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Effect of Barley Yellow Mosaic Virus (BaYMV)- Infested Soil on the Agronomic Characters of Three Different Barley Varieties

  • Hyun, Jong-Nae;Hong, Yeon-Kyu;Lim, Sea-Gyu;Kim, Yang-Gil;Kim, Mi-Jung;Lee, Mi-Ja;Lee, Key-Woon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.4
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    • pp.427-433
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    • 2008
  • Barley Yellow Mosaic Virus (BaYMV) caused significant reduction in barley yield and is difficult to control due to alive parasitic soil-borne fungus, Palmyra gamines that transmits the, virus. Previous studies have indicated that a virus-free soil could be infested by using virus-contaminated farming machineneries and implements. For the further confirmation of this finding, different proportions of BaYMV-infested soil were mixed into virus free soil. Three barley varieties (Hordum vulgarae, cv "Olbori", "Baegdong" and "Sacheon 6") were sown in pots treated with different rate of P. graminis-infested soil ranging from 0% to 100% in October 20, 2001. Results showed that BaYMV infection increased as the rate of infested soil increased. Initial symptoms were observed in a pots treated with 10% infested soil in all the 3 varieties of barley. "Olbori" had about 5% infection in 20% infested soil and about 10% infection in 40% or 50% infested soil and about 20% infection in 60% infested soil. In "Baegdong", the trend of BYMV occurrence was similar with "Olbori" but the time of severe infection was earlier than "Olbori". BaYMV infection in "Sacheon 6" was even earlier than "Baegdong" with much more severe symptoms than "Baegdong". The growth rate of barley was affected by about 19-22% when grown in 20% infested soil. As the rate of BaYMV infested soil increased the heading date was delayed but the maturing date was early in "Olbori" and "Sacheon 6". Also, reduction rate of culm length in 3 varieties increased with increase of infested soil content. However, "Olbori" showed the highest reduction. "Sacheon 6", have been characterized with long spike length, however was significantly reduced as the infested soil increased. On the other hand, spike length of "Olbori" was not significantly affected despite of increased of infested soil. The reduction rate of 1000 kernel weight was higher in large kernel size cultivar "Sacheon 6" and "Olbori" than small kernel size "Baegdong" as increase of BaYMV-infested soil content.

A Comparative Study on 「Yanghwasorok」 and 『Zhangwuzhi』 - Focused on the Taste of Plants in Scholar's Garden, Korea and China - (「양화소록(養花小錄)」과 『장물지(長物志)』 화목류에 나타난 문인원림 취미 비교)

  • Park, Hee-Soung;Yun, Jia-Yan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.44 no.3
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    • pp.79-93
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    • 2016
  • The present study aimed to understand the taste of literati appearing in the Korean-Chinese garden by comparing "Yanghwasorok(養花小錄)" and "Zhangwuzhi(長物志)", which are one of the representative gardens in Korea and China. The main subject of comparison is plants; the research results are as follows. First, Gang Hui-an stated that the ultimate purpose of growing and appreciating the gardening plants is the completion of oneself, while Wen Zhen-heng(文震亨) used gardening plants as a means to practice a life of reclusiveness(隱逸). Second, Gang Hui-an claimed that growing plants is human's cultivation of virtue on the basis of Confucian view's gaining knowledge by the study of things(格物致知), whereas Wen Zhen-heng realized the taste of 'elegance(雅趣)' through form of plants or planting method. Third, although plant preference of literati of both countries is similar in many parts, there is a slight difference for putting gardening plants in pots and appreciating them. For example, even for selecting or placing pots, simplicity and lightness are characteristically reflected from Gang Hui-an while splendor and refinedness are characteristically shown from Wen Zhen-heng. Moreover, in light of the taste of appreciation of literati of the Song Dynasty(宋代), which is a sample of literati spirit, Gang Hui-an inherited the inner world of the spirit whereas Wen Zhen-heng expressed 'literati-ness' in visual images.

