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Evaluation of Tomato Genetic Resources for the Development of Resistance Breeding Lines against Late Blight (잎마름역병 저항성 육종을 위한 토마토 유전자원의 저항성 평가)

  • Kim, Byung-Sup
    • Research in Plant Disease
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    • v.18 no.1
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    • pp.35-39
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    • 2012
  • Occurrence of tomato late blight (Phytophthora infestans) has caused significant losses in tomato yield in all over the world. Evaluation of the level of resistance in tomato gene resources for main breeding and initiation of the resistance breeding program are important for control of this disease. Resistant assay of 78 tomato cultivars/lines to late blight in pots and field experiment was carried out under controlled and natural conditions in 2009. All commercial cultivars including 'Legend' were susceptible. However, 10 lines including KNU-2, KNU-6-1, KNU-11, KNU-13, KNU-14-1 lines distributed from University of California, Riverside and L3708, $AV107-4{\times}L3708$, $07-15{\times}L3708$, $BS67{\times}L3708$ lines which have resistant gene Ph-3 and $06-9-62A{\times}06-9-62A$ were highly resistant to late blight. These highly resistant lines can be used as resources of resistance to late blight in a tomato breeding program in future.

Yield Decrease of Tall Fescue ( Festuca arundinacea Schreb. ) by Pathogenic Fungi and its Control by Antagonistic Bacteria (병원성사상균에 의한 Tall Fescue ( Festuca arundinacea Schreb. ) 의 수량 손실과 길항 미생물에 의한 그 방제)

  • Choi, Ki-Chun;Song, Chae-Eun;Lee, Joung-Kyong;Kim, Jong-Hyun;Rhee, Young-Hwan;Youn, Chang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.18 no.2
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    • pp.133-142
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    • 1998
  • This study was conducted to investigate the effects of antagonistic bacteria and pathogenic fungi on the growth of tall fescue(Festuca arundinacea Schreb.) in continuous cropping soil(CCS) and non-continuous cropping soil(NCCS). Tall fescue was established by seeding into pots of 11 cm in diameter and 9 cm in depth containing 1 : 1 mixture of soil and vermiculite, and cultivated at pots with antagonistic bacteria and pathogenic fungi in a vinyl house. The bacteria used in this study were Bacillus subtilis and hsants. B. subtilis was isolated and identified kern forage rhizosphere soil and fusants were isolated through cell hsion from B. subtilis and B. thwingiensis. B. subtilis was named as B. subtilis 101 and hsants were named as F-3, F-7 and F-8. In dark culture experiment, tall fescue inoculated with the antagonistic bacteria lived longer than that of control in both CCS and NCCS. However, tall fescue of CCS lived shorter than that of NCCS. Dry weight of tall fescue inoculated with the antagonistic bacteria was higher than that of tall fescue inoculated with pathogenic hngi in both CCS and NCCS(P< 0.05), and the antagonistic bacteria showed positive effects on the growth of tall fescue. However, Dry weight of tall fescue was decreased by the inoculation of the pathogenic b g i in both CCS and NCCS(P< 0.05).

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The Assesment of Heavy metal Pollution in Sewage Sludeges from Gully Pots Alongside Some Main Roads in Seoul, Korea (서울시 도로변의 빗물받이에서 체취한 하수슬러지의 중금속 오염 평가)

  • 이평구
    • Economic and Environmental Geology
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    • v.32 no.6
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    • pp.633-644
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    • 1999
  • Gully pot is a part of urban darainage system to butter the runoff water fluxes from road to rivers and to minimize environmental pollution by prevending over the surrounding area. A series of studies have been carried out concerning the physicochemical characteristics of the sewage sludge sediments sttling down in such a gully pot in order to over to evaluate the contamination for heavy metals such as pb, Zn, Cu and Cd. The roadside soil and sewage sludge samples from gully pots were characterized by XRD analyses and sequential extraction : Zn 2595.7$\mu$g/g; Cd9.8$\mu$/g; Cu602.5$\mu$g/g; Pb260.0$\mu$g/g),because of a long-term accumulation of vehicle- and industrial-related pollutants. Mean Zn concentration in Yeouido (3873$\mu$g/g) and Junggu(3262$\mu$g/g)areas are 4-5 times higher than those in Dobonggu area, suggesting that Zn may be derived from automobile traffic (including the rubber of automobile tires). The mean concentrations of Cu and Cr are very significantly high in Junggu and Gurogu areas, possibly due to the industrial activities in these areas. The low Pb levels throughtotut the whole study areas in Seoul can be accounted for the use of unleaded gasoline since 1987. Sequential extraction experiments illustrate that a major part of Zn is bound to FII and FIII, representing about 88% of the total Zn concentration. Fraction IV, related to orgnic matter, is mostly significant for Cu accounting for 60% of the total Cu, and FII is next in importance. The main carriers of Pb are the fractions III, II and FIV, and in that order. The behavior of Cd is quite different from other elements (Zn, Cu, Pb), and most of the Cd is associated with FV. Changes in the physicochemical environments (such as acidification) may result in severe environmental pollution of surface water and rivers with respect to heavy metals (especially Zn and Cu).

