• Korean Journal of Veterinary Research
• /
• v.39 no.1
• /
• pp.27-33
• /
• 1999

In the mammalian ovary, follicular development and atresia continuously occur during the reproductive cycles. Follicular atresia occurs through granulosa cell apoptosis. Apoptosis is known as the physiological cell death, which is regulated by bcl-2 gene family. In the bcl-2 gene family, bcl-2 and bcl-xLong are known as inhibitors of apoptosis, whereas bax and bcl-xShort are known as inducer of apoptosis. We thought that bcl-2 protein is associated with follicular development and atresia. But it is not known that the distribution of cells containing bcl-2 protein during follicular development and atresia. Therefore, to examine the distribution of cells with bcl-2 protein during ovarian follicular development and atresia, the immunohistochemistry was used in the rat ovary. Bcl-2 immunoreactivity was localized in the interstitial cells, theca externa cells and granulosa cells around of antrum. All positive signals were observed in the cytoplasm of these cells. Positive signals were strongly observed in the interstitial and theca externa cells of growing antral follicles. While, positive signals were weakly observed in these cells from atretic antral follicles. Positive signals were very weakly observed in the granulosa cells of growing and atretic antral follicles. According to these data, we suggested that bcl-2 proteins which were strongly expressed in the interstitial cells and theca externa cells of growing antral follicles inhibit follicular atresia. And we purposed that bcl-2 proteins regulated follicular development and atresia through the action of bcl-2 gene family.

• The Korean Journal of Health Service Management
• /
• v.5 no.1
• /
• pp.87-99
• /
• 2011

The aim of the research is to figure out how the perception and the attitude of hospital staff have effect on cost management behavior, to check the mediation effect in the relationship between cost attitude and cost management behavior, and to provide some basic data of efficient cost management means for hospital staff. The major results of research can be summed up as following. Subordinate factors of cost perception such as conviction, information, and thought were all revealed as meaningful effect ones on cost attitude, but they didn't have meaningful effect on cost management behavior. However, if we check the final results of direct effect, indirect effect, and total effect in its variants, we can understand that cost perception have indirect effect on cost management behavior via cost attitude. Cost attitude was revealed as the greatest variant having direct effect on cost management behavior. As a conclusion, it seems that the correct cost perception can make positive cost attitude, by which desirable cost management behavior can be induced. Therefore, as the correct cost perception can make positive attitude, it is necessary to construct effective system to provide useful cost information for staff at anytime and at any place continually on organization aspect. It is important to get correct cost perception for the positive attitude of staff. Nevertheless, if the positive attitude would not be formed, it is necessary to search for the ways for personal motivation, for the management behavior cannot emerge positively.

• Journal of People, Plants, and Environment
• /
• v.23 no.3
• /
• pp.321-332
• /
• 2020

Background and objective: The concept of 'money' in the numbers and operations domain is a fundamentally necessary domain of economic life. This study was conducted to examine the effects of a horticulture-mathematics integration program on mathematical attitude and money calculating ability of high school students with intellectual disabilities. Methods: We analyzed the changes in the mathematical attitude and money calculating ability of students with mild intellectual disabilities in S special school in the city of D, Republic of Korea, with 12 students in the control group and 12 students in the experimental group, from August 27 to October 29, 2019. Results: The results of the comparison showed no statistically significant changes in the three items of mathematical attitude for the control group, while the experimental group, which took part in the horticulture-mathematics integration program, showed statistically significant differences across all three items, such as self-concept about the subject (p = .003), attitude toward the subject (p = .004), and study habit related to the subject (p = .012). The horticulture-mathematics integration program, which was developed by integrating horticultural activities and the mathematics curriculum, used plants and horticultural activities to provide students with positive experiences in mathematics. These included the sense of closeness, curiosity, interest, attention, and enjoyment, leading to positive changes in mathematical attitude. In terms of money calculating ability, both the control group and experimental group showed statistical differences across the three items, but the experimental group showed greater degrees of increase, 15.0 or more, in the scores compared to the control group. Conclusion: These results suggest that utilizing horticultural materials as a part of purchase learning programs with elements of money calculation chapters in the mathematics curriculum could lead to the improvement of students' ability in money calculation. These positive changes are thought to be related to the high degrees of interest in horticulture among students, which led to active participation in the program and enabled the simple and repeated purchase activities in the program to generate positive changes in the money calculation ability of the students.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.22 no.4
• /
• pp.173-186
• /
• 2009