Effect of Bacterial Population from Rhizosphere of Various Foliage Plants on Removal of Indoor Volatile Organic Compounds (다양한 관엽식물의 근권부 박테리아 집단이 실내 휘발성 유기화합물질의 제거에 미치는 영향)

  • Chun, Se-Chul;Yoo, Mung-Hwa;Moon, Young-Sook;Shin, Mi-Ho;Son, Ki-Cheol;Chung, Ill-Min;Kays, Stanley J.
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.476-483
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    • 2010
  • Total bacterial populations were cultured from the Hydroball cultivation media in the rhizospheres of 9 different plants including $Hedera$ $helix$ L. and $Dracaena$ $deremensis$ cv. Warneckii Compacta, etc. These cultured bacterial populations were studied to test if the bacterial populations in the plant growing pots may play a role on removal of volatile organic compounds (VOCs) such as benzene and toluene in the air. To meet this objective, first, we tested the possibility of removal of VOCs by the cultured total bacteria alone. The residual rates of benzene by the inoculation of total bacterial populations from the different plant growth media were significantly different, ranging from 0.741-1.000 of $Spathiphyllum$ $wallisii$ 'Regal', $Pachira$ $aquatica$, $Ficus$ $elastica$, $Dieffenbachia$ sp. 'Marrianne' Hort., $Chamaedorea$ $elegans$, compared to the control with residual rate of 0.596 (LSD, $P$=0.05). This trend was also similar with toluene, depending on different plants. Based on these results, we inoculated the bacterial population cultured from $P.$ $aquatica$ into the plant-growing pots of $P.$ $aquatica$, $F.$ $elastica$, and $S.$ $podophyllum$ inside the chamber followed by the VOCs injection. The inoculated bacteria had significant effect on the removal of benzene and toluene, compared to the removal efficacy by the plants without inoculation, indicating that microbes in the rhizosphere could play a significant role on the removal of VOCs along with plants.

Growth of One-Year-Old Pot-Cultivated 'Fuji'/M.9 Apple Trees under Different Concentrations of Nitrogen Fertilization (질소시비농도에 따른 1년생 사과 'Fuji'/M.9 포트묘목의 수체 생장)

  • Ha, Woongyong;Shin, Hyunsuk;Lim, Heon-Kyu;Oh, Youngjae;Han, Hyeondae;Kim, Keumsun;Oh, Sewon;Kwon, Yeuseok;Kim, Daeil
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.499-508
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    • 2019
  • The study was carried out to investigate growth of 48.6-L pot-cultivated 1-year-old 'Fuji'/M.9 apple trees depending on different levels of nitrogen concentration. While rise in tree growth was paralleled with increase of nitrogen concentration, more than 32 mM of nitrogen rather restrained tree growth. In particular, growth of 16 mM of nitrogen treated trees was satisfied with criteria for production of high-quality pot-cultivated nursery stocks. Although mineral contents of leaves were higher in 8 and 16 mM nitrogen treatments than commonly recommended mineral contents in apple orchards, such somewhat surplus minerals could be helpful for tree growth after transplanting to apple orchards. In addition, our result indicated that soils of 8 and 16 mM of nitrogen treated pots met appropriate criteria for soil chemical property of apple orchards. Thus, in the light of tree growth, mineral contents of leaves, and soil chemical property in the pots, 16 mM of nitrogen treatment is considered to be suitable for production of 1-year-old 'Fuji'/M.9 apple potted trees.