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Vocational Rehabilitation of People with Mental Disabilities by Repeated Training of Horticultural Activities

  • Seol, Ga Ae;Yun, Suk Young;Choi, Byung Jin;Jang, Eun Jin;Jang, Hyun Hee
    • Journal of People, Plants, and Environment
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    • v.23 no.1
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    • pp.67-75
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    • 2020
  • The purpose of this study was to develop an effective horticultural activity program for the vocational rehabilitation of people with mental disabilities. The subjects of this study were seven persons with mental disabilities in the vocational rehabilitation team of a public health center located in G city, Gyeongsangbuk-do. A total of 10 horticultural programs were held from October 10 to December 11, 2018. Five programs were selected among those that were easy for the persons with mental illness to handle and their preference was high. In each session, the same task was repeated 10 times and finally changes in hand functions and working speed were measured. As a result, the assembly task of the Purdue Pegboard test was 23.6 (SD = 10.5) before the program, 26.6 (SD = 11.5) after planting, 27.7 (SD = 12.2) after wrapping pots, and 28.0 (SD = 13.9) after making mini flower baskets, 26.9 (SD = 12.0) after wrapping a single flower, and 29.1 (SD = 11.9) after making corsages. There was a significant difference between the five programs (p = .016). As a result of measuring the time taken to produce the same 10 products, the average planting time was 9.9 (SD = 4.0) minutes in the first class and 6.0 (SD = 2.3) minutes in the second class (p = .018), and making mini flower baskets was reduced from 35.2 (SD = 10.1) minutes to 21.0 (SD = 7.7) minutes (p = .018), wrapping a single flower from 23.3 (SD = 7.9) minutes to 16.2 (SD = 7.3) minutes (p = .043), and making corsages from 53.6 (SD = 15.9) to 40.8 (SD = 16.8) minutes (p = .043). The working time was significantly shortened in the second class compared to the first class. The class for wrapping pots was shortened from 52.7 (SD = 11.7) to 49.6 (SD = 17.8) minutes, but there was no significant difference (p = .398).

Synthesis of Nano Sulfur/Chitosan-Copper Complex and Its Nematicidal Effect against Meloidogyne incognita In Vitro and on Coffee Pots

  • Hong Nhung Nguyen;Phuoc Tho Tran;Nghiem Anh Tuan Le;Quoc Hien Nguyen;Duy Du Bui
    • The Plant Pathology Journal
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    • v.40 no.3
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    • pp.261-271
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    • 2024
  • Sulfur is one of the inorganic elements used by plants to develop and produce phytoalexin to resist certain diseases. This study reported a method for preparing a material for plant disease resistance. Sulfur nanoparticles (SNPs) stabilized in the chitosan-Cu2+ (CS-Cu2+) complex were synthesized by hydrolysis of Na2S2O3 in an acidic medium. The obtained SNPs/CS-Cu2+ complex consisting of 0.32% S, 4% CS, and 0.7% Cu (w/v), contained SNPs with an average size of ~28 nm as measured by transmission electron microscopy images. The X-ray diffraction pattern of the SNPs/CS-Cu2+ complex showed that SNPs had orthorhombic crystal structures. Interaction between SNPs and the CS-Cu2+ complex was also investigated by ultraviolet-visible. Results in vitro nematicidal effect of materials against Meloidogyne incognita showed that SNPs/CS-Cu2+ complex was more effective in killing second-stage juveniles (J2) nematodes and inhibiting egg hatching than that of CS and CS-Cu2+ complex. The values of LC50 in killing J2 nematodes and EC50 in inhibiting egg hatching of SNPs/CS-Cu2+ complex were 75 and 51 mg/l, respectively. These values were lower than those of CS and the CS-Cu2+ complex. The test results on the nematicidal effect against M. incognita on coffee pots showed that the SNPs/CS-Cu2+ complex was 100% effective at a concentration of 150 mg/l. Therefore, the SNPs/CS-Cu2+ complex could be considered as a biochemical material with potential for agricultural applications to control root-knot nematodes.

