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http://dx.doi.org/10.5941/MYCO.2014.42.1.82

Characterization of Myrothecium roridum Isolated from Imported Anthurium Plant Culture Medium  

Kwon, Hyuk Woo (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Kim, Jun Young (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Choi, Min Ah (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Son, Seung Yeol (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Kim, Seong Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Publication Information
Mycobiology / v.42, no.1, 2014 , pp. 82-85 More about this Journal
Abstract
During an investigation of microorganisms and pests in plant culture media from imported anthurium pots, a fungal isolate (DUCC4002) was detected. Based on its morphological characters including colony shape on potato dextrose agar, the microstructures of spores observed by light and scanning electron microscopy and the results of phylogenetic analysis using an internal transcribed spacer rDNA sequence, the fungal isolate was identified as Myrothecium roridum. Pathogenicity testing on anthurium leaves revealed that the fungus could colonize and produce sporodochia on the inoculated leaves. This is the first report of M. roridum detected in imported plant culture medium in Korea.
Keywords
Anthurium; Import; Myrothecium roridum; Plant culture medium;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Kim DK, Bae DW, Lee SC, Han KS, Kim HK. Detection of Myrothecium leaf spot, a new disease of watermelon. Plant Pathol J 2003;19:200-2.   DOI   ScienceOn
2 White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
3 Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673-80.   DOI   ScienceOn
4 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.   DOI   ScienceOn
5 Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
6 Than PP, Jeewon R, Hyde KD, Pongsupasamit S, Mongkolporn O, Taylor PW. Characterization and pathogenicity of Colletotrichum species associated with anthracnose disease on chili (Capsicum spp.) in Thailand. Plant Pathol 2008;57:562-72.   DOI   ScienceOn
7 Kim SH, Uzunovic A, Breuil C. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR. Appl Environ Microbiol 1999;65:287-90.
8 Murakami R, Yasui H, Shirata A, Kato A. Production of myrotoxin B by Myrothecium roridum isolated from Myrothecium leaf spot of mulberry in Japan. J Seric Sci Jpn 1999;68:469-77.
9 Murakami R, Shirata A. Myrotoxin B detection from mulberry leaves infected with Myrothecium roridum, cause Myrothecium leaf spot of mulberry, and possible role in pathogenicity. Jpn J Phytopathol 2005;71:91-100.   DOI
10 Fergus CL. Myrothecium roridum on Gardenia. Mycologia 1957;49:124-7.   DOI
11 Yum KJ, Park EW. Soybean leafspot disease caused by Myrothecium roridum Tode ex Fries. Korean J Plant Pathol 1990;6:313-7.
12 Chen J, McConnell DB, Henny RJ, Everitt KC. Cultural guidelines for commercial production of interiorscape Anthurium. University of Florida, IFAS extension, EHN956. Gainesville: University of Florida; 2003.
13 Dufour L, Guérin V. Growth, developmental features and flower production of Anthurium andreanum Lind. in tropical conditions. Sci Hortic 2003;98:25-35.   DOI   ScienceOn
14 Jouen E, Laurent P, Robène-Soustrade I, Gagnevin L, Pruvost O, Hostachy B, Gateblé G, Amice R, Imbert F. First report in New Caledonia of bacterial blight of anthurium caused by Xanthomonas axonopodis pv. dieffenbachiae. Plant Dis 2007; 91:462.
15 Baniqued NC. Survey and identification of important disease attacking anthurium and chrysanthemum. Philipp J Plant Ind 1991;56:11-33.
16 Ann PJ. Phytophthora diseases of ornamental plants in Araceae in Taiwan. Plant Pathol Bull 1992;1:79-89.
17 Quezado Duval AM, Henz GP, Paz-Lima ML, Medeiros AR, Miranda BE, Pfenning LH, Reis A. New hosts of Myrothecium spp. in Brazil and a preliminary in vitro assay of fungicides. Braz J Microbiol 2010;41:246-52.   DOI
18 Baudoin AB. Laboratory exercises in plant pathology: an instructional kit. St. Paul: American Phytopathological Society; 1988. p. 3-4.
19 Lee HB, Kim JC, Hong KS, Kim CJ. Evaluation of a fungal strain, Myrothecium roridum F0252, as a bioherbicide agent. Plant Pathol J 2008;24:453-60.   DOI   ScienceOn
20 Yun YH, Hyun MW, Suh DY, Kim YM, Kim SH. Identification and characterization of Eurotium rubrum isolated from Meju in Korea. Mycobiology 2009;37:251-7.   DOI