• Biomolecules & Therapeutics
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• 제3권3호
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• pp.242-244
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• 1995

In vitro cell culture system has been proposed as a promising alternative model to in vivo skin irritation test. These studies were performed to screen the cytotoxicity effects of surfactants using normal human skin fibroblasts. Cell membrane integrity assessed by the leakage of lactate dehydrogenase (LDH) and mitochondrial integrity by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromides reduction test were affected in a dose dependent manner. The irritation potential of surfactants to human skin patch test, and the changes of capillary permeability by rabbit intradermal safety test were assessed as in vivo methods. Our results suggest that LDH leakage assay and MTT reduction test using cultured human fibroblasts could be predictive for the irritancy of various surfactants in human, and LDH assay is superior correlated with in vivo test (r=0.886) to MTT test with in vivotest (r=0.757).

• Steel and Composite Structures
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• 제6권5호
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• pp.367-386
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• 2006

This paper describes the results of a numerical investigation of the large deflection behaviour of steel beams under fire conditions, taking into consideration the effect of catenary action provided by the surrounding structures. The main focus is on the development, validation and application of a simplified calculation method that may be adopted in design calculations. Because no experimental result is available for validation of the simplified calculation method, the finite element program ABAQUS has been used to simulate the large deflection behaviour of a number of steel beams so as to provide alternative results for validation of the proposed method. Utilising catenary action has the potential of eliminating fire protection to all steel beams without causing structural failure in fire. However, practical application of catenary action will be restricted by concerns over large beam deflection causing integrity failure of the fire resistant compartment and additional cost of strengthening the connections and the surrounding structures to resist the catenary forces in the steel beams. This paper will provide a discussion on practical implications of utilising catenary action in steel beams as a means of eliminating fire protection. A number of examples will then be provided to illustrate the type of steel framed structure that could benefit the most from exploiting catenary action in fire resistant design.

• 정보보호학회논문지
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• 제29권5호
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• pp.949-960
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• 2019

최근 임베디드 시스템은 전자기기의 소형화와 IoT(Internet of Things, 사물인터넷)의 발전과 함께 일상 서비스, 의료, 군사, 자율주행 자동차 등 다양한 분야에서 사용되고 있다. 하지만 임베디드 시스템을 위한 기초적인 보안이 미비하여 보안 사고에 대한 잠재적인 위협이 되고 있다. 이에 본 논문에서는 임베디드 시스템 환경의 무결성을 보호하기 위해 TPM(Trusted Platform Module)을 활용한 보안 부팅을 구현하였다. 제안된 설계 방식은 임베디드 시스템에서 요구되는 가용성을 고려하였으며, Boot 과정에서 TPM을 통해 시스템의 변조를 감지한다. 또한 보안 부팅 간 커널의 AES 암호화를 통해 커널에 대한 기밀성을 강화했다.

• Nuclear Engineering and Technology
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• 제54권8호
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• pp.2960-2973
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• 2022

Since Fukushima nuclear power plant (NPP) accident in 2011, the importance of research on various severe accident phenomena has been emphasized. Particularly, detailed analysis of combustion risk is necessary following the containment damage caused by combustion in the Fukushima accident. Many studies have been conducted to evaluate the risk of local hydrogen concentration increases and flame propagation using computational code. In particular, the potential for combustion by local hydrogen concentration in specific areas within the containment has been emphasized. In this study, the process of flame propagation generated inside a reactor core to containment during a loss of coolant accident (LOCA) was analyzed using MELCOR 2.1 code. Later in the LOCA scenario, it was expected that hydrogen combustion occurred inside the reactor core owing to oxygen inflow through the cold leg break area. The main driving force of the oxygen intrusion is the elevated containment pressure due to the molten corium-concrete interaction. The thermal and mechanical loads caused by the flame threaten the integrity of the containment. Additionally, the containment spray system effectiveness in this situation was evaluated because changes in pressure gradient and concentrations of flammable gases greatly affect the overall behavior of ignition and subsequent containment integrity.

