• Journal of Microbiology and Biotechnology
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• 제32권3호
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• pp.307-316
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• 2022

Yeasts play an important role in spontaneous fermentation of traditional alcoholic beverages. Our previous study revealed that a mixed-consortia of both Saccharomyces and non-Saccharomyces yeasts were responsible for fermentation of khadi, a popular, non-standardized traditional beverage with an immense potential for commercialization in Botswana. Functional characterization of isolated fermenting yeasts from mixed consortia is an indispensable step towards the selection of potential starter cultures for commercialization of khadi. In this study, we report the characterization of 13 khadi isolates for the presence of brewing-relevant phenotypes such as their fermentative capacity, ability to utilize a range of carbon sources and their ability to withstand brewing-associated stresses, as a principal step towards selection of starter cultures. Khadi isolates such as Saccharomyces cerevisiae, Saccharomycodes ludwigii and Candida ethanolica showed good brewing credentials but Lachancea fermentati emerged as the isolate with the best brewing attributes with a potential as a starter culture. However, we were then prompted to investigate the potential of L. fermentati to influence the fruity aromatic flavor, characteristic of khadi. The aroma components of 18 khadi samples were extracted using headspace solid phase micro-extraction (HS-SPME) and identified using a GC-MS. We detected esters as the majority of volatile compounds in khadi, typical of the aromatic signature of both khadi and L. fermentati associated fermentations. This work shows that L. fermentati has potential for commercial production of khadi.

• 한국마이크로전자및패키징학회:학술대회논문집
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• 한국마이크로전자및패키징학회 2001년도 추계 기술심포지움
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• pp.55-60
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• 2001

RF MEMS is an exciting emerging technology that has great potential to develop TOC (Transceiver-on-Chip). Applications of the RF MEMS to wireless communications systems are presented. The ability of the RF MEMS technology to enhance the performance and to reduce the size of passive components, in particular, switches, inductors, and tunable capacitors, is addressed. A number of potential wireless system opportunities for the TOC are awaiting the maturation of the RF MEMS technology.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.699-701
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• 2002

The current study demonstrates the ability of the marine cyanobacterium Oscillatoria willei BDU130511 to disinfect raw sewage. Within a holding time of 3 h under laboratory conditions, the organism drastically reduced in the total bacterial and coliform counts at various pH levels, in both unbuffered and buffered sewage, thereby suggesting a potential role for cyanobacteria in wastewater treatment.

• Preventive Nutrition and Food Science
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• 제22권2호
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• pp.107-117
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• 2017

The purpose of this study was to evaluate the antioxidant and anticholinesterase activities of yellow and white bitter yams from South Western Nigeria using methanolic extraction and simulated gastrointestinal digestion models. The phenolic compounds in the bitter yam varieties were evaluated by high performance liquid chromatography with a diode array detector (HPLC-DAD). The total phenolic content of the bitter yams was measured by the Folin-Ciocalteu method, reductive potential by assessing the ability of the bitter yam to reduce $FeCl_3$ solution, and the antioxidant activities were determined by the 2,2-diphenyl-1-picrylhydrazyl radical ($DPPH^{\cdot}$) scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation ($ABTS^{{\cdot}+}$) scavenging activity, nitric oxide radical ($NO^{\cdot}$) scavenging ability, hydroxyl radical scavenging ability, and ability to inhibit $Fe^{2+}$-induced lipid oxidation. The HPLC-DAD analysis revealed the presence of some phenolic compounds in the studied bitter yam varieties, with varying degree of quantitative changes after cooking. The antioxidant indices (total phenolic content, total flavonoid content, reducing power, $DPPH^{\cdot}$ scavenging activity, $ABTS^{{\cdot}+}$ scavenging activity, and $NO^{\cdot}$ scavenging activity) were higher in the simulated gastrointestinal digestion model compared to the methanolic extract, with the in vitro digested cooked white bitter yam ranking higher. Similarly, the in vitro digested yams had a higher inhibitory action against lipid oxidation compared to the methanolic extracts, with the cooked white bitter yam ranking high. The methanolic extracts and in vitro enzyme digests showed no acetylcholinesterase inhibitory abilities, while methanolic extracts and the in vitro enzyme digest displayed some level of butyrylcholinesterase inhibitory activities. Therefore the studied bitter yams could be considered as possible health supplements.

