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http://dx.doi.org/10.13103/JFHS.2015.30.3.249

Effects of Small Scale Post-Harvest Facility and Hygiene Education on the Level of Microbial Safety in Korean Leeks Production  

Kim, Se-Ri (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Kim, Jin-Bae (Technology Services Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Lee, Hyo-Sup (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Lee, Eun-Sun (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Kim, Won-Il (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Ryu, Song-Hee (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Ha, Jihyung (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Kim, Hwang-Yong (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Ryu, Jae-Gee (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAAS, RDA)
Publication Information
Journal of Food Hygiene and Safety / v.30, no.3, 2015 , pp. 249-257 More about this Journal
Abstract
The purposes of this study were to develop a small scale post-harvest facility, and consequently to evaluate the effects of applying the facility along with hygiene education on the level of microbial safety in Korean leeks production. A total of 135 samples were collected at three Korean leeks farms in Yangju, Gyeonggi province. Food safety indicators (Aerobic plate count (APC), coliform count, and Escherichia coli) and foodborne pathogens (E. coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus) on/in the samples were assessed. The microbial load measured as APC with harvesting tools such as comb, chopping board, and knife, at the farms where the small scale post-harvest facility had been operated (Farms A and B) was lower than that at another farm having no post-harvest facility (Farm C) by 1.44~2.33 log CFU / $100cm^2$. Moreover, the chopping board from Farm C was observed being contaminated with B. cereus at 6.03 log CFU / $100cm^2$. The coliform counts from the samples increased by 0.57~1.89 log CFU/g after leeks was submerged in ground water for washing. E. coli was recovered from leeks, soil, and the ground water used in the washing process, while no E. coli O157:H7, Salmonella spp., and L. monocytogenes was detected. Our results indicated that the small scale post-harvest facility developed in this study as well as the hygiene education played an important role in enhancing the level of microbial food safety in the leeks production environment. However, a disinfection technique could be needed during the washing step in order to prevent a potential contamination.
Keywords
Korean leeks; microorganisms; small scale post harvest facility;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Kim S.R., Lee H.S., Kim W.I., Ryu S.H., Kim H.Y., Ryu J.G.: Perspectives on the research to enhance microbial safety of agricultural produce in post harvest Management. Safe Food, 9, 13-19 (2014).
2 FDA: Guidance for Industry: Guide to Minimize Microbial Food Safety Hazards of Leafy Greens; Draft Guidance Available from: http://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocuments/ProduceandPlanProducts/ucm174200.htm Accessed January 15, 2010 (2009).
3 CDC: Update on Multi-State Outbreak of E.coliO157:H7 Infections From Fresh Spinach, October 6, 2006. Available from: http://www.cdc.gov/ecoli/2006/september/updates/100606.htm Accessed Febrary 20, 2010 (2006).
4 Patel J. and Sharma M.: Differences in attachment of Salmonella enteric serovars to cabbage and lettuce leaves. Int. J. Food Microbiol., 139, 41-47 (2010).   DOI   ScienceOn
5 Hong C.K., Seo Y.H., Choi C.M., Hwang I.S., and Kim M.S.: Microbial quality of fresh vegetables and fruits in Seoul, Korea, Korean J. Fd Hyg. Safety, 27, 24-29 (2012).   DOI
6 KFDA: Korean Food code. Korea Food and Drug Administration, Seoul, Korea. pp.75-105 (2002).
7 Choo E.Y., Jang S.S., Kim K.S., Lee K.G., Heu S.G. and Ryu, S.R.: Prevlence and genetic diversity of Bacillus cereus in dried red pepper in Korea. J. Food Prot., 70, 917-922 (2007).   DOI
8 Kim S.R., Lee J.Y., Lee S.H., Ko H.S., Yoon Y.H., Kwon S.H., Ryu K.Y., Yun H.J., Kim W.I., Yun J.C., Kim D.H. and Chung D.H.: Distribution of Hazardous Microorganisms in Perilla Leaf Cultivation Areas. Korean J. Food Sci. Technol., 43, 243-248 (2001).
