• Journal of Food Hygiene and Safety
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• v.30 no.3
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• pp.249-257
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• 2015

The purposes of this study were to develop a small scale post-harvest facility, and consequently to evaluate the effects of applying the facility along with hygiene education on the level of microbial safety in Korean leeks production. A total of 135 samples were collected at three Korean leeks farms in Yangju, Gyeonggi province. Food safety indicators (Aerobic plate count (APC), coliform count, and Escherichia coli) and foodborne pathogens (E. coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus) on/in the samples were assessed. The microbial load measured as APC with harvesting tools such as comb, chopping board, and knife, at the farms where the small scale post-harvest facility had been operated (Farms A and B) was lower than that at another farm having no post-harvest facility (Farm C) by 1.44~2.33 log CFU / $100cm^2$. Moreover, the chopping board from Farm C was observed being contaminated with B. cereus at 6.03 log CFU / $100cm^2$. The coliform counts from the samples increased by 0.57~1.89 log CFU/g after leeks was submerged in ground water for washing. E. coli was recovered from leeks, soil, and the ground water used in the washing process, while no E. coli O157:H7, Salmonella spp., and L. monocytogenes was detected. Our results indicated that the small scale post-harvest facility developed in this study as well as the hygiene education played an important role in enhancing the level of microbial food safety in the leeks production environment. However, a disinfection technique could be needed during the washing step in order to prevent a potential contamination.

• Journal of Embryo Transfer
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• v.11 no.3
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• pp.249-258
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• 1996

To improve the efficiency of in vitro production of embryos with follicular oocytes in Korean Native cows, the recovery rates, in vitro maturation, fertilization and development, and the time required for collecting and processing oocytes by aspiration with or without slicing were evaluated comparatively. The ovaries were obtained from a local abattoir and placed in physiological saline at 25~28$^{\circ}C$ and brought to the laboratory within 3 hrs. The oocytes were collected by aspiration of follicles(2~6mm) with or without slicing ovaries after aspiration, and classified into Grade I, Grade II, Denuded, Expanded oocytes by the morphology of cumulus cells attached and the homogeneity of cytoplasmic granules. Also the time required for each step of collecting and processing oocytes were measured. The cumulus cells were removed in some Grade I oocytes to measure their size and nuclear configuration before and after in vitro maturation. The Grade I oocytes were matured in vitro(IVM) for 24 hrs. in TGM-199 supplemented with 35$\mu$g /ml FSH, 10$\mu$g /ml LH, 1 $\mu$g /ml at 39$^{\circ}C$ under 5% C02 in air. They were fertilized in vitro(IVF) by epididymal spermatozoa treated with heparin for 24hrs. and then the zygotes were cocultured in vitro (IVC) with bovine oviductal epithelial cells for 10 days. The results obtained were as follows: The number of oocytes recovered per ovary was averaged 6.6 by aspiration and 11.2 by slicing post aspiration, which summed to 17.8. The number of Grade I oocytes recovered per ovary was averaged 3.1 by aspiration and 3.6 by slicing, which summed to 6.7. The percentage of Grade I to total oocytes recovered was significantly(P<0.05) higher as 48.0 % in aspiration than 31.6% in slicing post aspiration. The time requlred for recovering a Grade I oocyte by aspiration and slicing was 1.1 and 2.5 min, respectively. The mean diameter of Grade I oocytes by aspiration and slicing was similar as 148.7 and 151.5$\mu$m, respectively. The percentage of Metaphase II stage oocytes after IVM for 24 hours was significantly (P

• Broadcasting and Media Magazine
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• v.22 no.3
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• pp.69-75
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• 2017

SDR 영상과 함께 HDR 버전 제작을 함께 요청하는 프로젝트가 점점 늘어나는 추세다. 어느 정도 규모의 업체에서는 고가의 HDR 레퍼런스 모니터와 라이선스 비용을 지불하고 비교적 편리하게 작업할 수 있다. 상황이 그렇지 못할 경우 최소의 비용으로 장비를 구매하고, 기존 장비와 호환성을 점검하고, 컬러링 어플리케이션에서 지원하는 기능을 활용하여 최선의 워크플로를 구성해야 한다. 또한 SDR 버전을 HDR 버전으로 제작하거나 HDR 영상을 SDR 버전으로 변환할 때 장면마다 다시 보정하는 일을 최소화하기 위한 세심한 보정이 필요하다. 본 원고에서는 동시에 촬영된 BT.709 기반의 HD 프록시 영상과 RAW로 촬영된 UHD 원본 영상을 활용하여 변환 작업에서 주의 깊게 살펴보아야 할 사항들을 알아보도록 하겠다.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2005.06a
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• pp.333-333
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2003.05a
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• pp.63-64
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• 2003

