environmental Science
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• v.6 no.3
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• pp.141-151
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• 2000

Over-grown seaweeds in Donghae(east sea of Korea peninsula) may impedes ocean environment, however, they can be a good nutrient resources for poultry feeds if they are utilized properly. In this study, seaweeds powder were tested for laying hens\` ration to investigate the effects on egg production rate, egg quality and calcium phosphorus excretion. One hundred 65wks-old brown layers were fed for 5 weeks alotted with seaweeds powder addition to experimental diet by 0(control), 0.5, 1.0 and 2.0%, respectively, and obtained following results; 1. Seaweeds proved a mid-protein low-energy feed resources with planty of K, Na, Ca, Mg, Sr and Fe. 2. Seaweeds addition by 0.5% and 1.0% improved egg production rate and egg-mass output markedly(p<0.05) than control. Seaweeds addition did not alter cholesterol level of yolk and yolk index, however egg shell thickness showed increasing trend by increasing seaweeds addition level. 3. Protein absorption and digestibility in seaweed addition treatments were significantly higher(p<0.05) than control group and protein contents of excreta in 0.5% and 1.0% treatments were reduced(p<0.05), which suggests effective protein metabolism for egg production. Increasing seaweeds addition reduces Ca and P contents in rectum and excreta, suggesting Ca and P utilization improvement in laying hens and lessening soil pollution. 4. In conclusion, seaweeds addition in layers' diet by 1.0% level improves egg-mass production and might be egg quality by increasing metabolism of protein, calcium and phosphorus.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.3
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• pp.384-394
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• 2001

In order to prevent pollution from animal waste, P, N and pharmacological level minerals should be properly managed. Microbial phytase has been used successfully to control P excretion. Activity of natural phytase in certain plant feedstuffs is high enough to be considered in feed formulation. Nitrogen control can be achieved through amino acid supplementation and protein restriction in the diet. Supplementation with carbohydrases reduces output of excreta as well as N. Ammonia release from the manure could be reduced by using a low crude protein diet along with the supplementation with probiotics products. Excretion of minerals used at pharmacological level can be reduced by using chelated forms. Cu and Zn in the form of methionine chelate have been successfully used in the broiler and pig diets.

• Korean Journal of Poultry Science
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• v.17 no.4
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• pp.310-317
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• 1990

An experiment was conducted to investigate the effects of adding extra dietary salt on the blood acid－base balance, water intake and egg quality in heat stressed layers. Eighteen 44－wk－old brown commercial layers(Dekalb Warren) were randomly alloted to 0.25 and 0.75% dietary salt treatments, with nine replicates per treatment and one bird per replicate. After 3 d of preliminary period at 13~$16^{\circ}C$(normal temperature), layers were subjected to 3 d of collection period. Then the room temperature was raised to 33－$35^{\circ}C$f(hot temperature), and another 3 d of collodion period was followed. The experiment was designed as a $2\times2$ factorial and analyzed as suck The ANOVA test and comparison among treatment means were done at 5% probability levels. Results obtained were summarized as follows. L The heat stressed layers decreased feed intake, and increased water intake and excreta moisture content significantly. The addition of extradietary salt significantly increased excreta moisture content 2. The heat stressed layers showed significantly higher blood pH and lower $pCO_2$ values than the control bird However, the addition of extra salt did not change any acid－base variables. 3. The egg weight and shell quality decreased significantly, and haugh unit increased significantly by the heat stress. However, these values were not affected by the salt addition. In summary, the heat stressed layers displayed respiratory alkalosis and poor egg quality, as expected However, the addition of ex03 salt to the diet failed to alleviated the heat stress in this layer experiment.

• Korean Journal of Poultry Science
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• v.12 no.2
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• pp.97-105
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• 1985

Fifteen 1-yr old roosters were employed in order to investigate the influence of various dietary fibers on the water and Na metabolism of chicken. Wheat bran, pure cellulose and ground rice straw replaced corn in the control diet at 10% level; Pectin replaced corn at 8% level. Three roosters were allotted per dietary treatment and one rooster per replicate. The limit of probability accepted as being significant was P$\leq$.05. The dry matter metabolizabilities of cellulose and rice straw diets were significantly lower, and those of control and pectin diets were significantly higher than that of the wheat bran diet. Pectin fed roosters seemed to drink more water than did the other birds. Also, excreta from roosters fed the pectin diet were significantly wetter than those from other treatments. However, no difference in water intake and fecal moisture were found when !he roosters were colostomized. It was also found that the colostomized birds drank more water than did intact ones. Data of water holding capacity and bulk density of dried excreta showed that cellulose, rice straw and wheat bran in decreasing order were strong in fibrous property. There were no significant differences in C$_{Na+}$ , Cosm and C$_{{H_2}O}$ among all treatments. When the values of urine volume, urine osmolality and C$_{{H_2}O}$ were put together, it can be concluded that 11 out of 15 roosters developed diuresis. More severe diuretic symptoms were developed in roosters when they were fed more fibrous diets. In conclusion, when the four dietary fibers were graded in terms of fibrous property, it turned out to be pectin, cellulose, rice straw and wheat bran in decreasing order.