The Effect of Temperature on the Colony Development of Bombus ignitus Smith (호박벌(Bombus ignitus Smith)의 봉군발육에 미치는 영향)

  • 이상범;마영일;배태웅
    • Journal of Life Science
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    • v.9 no.4
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    • pp.395-407
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    • 1999
  • The queens of Korean native bumblebee species, Bombus ignitus were collected from the field in the spring of 1997 and reared under the various temperatures as 15$^{\circ}C$$\pm$1$^{\circ}C$, 2$0^{\circ}C$$\pm$1$^{\circ}C$, $25^{\circ}C$ $\pm$1$^{\circ}C$, 3$0^{\circ}C$$\pm$1$^{\circ}C$, 60%$\pm$5% of relative humidity and 14L : 10D, to investigate their ecological characteristics, as comparing with control(29$^{\circ}C$$\pm$1$^{\circ}C$, 60%$\pm$5% of relative humidity and red light). As a result, eggs were laid by foundation queen at 15$^{\circ}C$, but they didn't hatched out while at 2$0^{\circ}C$ eggs were laid by them and they hatched out. When a foundation queen laid eggs at $25^{\circ}C$ and they all were hatched out and normally grown. Finally they were developed to the normal colony. B. ignitus worker survived for 77days at 2$0^{\circ}C$, about 69days at $25^{\circ}C$, about 68days at 3$0^{\circ}C$ and about 63days at 29$^{\circ}C$(control). The first brood workers emerged in 25-27days from the egg which a foundation queen laid. At the various temperatures, the captive queens stand to lay eggs in about 18days at 15$^{\circ}C$, 15days at 2$0^{\circ}C$, 11days at $25^{\circ}C$, 11days at 3$0^{\circ}C$ and 4days in control. The first worker and the first drone from the egg cell of the collected queens appeared in about 27days and 72days at 2$0^{\circ}C$, 26days and 88days in $25^{\circ}C$, 24days and 65days at 3$0^{\circ}C$, 25days and 71days in control, respectively. In colony foundation, 33% of the collected queen at 2$0^{\circ}C$ and $25^{\circ}C$, 100% of the disposed queen at 3$0^{\circ}C$ and in control, and 67% of them at 3$0^{\circ}C$ and in control both produced new queens in 66days and 88days, respectively. The life span of the colony founded covered fro about 3 months at $25^{\circ}C$, 3$0^{\circ}C$ and in control. At lower temperatures, the life span of queen is shorter; 2 months at 15$^{\circ}C$ and 3 months at 2$0^{\circ}C$, respectively. A colony which normally developed, varied in size with rearing temperatures; about 20heads at 2$0^{\circ}C$, 482heads at $25^{\circ}C$, 330heads at 3$0^{\circ}C$ and 452heads in control. A foundation queen monthly oviposited 1.5egg cells at 15$^{\circ}C$, 3.0egg cells at 2 $0^{\circ}C$, 21.7egg cells at $25^{\circ}C$, 42.3egg cells at 3$0^{\circ}C$ and 47.0egg cells in control. As a colony developed in June and July, egg cells as well as daily average cells increased in number, as compared to those in May and August. Also, in June and July, interval(days) of egg laying is shorter than in May and August. Number of nectar pots during the colony development varied with rearing temperatures; 31pots at $25^{\circ}C$, 39pots at 3$0^{\circ}C$, 23pots in control. The emergence of new queen showed a distinctively different two patterns; early emerging type and late emerging type. Workers are rapidly increased from early in July to early in August at $25^{\circ}C$, but at 3$0^{\circ}C$ and in control, emergence of workers are remarkably increased from the middle of June and last until July. No new queen emerged at 15$^{\circ}C$, 2$0^{\circ}C$ and $25^{\circ}C$. New queens at 3$0^{\circ}C$ and in control emerged between late in June and early in July. Thus emergence of new queen was temperature dependent.

