• Title/Summary/Keyword: Pore ring

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Molecular cloning and sequence and 3D models analysis of the Sec61α subunit of protein translocation complex from Penicillium ochrochloron

  • Azad, Abul Kalam;Jahan, Md. Asraful;Hasan, Md. Mahbub;Ishikawa, Takahiro;Sawa, Yoshihiro;Shibata, Hitoshi
    • BMB Reports
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    • v.44 no.11
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    • pp.719-724
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    • 2011
  • The $Sec61{\alpha}$ subunit is the core subunit of the protein conducting channel which is required for protein translocation in eukaryotes and prokaryotes. In this study, we cloned a $Sec61{\alpha}$ subunit from Penicillium ochrochloron ($PoSec61{\alpha}$). Sequence and 3D structural model analysis showed that $PoSec61{\alpha}$ conserved the typical characteristics of eukaryotic and prokaryotic $Sec61{\alpha}$ subunit homologues. The pore ring known as the constriction point of the channel is formed by seven hydrophobic amino acids. Two methionine residues from transmembrane ${\alpha}$-helice 7 (TM7) contribute to the pore ring formation and projected notably to the pore area and narrowed the pore compared with the superposed residues at the corresponding positions in the crystal structures or the 3D models of the $Sec61{\alpha}$ subunit homologues in archaea or other eukaryotes, respectively. Results reported herein indicate that the pore ring residues differ among $Sec61{\alpha}$ subunit homologues and two hydrophobic residues in the TM7 contribute to the pore ring formation.

Radial Variations of Elements in the Ring-porous Wood (환공재(環孔材) 주요구성요소(主要構成要素)의 방사방향변동(放射方向變動))

  • Park, Sang-Jin
    • Journal of the Korean Wood Science and Technology
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    • v.9 no.3
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    • pp.1-6
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    • 1981
  • This study examined the radial variations in element demensions of mongolian oak (Quercus mongolica.), elm (Ulmus japonica.), maackia(Maackia amurensis.), castor aralia (Kalopanax pictus.) from pith outwards. Disks were cut from the d.b.h.(above 1.2m) of each species and specimen for tangential pore diameters, fiber length, ray height and width were taken from 1-3rd, 5th, 10th, 15th.............40th ring number from pith. The tangential pore diameters in the earlywood increased rapidly near the pith and then maintained almost constant or very gradually increased diameters in about 20th-30th ring number from pith. In the latewood, tangential pore diameters gradually increased from pith to outwards though the increment is smaller than in early wood. Radial variations of ray height and width maintained almost constant, gradually increased or rapidly increased diameters and then maintained almost constant from pith to outwards. Patterns of radial variations in the fiber length were almost coincident with patterns of earlywood pore diameters, and of ray height or width in Ulmus japonica and Maackia amurensis.

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Development and Application of Image Analysis Program for Investigation of Pore Characteristics in Transverse Surface of Hardwoods

  • Kwon, Oh-Kyung;Lee, Phil-Woo
    • Journal of the Korean Wood Science and Technology
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    • v.26 no.2
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    • pp.29-37
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    • 1998
  • An image analysis program with the function of measuring various quantitative characteristics in the transverse surface of wood was developed using Delphi 2.0. Data on pore characteristics (conditions for image processing, proportion of pores in relationship to other elements, tangential diameter, area, tangential and radial diameter, x and y coordinates of pore center, and geometric coefficients) were saved in text file format. In addition, the pore area histogram in the tangential and radial directions was saved as a BMP (bitmap) type file. Analyses indicated that quantitative characteristics such as the relative radial distribution of pores in a growth ring, pore tangential area histogram, and proportion of pore in lumen area appear to be useful in separating four diffuse-porous woods and four ring-porous woods on the species level.

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Lateral Stress and Pore Pressure During One-dimensional Consolidation of Clay (점토의 일차원 압밀과정중 작용하는 수평토압과 간극수압)

  • 김재영
    • Journal of the Korean Geotechnical Society
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    • v.20 no.6
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    • pp.5-10
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    • 2004
  • The earth pressure coefficient at rest for clayey soils in the one-dimensional state, $K_0$ obtained from the triaxial test is not correct in principle because the seepage flow is radial and the displacement of soil elements is three-dimensional. Measurements of the earth pressure and the pore water pressure during one-dimension consolidation in the consolidometer ring are presented. The earth pressure and pore water pressure are measured directly by a circular part of the consolidometer ring of a floating type at its mid height. A plastic clay showed $K_0$=0.5 irrespective of pressure in the consolidometer ring.

