• 한국환경농학회지
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• 제22권3호
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• pp.197-202
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• 2003

Phosphorus is one of the major plant growth limiting nutrients, despite being abundant in soils in both inorganic and organic forms. Phosphobioinoculants in the form of microorganisms can help in increasing the availability of accumulated phosphates for plant growth by solubilization. Penicillium oxalicum CBPSTsa, isolated from paddy rhizosphere, was studied for its phosphate solubilization. The influence of various carbon sources like glucose, sucrose, mannitol and sorbitol and nitrogen sources like arginine, sodium nitrate, potassium nitrate, ammonium chloride and ammonium sulphate were evaluated using liquid media with tricalcium phosphate (Ca-P), ferric phosphate (Fe-P) and aluminium phosphate (Al-P). Maximum soluble phosphate of 824 mg/L was found in the amendment of sucrose-sodium nitrate from 5 g/L of Ca-P. Mannitol, sorbitol, and ariginine were poor in phosphate solubilization. While sucrose was better carbon source in solubilization of Ca-P and Al-P, glucose fared better in solubilization of Fe-P. Though all the nitrogen sources enhanced P solubilization, nitrates were better than ammonium In the amendments of ammonium chloride and ammonium sulphate, higher uptake of available phosphates by the fungus was found, and this resulted in depletion of available P in Fe-P amendment Phosphate solubilization was accompanied by acidification of the media, and the highest pH decrease was observed in glucose amendment Among the nitrogen sources, ammonium chloride favored greater pH decrease.

• The Korean Journal of Physiology
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• 제9권1호
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• pp.1-11
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• 1975

The present experiments were carried out to investigate the active center of sodium and potassium ion activated adenosine triphosphatase. An ATPase, activated by sodium ion Plus potassium ion in the presence of magnesium ion, and inhibited by ouabain, has been obtained from rabbit red cell ghosts. The ATPase activity was measured by inorganie phosphate released from ATP. From this values of the measured inorganic phosphate, the activity of ATPase was calculated. The following results were observed. 1. The activity of $(Na^++K^+)-ATPase$ is inhibited by ouabain. This effect may not be due to an effect on sulfhydryl groups, amino groups, carboxyl groups, imidazole groups and hydroxyl groups. 2. The $(Na^++K^+)$-activated enzyme system is inhibited by p-chloromercuribenzoate and by d nitroflurobenzene, and this effect may be due to an effect on sulfhydryl groups. These results indicate that the sulfhydryl groups is attached to sodium-potassium dependent adenosine triphosphate, an aspect of the pump. 3. The $(Na^++K^+)-activated$ enzyme system is inhibited by maleic anhydride and this inhibition is reversed by lysine. This Seems to indicate that the active center of this enzyme is the amino groups. 4. The $(Na^++K^+)$-activated enzyme system is inhibited by iodoacetamide and this inhibition is reversed by the simultaneous present of cysteine and aspartic acid in the suspension medium. This result indicates that this enzyme contains sulfhydryl groups and carboxyl groups. 5. The $(Na^++K^+)-ATPase$ activity is accelerated by adrenaline and this effect is abolished by aspartic acid. This effect of aspartic acid indicate that carboxyl group might be involved in the hydrolysis of ATP by the enzyme system. On the hydrolysis of ATP by the enzyme system. On the basis of these experiments it f·as suggested that the active center of $(Na^++K^+)-activated$ ATPase contains sulfhydryl groups, amino groups and carboxyl groups.