• Journal of Animal Science and Technology
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• v.61 no.2
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• pp.87-97
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• 2019

Phytate induced excessive mineral excretion through poultry litter leads to poor performance and environmental pollution. Exogenous microbial phytase supplementation to poultry diets reduce the environmental excretion of nutrient and improve bird's performance. However, excessive dietary sodium (Na) level may hinder the phytase-mediated phytate hydrolysis and negate the beneficial effects of phytase. Therefore, this experiment was conducted to investigate the effects of different concentration dietary Na on phytase activity and subsequent impact on broiler performance, bone mineralisation and nutrient utilisation. In this study, six experimental diets, consisting of three different levels of Na (1.5, 2.5, or 3.5 g/kg) and two levels of microbial phytase (0 or 500 U/kg) were formulated by using $3{\times}2$ factorial design. The six experimental diets were offered to 360 day-old Ross 306 male chicks for 35 days, where, each experimental diet consisted of 6 replicates groups with 10 birds. Along with growth performance, nutrient utilization, intestinal enzyme activity, dry matter (DM) content of litter and mineral status in bone were analysed. Dietary Na and phytase had no effect on bode weight gain and feed intake. Birds on the low Na diet showed higher (p < 0.05) feed conversion ratio (FCR) than the mid-Na diets. High dietary Na adversely affected (p < 0.001) excreta DM content. Phytase supplementation to the high-Na diet increased (p < 0.01) the litter ammonia content. High dietary Na with phytase supplementation improved ($Na{\times}phytase$, p < 0.05) the AME value and ileal digestibility of Ca and Mg. The total tract retention of Ca, P, and Mg was reduced with high Na diet, which was counteracted by phytase supplementation ($Na{\times}phytase$, p < 0.001). The diets containing mid-level of Na improved (p < 0.001) the function of Na-K-ATPase and Mg-ATPase in the jejunum. The overall results indicate that high dietary Na did not affect phytase activity but influenced the nutrient utilization of birds, which was not reflected in bird overall performance.

• Korean Journal of Poultry Science
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• v.18 no.2
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• pp.97-119
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• 1991

Effect of Streptococcus faecium(SF) and an antibiotic, Colistin(Col), supplemented to diets singly or in combination, on the performances and changes of intestinal population of microflora of broiler chicks studied. A total of 252, day-old chicks(Arbor Acre) of mixed sex(M：F＝1：1) were alloted into six groups. A diet with no Col and SF was referred as a control diet. The basal diets were added with two levels of SF, 0.04 and 0.08%, singly or in combination with Col 10ppm Another diet was prepared by adding only Col 10 ppm. Numbers of the microorganism in diets added with SF 0.04% and 0.08% were 7$\times$10$^{4}$ and 1.4$\times$10$^{5}$ /g diet respectively The diets consisting of corn and soybean meal as major ingredients were fed for a period of seven weeks . During the feeding trial, fresh excreta were sampled at the end of every week in a sterilized condition to count microbial changes from each dietary group. Microbial changes of large intestine were also measured from nine birds sacrificed at the end of the 4th and 7th weeks each time per dietary group. Excreta from all the groups were also collected quantitatively at the end of 3rd and 6th weeks to measure digestibility of the diets, At the end of 7th week, nine birds from each group were also sacrificed to measure weight changes of gastrointestinal tracts . Average body weight gains of broilers fed the diets added with SF 0.08% (2.37kg) or SF 0. 08%+col 10ppm(2.34kg) were significantly larger than that of the control(2.18kg). The weight gains of the other groups were not statistically different from that of the control Feed/gain ratios of the supplemental groups were better than that of control (P＜0.05) except that of birds fed the diet added only with SF 0.04%. Digestibilities of nutrients such as dry matter, crude protein, crude fat and total carbohydrates were not altered by the consumption of the diets added with SF and/or Col throughout the whole feeding period. As expected, the numbers of Streptococci in the excreta from birds fed diets added with SF increased significantly with a statistical difference between groups with SF 0.04% and SF 0.08% most of the time. However. addition of Colistin to the diets supplemented with SF did not give any effects on the number of the microorganism. Numbers of coliforms in the excreta were apparently reduced by feeding the diets added with SF and/or Col(P＜0.05). There were, however, no additive effects observed between the two feed additives in this regard when supplementing Col to the SF diets. Distributions of intestinal microflora exhibited exactly the same pattern as those of the excreta. Length of small intestine of the birds fed diets added with SF 0.08% with or without Col 10 ppm became significantly longer with a range of about 10% than those of the birds fed diets without SF. However, the empty weight of the small inestine of the former group was lighter than that of control These changes resulted in a significant reduction in weight/unit length of the intestine of the birds fed diets supplemented with Col and SF singly or in combination. In overall conclusion, diet added with SF 0.08% appeared most effective in improving broiler performances. Colistin added at a level of 10ppm was not beneficial at all in itself or in combination with SF in terms of broiler performances or changes of intestinal microflora population. The efficacy of SF and Col could be attributed to the changes of wall thickness of the small intestine.