Structure and Identification of Ancient Wood (I) -Construction Wood of Yi-dynasty m Jeju Province- (고재(古材)의 구조(構造)와 수종식별(樹種識別)(제(第) 1 보(報)) -제주도지방(濟州道地方)에 있어서 조선시대(朝鮮時代)의 목조건축재(木造建築材)-)

  • Park, Sang-Jin
    • Journal of the Korean Wood Science and Technology
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    • v.6 no.1
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    • pp.15-23
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    • 1978
  • This report observed for anatomical structure and identified species of small wood debris collected at Chungi-Hyankyo, Yunbuk-Chung, Jeju-Hyankyo, Teachung-Hyangkyo and Kwanduk-Chung in Jeju province constructed in a central period of Yi-dynasty, and now named as local cultral assets. 1. Sample of Chungi-Hyangkyo is gymnosperm with window-like pit, axial and radial cannal & dentate ray tracheid. This sample with identified with Pinus densiflora or Pinus thunbergii. 2. Samples of Yunbuk-Chung and Jeju-Hyangkyo are diffuse porous wood having small vessels with scalariform perforation plates scattered almost equally in annual ring, possess heterogenus ray parenchyma & banded apotracheal parenchyma. This sample is identified with Distylium racemosum. 3. Sample of Teachung-Hyangkyo is ring porous wood composed of big vessels of pore zone and compound vessels outside pore zone. It also has simple perforation plates, tylosis, homogenlls uniseriate ray parenchyma & diffuse parenchyma This sample is identified with Castanopsis cuspidata. 4. Sample of kwanduk-Chung is ring porous wood composed of big vessels of pore zone and compound vessels outside pore zone. It has simple perforation plates, heterogenus ray parenchyma, crystal, vasicentric parenchyma. This sample is identified with Zelkova serrata.

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A Fine-scale Half Ring-like Structure around a Pore

  • Song, Donguk;Chae, Jongchul
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.87.2-87.2
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    • 2013
  • We studied a fine-scale half ring-like structure around a pore seen from the high spectral and the high spatial resolution data. Our observations were carried out using the Fast Imaging Solar Spectrograph (FISS) and the InfraRed Imaging Magnetograph (IRIM) installed at the 1.6 meter New Solar Telescope of Big Bear Solar Observatory (BBSO) on 2012 July 19. During the observations, we found a fine-scale half ring-like structure located very close to a pore (~0.4 arcsec apart from the pore). It was seen in the far wing images of the $H{\alpha}$ and Ca II $8542{\AA}$ lines, but it was not seen in the line center images of two lines. The length of the structure is about 4200 km and the width is about 350 km. We determined its line-of-sight velocity using the Doppler shift of the centroid of the Ti II line ($6559.6{\AA}$, close to the $H{\alpha}$ line) and determined horizontal velocity using the NAVE method. we also investigated the magnetic configurations using the Stokes I, Q, U, and V maps of the IRIM. As a results, we found that it has a high blue-shift velocity (~2km) faster than the photospheric features and has a strong horizontal component of the magnetic field. Based on our findings, we suggest that it is associated with small flux emergence, which occurs very close to the pore. Even though it is very small structure, this kind of magnetic configuration can be in chare of the upper chromosphere heating, especially above the pore.

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A Study on the Physical and Mechanical Properties of Some Major Northern Soft woods and Hardwoods (북양산(北洋産) 주요(主要) 침(針), 활엽수재(闊葉樹材)의 재질(材質)에 관(關)한 연구(硏究))

  • Park, Jong-Su;Kim, Su-Chang
    • Journal of Forest and Environmental Science
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    • v.9 no.1
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    • pp.10-18
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    • 1993
  • The study was carried out to investigate the physical (Density, Percentage of latewood, Percentage of pore zone, Mean annual ring width) and mechanical (Compressive strength parallel to the grain, Compression perpendicular to the grain, Shearing strength) properties of some major northern softwoods and hardwoods. The physical and mechanical properties of each species are summerized as Table 2 and the relationship between physical and mechanical factors are discussed. The results of this study were as follows: Density and percentage of latewood are closely related to physical and mechanical properties, but percentage of pore zone and mean annual ring width are remotely related to physical and mechanical properties.