• Molecules and Cells
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• 제45권12호
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• pp.935-949
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• 2022

Liver cancer has a high prevalence, with majority of the cases presenting as hepatocellular carcinoma (HCC). The prognosis of metastatic HCC has hardly improved over the past decade, highlighting the necessity for liver cancer research. Studies have reported the ability of the KiSS1 gene to inhibit the growth or metastasis of liver cancer, but contradictory research results are also emerging. We, therefore, sought to investigate the effects of KiSS1 on growth and migration in human HCC cells. HepG2 human HCC cells were infected with lentivirus particles containing KiSS1. The overexpression of KiSS1 resulted in an increased proliferation rate of HCC cells. Quantitative polymerase chain reaction and immunoblotting revealed increased Akt activity, and downregulation of the G1/S phase cell cycle inhibitors. A significant increase in tumor spheroid formation with upregulation of β-catenin and CD133 was also observed. KiSS1 overexpression promoted the migratory, invasive ability, and metastatic capacity of the hepatocarcinoma cell line, and these effects were associated with changes in the expressions of epithelial mesenchymal transition (EMT)- related genes such as E-cadherin, N-cadherin, and slug. KiSS1 overexpression also resulted in dramatically increased tumor growth in the xenograft mouse model, and upregulation of proliferating cell nuclear antigen (PCNA) and Ki-67 in the HCC tumors. Furthermore, KiSS1 increased the angiogenic capacity by upregulation of the vascular endothelial growth factor A (VEGF-A) and CD31. Based on these observations, we infer that KiSS1 not only induces HCC proliferation, but also increases the metastatic potential by increasing the migratory ability and angiogenic capacity.

• 대한수학교육학회지:수학교육학연구
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• 제11권2호
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• pp.341-349
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• 2001

Infinity is one of the important concept in mathematics, science, philosophy etc. In history of mathematics, potential infinity concept conflicts with actual infinity concept. Reason that mathematicians refuse actual infinity concept during long period is because that actual infinity concept causes difficulty in our perceptions. This phenomenon is called epistemological obstacle by Brousseau. Potential infinity concept causes difficulty like history of development of infinity concept in mathematics learning. Even though students team about actual infinity concept, they use potential infinity concept in problem solving process. Therefore, we must make clear epistemological obstacles of infinity concept and must overcome them in learning of infinity concept. For this, it is useful to experience visualization about infinity concept. Also, it is to develop meta-cognition ability that students analyze and control their problem solving process. Conclusively, students must adjust potential infinity concept, and understand actual infinity concept that is defined in formal mathematics system.

• 한국융합학회논문지
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• 제10권12호
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• pp.7-15
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• 2019

대학에서는 취업에 대한 부담을 완화시키고, 경제발전을 촉진할 수 있는 혁신창업인재를 양성하기 위하여 대학생의 창업교육을 중시하고 있다. 중국은 "대중창업, 만인혁신"을 배경으로 대학생들에게 기업가 정신교육에 큰 비중을 두고 있지만, 실제 창업 성공률이 높지 않은 것은 창업능력의 부족에 기인한 것이다. 이에 본 논문은 중국 길림대학에서 창업한 대학생들을 탐방대상으로 선정하였고, 이들에 대한 심층면접을 통하여 얻은 자료를 질적 연구방법인 귀납적 토대이론에 근거하여, 대학생들의 창업능력의 함축성에 대한 요소분석을 하였다. 이를 통하여 창업능력의 개념모형을 혁신적으로 제시하였다. 연구결과로는 대학생의 창업능력에는 창업 추진력, 관리능력, 창업 재능과 잠재 능력으로 구성되어 있는 것으로 나타났다. 본 논문은 대학에서 창업 성공률을 높일 수 있는 창업교육을 실시하는데 지침적 의미가 있는 연구이다.