Purpose: 1. To analyze the satisfaction and cognition of postpartum care and to aid the spread of oriental medicine in postpartum care. 2. Get a basic guideline of O.M.D's postpartum care. Methods: We studied the satisfaction and cognition of postpartum care with questionnaire from January 2009 to August 2009, who was treated in ○○ Oriental Medical Postpartum care center during 2 weeks. 61 puerperants answered us. Results: 1. In choosing Postpartum care center, puerperants concerned about new born infants' care(50.8%) and treatment for discomfort after delivery(27.86%). 2. 22 puerperants(36.6%) had a positive view in folk remedy for postpartum care and 34 puerperants(55.73%) thought it would be good to eliminate edema and loose weight. 3. Most of puerperants(80.32%) chose oriental medical postpartum care because they wanted to receive oriental medical treatment. 4. 5 puerperants(8.19%) were very satisfied with oriental medical postpartum care than other type of postparum care which they experienced at first delivery. 47(77.04%) were satisfied, 2(3.27%) were unsatisfied and no one was very satisfied. 5. To the questionnaire about the effect of strength recovery, lochia excretion, edema limination, increasing amount of breastmilk, reducing arthralgia, about 36.8% puerperants showed positive view. 6. The positive view about Acupuncture, cupping, moxibustion, herbal treatment was, respectively, 90.16%, 49.18%, 73.77%, 86.88%. Conclusion: Puerperants were satisfied with oriental medical postpartum care and had positive cognition in oriental medical treatment. There is much capability for extension of oriental medicine in postpartum care. So continuous concern, systemic development and publicity about oriental medical postpartum care are required.

• The Journal of Fisheries Business Administration
• /
• v.42 no.1
• /
• pp.97-112
• /
• 2011

Today, the consumer is more careful in buying goods, invests more time in collecting relevant information to avoid any potential danger, and restricts from potential impulse buying. To react this consumer's carefulness, the seafood brands provide much information including the origin labeling system, the traceability, the food's safety & hygiene. Also the branding by region or company is pursued. Like that, a seafood brand's importance is increased, but there lack few researches dealing how current consumer's attitude influences on real purchase behavior, and how the attitude works consumer purchase decision. Therefore, this study researched the brand's influence on the consumer's attitude and purchase intention. For this purpose, this study targeted the salty mackerel and the dried yellow corvina because they are already branded and sold in some popularity, and researched how a brand's popularity, its image, and its recognized quality could effect on the consumer's attitude and purchase intention. As the result, it was appeared that a seafood brand's popularity didn't directly effect on the consumer's purchase intention, but indirectly influenced through the consumer's attitude as a parameter. From this result, improving a seafood brand's popularity needs some time to form the consumer's positive attitude and to lead to consumer purchase intention of seafood brand. So, it is thought that various promotion activities for seafood consumption must be continually performed rather than some temporary special events. Consumers showed more positive attitude on familiar seafood based on a product's original place and the freshness. Also they had better feeling about some seafood with their speciality images rather than the same kinds of products produced in other regions. This attitude temporarily led to purchase intention. Therefore, it is important that the branding strategy development should start from some seafood familiar to us in traditional food culture and food habit, but should delivery the reliance and the freshness in accurately indicating their origins, and should emphasize their differences as specialities. Consumers showed some positive attitudes on the seafood featuring the hygiene, the safety, continual good quality, and their attitudes led to their purchase intentions in temporary. The seafood product reflecting these results the best is the marketing activities on some Andong salty mackerel products acquired HACCP certification. it is thought that a seafood's branding strategy should be established on distinctive branding strategies using reliable certification mark like HACCP based on the hygiene, the safety, and the quality.

