• 제목/요약/키워드: Potential Shock

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Transduced HSP27 protein protects neuronal cell death by enhancing FALS-associated SOD1 mutant activity

  • An, Jae-Jin;Lee, Yeom-Pyo;Kim, Dae-Won;Sohn, Eun-Joung;Jeong, Hoon-Jae;Kang, Hye-Won;Shin, Min-Jae;Kim, Mi-Jin;Ahn, Eun-Hee;Jang, Sang-Ho;Kang, Jung-Hoon;Kang, Tae-Cheon;Won, Moo-Ho;Kwon, Oh-Shin;Cho, Sung-Woo;Lee, Kil-Soo;Park, Jin-Seu;Eum, Won-Sik;Choi, Soo-Young
    • BMB Reports
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    • v.42 no.3
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    • pp.136-141
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    • 2009
  • Familial Amyotrophic lateral sclerosis (FALS) is a progressive neurodegenetative disorder induced by mutations of the SOD1 gene. Heat shock protein 27 (HSP27) is well-defined as a stress-inducible protein, however the its role in ALS protection has not yet been established. To investigate the role HSP27 may have in SOD1 mutant-mediated apoptosis, human SOD1 or HSP27 genes were fused with a PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame fusion protein, which was then transduced into cells. We found the purified PEP-1-HSP27 fusion proteins can be transduced efficiently into neuronal cells and protect against cell death by enhancing mutant SOD1 activity. Moreover, transduced PEP-1-HSP27 efficiently prevents protein aggregation produced by oxidative stress. These results suggest that transduced HSP27 fusion protein may be explored as a potential therapeutic agent for FALS patients.

Water Extract of Rhei Rhizoma Prevent Production of Reactive Oxygen Species and Loss of Mitochondrial Membrane Potential in a Hypoxia Model of Cultured Neurons (배양 신경세포의 저산소증모델에서 대황 물추출액의 항산화 및 사립체막전위 소실 억제 효능)

  • Lee, Hyun-Sook;Moon, Il-Soo
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1631-1636
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    • 2008
  • Rhei Rhizoma (RR; 大黃) consists of the underground parts (rhizome and root) of Rheum officinale Baill. and Rheum palmatum L. (Polygonaceae), and is widely used in Southeast Asian folk medicine to alleviate liver and kidney damages. In this study, we investigated into the efficacy and mechanism of RR water extract in supporting neuronal survival in a hypoxia model of cultured rat hippocampal neurons. RR exhibited no cytotoxicity up to 10 ${\mu}g$/ml and exhibited neurosupportive effects at 2.5 ${\mu}g$/ml in normoxia. When RR was added to the culture media on 10 days in vitro (DIV10) and given a hypoxic shock (2% $O_2$/5% $CO_2$, 3 hr, $37^{\circ}C$) on DIV13, RR exhibited neuroprotective effects on 5 days post-shock. $H_2DCF$ stainings indicated that RR effectively prevents ROS production in both normoxia and hypoxia. JC-1 stainings showed that RR prevents dissipation of MMP in hypoxia. These results indicate that RR protects neurons by suppressing ROS production and MMP loss.

The National Survey of Acute Pulmonary Thromboembolism in Korea (급성 폐혈전색전증 전국 실태 조사 보고)

