• Journal of The Korean Society of Grassland and Forage Science
• /
• v.35 no.3
• /
• pp.257-263
• /
• 2015

This study was conducted to assess the potential of using NIRS to accurately determine the chemical composition and fermentation parameters in fresh coarse sorghum and sudangrass silage. Near Infrared Spectroscopy (NIRS) has been increasingly used as a rapid and accurate method to analyze the quality of cereals and dried animal forage. However, silage analysis by NIRS has a limitation in analyzing dried and ground samples in farm-scale applications because the fermentative products are lost during the drying process. Fresh coarse silage samples were scanned at 1 nm intervals over the wavelength range of 680~2500 nm, and the optical data were obtained as log 1/Reflectance (log 1/R). The spectral data were regressed, using partial least squares (PLS) multivariate analysis in conjunction with first and second order derivatization, with a scatter correction procedure (standard normal variate and detrend (SNV&D)) to reduce the effect of extraneous noise. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The results of this study showed that NIRS predicted the chemical constituents with a high degree of accuracy (i.e. the correlation coefficient of cross validation ($R^2{_{cv}}$) ranged from 0.86~0.96), except for crude ash which had an $R^2{_{cv}}$ of 0.68. Comparison of the mathematical treatments for raw spectra showed that the second-order derivatization procedure produced the best result for all the treatments, except for neutral detergent fiber (NDF). The best mathematical treatment for moisture, acid detergent fiber (ADF), crude protein (CP) and pH was 2,16,16 respectively while the best mathematical treatment for crude ash, lactic acid and total acid was 2,8,8 respectively. The calibrations of fermentation products produced poorer calibrations (RPD < 2.5) with acetic and butyric acid. The pH, lactic acid and total acids were predicted with considerable accuracy at $R^2{_{cv}}$ 0.72~0.77. This study indicated that NIRS calibrations based on fresh coarse sorghum and sudangrass silage spectra have the capability of assessing the forage quality control

• Korean Journal of Environmental Agriculture
• /
• v.20 no.3
• /
• pp.143-149
• /
• 2001

A contact oxidation canal system with sedimentation basin was installed to study the efficiency of water quality purification. The primary sedimentation basin with 60 min of HRT (Hydraulic Retention Time) included in the system was aimed to sediment pollutants in the water and the deposit being released by the drainage culvert located at the bottom of the system. The oxidation canal aerated by nozzle was to contact the pollutants and oxygen in the surface of plastic filter to purify the water. Discharge, HRT, length of the oxidation canal were $200\;m^3/day$, 90 min, 20 m, respectively. The treatment efficiency of total nitrogen was lower compared with other water quality items such as SS, BOD, TP because the anoxic condition for denitrification was not ensured after the oxidation canal. However, $25%{\sim}89.6%$ of SS, $75.0%{\sim}91.5%$ of BOD, $44.3%{\sim}95.3%$ of TP were removed in this system. Overall, the results indicates that this system appears to have a potential capability for water quality improvement of the reservoirs or the canals in the agricultural watershed.

• Journal of Ginseng Research
• /
• v.44 no.4
• /
• pp.664-671
• /
• 2020

Background: Ginsenoside compound-Mc1 (Mc1) is a member of the deglycosylated ginsenosides obtained from ginseng extract. Although several ginsenosides have a cardioprotective effect, this has not been demonstrated in ginsenoside Mc1. Methods: We treated H9c2 cells with hydrogen peroxide (H₂O₂) and ginsenoside Mc1 to evaluate the antioxidant effects of Mc1. The levels of antioxidant molecules, catalase, and superoxide dismutase 2 (SOD2) were measured, and cell viability was determined using the Bcl2-associated X protein (Bax):B-cell lymphoma-extra large ratio, a cytotoxicity assay, and flow cytometry. We generated mice with high-fat diet (HFD)-induced obesity using ginsenoside Mc1 and assessed their heart tissues to evaluate the antioxidant effect and the fibrosis-reducing capability of ginsenoside Mc1. Results: Ginsenoside Mc1 significantly increased the level of phosphorylated AMP-activated protein kinase (AMPK) in the H9c2 cells. The expression levels of catalase and SOD2 increased significantly after treatment with ginsenoside Mc1, resulting in a decrease in the production of H₂O₂-mediated reactive oxygen species. Treatment with ginsenoside Mc1 also significantly reduced the H₂O₂-mediated elevation of the Bax:Bcl2 ratio and the number of DNA-damaged cells, which was significantly attenuated by treatment with an AMPK inhibitor. Consistent with the in vitro data, ginsenoside Mc1 upregulated the levels of catalase and SOD2 and decreased the Bax:B-cell lymphoma-extra large ratio and caspase-3 activity in the heart tissues of HFD-induced obese mice, resulting in reduced collagen deposition. Conclusion: Ginsenoside Mc1 decreases oxidative stress and increases cell viability in H9c2 cells and the heart tissue isolated from HFD-fed mice via an AMPK-dependent mechanism, suggesting its potential as a novel therapeutic agent for oxidative stress-related cardiac diseases.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.8 no.1
• /
• pp.1-7
• /
• 2010

