• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.457-462
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• 2003

The complete nucleotide sequence of a plasmid, pMG1, isolated from Bifidobacterium longum MG1 has been determined. This plasmid, composed of 3,862 base pairs with 65.1% of G+C content. harbors two major open reading frames (ORF) encoding putative proteins of 29 kDa (ORF I) and 71 kDa (ORF II). ORF I showed relatively high amino acid sequence homology with replication proteins of other plasmids from Gr Im-positive and -negative bacteria. Upstream of ORF I, four sets of tandem repeat sequences resembling the iteron structure of related plasmids were found. S1 endonuclease treatment and Southern blot analysis revealed that pMG1 accumulates single-stranded DNA (ssDNA) intermediate, which indicate i the rolling circle replication (RCR) mechanism of this plasmid. Homology search indicated that ORF II encodes plasmid mobilization protein, and the presence of highly conserved oriT sequence in the upstream of this gene supported this assumption. RT-PCR showed that only ORF I is expressed in vivo. Based on these results, pMG 1 was exploited to construct a shuttle vector, pBES2. It was successfully transformed into Bifidobacterium and maintained stably.

• Journal of information and communication convergence engineering
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• v.10 no.2
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• pp.210-214
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• 2012

A microtiter well plate DNA hybridization method using Mycobacterium kansasii-specific rpoB DNA probe (kanp) were evaluated for the detection of M. kansasii from culture isolates. Among the 201 isolates tested by this method, 27 strains show positive results for M. kansasii, but the other 174 isolates were negative results for M. kansasii. This result was consistent with partial rpoB sequence analysis of M. kansasii and the result of biochemical tests. The negative strains by this DNA-DNA hybridization method were identified as Mycobacterium tuberculosis (159 strains), Mycobacterium avim (5 strains), Mycobacterium intracellulare (8 strains), and Mycobacterium flavescens (2 strain) by rpoB DNA sequence analysis. Due to high sensitivity and specificity of this test result, we suggest that DNA-DNA hybridization method using rpoB DNA probes of M. kansasii could be used for the rapid and convenient detection of M. kansasii.

• Reproductive and Developmental Biology
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• v.38 no.2
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• pp.71-77
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• 2014

The specific genetic modification in porcine somatic cells by gene targeting has been very difficult because of low efficiency of homologous recombination. To improve gene targeting, we designed three kinds of knock-out vectors with ${\alpha}1,3$-galactosyltransferase gene (${\alpha}1,3$-GT gene), DT-A/pGT5'/neo/pGT3', DT-A/NLS/pGT5'/neo/pGT3' and pGT5'/neo/ pGT3'/NLS. The knock-out vectors consisted of a 4.8-kb fragment as the 5' recombination arm (pGT5') and a 1.9-kb fragment as the 3' recombination arm (pGT3'). We used the neomycin resistance gene (neo) as a positive selectable marker and the diphtheria toxin A (DT-A) gene as a negative selectable marker. These vectors have a neo gene insertion in exon 9 for inactivation of ${\alpha}1,3$-GT locus. DT-A/pGT5'/neo/pGT3' vector contain only positive-negative selection marker with conventional targeting vector. DT-A/NLS/pGT5'/neo/pGT3' vector contain positive-negative selection marker and NLS sequences in upstream of 5' recombination arm which enhances nuclear transport of foreign DNA into bovine somatic cells. pGT5'/neo/pGT3'/NLS vector contain only positive selection marker and NLS sequence in downstream of 3' recombination arm, not contain negative selectable marker. For transfection, linearzed vectors were introduced into porcine ear fibroblasts by electroporation. After 48 hours, the transfected cells were selected with $300{\mu}g/ml$ G418 during 12 day. The G418-resistant colonies were picked, of which 5 colonies were positive for ${\alpha}1,3$-GT gene disruption in 3' PCR and southern blot screening. Three knock-out somatic cells were obtained from DT-A/NLS/ pGT5'/neo/pGT3' knock-out vector. Thus, these data indicate that gene targeting vector using nuclear localization signal and negative selection marker improve targeting efficiency in porcine somatic cells.

• Korean Institute of Interior Design Journal
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• v.21 no.5
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• pp.181-188
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• 2012

Body movements of humans have emotions and these movements have meanings of 'Living Spirits' and 'Emotions'. Also emotions of humans indicate all body movements which are made within the environment. This study aims at providing new potentials to emotional design research methods not only by understanding the characteristics of visual perception according to body movements and but by investigating correlations between sequence which arise by the visual perception and emotions through experiments. As for the scope and method of this study, the emotional space designs were analyzed through the emotional theory study, SD method was used to evaluate emotions and the movements in the modern village and traditional village with similar elements were compared and analyzed for the empirical study. As a result, first it was confirmed waling hours in the traditional village took more than the modern village and it is thought movements of humans are affected by the visual environments. Second, it was confirmed values of the emotional evaluation were higher in the traditional village than the modern village. Third, according to a result of the correlation analysis between space sequence and emotions, it was closed with negative emotions such as 'Closed', and 'Complicated' from positive emotional words such as 'Natural'. 'Open', 'Curious', etc. as spaces are experienced more through movements in the modern village. On the contrary, the emotional intensity of the positive words such as 'In Harmony', 'Beautiful' and 'Warn' through movements in the traditional village increased as spaces were experienced more. As a result of this study, it was confirmed the time, space and emotions have correlations each other.