  • Scientific Committee for National Survey of Acute Pulmonary Thromboembolism, Korean Academy of Tuberculosis and Respiratory Diseases
    • Tuberculosis and Respiratory Diseases
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    • v.54 no.1
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    • pp.5-14
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    • 2003
  • Background : According to the study in ICOPER (International Cooperative Pulmonary Embolism Registry), the overall mortality rate of acute pulmonary thromboembolism (APTE) at 3 months is 17.4%. According to the study for current status of APTE in Japan, the hospital mortality rate is 14%. Although the incidence and mortality rate of APTE has been increasing, patient characteristics, management strategies, and outcome of APTE in the Korean population have not yet been assessed in large series. We therefore performed the national survey for the current status of APTE in the Korean population. Methods : 808 registry patients with APTE were analyzed with respect to clinical characteristics, risk factors, diagnostic procedures, treatment, and clinical outcome. Results : Main risk factors were immobilization, recent major surgery, and cancer. Common symptoms were dyspnea and chest pain. Common signs were tachypnea and tachycardia. The majority of registry patients underwent lung perfusion scanning. Spiral CT was used in 309 patients(42.9%), and angiography in 48 patients(7.9%). Heparin was the most widely used treatment. On multivariate logistic regression analysis, onset in hospital (odds ratio 1.88, p=0.0385), lung cancer (odds ratio 9.20, p=0.0050), tachypnea (odds ratio 3.50, p=0.0001), shock (odds ratio 6.74, p=0.0001), and cyanosis (odds ratio 3.45, p=0.0153) were identified as significant prognostic factors. The overall mortality rate was 16.9% and mortality associated with APTE was 9.0%. Conclusions : The present registry demonstrated the clinical characteristics, diagnostic strategies, management and outcome of patient with APTE in Korea. The mortality rate was 9.0%, and the predictors of mortality were onset in hospital, lung cancer, tachypnea, shock, and cyanosis. These results may be important for risk stratification as well as for the identification of potential candidates for more aggressive treatment.

A Study on the Safety Grounding for Prevention of Electric Shock Hazard in Construction of Industrial Plant in Maritime Landfill Area (해상 매립 지역 산업 플랜트 건설 시 감전 재해 예방을 위한 안전 접지에 관한 연구)

  • Kim, Hong-Yong;Jang, Ung-Burm
    • Journal of the Society of Disaster Information
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    • v.13 no.3
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    • pp.305-312
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    • 2017
  • In our society, the advanced, advanced, and information industries have continued to grow and now live in the era of the fourth industrial revolution. As the industry develops, the load of the users has also increased so much that it is deepened by the energy shortage phenomenon and the construction of additional energy facilities is required. Therefore, energy plant construction work is being actively carried out in the coastal area. In particular, it is common to build a plant in the ground by filling the coast with soil in other regions, reflecting the fact that Korea is lacking in the country when constructing power plants, gas and petrochemical plants. Current domestic grounding designs are designed or constructed to suit only the use of grounding resistors based on the electrical equipment design technical standards. However, in the case of a plant facility constructed in the untested buried soil, when the lightning current and the abnormal current are inputted, the facility operator or the user due to the elevation of the ground potential is seriously exposed to the risk of electric shock disaster. In this paper, we analyze the ground resistivity of the landfilled soil and use a computer program (CDEGS) based on KS C IEC 61936-1, We analyze the contact voltage and stratification voltage and propose a grounding design optimized for plant installation.

Prokaryotic Selectivity, Anti-endotoxic Activity and Protease Stability of Diastereomeric and Enantiomeric Analogs of Human Antimicrobial Peptide LL-37

  • Nan, Yong-Hai;Lee, Bong-Ju;Shin, Song-Yub
    • Bulletin of the Korean Chemical Society
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    • v.33 no.9
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    • pp.2883-2889
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    • 2012
  • LL-37 is the only antimicrobial peptide (AMP) of the human cathelicidin family. In addition to potent antimicrobial activity, LL-37 is known to have the potential to inhibit lipolysaccharide (LPS)-induced endotoxic effects. To provide the stability to proteolytic digestion and increase prokaryotic selectivity and/or anti-endotoxic activity of two Lys/Trp-substituted 19-meric antimicrobial peptides (a4-W1 and a4-W2) designed from IG-19 (residues 13-31 of LL-37), we synthesized the diastereomeric peptides (a4-W1-D and a4-W2-D) with D-amino acid substitution at positions 3, 7, 10, 13 and 17 of a4-W1 and a4-W2, respectively and the enantiomeric peptides (a4-W1-E and a4-W2-E) composed D-amino acids. The diastereomeric peptides exhibited the best prokaryotic selectivity and effective protease stability, but no or less anti-endotoxic activity. In contrast, the enantiomeric peptides had not only prokaryotic selectivity and anti-endotoxic activity but also protease stability. Our results suggest that the hydrophobicity and ${\alpha}$-helicity of the peptide is important for anti-endotoxic activity. In particular, the enantiomeric peptides showed potent anti-endotoxic and LPS-neutralizing activities comparable to that of LL-37. Taken together, both a4-W1-E and a4-W2-E holds promise as a template for the development of peptide antibiotics for the treatment of endotoxic shock and sepsis.