• Archives of Pharmacal Research
• /
• v.21 no.3
• /
• pp.298-304
• /
• 1998

Retinoids are applied to not only cancer prevention but also cancer chemotherapy by stimulating differentiation of cells. We studied differentiation inducing effect of all-trans retinoic acid (ATRA) by studying proportion of high dense fractions of stem-like cells and the size of S phase fraction in cell cycle. From mammary organoids obtained from 7- to 8-week old F344 female rat mammary gland, we cultured rat mammary epithelial cells (RMEC) and treated physiological doses of $10^{-6}$, $10^{-7}$, and $10^{-8}$ M ATRA from the first day and then cultured for 4, 7, and 14 days. After that, immunostaining was performed using peanut agglutinin (PNA) and anti-Thy-1.1 monoclonal antibody (Thy-1.1) that can be used as markers of differentiation. We separated four different cell subpopulations by flow cytometry: cells negative to both reagents (B-), PNA-positive cells (PNA+), Thy-1.1-positive cells (Thy-1.1+), and cells positive to both reagents (B+). We observed continuous decreases of high dense fractions of stem-like cells (PNA+ subpopulations) for 14 days and as much decreases as high doses of ATRA, which were thought to be proportional to doses of ATRA. We labeled RMEC with bromodeoxyuridine and investigated cell cycle fractions that went through S phase. We observed a tendency of decrease of S phase fraction with time in culture, which, is thought to be related to continuous decreases of PNA+ subpopulations and inhibitory role of ATRA on cell cycle. These results suggest that physiological doses of ATRA could stimulate differentiation of RMEC and convert stem-like RMEC to differentiated cells in SFM for a relatively long period of 14 days.

• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2002.11a
• /
• pp.118-118
• /
• 2002

Programmed cell death (PCD) is thought as a well-controlled process by which unwanted cells are selectively eliminated. During the last decade many researches have elucidated molecules and their interactions involved in cell death by using largely in vitro induction of cell death or survival signals in a more defined manner, While these critical information and novel findings provide us with clearer understanding of mechanisms underlying cell death, it does by no means explain how PCD occurs and which cells or tissues are affected during normal embryonic development in vivo. In this study, we used zebrafish to examine whether the PCD is occurring selectively or randomly in developing embryos by whole mount in situ TUNEL analysis with specific markers for neural cells. The result revealed that the degree and distribution of TUNEL staining varied considerably throughout gastrulation stage, and there was also a number of TUNEL-negative embryos. Most of TUNEL-positive cells were scattered randomly throughout the blastoderm. During the gastrulation stage about 75 % of the embryos analyzed exhibited more than 5 TUNEL-positive cells. As the dorsal epiblast begins to thicken rather abruptly near the end of gastrulation, TUNEL-positive cells were mainly located along the dorsal side. Although there were some variations in TUNEL staining during segmentation and pharyngeal stages, TUNEL staining continued to be localized to the central nervous system, and was also detected in the sensory organs, trigeminal ganglions, and the primary sensory neurons. High levels of the cell death in developing brain between 20-somite and prim-6 stages are thought to play a role in the morphogenesis and organization of the brain. At prim-16 stage, cell death is considerably reduced in the brain region. Dying cells are mainly localized to the prospective brain region where ectodermal cells are about to initiate neurogenesis. As development progressed, high levels and more reproducible patterns of cell death were observed in the developing nervous system. Intensive TUNEL staining was restricted to the trigeminal ganglions, the primary sensory neurons, and sensory organs, such as olfactory pits and otic vesicles. Thus, PCD patterning in zebrafish embryos occurs randomly at early stages and becomes restricted to certain region of the embryos. The spatio-temporal pattern of PCD during the early embryonic development in zebrafish will provide basic information for further studies to elucidate genes involved in. regulation of PCD largely unknown in vivo during vertebrate embryogenesis.