The Effects of Soybean Cultivation on Soil Microorganism Activity (콩 재배가 토양 미생물 군집 활성도에 미치는 영향)

  • Bak, Gyeryeong;Lee, Gyejun;Kim, Taeyoung
    • Korean Journal of Environmental Agriculture
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    • v.38 no.2
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    • pp.76-82
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    • 2019
  • BACKGROUND: For sustainable agriculture, there are various agricultural practices including low input. Over the last few decades high input of chemical fertilizer and compounds results in environmental pollution and deterioration of soil fertility. Soybean (Glycine max L.) is well known eco-friendly crop due to their symbionts. Soybean has a relationship with nitrogen fixation bacteria called rhizobia. In this research work, we investigated effects of soybean cultivation on soil microorganism activities. METHODS AND RESULTS: Experiments were conducted in pots and potato cultivation was used as reference. Soil chemical properties were analyzed considering soil nutrient over cropping period. For the soil microbial community analysis, dehydrogenase activity analysis (DHA) analyzed along with denaturing gradient gel electrophoresis. The results showed that higher soil organic matter in the soybean cultivation soil than in the potato cultivation soil. Available $P_2O_5$ concentration increased gradually in both pots but showed higher value in the potato cultivation soil. DHA value implying microbial activities showed higher value in the soybean cultivation soil over all cropping period. CONCLUSION: The cause of high microbial activity in the soybean cultivation soil was considered to the effects of some specific microorganisms related to soybean cultivation. Therefore, the availability of soybean cultivation for sustainable agriculture should be encouraged in terms of microorganism community activity in soil.

Exposure Assessment of Heavy Metals Migrated from Glassware on the Korean Market (국내 유통 식품용 유리제의 중금속 노출 평가)

  • Kim, Eunbee;Hwang, Joung Boon;Lee, Jung Eun;Choi, Jae Chun;Park, Se-Jong;Lee, Jong Kwon
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.28 no.1
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    • pp.15-21
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    • 2022
  • The purpose of our study was to investigate the migration level of lead (Pb), cadmium (Cd), and barium (Ba) from glassware into a food simulant and to evaluate the exposure of each element. The test articles were glassware, including tableware, pots, and other containers. Pb, Cd, and Ba were analysed by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The analytical performance of the method was validated in terms of its linearity, limit of detection (LOD), limit of quantification (LOQ), recovery, precision, and uncertainty. The monitoring was performed for 110 samples such as glass cups, containers, pots, and bottles. a food simulant. Migration test was conducted at 25? for 24 hours in a dark place using 4% acetic acid as a food simulant. Based on the data; exposure assessment was carried out to compare the estimated daily intake (EDI) to the human safety criteria. The risk levels of Pb and Ba determined in this study were approximately 1.9% and 0.3% of the provisional tolerable weekly intake (PTWI) and tolerable daily intake (TDI) value, respectively, thereby indicating a low exposure to the population.

Mass Propagation of Sundew, Drosera rotundifolia L. through Shoot Culture

  • Jang, Gi-Won;Park, Ro-Dong
    • Journal of Plant Biotechnology
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    • v.1 no.2
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    • pp.97-100
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    • 1999
  • In order to establish in vitro propagation method of sundew, Drosera rotundifolia L., the effects of MS medium concentration, cytokinin type and concentration, pH, and auxin type and concentration on shoot proliferation and root formation were investigated using shoots at 3 month after seed germination. The highest shoot production was obtained with the half strength of MS ($\frac{1}{2}$ MS) medium than with any other strength of MS medium tested. Addition of kinetin or BA in $\frac{1}{2}$ MS medium was strongly suppressed shoot proliferation. The suppression of shoot proliferation was more effective in BA-supplemented $\frac{1}{2}$ MS medium than kinetin-supplemented. The optimum pH of the media for shoot proliferation was pH 5.7-6.7. Shoots were subcultured in $\frac{1}{2}$ MS medium supplemented with 0.5mg/L 2,4-D for rooting every 8 weeks. All subcultured shoots produced extensive root systems after 5 to 6 week culture. Plantlets after root development were planted in plastic pots filled with moss. The survival rate of plantlets was almost 100%. On subculturing every 8 weeks, hundreds of the plants were propagated from a single plant within a year.