• 한국가축번식학회지
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• 제20권3호
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• pp.251-258
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• 1996

본 연구는 착성전 Rat 8-세포기 난자의 체외배양시 glucose95.56 mM와 0mM)와 phosphate(1.19mM와 0 mM)의 억제효과를 규명하기 위하여 실시하였다. 48시간 배양후 발육율은 glucose＋phosphate군에서는 37%(31/84), glucose군은 70%(64/91), phosphate군은 69% (59/85) 그리고 glucose와 phosphate가 없는 무처리군에서는 77%(67/85)를 나타냈다. Glucose＋phosphate군의 발육율은 다른 처리군에 비해 유의적으로 낮았고, 그 외 처리군 사이에는 유의성이 없었다. 발육난자의 핵염색에 의한 세포수의 측정에서는 glucose군에서 가장 많은 세포수를 나타냈고 (29.3$\pm$0.97, P<.001), 그외 처리에서는 유의성이 없었다 (glucose＋phosphate, 17.5$\pm$1.04; phosphate, 18.6$\pm$1.01; no glucosephosphate, 19.8$\pm$1.01). 본 실험의 결과로 glucose와 phosphate는 Rat 8-세포기 난자의 발육에 억제효과를 나타냈고, glucose는 난자의 세포분열을 증진시키는 효과가 있는 것으로 나타났다.

• 한국토양비료학회지
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• 제34권3호
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• pp.173-177
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• 2001

This study was conducted to investigate a potential utilitization of multivariate statistical analysis(Factor analysis, Discrimination analysis) on interpretation of rice plant lodging reason. Rice plants were sampled in paddy around Taegu city at from 25 to 29 of September in 2000. Mineral nutrient content(phosphate, potassium) of rice plant were significantly higher at 99% level, Silicate content were lower at 95% level in lodged samples than in normal. Plant characteristics associate with lodging(Culm length, second and third internode length, bight of center gravity) were significantly longer in lodged rice plant than in non lodged. Result of Factor analysis were that first principle component were culm length, second(N2) and third internode length(N3), second principle component were Ca content, first internode length(N1) and N3/culm length, third principle component were center gravity length(G) and G/culm length, fourth were nitrogen, phosphate, and potassium content, fifth were N2/culm length, N2+N3/culm length, Sixth was silicate content of rice plant. Linear discriminant equation distinguished lodged rice plants with non lodged rice plants very well. Prediction value was 100%, most explainable variable were phosphate content, culm length and third length.

• The Korean Journal of Ecology
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• 제10권3호
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• pp.129-138
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• 1987

In a red pine (Pinus densiflora) forest, changes of pH, electric conductivity, total carbon, total nitrogen, available phosphate and available potassium in soil and runoff have been studied at intervals for 1 year after early spring fire. Phytimasses of herb and shrub were measured following the current and the subsequent year. The pH, E.C., total nitrogen and phosphate of soil in burned site wee 1.1, 1.5, 1.6 and 2.0 times higher than in unburned site, respectively. But potassium showed no significant difference. A rise in pH, E.C., and total nitrogen in burned site were maintained throught the study period while phosphate maintained 4 months after the fire. The E.C., total carbon, $NO_2-N$ and $NH_4-N$ of runoff in burned site were 1.3, 1.3, 1.3 and 29.0 times higher than in unburned site, respectively, while $NO_3-N$ in unburned site was 4 times higher than in burned site. In burned site, phytomasses of herb and shrub were 148 and 33% of unburned site in a current year and 107 and 51% in a subsequent year, respectively. The considerable amount of increase in soil nutrient after the fire was conserved by the uptake of the fast regrowing plants and by the immobilization of $NH_4=N$.

• 한국토양비료학회지
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• 제49권2호
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• pp.194-199
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• 2016