• Phonetics and Speech Sciences
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• v.2 no.3
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• pp.29-36
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• 2010

The purposes of this study are (1) to see if language transfer effect is found in Korean speakers' pronunciation of English stops and to correct them and (2) to investigate the effectiveness of mimicry training and Speech Analyzer training on subjects' pronunciation of English stops. For these purposes, 20 Korean speakers' VOT values of English stops were measured using Speech Analyzer and their post-training production was compared with their pre-training production. The result shows that Korean speakers have no difficulty in correcting pronunciation errors of English voiceless stops and voiced stops and such a result indicates that language transfer effect is not noticed as expected. In addition, the result of pronunciation training shows that the training using Speech Analyzer is more effective than mimicry training.

• Phonetics and Speech Sciences
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• v.1 no.4
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• pp.11-15
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• 2009

The aim of this study was to identify production difficulties of Korean learners of English in their articulation of English bilabial stops /p, b/ and labiodental fricatives /f, v/. Sixty non-sense syllables and twelve words were produced three times by nine graduate students. Test scores were measured from the score board made by FluSpeak, a speech training software program, which was designed for English pronunciation practice and improvement. Results show that 1) the subjects had lower scores in producing /p, b/ than /f, v/ from all positions, and 2) subjects had lower scores in medial (inter-vocalic) position than in initial (pre-vocalic) position and in final (post-vocalic) position when they produced /p/, /b/, /f/, and /v/. The results suggest that on the whole, Korean learners of English have much difficulty in producing /b/ and that they also have more articulatory problems in intervocalic than in the other positions when they produce these bilabial stops and labiodental fricatives.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.1
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• pp.34-39
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• 2006

Molecular biology and microalgal biotechnology have the potential to play a major role in improving the production efficiency of a vast variety of products including functional foods, industrial chemicals, compounds with therapeutic applications and bioremediation solutions from a virtually untapped source. Microalgae are a source of natural products and have been recently studied for biotechnological applications. Efficient genetic transformation systems in microalgae are necessary to enhance their potential to be used for human health. A microalga such as Chlarella is a eukaryotic organism sharing its metabolic pathways with higher plants. This microalga is capable of expressing, glycosylating, and correctly processing proteins which normally undergo post-translational modification. Moreover, it can be cultured inexpensively because it requires only limited amount of sunlight and carbon dioxide as energy sources. Because of these advantages, Chlarella may be of great potential interest in biotechnology as a good candidate for bioreactor in the production of pharmaceutical and industrial compounds for human functional foods. Here, we briefly discuss recent progress in microalgal transgenesis that has utilized molecular biology to produce functional proteins and bioactive compounds.

• Molecules and Cells
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• v.38 no.7
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• pp.597-603
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• 2015

Biosynthesis of the phytohormone ethylene is under tight regulation to satisfy the need for appropriate levels of ethylene in plants in response to exogenous and endogenous stimuli. The enzyme 1-aminocyclopropane-1-carboxylic acid synthase (ACS), which catalyzes the rate-limiting step of ethylene biosynthesis, plays a central role to regulate ethylene production through changes in ACS gene expression levels and the activity of the enzyme. Together with molecular genetic studies suggesting the roles of post-translational modification of the ACS, newly emerging evidence strongly suggests that the regulation of ACS protein stability is an alternative mechanism that controls ethylene production, in addition to the transcriptional regulation of ACS genes. In this review, recent new insight into the regulation of ACS protein turnover is highlighted, with a special focus on the roles of phosphorylation, ubiquitination, and novel components that regulate the turnover of ACS proteins. The prospect of cross-talk between ethylene biosynthesis and other signaling pathways to control turnover of the ACS protein is also considered.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.915-922
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• 2009

Ruminants possess the capacity to digest very large amounts of starch. However, in many cases diets approach 60% starch and even small inefficiencies present opportunities for energetic losses. Ruminal starch digestion is typically 75-80% of starch intake. On average, 35-60% of starch entering the small intestine is degraded. Of the fraction that escapes small-intestinal digestion, 35-50% is degraded in the large intestine. The low digestibility in the large intestine and the inability to reclaim microbial cells imposes a large toll on post-ruminal digestive efficiency. Therefore, digestibility in the small intestine must be optimized. The process of starch assimilation in the ruminant is complex and remains an avenue by which increases in production efficiency can be gained. A more thorough description of these processes is needed before we can accurately predict digestion occurring in the small intestine and formulate diets to optimize site of starch digestion.