In the present work, an electrowinning process in the LiCl-KCl/Cd system is considered to model and analyze the electrotransport of the actinide and rare-earth elements. A simple dynamic modeling of this process was performed by taking into account the material balances and diffusion-controlled electrochemical reactions in a diffusion boundary layer at an electrode interface between the molten salt electrolyte and liquid cadmium cathode. The proposed modeling approach was based on the half-cell reduction reactions of metal chloride occurring on the cathode. This model demonstrated a capability for the prediction of the concentration behaviors, a faradic current of each element and an electrochemical potential as function of the time up to the corresponding electrotransport satisfying a given applied current based on a galvanostatic electrolysis. The results of selected case studies including five elements (U, Pu, Am, La, Nd) system are shown, and a preliminary simulation is carried out to show how the model can be used to understand the electrochemical characteristics and provide better information for developing an advanced electrowinner.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.15 no.1
• /
• pp.197-210
• /
• 2012

The KOrea Multi-Purpose SATellite(KOMPSAT)-2 has a capability to provide a cross-track stereo imagery using two different orbits for generating various spatial information. However, in order to fully realize the potential of the KOMPSAT-2 stereo imagery in terms of mapping, various tests are necessary. The purpose of this study is to evaluate the possibility of mapping using the KOMPSAT-2 stereo imagery. For this, digital plotting was conducted based on the stereoscopic images. Also the Digital Elevation Model(DEM) and an ortho-image were generated using digital plotting results. An accuracy of digital plotting, DEM, and ortho-image were evaluated by comparing with the existing data. Consequently, we found that horizontal and vertical error of the modeling results based on the Rational Polynomial Coefficient(RPC) was less than 1.5 meters compared with the Global Positioning System(GPS) survey results. The maximum difference of vertical direction between the plotted results in this study and the existing digital map on the scale of 1/5,000 was more than 5 meters according as the topographical characteristics. Although there were some irregular parallax on the images, we realized that it was possible to interpret and plot at least seventy percent of the layer which was required the digital map on the scale of 1/5,000. Also an accuracy of DEM, which was generated based on the digital plotting, was compared with the existing LiDAR DEM. We found that the ortho-images, which were generated using the extracted DEM in this study, sufficiently satisfied with the requirement of the geometric accuracy for an ortho-image map on the scale of 1/5,000.

• Bulletin of the Korean Chemical Society
• /
• v.30 no.12
• /
• pp.2999-3005
• /
• 2009

Among the more than 120 different types of human papillomavirus (HPV), types 16 and 18 have been known to be high risk agents that cause cervical cancer. We examined, in an immuno-chromatographic analysis, the potential of using the early gene product, E7 protein, as a diagnostic marker of cervical cancer caused by HPV. We developed monoclonal antibodies specific to HPV-16 and 18 E7 proteins that were produced from bacterial cells using gene recombinant technology. For each E7 protein, the optimal antibody pair was selected using the immuno-chromatographic sandwichtype binding system based on the lateral flow through membrane pores. Under these conditions, this rapid testing assay had a detection capability as low as 2 ng/mL of E7 protein. Furthermore, since viral analysis required the host cell to be lysed using chemicals such as detergents, it was possible that the E7 protein was structurally damaged during this process, which would result in a decrease in detection sensitivity. Therefore, we examined the detrimental effects caused by different detergents on the E7 protein using HeLa cells as the host. In these experiments, we found that the damage caused by the detergent, nonylphenylpolyethylene glycol (NP-40), was minimal relative to Triton X-100 commonly used for the cell lysis. Temperature also affected the stability of the E7 protein, and we found that the E7 protein was stabilized at 4$^{\circ}C$ for about 2 h, which was 4 times longer than at room temperature. Finally, a HPV-infected cervical cancer cell line, which was used as a real sample model, was treated using the optimized conditions and the presence of E7 proteins were analyzed by immuno-chromatography. The results of this experiment demonstrated that this rapid test could specifically detect HPV-infected samples.

• Bulletin of the Korean Chemical Society
• /
• v.30 no.12
• /
• pp.2993-2998
• /
• 2009

An enzyme-linked immunosorbent assay (ELISA)-on-a-chip (EOC) biosensor combined with cell concentration technology based on immuno-magnetic separation (IMS) was investigated for use as a potential tool for early screening of Listeria monocytogenes (L. monocytogenes) in food products. The target analyte is a well-known pathogenic foodborne microorganism and outbreaks of the food poisoning typically occur due to contamination of normal food products. Thus, the aim of this study was to develop a rapid and reliable sensor that could be utilized on a daily basis to test food products for the presence of this pathogenic microorganism. The sensor was optimized to provide a high detection capability (e.g., 5.9 ${\times}\;10^3$ cells/mL) and, to eventually minimize cultivation time. The cell density was condensed using IMS prior to analysis. Since the concentration rate of IMS was greater than 100-fold, this combination resulted in a detection limit of 54 cells/mL. The EOC-IMS coupled analytical system was then applied to a real sample test of fish intestines. The system was able to detect L. monocytogenes at a concentration of 2.4 CFU/g after pre-enrichment for 6 h from the onset of cell cultivation. This may allow us to monitor the target analyte at a concentration less than 1 CFU/g within a 9 h-cultivation provided a doubling time of 40 min is typically maintained. Based on this estimation, the EOC-IMS system can screen and detect the presence of this microorganism in food products almost within working hours.