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Mesoporous Silica Catalysts Modified with Sulfonic Acid and Their Catalytic Activity on Ring Opening Polymerization of Octamethylcyclotetrasiloxane (술폰산으로 표면개질된 메조기공 실리카 촉매의 제조 및 Octamethylcyclotetrasiloxane 개환중합에서의 촉매 활성)

  • Lee, Yeonsong;Hwang, Ha Soo;Lee, Jiyoung;Lo, Nu Hoang Tien;Nguyen, Tien Giang;Lee, Donghyun;Park, In
    • Applied Chemistry for Engineering
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    • v.31 no.4
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    • pp.383-389
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    • 2020
  • Mesoporous silica solid catalysts modified with sulfonic acid were prepared for cationic ring-opening polymerization of octamethylcyclotetrasiloxane (D4). Two sets of MCM-41 (1.7 and 2.8 nm) and SBA-15 (8.1 and 15.9 nm) with different pore sizes were used as catalyst supports. The surface of silica materials was modified with (3-mercaptopropyl)trimethoxysilane by silylation reaction and oxidized to sulfonic acid. The structures of the prepared catalysts were examined by X-ray diffraction and nitrogen adsorption-desorption. The pore size, specific surface area, and pore volume of the modified solid catalysts decreased slightly. In addition, the modification of the sulfonic acid on the silica surface was confirmed by using infrared spectroscopy and nuclear magnetic resonance spectroscopy. To observe the effect of the particle size on the catalytic activity, it was observed with a scanning electron microscope. The catalysts were used to synthesize PDMS through a ring-opening polymerization of D4, and the conversion and polymerization rate of the polymerization reaction depended on the pore size, specific surface area, particle size, and particle agglomeration of the catalysts. In order for the polymerization rate, the catalyst prepared with SBA-15 of 8.1 nm pore size had the fastest reaction rate and showed the best catalytic activity.

The Organization of Nanoporous Structure Using Controlled Micelle Size from MPEG-b-PDLLA Block Copolymers

  • Chang, Jeong-Ho;Kim, Kyung-Ja;Shin, Young-Kook
    • Bulletin of the Korean Chemical Society
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    • v.25 no.3
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    • pp.351-356
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    • 2004
  • Selected MPEG-b-PDLLA block copolymers have been synthesized by ring-opening polymerization with systematic variation of the chain lengths of the resident hydrophilic and hydrophobic blocks. The size and shape of the micelles that spontaneously form in solution are then controlled by the characteristics of the block copolymer template. All the materials prepared in this study showed the tunable pore size of 20-80 ${\AA}$ with the increase of hydrophobic chain lengths and up to 660 $m^2$/g of specific surface area. The formation mechanism of these nanoporous structures obtained by controlling the micelle size has been confirmed using both liquid and solid state $^{13}C\;and\;^{29}Si$ NMR techniques. This work verifies the formation mechanism of nanoporous structures in which the pore size and wall thickness are closely dependent on the size of hydrophobic cores and hydrophilic shells of the block copolymer templates.

Preparation and Application of Pore-filled PVDF ion Exchange Membranes (Pore-filled PVDF 이온교환막의 제조 및 응용)

  • 변홍식;박병규;홍병표;여광수;윤무홍;강남주
    • Membrane Journal
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    • v.14 no.2
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    • pp.108-116
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    • 2004
  • In this study, the pore-filled ion-exchange membranes were prepared by using the asymmetric PVDF membrane as a nascent membrane. First, the solution of PVBCI having the chlorornethylate aryl ring of 80 percents and DABCO was made with the mixed solvent of THF and DU (8:2). These mixed solution was then, filled in the pores of PVDF membrane, and left for a day to complete the gelation. Finally the pore-filled anion-exchange membrane is obtained fallowed by the amination of the remaining chloromethyl groups with trimethylamine (TMA, 40 wt% in water) forming the positive ammonium ion sites. This 2 step procedure enabled us to produce the pore-filled membranes without change of size, and to control the properties of final membrane with various degree of cross-linking. The results of SEM and AFM showed the polyelectrolyte existed in the pores of nascent membrane as a certain configuration. From the investigation of the solvent affecting much to the permeability and rejection, it was found that the membranes using mixed solvent of THE and DMF (8:2) showed better performances than the membranes produced by THF only. The result of an investigation for the water permeability of the final membrane at low pressure (100 Kpa) showed a typical ultrafiltration membrane's permeability (8 ∼ 10 kg/$m^2$hr) and good values of rejection (55∼60 percent).