• 제목/요약/키워드: Potential Shock

검색결과 289건 처리시간 0.027초

Transient thermo-piezo-elastic responses of a functionally graded piezoelectric plate under thermal shock

  • Xiong, Qi-lin;Tian, Xin
    • Steel and Composite Structures
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    • 제25권2호
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    • pp.187-196
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    • 2017
  • In this work, transient thermo-piezo-elastic responses of an infinite functionally graded piezoelectric (FGPE) plate whose upper surface suffers time-dependent thermal shock are investigated in the context of different thermo-piezo-elastic theories. The thermal and mechanical properties of functionally graded piezoelectric plate under consideration are expressed as power functions of plate thickness variable. The solution of problem is obtained by solving the corresponding finite element governing equations in time domain directly. Transient thermo-piezo-elastic responses of the FGPE plate, including temperature, stress, displacement, electric intensity and electric potential are presented graphically and analyzed carefully to show multi-field coupling behaviors between them. In addition, the effects of functionally graded parameters on transient thermo-piezo-elastic responses are also investigated to provide a theoretical basis for the application of the FGPE materials.

Expression and Characterization of Recombinant E2 Protein of Hepatitis C Virus by Insect Cell/Baculovirus Expression System

  • Han, Bong-Kwan;Lee, Bum-Yong;Min, Mi-Kyung;Jung, Kyung-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제8권4호
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    • pp.361-368
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    • 1998
  • The E2 protein of HCV (hepatitis C virus) is thought to have a potential role in the development of subunit vaccines and diagnostics. To express it by the insect cell/baculovirus expression (Bacu) system, we constructed a recombinant Autographa californica nuclear polyhedrosis virus (AcIL3E2), determined the most appropriate expression conditions in terms of host cell line and culture medium, and characterized the expressed HCV E2 protein. A culture system using Trichoplusia ni BTI-TN5Bl-4 cells and SF 900IISFM medium expressed a relatively high level of HCV E2 protein. It was revealed that its glycosylation properties and subcellular localization were almost the same as the ones in the mammalian cell expression system previously reported, suggesting the recombinant HCV E2 protein derived from our Bacu system can be utilized for development of a subunit vaccine and diagnostics. Interestingly, HCV E2 protein was not degraded at all even at 43 h post-heat shock in the heat shock-induced necrotic cells, probably due to its integration into the microsomal membrane, indicating that heat shock can be employed to purify HCV E2 protein.

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충격압분공정으로 제조된 나노 니켈/알루미늄 혼합분말재의 특성 평가 (Evaluation of the Reactivity of Bulk Nano Ni/Al Powder Manufactured by Shock Compaction Process)

  • 김우열;안동현;박이주;김형섭
    • 소성∙가공
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    • 제26권4호
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    • pp.216-221
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    • 2017
  • Recently, interest in multifunctional energetic structural materials (MESMs) has grown due to their multifunctional potential, especially in military applications. However, there are few studies about extrinsic factors that govern the reactivity of MESMs. In this paper, a shock compaction process was performed on the nano Ni/Al-mixed powder to investigate the effect of particle size on the shock reaction condition. Additionally, heating the statically compacted specimen was also performed to compare the mechanical properties and microstructure between reacted and unreacted material. The results show that the agglomerated structure of nanopowders interrupts the reaction by reducing the elemental boundary. X-ray diffraction analysis shows that the NiAl and $Ni_3Al$ intermetallics are formed on the reacted specimen. The microhardness results show that the $Ni_3Al$ phase has a higher hardness than NiAl, but the portion of $Ni_3Al$ in the reacted specimen is minor. In conclusion, using Ni/Al composites as a reactive material should focus on energetic use.

Ethanol Extract of Ulmus pumila Ameliorates Heat Stress through the Induction of Heat Shock Proteins Expression in RAW264.7 Macrophage Cells

  • dela Cruz, Joseph;Byambaragchaa, Munkhzaya;Choi, Seok-Geun;Hwang, Seong-Gu
    • 한국축산시설환경학회지
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    • 제20권4호
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    • pp.147-154
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    • 2014
  • Heat stress is a significant burden to animal production in most areas of the world. Improving our knowledge of physiological and metabolic mechanisms of acclimation may contribute to the development of procedures that may help to maintain health and production efficiency under hot temperature. The effect of Ulmus pumila (UP) extract in inducing Heat Shock Proteins (HSPs) expression in heat-stressed RAW264.7 macrophage cells was investigated. Cell viability assay showed a dose dependent increase in cells after treatment with UP for 24 hours. RT-PCR and western blot analysis showed that increasing concentrations of UP induce the expression of Heat Shock Factor 1 (HSF1) and dose dependently upregulated the expression of Heat shock protein 70 (Hsp70) and Hsp90. LPS-induced nitric oxide was dose-dependently reduced while phagocytic activity greatly recovered with UP treatment. These data demonstrated that UP can be a potential candidate in the development of cytoprotective agent against heat stress.