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Effect of Amounts of Pisolithus tinctorius Spores and Fertilizer on the Growth of Potted Pinus densiflora Seedlings (모래밭 버섯 포자접종량(胞子接種量)과 시비량(施肥量)에 따른 소나무 화분파종묘(花盆播種苗)의 생장촉진효과(生長促進効果))

  • Koo, Chang Duck;Lee, Won Kyu;Lee, Chun Yong;Park, Seung Kull
    • Journal of Korean Society of Forest Science
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    • v.72 no.1
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    • pp.32-36
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    • 1986
  • The effect of different inoculation amounts of Pisolithus tinctorius (Pt) spores and fertilizers on the growth of Pinus densiflora seedlings grown in fumigated or nonfumigated soils were tested in the: polyethylene pots. To infest the pot soil with Pt spores, the mixture of the spores with sterilized nursery soil was used after seeding. Eighteen months after inoculation, mycorrhizal formation was 42-70% in fumigated plus Korean-Pt inoculated pots, 60-70% in fumigated plus U.S.-Pt inoculated pots, and less than 1% in non-fumigated, Korean-Pt inoculated pots. Growth enhancement effect of Pt spore inoculation was shown on only fumigated soil and the inoculation increased the seedlings height (8-38%), stem diameter (9-40%) and dry weight (6-73%). Especially 0.4g per pot application rate increased the height (30-31%), stem diameter (23-28%) and dry weight (56-69%), while the 0.2g U.S. Pt spore per pot rate increased 26-38, 17-20 and 58-60%, for height, stem diameter, and dry weight respectively. At 1X fertilizer application rate (urea 2g, fused superphoshate 4g, and potassium chloride 1g per pot), the 0.4g per pot rate resulted in more dry weight by 18% than that at 1/2X rate. The result indicated that there is a need for further research on inoculating nonfumigated soil with Pt and that appropriate application rates of fertilizers and Pt spores are 1X and 0.2-0.4g per pot on fumigated soil, respectively.

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Characterization of Myrothecium roridum Isolated from Imported Anthurium Plant Culture Medium

  • Kwon, Hyuk Woo;Kim, Jun Young;Choi, Min Ah;Son, Seung Yeol;Kim, Seong Hwan
    • Mycobiology
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    • v.42 no.1
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    • pp.82-85
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    • 2014
  • During an investigation of microorganisms and pests in plant culture media from imported anthurium pots, a fungal isolate (DUCC4002) was detected. Based on its morphological characters including colony shape on potato dextrose agar, the microstructures of spores observed by light and scanning electron microscopy and the results of phylogenetic analysis using an internal transcribed spacer rDNA sequence, the fungal isolate was identified as Myrothecium roridum. Pathogenicity testing on anthurium leaves revealed that the fungus could colonize and produce sporodochia on the inoculated leaves. This is the first report of M. roridum detected in imported plant culture medium in Korea.

A Survey on the Farmer's Life in Yanbian, China (중국 연변 조선족 농업인 생활실태 조사)

  • Choi, Yoon-Ji;Gim, Gyung-Mee;Lee, Jin-Young
    • Journal of Agricultural Extension & Community Development
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    • v.13 no.1
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    • pp.185-193
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    • 2006
  • The study is designed to understand on the farmer's life in Yanbian, China. The major findings are: 1) The people in farmhouse use wood as a fuel for cooking and the method of cooking is traditional style using their iron pots. The floor heating of the house and cooking food can be carried out simultaneously. Most of the farmer use pump system. 2) Korean traditional foods should be inherited and they usually eat rice. Kimchi, Soy-sauce, and Hot-pepper paste are mostly made at home. 3) Housewives do actively participate in income management. Results indicated that overall Korean immigrants in China maintain their ethnic identity, ethnic language and culture.

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Pseudomonas avenae Causing Bacterial Brown Stripe Disease of Rice in Korea (Pseudomonas avenae에 의한 벼$\cdot$세균성 줄무늬병)

  • Shakya D.D.;Chung Hoo Sup
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.38-43
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    • 1985
  • A bacterial brown stripe disease new to Korea was noted for the first time on rice seedlings grown in the nursery pots and in a field nursery. Artificial hypodermic injection and pricking inoculation with isolates obtained from lesions of naturally infected plants produced symptoms similar to those occurring under natural condition. Among eleven species of ten genera of gramineous plants Echinochloa crusgalli, Digitaria sanguinalis and Setaria viridis were the new hosts for the pathogen. On the basis of bacteriological and biochemical tests of isolates from infected rice seedlings, the causal bacterium is considered to be Pseudomonas avenae Manns.

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