Elastic Modulus Measurement of a Large Size Digital TV Display Unit (대형 Digital TV용 Display Unit의 강성 측정)

  • Kim Chang-Hoi;Moon Seong-In;Choi Jae-Boons;Kim Young-Jin;Lee Jeoung-Gwen;Koo Ja-Choon
    • Journal of the Korean Society for Precision Engineering
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    • v.22 no.3 s.168
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    • pp.115-122
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    • 2005
  • As the digital TV markets rapidly growing, many manufacturers introduce large size flat screen TV units. There are two different display types available to large size models which are plasma and TFT-LCD. Since both are constructed with thin large panels that are mostly fragile to even moderate mechanical shock inputs. Some large size panels are severely resonated by the acoustic sound generated TV which deteriorates video quality. Recognizing the potential problems of large displays, accurate measurement of the panels is to be an essential task for the reliable design. Measurement of mechanical properties of a thin large crystallized panel such as TFT-LCD display with traditional material testing equipments is challenging. Since TFT-LCDs are constructed with combination of brittle glass panels, polymer sheets, and liquid crystal, their properties are not only anisotropic but also usually non-linear. Accurate measurement of the properties often requires very expensive facilities. Especially when the size of the test sample is as large as 40-inch or wider, direct measurement cost is prohibitive. Even worse, machining of the large TFT-LCD to make a smaller size specimen that could be fit into a material tester is not possible because of liquid crystal leakage. A new method fer the measurement of elastic modulus of large TFT-LCD panel is presented in this article. The suggested method provides a simple, economic, and user-friendly way fer measuring the elastic modulus of large panels with considerable level of accuracy.

Simulation Experiments for Ubiquitous Traffic Flow Management (유비쿼터스 환경에서 최적교통관리를 위한 시뮬레이션 평가)

  • Park, Eun-Mi;Go, Myeong-Seok
    • Journal of Korean Society of Transportation
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    • v.27 no.3
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    • pp.71-77
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    • 2009
  • The ubiquitous transportation system environments make it possible to collect each vehicle's position and velocity data and to perform more sophisticated traffic flow management at individual vehicle or platoon level through V2V and V2I communications. The VISSIM simulation experiments were performed to address the issues in developing the preventive congestion management algorithm proposed in the companion paper. Traffic flow stability measures were developed based on the platoon profile, which enables us to explicitly consider traffic flow stability in traffic flow management. Traffic flow management strategies according to the traffic flow states were proposed: Maintain the equilibrium speed for free flow state, maintain the traffic flow stability by platoon control for critical state, and surpress the shock wave propagation for congested state. And finally potential benefit of the proposed traffic flow management scheme was evaluated based on the simulation experiment results. It is considered that extensive field experiments should be performed to confirm the simulated results.

Improvement in Tribological Properties of Carbon Steel Sintered by Powder Metallurgy (분말 야금에 의해 소결된 강철의 트라이볼로지 특성 향상)