• Korean Journal of Child Education & Care
• /
• v.17 no.2
• /
• pp.181-203
• /
• 2017

The purpose of this research is to reveal the difference of teacher-child interaction in relation to professionalism awareness and sensitivity level of early childhood teachers. To this end, we used professionalism awareness, sensitivity, teacher-child interaction as parameters for 226 early childhood teachers in Jeonbuk province. Here are the results : First, we investigated the difference of professionalism awareness, sensitivity, teacher-child interaction in relation to the background of early childhood teachers, and it differs by the age, career, educational level of the teachers. Second, we made group comparison to see the difference of teacher-child interaction depending on professionalism awareness level of teachers, and teachers with high professionalism awareness showed meaningfully higher emotional, verbal, behavioral interaction level than lower group. Third, we made group comparison to see the difference of teacher-child interaction depending on sensitivity level, and teachers of high sensitivity shows meaningfully higher emotional, verbal interaction than lower group. This result could be potential data to ameliorate the quality of teacher-child interaction, and it implies that the improvement of professionalism and sensitivity level could contribute to professional performance capability of teachers.

• Spatial Information Research
• /
• v.22 no.3
• /
• pp.47-58
• /
• 2014

The credible measurement, reporting and verification (MRV) is among the most critical elements in UN-REDD (United Nations programme on Reducing Emissions from Deforestation and forest Degradation in Developing Countries). This study is intended to explore MRV potential in terms of UN-REDD opportunity cost estimation using satellite image for Mt. Geum-gang of North Korea. A visual interpretation were conducted to evaluate MRV conditions by sub-dividing or decomposing the images with different pixel size into a three types of hierarchical tree structure that helps dealing with spatial variability within each subarea. The permanent record of standard satellite remote sensing system demonstrated its capability of presenting area-wide visual evidences of MRV conditions in Mt. Geum-gang (such as the identification of forested area, degradation trends for forest space, three types of hierarchical land-cover and land use tree structure, carbon density in the landscape). Satellite data could be accepted as legally binding proof when it comes to REDD opportunity cost estimation since several cases exist where remote sensing has been used as legal evidence in ICJ (International Court of Justice) and UN resolution. It doesn't seem very difficult to comply with MRV requirements for UN-REDD opportunity cost calculation due to the probative value of satellite data. It is anticipated that this research output could be used as a valuable reference for Korea-based enterprises exploring REDD project sites and the carbon traders to ensure MRV potentials using satellite image in UN-REDD Opportunity Cost estimation.

• Structural Engineering and Mechanics
• /
• v.31 no.2
• /
• pp.181-210
• /
• 2009

This paper presents the theoretical developments of an exact finite strip for the buckling and initial post-buckling analyses of isotropic flat plates. The so-called exact finite strip is assumed to be simply supported out-of-plane at the loaded ends. The strip is developed based on the concept that it is effectively a plate. The present method, which is designated by the name Full-analytical Finite Strip Method in this paper, provides an efficient and extremely accurate buckling solution. In the development process, the Von-Karman's equilibrium equation is solved exactly to obtain the buckling loads and the corresponding form of out-of-plane buckling deflection modes. The investigation of thin flat plate buckling behavior is then extended to an initial post-buckling study with the assumption that the deflected form immediately after the buckling is the same as that obtained for the buckling. It is noted that in the present method, only one of the calculated out-of-plane buckling deflection modes, corresponding to the lowest buckling load, i.e., the first mode is used for the initial post-buckling study. Thus, the postbuckling study is effectively a single-term analysis, which is attempted by utilizing the so-called semi-energy method. In this method, the Von-Karman's compatibility equation governing the behavior of isotropic flat plates is used together with a consideration of the total strain energy of the plate. Through the solution of the compatibility equation, the in-plane displacement functions which are themselves related to the Airy stress function are developed in terms of the unknown coefficient in the assumed out-of-plane deflection function. These in-plane and out-of-plane deflected functions are then substituted in the total strain energy expressions and the theorem of minimum total potential energy is applied to solve for the unknown coefficient. The developed method is subsequently applied to analyze the initial postbuckling behavior of some representative thin flat plates for which the results are also obtained through the application of a semi-analytical finite strip method. Through the comparison of the results and the appropriate discussion, the knowledge of the level of capability of the developed method is significantly promoted.