In an experiment conducted at the research field of the National Institute of Agricultural Science, we investigated the effects of mineral fertilizer and rice straw compost on exchangeable potassium and K balances, and rice grain yield under a rice single system. The treatments were no fertilization (No fert.), inorganic fertilization (N), inorganic fertilizer (N, P, K) plus rice straw compost at rates of 7.5, 15.0, 22.5, and $30.0ton\;ha^{-1}$ (NPKC7.5, NPKC15.0, NPKC22.5, and NPKC30.0, respectively). The inorganic fertilizers(N, P, K) were added with standard fertilizer application rate in which nitrogen (N), phosphate ($P_2O_5$), and potassium ($K_2O$) were applied with $75{\sim}150kg\;ha^{-1}$, $70{\sim}86kg\;ha^{-1}$, $75{\sim}86kg\;ha^{-1}$, respectively. Exchangeable potassium for NPKC15.0 NPKC22.5, and NPK30.0 treatments was higher by $0.05{\sim}0.19cmol_c\;kg^{-1}$ than that of NPKC7.5 treatment. Increasing levels of rice straw compost resulted in an increase in the K balance from - $19.9kg\;ha^{-1}yr^{-1}$ (No fert.) to $41.9kg\;ha^{-1}yr^{-1}$ at NPKC22.5 treatment and $62.9kg\;ha^{-1}$ at NPKC30.0 treatment. Continuous application of rice straw compost with NPK fertilizers affected significantly the rice grain yields. The result of the study imply that the application of more than $22.5ton\;ha^{-1}$ of rice straw compost with NPK fertilizers are recommended as the best fertilization practice for enhancement of crop production and K supplying power of soil in the continuous rice cropping system.

• KSBB Journal
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• 제14권1호
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• pp.36-44
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• 1999

고지혈증 치료제인 Lovastatin을 Aspergillus terreus로부터 생산하기 위해 최적의 탄소원, 질소원, 인산염 및 아미노산을 선정하였다. 단일 탄소원에서는 글리세롤 45mL/L가 Lovastatin 256mg/L로 포도당 45g/L의 기본배지보다 약 3.5배 이상의 높은 Lovastatin 생산량을 나타내었다. 탄소원이 지방산 합성에 이용되는 것을 차단하고 Lovastatin으로의 합성을 촉진하기 위해 기름 증류를 탄소원으로 이용한 결과, 높은 Lovastatin 생산량과 균재량을 나타내었으나, 글리세롤 45mL/L가 Lovastatin 생산량이 낮았다 포도당 50g/L와 글리세롤 50mL/L를 혼합한 복합 탄소원이 Lovastatin 298mg/L로 기본배지에 비해 약 4배 이상의 가장 높은 Lovastatin 생산량을 나타냈었다. 질소원의 영향은 뚜렷하여 질소원에 따라 Lovastatin 생산량이 큰 차이를 나타내었으며, 그 중에서 peptonized milk가 가장 Lovastatin 생산량이 높았다. 인산염 중에서는 $K_2HPO_4$가 가장 높은 Lovastatin 생산량을 나타내었다. 이상에서 얻어진 최적 배지에 각종 아미노산을 첨가한 결과 L-hisbhine과 L-tryptophan이 가장 효과적이었으며, 최적 농도는 둘다 6g/L였다.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.211-217
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• 2013

Corynebacterium glutamicum is one of the well-studied industrial strain that is used for the production of nucleotides and amino acids. Recently, it has also been studied as a possible producer of organic acids such as succinic acid, based on its ability to produce organic acids under an oxygen deprivation condition. In this study, we conducted the optimization of medium components for improved succinate production from C. glutamicum under an oxygen deprivation condition by Plackett-Burman design and applied a response surface methodology. A Plackett-Burman design for ten factors such as glucose, ammonium sulfate, magnesium sulfate, potassium phosphate ($K_2HPO_4$ and $KH_2PO_4$), iron sulfate, manganese sulfate, biotin, thiamine, and sodium bicarbonate was applied to evaluate the effects on succinate production. Glucose, ammonium sulfate, magnesium sulfate, and dipotassium phosphate were found to have significant influence on succinate production, and the optimal concentrations of these four factors were sequentially investigated by the response surface methodology using a Box-Behnken design. The optimal medium components obtained for achieving maximum concentration of succinic acid were as follows: glucose 10 g/l, magnesium sulfate 0.5 g/l, dipotassium phosphate ($K_2HPO_4$) 0.75 g/l, potassium dihydrogen phosphate ($KH_2PO_4$) 0.5 g/l, iron sulfate 6 mg/l, manganese sulfate 4.2 mg/l, biotin 0.2 mg/l, thiamine 0.2 mg/l, and sodium bicarbonate 100 mM. The parameters that differed from a normal BT medium were glucose changed from 40 g/l to 10 g/l, dipotassium phosphate ($K_2HPO_4$) 0.5 g/l changed to 0.75 g/l, and ammonium sulfate ($(NH_4)_2SO_4$) 7 g/l changed to 0 g/l. Under these conditions, the final succinic acid concentration was 16.3 mM, which is about 1.46 fold higher than the original medium (11.1 mM) at 24 h. This work showed the improvement of succinate production by a simple change of media components deduced from sequential optimization.