• Proceedings of the KIPE Conference
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• 2013.07a
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• pp.244-245
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• 2013

This paper proposes a control algorithm for permanent magnet synchronous generator with a back-to-back three-level neutral-point clamped voltage source converter in a medium-voltage offshore wind power system under unbalanced grid conditions. The proposed control algorithm particularly compensates for the unbalanced grid voltage at the point of common coupling in a collector bus of offshore wind power system. This control algorithm has been formulated based on the symmetrical components in positive and negative rotating synchronous reference frames under generalized unbalanced operating conditions. Instantaneous active and reactive power are described in terms of symmetrical components of measured grid input voltages and currents. Negative sequential component of ac input current is injected to the point of common coupling in the proposed control strategy. The amplitude of negative sequential component is calculated to minimize the negative sequential component of grid voltage under the limitation of current capability in a voltage source converter. The proposed control algorithm makes it possible to provide a balanced voltage at the point of common coupling resulting in the generated power of high quality from offshore wind power system under unbalanced network conditions.

• Microbiology and Biotechnology Letters
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• v.30 no.2
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• pp.151-156
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• 2002

This study was performed to obtain the thermostable $\beta$-glycosidase producing bacteria from hot spring of volcanic area at Atagawa in Japan. KNOUC 202 was selected because it showed thermostable $\beta$-glycosidase activity in sodium phosphate buffer(pH 6.8) at $70^{\circ}C$ for 4h, and it was identified. The strain was aerobic, asporogenic bacilli, immobile, gram negative, catalase positive, oxidase positive, and pigment-producing. Optimum growth was at $70~72^{\circ}C$, pH 7.0~7.2, and it could grow in the presence of 3% NaCl. The main fatty acids in cell were iso-15:0 and iso-l7:0. 16S rRNA sequence of KNOUC 202 showed 99.9% similarity with that of Thermus thermophilus ATCC 27634(HB8). Based on morphological, physiological, biochemical characteristics, cellular fatty acids profile and 16S rRNA sequence analysis, KNOUC 202 was identified as Thermus thermophilus.

• Korean Journal of Child Studies
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• v.19 no.2
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• pp.133-146
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• 1998

This study was designed to assess the developmental sequence of children's understanding of social interaction and to test the effects of support conditions and behavioral domains on the understanding of social interaction. The subjects were one hundred 4- to 8-year-old children. The method was a doll play situation, composed of three different support conditions. Scalogram analysis was used to test the developmental sequence, and ANOVA and paired t-test were used to test the significance of differences in stages. The results of this study evidenced a sequential pattern in the 4- to 8-year-old children's understanding of social interaction. There were also significant differences between stages in the understanding of social interaction according to support conditions and behavioral domains. Higher levels of support produced higher stages of understanding and the understanding of positive social interactions were higher than those of negative social interactions at ages 4 and 5.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.810-812
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• 2003

The dynamic characteristics of a three phase induction motor which is applied by various unbalanced voltages are simulated and analyzed in this paper. The voltage equations and torque equation in the dq stationary reference frame for the three phase star connected induction motor are used in this analysis. MATLAB and SIMULINK are employed as a simulation tool. The dynamic characteristics of speed and torque are simulated for various unbalanced voltages, that is, (1) cases with the same unbalance voltage factor but different unbalanced voltages, (2) cases with only one unbalanced voltages but different degree of unbalance, and (3) cases with the same positive sequence voltage but different negative sequence voltages. The simulated results are compared and analyzed with each other, and also with the results of balanced voltage.

• BMB Reports
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• v.31 no.6
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• pp.525-535
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• 1998

A critical step in the faithful translation of genetic information is specific tRNA recognition by aminoacyl-tRNA synthetases. These enzymes catalyze the covalent attachment of particular amino acids to the terminal adenosine of cognate tRNA substrates. In general, there is one synthetase for each of the twenty amino acids and each enzyme must discriminate against all of the cellular tRNAs that are specific for the nineteen noncognate amino acids. Primary sequence information combined with structural data have resulted in the division of the twenty synthetases into two classes. In recent years, several high-resolution co-crystal structures along with biochemical data have led to an increased understanding of tRNA recognition by synthetases of both classes. The anticodon sequence and the amino acid acceptor stem are the most common locations for critical recognition elements. This review will focus on acceptor stem discrimination by class II synthetases. In particular, the results of in vitro aminoacylation assays and site-directed and atomic group mutagenesis studies will be discussed. These studies have revealed that even subtle atomic determinants can provide signals for specific tRNA aminoacylation.

• Proceedings of the KIEE Conference
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• 2002.07a
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• pp.145-147
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• 2002

In this paper, the improved fault locating method using distributed parameter which calculating the reduced voltage and current according to the ground capacitance in long transmission line was proposed. For the purpose of the fault locating algorithm non influenced source impedance, the loop method was used in the system modeling analysis. To enhance the fault locating, zero sequence of the fault current which is variable according to ground capacitance was not used but positive and negative sequence. System model was simulated using EMTP software. To verify the accuracy of proposed method, in different cases 64 sampled data per cycle was used and 160km and 300km long transmission line has fault resistance $0{\Omega}\;and\;100{\Omega}$ respectively was compared.