9 Park S.H., Kim J.S., Kim K.Y., Chung D.H. and Sim W.B.: Identification of toxin gene and antibiotic resistance of Staphylococcus aureus isolated from agricultural product cultivation environments. J. Environ Health Sci., 39, 465-473 (2013).
10 Jensen D.A., Friedrich L.M., Harris L.J., Danyluk M.D., and Schaffner D.W.: Cross contamination of Escherichia coli O157:H7 between lettuce and wash water during home-scale washing. Food Microbiol., 6, 428-433 (2015).
11 Johnston L.M., Jaykus L.A., Moll D., Martinez M.C., Anciso J., Mora B. and Moe C.L.:. A field study of the microbiological quality of fresh produce. J. Food Prot., 68, 1840-1847 (2005).   DOI
12 Ailes E.C., Leon J.S. Jaykus L.A., Johnston L.M., Clayton H.A., Blanding S., Kleinbaum D.G., Backer L.C., and Moe C.L.: Microbial concentrations on fresh produce are affected by postharvest processing, importation, and season. J. Food Prot., 71, 2389-2397 (2008).   DOI
13 Solomon E.B., Yaron S. and Mattews K.R.: Transmission of Escherichia coli O157:H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization. Appl. Environ. Microbiol., 68, 397-400 (2002).   DOI
14 Burnett S.L. and Beuchat L.R.: Human pathogens associated with raw produce and unpasteurized juices and difficulties in decontamination. J. Int. Microbiol. Biotechnol., 27, 104-110 (2001).   DOI
15 Bae Y.M. and Lee S.Y.: Microbial quality of fresh produce in Korea and decontamination technologies, Safe Food, 4, 33-39 (2009).
16 Jung S.H., Hur M.J, Ju J.H., Kim K.A., Oh S.S., Go J.M., Kim Y.H., and Im J.: Microbiological evaluation of raw vegetables. Korean J. Fd Hyg. Safety, 21, 250-257 (2006).
17 FDA: Guidance for industry, Guide to minimize microbial food safety hazard for fresh fruits and vegetables. Available From: http://csan.fda.gov. Accessed Oct. 26, (2005).
18 Kim S.Y.: Indication system for the verification in agricultural food safety. In: Symposium on GAP application strategy for the safety agricultural production. July 29, Gyeongsang National University, Jinju, Korea. The Center of Agri-food Safty. Jinju, Korea pp. 23-42 (2004).
19 Rural development Administration: Good Agricultural Practices Standard: Notification No. 2010-32 of the Rural development Administration. Available From: http://www.rda.go.kr/ Accessed Oct. 26 2014, (2010).
20 National Agricultural Products Quality Management Service: Good Agricultural Practices certification system. Available From: http://www.gap.go.kr. Accessed April. 26 2015, (2015).
21 Rural development Administration: Good Agricultural Practices Standard: Notification No. 2014-33 of the Rural development Administration. Available From: http://www.rda.go.kr. Accessed Oct. 26 2014, (2014).
22 Anonymous: Guidelines for effectiveness testing of surgical hand scrub (glove juice test). Fed. Regist. 43, 1242-1243 (1978).
23 Liu C., Hofstra N., and Franz E.: Impacts of climate change on the microbial safety of pre-harvest leafy green vegetables as indicated by Escherichia coli O157 and Salmonella spp. Int. J. Food Microbiol., 163, 119-128 (2013).   DOI
24 Oliveira M., Usall J., Vinas I., Solsona C. and Abadias M.: Transfer of Listeria innocua from contaminated compost and irrigation water to lettuce leaves. Food Microbiol., 28, 590-596 (2011).   DOI
25 Solomon E.B., Potenski C.J. and Mattews K.R.: Effect of irrigation method on transmission to and persistence of Escherichia coli O157:H7 on lettuce. J. Food. Prot., 65, 673-676 (2002).   DOI