• 한국토양비료학회지
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• 제33권5호
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• pp.333-339
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• 2000

경북지역의 마늘 주산지인 의성과 예천에서 마늘재배지를 논, 밭토양으로 구분하여 토양의 화학성과 마늘의 생육 및 품질과의 상관관계를 조사하였다. 토양의 화학성은 밭토양에 비해 논토양이 유효인산, $SO_4{^2-}$ 및 K의 함량이 높았으며 과다집적된 경향이었다. 의성 마늘재배지 토양이 예천 마늘재배지 토양에 비해 pH가 높고 유효인산의 함량이 낮은 것으로 나타났다. 마늘성분 중 pyruvate 함량이 예천지역의 $269{\mu}{\cdot}mole\;kg^{-1}$에 비해 의성지역이 $351{\mu}{\cdot}mole\;kg^{-1}$으로 높게 나타났다. 토양 중 유효인산, K, Mg와 마늘의 생육특성과는 고도의 유의성이 인정되었다. 마늘 재배지에서 인산의 축적은 마늘의 품질 및 생육을 저해하는 것으로 나타났다.

• 화훼연구
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• 제19권3호
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• pp.144-150
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• 2011

본 연구는 염류집적 국화 재배지 토양적응성 인산분해미생물 탐색하고 선발된 미생물 시용을 통한 염류집적 국화 재배지 토양에서의 토양 화학성의 변화를 조사하였으며 이를 통하여 염류집적 국화 재배지 토양 환경 개선 기술을 개발하고자 수행되었다. 시험에 사용된 인산분해미생물은 염류집적토양에서 분리된 Pseudomonas putida(KSJ11), Acinetobacter calcoaceticus (KSJ3) 및 Acinetobacter calcoaceticus (WP20) 3종류이었으며 미생물의 제형은 버미큘라이트에 혼합되어 있는 상용화된 제품을 이용하였다. 시험장소는 광주광역시 광산구 소재 신우화훼농장의 15년간 작물이 재배되어 염류집적현상이 나타나는 국화재배지에서 처리구 $82m^2$에 각각의 미생물 제재 250 L씩 시용하였다. 염류집적이 이루어진 국화재배온실에 처리된 인산분해미생물 Acinetobacter calcoaceticus(KSJ3; WP20)는 유효인산을 효율적으로 분해하는 것으로 나타났으며, 특히 Acinetobacter calcoaceticus(WP20)는 염류의 분해능력이 높았다. 인산분해미생물 시용에 따라 토양내 칼륨, 칼슘 및 마그네슘의 함량의 증가가 A. calcoaceticus(KSJ3; WP20)처리구에서 뚜렷하게 나타났으며 이러한 변화의 영향으로 판단되는 토양내 전기전도도도 증가되었다. 또한 인산분해미생물 시용은 선충밀도의 감소효과를 나타내어 토양환경개선을 위한 재료로 활용될 가능성을 나타내었다. 결과적으로 염류집적이 이루어진 국화재배 온실에서의 인산분해미생물 시용은 처리된 미생물의 종류에 따라 차이를 나타내었지만 토양의 유효인산량 증가와 양이온의 유용화에는 분명한 효과를 나타내었다. 따라서 염류집적 토양에서의 인산분해미생물 시용은 토양양분의 효율적인 사용을 가능하게 할 수 있는 방안이 될 수 있기 때문에 시비량 절감 등의 방법으로 활용할 수 있다고 판단되었다.