  • Choi, S.I.M.;Karimbaev, R.;Pyun, Y.S.;Amanov, A.
    • Tribology and Lubricants
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    • v.36 no.4
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    • pp.244-252
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    • 2020
  • Materials manufactured by powder metallurgy (PM) are widely used in various applications such as water pump, shock absorber, and airplane components due to the reduction in the cost and weight. In this study, tribological properties of carbon steel subjected by surface treatment were investigated. The main purpose is to increase the strength and improve the tribological properties by reducing pores that formed by PM. Moreover, the surface treatment was carried out at room and high temperatures (RT and HT). The surface roughness of the untreated (NON) and treated (AFTER) samples was measured. It was found that the surface roughness was reduced after both the RT AFTER and HT AFTER compared to RT NON sample. The tribological properties of the samples were performed against bearing steel ball under dry conditions. The friction coefficient of the RT NON samples was reduced by 22% and 56% RT AFTER and HT AFTER, respectively. The wear volume of the RT NON sample was also reduced by 43% and 87% RT AFTER and HT AFTER, respectively. Tribocorrosion tests were also performed and it was found that the surface of the RT AFTER, HT AFTER samples was less corroded compared to RT NON sample. The HT AFTER sample demonstrated a relatively higher corrosion potential in comparison with the RT AFTER samples. Hence, it was confirmed that after surface modification the surface roughness and hardness of the samples were significantly improved resulting in improvement in tribological and tribocorrosion behaviors of PM carbon steel.

Anti-allergic effect of Osterici Radix water extract in human mast cells (북강활(北羌活) 물추출물의 항알레르기 효능에 대한 연구)

  • Jung, Jin-Ki;Jung, Hye-Mi;Seo, Woon-Gyo;Park, Yong-Ki
    • The Korea Journal of Herbology
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    • v.25 no.3
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    • pp.35-41
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    • 2010
  • Objectives : Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase of allergic disease including allergic rhinitis (AR). The purpose of this study was to investigate the effects of Osterici Radix, roots of Ostericum koreanum Maximowicz in human mast cells and experimental allergic animal models. Methods : The anti-allergic effect of Osterici Radix water extract (NK-W) was investigated in human mast cell line, HMC-1 cells, and compound 48/80-induced systemic anaphylactic response in rats and ovalbumin (OVA)-induced AR in mice. Animals were orally administrated with NK-W (10 and 50 mg/kg) or anti-histamine drug, dosodium cromoglycate (50 mg/kg), and then intraperitoneally injected with compound 48/80 (8 mg/kg) or sensitized with 0.1% OVA into nasal. Animals were observed plasma histamine and histological changes of nasal mucosa. Also, mast cell degranulation and histamine production were determined in compound 48/80-stimulated HMC-1 cells. Results : NK-W inhibited compound 48/80-induced degranulation of mast cells and histamine releasing in HMC-1 cells. NK-W decreased mortality and serum histamine releasing in compound 48/80-induced anaphylatic rats in a dose-dependently manner. NK-W also inhibited serum histamine levels in OVA-induced AR mice and improved abnormal histological changes such as expansion of grandular cells and hypertrophy of epithelium in the nasal mucosa. These results indicate that Osterici Radix water extract suppress allergic response through downregulation of mast cell activation. Conclusions : This study suggests that a therapeutic potential of Osterici Radix as a source of anti-allergic agents for use in a number of allergic diseases.

An Investigation of Fluid Mixing with Direct Vessel Injection (직접용기주입에 따른 유체혼합에 관한 연구)

  • Cha, Jong-Hee;Jun, Hyung-Gil
    • Nuclear Engineering and Technology
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    • v.26 no.1
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    • pp.63-77
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    • 1994
  • The objective of this work is to investigate fluid mixing phenomena related to pressurized thermal shock(PTS) in a pressurized water reactor(PWR) vessel downcomer during transient cooldown with direct vessel injection(DVI) using test models. The test model designs were based on ABB Combustion Engineering(C-E) System 80+ reactor geometry. A cold leg small break loss-of-coolant accident(LOCA) md a main steam line teak were selected as the potential PTS events for the C-E System 80+. This work consist of two parts. The first part provides the visualization tests of the fluid mixing between DVI fluid and existing coolant in the downcomer region, and the second part is to compare the results of thermal mixing tests with DVI in the other test model. Row visualization tests with DVI have clarified the physical interaction between DVI fluid and primary coolant during transient cooldown. A significant temperature drop was observed in the downcomer during the tests of a small break LOCA Measured transient temperature profiles agree well with the predictions by the REMIX code for a small break LOCA and with the calculations by the COMMIX-1B code for a steam line break event.

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