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Plant Regeneration of B.juncea Through Plant Tissue and Protoplast Culture

  • Lian, Yu-Ji;Lim, Hak-Tae
    • Journal of Plant Biotechnology
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    • v.3 no.1
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    • pp.27-31
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    • 2001
  • New types of cytoplasmic male sterility in Brassica species would be very useful for the production of F$_1$, hybrid seeds. Leaves and stems of rapid cycling stock of B.juncea (CrGC4-3) containing Anand CMS were used as experimental materials for plant regeneration from protoplast culture. Very high plant regeneration rate (85%) was found in the Kao & Michayluk medium supplemented with 2 mg/L zeatin, 0.5 mg/L BAP, and 1 mg/L NAA when only leaf, not stem, segments were cultured. Protoplasts were isolated from leaves using mixtures of enzymes (1% Cellulycin, 0.5% Macerozyme) in 0.4 M mannitol and 50 mM $CaCl_2$.$2H_2$O. Mcrocalli induced from protoplasts were transferred to the shoot regeneration medium containing 2 mg/L BAP, 2 mg/L zeatin, and 0.5 mg/L NAA. After 60 days of initial protoplast culture, regenerated plantlets were obtained, acclimatized, transplanted into the pots, and grown up to the flowering stage.

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Isolation and Characterization of Plant Growth Promoting Rhizobacteria From Button Mushroom Compost

  • Oh, Sung-Hoon;Lee, Chang-Jung;Yoon, Min-Ho
    • Korean Journal of Agricultural Science
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    • v.43 no.1
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    • pp.100-108
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    • 2016
  • An auxin-producing bacteria (strain 5-1) was isolated from button mushroom compost in Boryeong-Si, Chungcheongnam-Do. The 5-1 strain was classified as a novel strain of Enterobacter aerogenes based on chemotaxonomic and phylogenetic analyses. The isolated E. aerogenes 5-1 was confirmed to produce indole-3-acetic acid (IAA), one of the auxin hormones, using TLC and HPLC analyses. When the concentration of IAA was assessed by performing HPLC quantitative analysis, a maximum concentration of IAA of $109.9mgL^{-1}$ was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 24 h at $35^{\circ}C$. Acidification of the culture was deemed caused by an increase of IAA because a negative relationship between IAA production and pH was observed. Supplementation with a known precursor of IAA production, L-tryptophan, appeared to induce maximal production at 0.1% concentration, but it reduced production at concentrations above 0.2%. To investigate the growth-promoting effects to crops, the culture broth of E. aerogenes 5-1 was used to inoculate water cultures and seed pots of mung bean and lettuce. In consequence, adventitious root induction and root growth of mung bean and lettuce were two times higher than those of the control.

Disease Suppressive Mechanisms of Antagonistic Bacteria against Phytophthorn capsici causing Phytophthora Blight of Pepper

  • Kim, Hye-Sook;Kim, Ki-Deok
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.93.2-94
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    • 2003
  • In our previous studies, we selected three antagonistic bacteria, KJ1R5, KJ2C12, and KJ9C8 against Phytophthora capsici, the casual agent of Phytophthora blight of pepper. For elucidating production, root colonization, and total microbial activity were investigated. The dual culture assay was accomplished to elucidate existence of antibiotics. In this assay, any antagonistic bacteria did not inhibit growth of six important fungal plant pathogens, suggesting that these antagonists do not produce antibiotics. root surface or rhizosphere soil colonizations were examined with spontaneous rifampicin-resistant mutants equal to antagonistic ability of wild types. KJ2C12 colonized consistently rhizosphere soil while yellowish colonies of KJ1R5 and KJ9C8 well colonized root surfaces and rhizosphere soil. Total microbial activity in pots treated with the antagonistic bacteria was measured using fluorescein diacetate hydrolysis. total microbial activity of three antagonistic bacteria treatments was significantly higher than that of buffer-treated control until 4days after treatment. However, total microbial activity of treatment of three antagonistic bacteria decreased after 7 days. These results indicate that the antagonistic bacteria, KJ1R5 and KJ9C8 colonized and protected roots well against Phytophthora blight of pepper through competition of infection courts, especially competitions.

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