• 말소리와 음성과학
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• 제16권2호
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• pp.1-9
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• 2024

This study investigates the fixed and random factors affecting response patterns of wh-scope interpretations in Gyeongsang Korean. It employed logistic mixed-effects regression models to analyze responses from 24 participants who listened to 40 pre-recorded stimuli from 40 different speakers. The stimuli consisted of an embedded wh-phrase and an interrogative ending marker, "-nkiko," thereby forming a wh-question, specifically a matrix wh-scope. Participants repeated the test three times. The study found that the prominence level of a prosodic phrase composed of an embedded verb and a complementizer was inversely related to responses with wh-questions, as demonstrated through multiple regression analysis in Yun. The test trial significantly impacted the number of responses with wh-questions, increasing from 50.3% in the first trial to 58.8% and 61.2% in subsequent trials. Examination of random subject effects revealed two main factors influencing responses: morpho-syntactic constraints and prosodic structural integrity. These two factors demonstrated the potential to be inversely weighted. Analysis of random stimulus effects suggested that the prominence level had limited effects on response patterns with each stimulus primarily eliciting one type of responses across trials.

• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.1003-1010
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• 2022

The purpose of this study was to examine the phytochemical compounds and antibacterial activity of Coffea robusta leaf extract (RLE). The results indicated that chlorogenic acid (CGA) is a major component of RLE. The minimum inhibitory concentrations (MICs) of RLE against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Salmonella Typhimurium were 6.25, 12.5, 12.5, and 12.5 mg/ml, respectively. RLE effectively damages the bacterial cell membrane integrity, as indicated by the high amounts of proteins and nucleic acids released from the bacteria, and disrupts bacterial cell membrane potential and permeability, as revealed via fluorescence analysis. Cytotoxicity testing showed that RLE is slightly toxic toward HepG2 cells at high concentration but exhibited no toxicity toward Caco2 cells. The results from the present study suggest that RLE has excellent potential applicability as an antimicrobial in the food industry.

• Journal of Microbiology and Biotechnology
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• 제34권8호
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• pp.1680-1687
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• 2024

The diverse pharmacological properties of edible bird's nest (EBN) have been elucidated in recent years; however, investigations into its antibacterial effects are still limited. In the present study, we explored the antibacterial activity of a peptide-rich extract of EBN against Staphylococcus aureus, a notorious pathogen. The EBN extract (EEE) was prepared by soaking EBN in 80% ethanol for 2 days at 60℃. Biochemical analyses showed that peptides at the molecular weight range of 1.7-10 kDa were the major biochemical compounds in the EEE. The extract exhibited strong inhibition against S. aureus at a minimum inhibitory concentration (MIC) of 125 ㎍/ml and a minimum bactericidal concentration (MBC) of 250 ㎍/ml. This activity could be attributed to the impact of the extract on cell membrane integrity and potential, biofilm formation, and reactive oxidative species (ROS) production. Notably, the expression of biofilm- and ROS-associated genes, including intercellular adhesion A (icaA), icaB, icaC, icaD, and superoxide dismutase A (sodA), were deregulated in S. aureus upon the extract treatment. Our findings indicate a noteworthy pharmacological activity of EBN that could have potential application in the control of S. aureus.

• 한국건설관리학회논문집
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• 제23권3호
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• pp.45-55
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• 2022