Differential expression of heat shock protein 90, 70, 60 in chicken muscles postmortem and its relationship with meat quality

  • Zhang, Muhan;Wang, Daoying;Geng, Zhiming;Sun, Chong;Bian, Huan;Xu, Weimin;Zhu, Yongzhi;Li, Pengpeng
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권1호
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    • pp.94-99
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    • 2017
  • Objective: The aim of this study was to investigate the expression of heat shock protein (HSP) 90, 70, and 60 in chicken muscles and their possible relationship with quality traits of meat. Methods: The breast muscles from one hundred broiler chickens were analyzed for drip loss and other quality parameters, and the levels of heat shock protein (HSP) 90, 70, and 60 were determined by immunoblots. Results: Based on the data, chicken breast muscles were segregated into low (drip loss${\leq}5%$), intermediate (5%${\geq}9.5$) drip loss groups. The expression of HSP90 and HSP60 were significantly lower in the high drip loss group compared to that in the low and intermediate drip loss group (p<0.05), while HSP70 was equivalent in abundance in all groups (p>0.05). Conclusion: Results of this study suggests that higher levels of HSP90 and HSP60 may be advantageous for maintenance of cell function and reduction of water loss, and they could act as potential indicator for better water holding capacity of meat.

침수조건에 따른 저압 지중함의 감전 위험성 평가 (The Assesment of Electric Shock Rate of Low Voltage Joint-Box Based Submerged Condition)

  • 심건보;김경철;김한상;김종민
    • 한국조명전기설비학회:학술대회논문집
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    • 한국조명전기설비학회 2007년도 춘계학술대회 논문집
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    • pp.265-270
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    • 2007
  • The potential and step voltage distribution around low voltage joint-box cover were simulated with the variation of resistivity of water, depth of submerged water and point of leakage current. The potential distribution is very high gradient around low voltage joint-box, this condition is very dangerous states.

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ASYMPTOTIC SOLUTIONS OF HYDRODYNAMIC INTERFACIAL INSTABILITIES IN CYLINDRICAL FLOW

  • Sohn, Sung-Ik
    • 한국수학교육학회지시리즈B:순수및응용수학
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    • 제20권4호
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    • pp.259-267
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    • 2013
  • We present a high-order potential flow model for the motion of hydrodynamic unstable interfaces in cylindrical geometry. The asymptotic solutions of the bubbles in the gravity-induced instability and the shock-induced instability are obtained from the high-order model. We show that the model gives significant high-order corrections for the solution of the bubble.

Risk Management 관점에서 신재생에너지 투자 (Risk Management and Governmental Investment on New & Renewable Energy)

  • 안은영
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2006년도 춘계학술대회
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    • pp.360-363
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    • 2006
  • Regester & Larkin(2005) suggest the issue and risk lifecycle analysis method in risk management, made up of the potential, emerging, current, crisis, and dormant stages. Investment on New & Renewable Energy is meaningful in effect ive risk management for diminishing or reducing the shock of a energy current, at the potential stage. In this study, we survey the risk of traditional fossil fuel projects and develop the risk analysis model for new & renewable energy projects specially geothermal energy resources and gas-hydrate resources.

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Heat Shock Protein Augmentation of Angelica gigas Nakai Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes

  • Lumbera, Wenchie Marie L.;Cruz, Joseph dela;Yang, Seung-Hak;Hwang, Seong Gu
    • Asian-Australasian Journal of Animal Sciences
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    • 제29권3호
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    • pp.419-427
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    • 2016
  • There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted adipogenic differentiation in murine 3T3-L1 preadipocytes under the normal temperature condition. However, its effect in heat shocked 3T3-L1 cells has not been established. In this study, we investigated the effect of AGN root hot water extract in the adipogenic differentiation of murine 3T3-L1 preadipocytes following heat shock and its possible mechanism of action. Thermal stress procedure was executed within the same stage of preadipocyte confluence (G0) through incubation at $42^{\circ}C$ for one hour and then allowed to recover at normal incubation temperature of $37^{\circ}C$ for another hour before AGN treatment for both cell viability assay and Oil Red O. Cell viability assay showed that AGN was able to dose dependently (0 to $400{\mu}g/mL$) increase cell proliferation under normal incubation temperature and also was able to prevent cytotoxicity due to heat shock accompanied by cell proliferation. Confluent preadipocytes were subjected into heat shock procedure, recovery and then AGN treatment prior to stimulation with the differentiation solution. Heat shocked preadipocytes exhibited reduced differentiation as supported by decreased amount of lipid accumulation in Oil Red O staining and triglyceride measurement. However, those heat shocked preadipocytes that then were given AGN extract showed a dose dependent increase in lipid accumulation as shown by both evaluation procedures. In line with these results, real-time polymerase chain reaction (RT-PCR) and Western blot analysis showed that AGN increased adipogenic differentiation by upregulating heat shock protection related genes and proteins together with the adipogenic markers. These findings imply the potential of AGN in heat shock amelioration among 3T3-L1 preadipocytes through heat shock factor and proteins augmentation and enhanced adipogenic marker expression.

전원설비에 의한 공간조건별 전위분포 사례 연구 (Case Studies for Potential Distribution due to Power Source by Environmental Condition)

  • 정진수;정종욱;김선구
    • 조명전기설비학회논문지
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    • 제25권1호
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    • pp.50-58
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    • 2011
  • In this paper, potential distribution analysis through the six kinds of case studies examined in the environmental conditions change within a limited space, by the power supply system installed. To interpret the potential distribution using electromagnetic field analysis program and the parameters were state of the ground, the outer wall of the configuration state and the presence of human potential distribution was simulated. Potential distribution analysis, the size of nominal voltage, depending on the impedance of human body to be determined on the impact of surprise decision, but if equipped with appropriate protective equipment can be protected from lightning have been confirmed.