• 농촌계획
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• 제23권3호
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• pp.21-36
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• 2017

Climate change is intensifying storms and floods around the world. Where nature has been destroyed by development, communities are at risk from these intensified climate patterns. This study was to suggest a methodology for estimating flood vulnerability using Potential Flood Damage(PFD) concept and classify city/county about Potential Flood Damage(PFD) using various typology techniques. To evaluate the PFD at a spatial resolutions of city/county units, the 20 representative evaluation indexing factors were carefully selected for the three categories such as damage target(FDT), damage potential(FDP) and prevention ability(FPA). The three flood vulnerability indices of FDT, FDP and FPA were applied for the 167 cities and counties in Korea for the pattern classification of potential flood damage. Potential Flood Damage(PFD) was classified by using grouping analysis, decision tree analysis, and cluster analysis, and characteristics of each type were analyzed. It is expected that the suggested PFD can be utilized as the useful flood vulnerability index for more rational and practical risk management plans against flood damage.

• International Journal of Computer Science & Network Security
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• 제22권4호
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• pp.408-416
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• 2022

The new millennium is characterized by an unprecedented breakthrough in knowledge and information and communication technologies, and the challenges of the XXI century require modernized paradigms of interaction in all spheres of life. Education continues to play a key role in national and global growth. The key role of education and its leadership in developing creative potential, as the main paradigm of the countries' stability, have significantly influenced educational centers. The developers of educational programs use information technologies as an incentive to develop creative potential of educational process. Professional training of the educational candidate is enhanced by the use of information technologies, so the educational applicants should develop technological skills to be productive members of society. Using the latest achievements in the field of information technologies for the organization of the educational process helps to form the operational style of education applicants' thinking, which provides the ability to acquire skills of processing information, that is presented in the text, graphic, tabular form, and increase the level of general and informational culture necessary for better orientation in the modern information space. The purpose of the research is to determine the effectiveness of information technologies as an incentive to develop creative potential of educational process on the basis of the survey, to establish advantages and ability to provide high-quality education in the context of using information technologies. Methods of research: comparative analysis; systematization; generalization, survey. Results. Based on the survey conducted among students and teachers, it has been found out that the teachers use the following information technologies for the development of creative potential of the educational process: to provide video and audio communication process (100%), Moodle (95,6%), Duolingo (89,7%), LinguaLeo (89%), Google Forms (88%) and Adobe Captivate Prime (80,6%). It is determined that modular digital learning environments (97,9%), interactive exercises tools (96,3%), ICT for video and audio communication (96%) and interactive exercises tools (95,1%) are most conducive to the development of creative potential of the educational process. As a result of the research, it was revealed that implementation of information technologies for the development of creative potential of educational process in educational institutions is a complex process due to a large number of variables, which should be taken into account both on the educational course and on the individual level. It has been determined that the using the model of implementation information technologies for the development of creative potential in educational process, which is stimulated due to this model, benefits both students and teachers by establishing a reliable bilateral connection between teacher and education applicant.

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1068-1077
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• 2006

Pseudomonas sp. PhCN strain, which has the potential to utilize phenol and cyanide as a sole carbon and nitrogen source, was isolated. A comparison of the effect of cyanide on phenol degradation and vice versa by strain PhCN showed that the degradation time was significantly delayed by an increase in either phenol or cyanide concentration, and the greatest activities were obtained in basal medium containing a low concentration of cyanide and phenol. This strain contained two plasmids of approximately 120 kb (pPhCN-1) and 110 kb (pPhCN-2). Plasmid curing experiments produced a plasmid-free strain as well as strains containing either the 120- or the 110 kb plasmid. The strains were tested for their ability to utilize phenol and KCN. The results demonstrated that the ability to utilize phenol was encoded by the 120 kb plasmid, whereas the ability to utilize cyanide appeared to be encoded by the chromosome.