BIM의 도입에 따라 공간이 개별 객체로 인식되면서 객체화된 공간의 속성정보는 법규검토, 에너지 분석, 피난 경로 분석 등을 위한 기반 데이터로 사용 가능하기에 BIM의 활용성을 넓힐 수 있는 발판을 마련하였다. 그러나 BIM 모델 내 개별 공간 속성의 오기입이나 누락이 없는 시멘틱 무결성(semantic integrity)이 보장되어야 하는데, 다수의 참여자에 의한 수작업으로 진행되는 BIM 모델링 과정 특성 상 설계 오류가 빈번히 발생한다는 문제점이 존재한다. 이를 해결하기 위해 BIM 모델의 공간 정합성 검증을 위한 연구가 다수 진행되었으나, 적용 범위가 한정적이거나 분류 정확도가 낮은 한계점이 존재하였다. 본 연구에서는 공간의 기하정보 뿐 아니라 BIM 모델 내 공간과 부재 간 연결 관계를 Graph Convolutional Networks (GCN) 학습과정에 활용하여 향상된 성능의 공간 자동 분류모델을 구축하고자 하였다. 구축된 GCN 기반 모델의 성능을 공간의 기하정보만으로 학습된 기계학습 모델인 Multi-Layer Perceptron (MLP)과 비교하여 공간 분류 시 연결 관계 적용의 효용성을 검증하고자 하였다. 이를 통해 관계정보 활용 시 약 8% 내외 수준으로 공간 분류 성능이 향상되는 것으로 확인되었다.

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2003년도 가을 학술논문집
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• pp.229-232
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• 2003

고준위 방사성폐기물 처분장에서 적용하고 있는 다중 방벽의 한 부분인 처분 용기는 벤토나이트 완충재의 팽윤과 지압으로부터 폐기물을 역학적으로 안전하게 보호함과 동시에 일정 기간 방사성폐기물의 유출을 억제하는 역할을 한다. 용기는 엄격한 재질 선정과 품질 보증을 거쳐 건전성을 확보하나 보수적인 관점에서 보면 용기 제작 과정이나 수송 중 예상치 못한 사건으로 인해 불량품이 발생할 개연성이 있다. 본 연구에서는 이와 같은 사고 시나리오를 가정할 경우 불량 용기를 포함한 전체 용기에 거치된 방사성폐기물의 시간에 따른 환경 위해도를 평가하였다. 본 연구결과 일부 처분 용기에 초기 파손이 발생하더라도 규제치를 잘 만족하는 것으로 판명되었다.

• Clinics in Shoulder and Elbow
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• 제25권4호
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• pp.257-264
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• 2022

Background: Chronic subscapularis tendon tear (SBT) is a degenerative disease and a common pathologic cause of shoulder pain. Several potential risk factors for chronic SBT have been reported. Although metabolic abnormalities are common risk factors for degenerative disease, their potential etiological roles in chronic SBT remains unclear. The purpose of this study was to investigate potential risk factors for chronic SBT, with particular attention to metabolic factors. Methods: This study evaluated single shoulders of 939 rural residents. Each subject undertook a questionnaire, physical examinations, blood tests, and simple radiographs and magnetic resonance imaging (MRI) evaluations of bilateral shoulders. Subscapularis tendon integrity was determined by MRI findings based on the thickness of the involved tendons. The association strengths of demographic, physical, social, and radiologic factors, comorbidities, severity of rotator cuff tear (RCT), and serologic parameters for SBT were evaluated using logistic regression analyses. The significance of those analyses was set at p<0.05. Results: The prevalence of SBT was 32.2% (302/939). The prevalence of partial- and full-thickness tears was 23.5% (221/939) and 8.6% (81/939), respectively. The prevalence of isolated SBT was 20.2% (190/939), SBT combined with supraspinatus or infraspinatus tendon tear was 11.9% (112/939). In multivariable logistic regression analysis, dominant side involvement (p<0.001), manual labor (p=0.002), diabetes (p<0.001), metabolic syndrome (p<0.001), retraction degree of Patte tendon (p<0.001), posterosuperior RCT (p=0.010), and biceps tendon injury (p<0.001) were significantly associated with SBT. Conclusions: Metabolic syndrome is a potential risk factor for SBT, as are these factors: overuse activity, diabetes, posterosuperior RCT, increased retraction of posterosuperior rotator cuff tendon, and biceps tendon injury.