• Korean Journal of Psychosomatic Medicine
• /
• v.15 no.2
• /
• pp.107-112
• /
• 2007

Introduction : A positive thinking has been known to affect the enhancement of performance. One of temperament characters, harm avoidance is thought to be associated with anxiety related behaviors. Golfer showed highest anxiety and lowest score of harm avoidance, compared to any other sports. We hypothesized that positive feedback and reinforcement in golfer would decrease the anxiety and improve the performance. Methods : Fifty one golfers with the age from 15 to 19 were classified into two groups; 25 golfers who used Zinsser's Changing Negative thoughts to Positive thoughts and Beswick's positive self talk when exercise and competition (P-FB) and 26 golfers who had not (controls). Harm avoidance, Spielberg's Trait and State Anxiety, and Golf scores has been assessed at baseline and 6 months later. Results : There was no significant difference in baseline harm avoidance, anxiety, and golf scores between P-FB and controls. The total golf scores in both groups have improved after 6 month later. However, P-FB group showed greater improvement of total scores and putting scores, compared to controls. The state anxiety in PFB group has been decreased, while there was no change in controls. The change in the state anxiety was correlated with harm avoidance and the change in total golf scores. Discussions : The positive thinking has enhanced performance and state anxiety. The improvement of performance and anxiety in golfers was associated with high harm avoidance. The current study showed that the regulation of anxiety with positive thinking was helpful to the performance enhancement in subject with high harm avoidance.

• Journal of the Korean School Mathematics Society
• /
• v.10 no.4
• /
• pp.455-469
• /
• 2007

In this study, we want to being helpful to improvement of ability to solve mathematical problem, that is grafted on the subjects being able to occur in real life, of students in teaching materials and results studied and developed in the university. For increasing ability to solve ingenious problem and growing in the learning ability of oneself leading of students. The goal of this study is to make possible open research as a result of that students look for problem around real life by one's own efforts and take interest in them through learning mathematics of parents of students, they are the most important fact of educational environment in the mathematics education - earlier than students. In particular, the goal of this study is that students have an positive attitude of mind for mathematics and maximize ability of practical application by the analytic thinking learned through experience of their parents, they survey, analyze and solve problems taken from real life in the method transmitting one's knowledge to others. This study is divided into 2 categories: education of students and education of their parents. By these, we want to disseminate advanced knowledge and theory through students improve the powers of thought, logic and inference, develop ability to solve mathematical problem, stir up motivation of learning and learn knowledge of mathematics become familiar with real life.

• The Journal of Korean Philosophical History
• /
• no.53
• /
• pp.165-190
• /
• 2017

This thesis is for the analysis of the relationships between Mozi's 'Heaven and Man' through the inspection of meaning and emphasis in his three books, 'Heaven's Will', 'Verification of Ghost' and 'Indeterminism'. According to the previous study, the Heaven's Will and Verification of Ghost are justify Mozi's theory of theistic stance with the Heaven's authority. And in case of the 'Indeterminism', there seems to be positive and rational way of awareness and it's thought to be atheistic stance. It is thought that there is a rule of action which is about the personality principle, and it suggest that how the government rule the country, and how the people lead a life. And it is the ghost that give prize or punishment when they did something according to this principle or not. So, men should do their best in actual life to meet the heaven's will. Because my fate is not decided by heaven's order but my own action. And it is thought that the main point of 'the indeterminism' is about Heaven helps those who do independent and active efforts. In short, 'the Heaven's Will', Verification of Ghost' and 'Indeterminism' are neither contradiction nor conversion form theism to atheism. Mozi is thought to divide clearly about ghost's works and people's works, and it is expressed explicitly in his works 'Heaven's Will', 'Verification of Ghost' and 'Indeterminism'.