• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.7
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• pp.833-840
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• 2017

For wide application of perilla leaf, which has various healthy functions and can be easily cultured across Korea, the physicochemical and sensory properties of pan bread made with various amounts of squeezed perilla leaf juice (SPLJ) were analyzed. When dough characteristics were analyzed by using farinograph, consistency and dough development time were not significantly different between the control and bread dough made with various amounts of SPLJ, whereas dough stability time increased with increasing SPLJ amount. Expansion rate of dough decreased with increasing SPLJ amount. The volume, specific volume, and baking loss rate of pan bread made with various SPLJ amounts decreased with increasing SPLJ amount. Pan bread crumb colors became thickened and greenish with increasing SPLJ amount. For physical properties of pan bread made with various SPLJ amounts, springiness and cohesiveness decreased with increasing SPLJ amount, whereas brittleness, chewiness, and hardness increased with increasing SPLJ amount. In the sensory strength analysis, pore uniformity and soft texture decreased with increasing SPLJ amount, whereas crumb color (dark greenish), perilla leaf odor, perilla leaf taste, and chewing texture increased with increasing SPLJ amount. In the overall acceptance analysis, 1.5% SPLJ was the most preferred with a score of 7.10. However, statistical differences between 1.5% and 1.0% SPLJ were not detected at P<0.05. In the partial least squares analysis, consumers liked bread with a green crumb color, perilla leaf odor, perilla leaf taste, and soft and chewing texture. In conclusion, physicochemical properties of pan bread made with SPLJ were less desirable than those of the control; however, consumer acceptance of pan bread made with 1.5% SPLJ was shown the highest. Therefore, methods for increasing physicochemical properties of pan bread made with SPLJ need to be developed for wide application of perilla leaf.

• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.173-179
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• 2019

The aim of this study is to investigate the biological activities of Hericium erinaceus. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of H. erinaceus extract was higher than positive control. The inhibitory activities of xanthin oxidase, ${\alpha}$-glucosidase, and hyaluronidase was measured as functional food activity, and inhibitory activities on collagenase, tyrosinase, and astringent effect as beauty food activity in water and ethanol extracts from H. erinaceus. In functional food activity, xanthin oxidase inhibitory activities at $50-200{\mu}g/mL$ phenolic concentration in ethanol extracts from H. erinaceus showed inhibitory activity in dose dependent manner. ${\alpha}$-Glucosidase inhibitory activities at $50{\mu}g/mL$ phenolic concentration showed high activity of higher than 80%. Inhibitory activities on hyaluronidase as anti-inflammation factor showed inhibition effect in dose dependent manner both in water and ethanol extracts. In beauty food activity, Inhibitory activities on collagenase at $200{\mu}g/mL$ phenolic concentration in water and ethanol extracts showed high activity to 65.09 and 58.38% dose dependently. Tyrosinase inhibitory activity in water extract showed 9.4-58.24%. Astringent activity as pore shrink effect in ethanol extracts also showed a very high activity of 18.94-100%. Antimicrobial activity on pathogenic bacteria was highly effective on Staphylococcus aureus, Salmonella enteritidis, Vibrio parahaemolyticus and Escherichia coli at 2.5 mg/mL or above. Therefore, the extracts from H. erinaceus can be used as a functional food and beauty food resources and natural antimicrobial agent on pathogenic bacteria in food.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.121-129
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• 2007

This study was conducted to evaluate the causes and effects of cyanobacterial development and succession in a shallow eutrophic reservoir from March 2003 to February 2004. Phytoplankton succession, sedimentation rate, and sediment composition were analyzed. Algal bioassay also was conducted with the consideration of light, water temperature and nutrients. Cyanobacteria dominated throughout the year, except for spring season (March${\sim}$April) in which diatoms and flagellates dominated. Total cell density increased in July and November when P loading through inflows was high. Oscillatoria spp. and Aphanizomenon sp. were dominant in May and June, respectively, but replaced with Microcystis spp. in July. Thereafter, Microcystis spp. sustained until December, and again shifted to Oscillatoria spp. and Aphanizomenon sp. The dominance of Oscillatoria spp. in May was accompanied with high TN/TP ratio and the increase of water temperature and light intensity. While the dominance of Microcystis spp. was related with relatively low TN/TP ratio, ranging from 46 to 13 (average: 27). The sedimentation rate was highest in March (0.6 m $day^{-1}$) when diatoms dominated. During the period of cyanobacterial dominance, relatively high sedimentation rate was observed in May (0.4 m $day^{-1}$) and October (0.36m $day^{-1}$). C/N ratio of the sediment ranged $6{\sim}8$. Inorganic P concentration in the pore water was low when DO concentration was < 2 mg $O_2$ $L^{-1}$ in the hypolimnion, reflecting the P release from the sediment. Cyanobacterial growth rate depended on phosphorus concentration and water temperature, and high P concentration compensated for the low temperature in the growth rate. Our results suggest that the potential of cyanobacterial development and substantiality in eutrophic reservoirs be high throughout the year, as being supplied with enough P, and emphasize the consideration of sediment man. agement for the water quality improvement and algal bloom control.

• Applied Chemistry for Engineering
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• v.7 no.6
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• pp.1034-1042
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• 1996

Various $TiO_2-SiO_2$ composite powders were prepared by the modified sol-gel method using 1-dodecanol as DCCA (Dryng Control Chemical Additive ). Their characterizations were carried out and their photocatalytic catalysis was examined on the evolution reaction of hydrogen. The weight losses at $500^{\circ}C$ of only $TiO_2$ and $SiO_2$ powders were 33. 0wt% and 42.5wt%, respectively, and those of the $TiO_2/SiO_2$ powders ($TiO_2/SiO_2=25/75$, 50/50 and 75/25) were about $70.0{\pm}3.0wt%$. The released substances from the powders were almost organic matters. The as-prepared powders except only $TiO_2$ powder were amorphous. Transformation of anatase to rutil was hindered by $SiO_2$ component and the crystallinity of anatase was decreased with increasing $SiO_2$ contents. The as-prepared powders were bulky states. By heating at $600^{\circ}C$ for 1 hr $TiO_2-SiO_2$ powders ($TiO_2=100%$, $TiO_2/SiO_2=75/25,\;50/50$) showed agglomerates consisted of particles in submicron, but those of $TiO_2/SiO_2=25/75$ and $SiO_2=100%$ were still bulky states. Specific surface area of the powders heat-treated at $600^{\circ}C$ for 1hr was increased with $SiO_2$ concents and their pore sizes were also depended on $SiO_2$ contents. The photocatalytic activity of $TiO_2/SiO_2=75/25$ heat-treated at $600^{\circ}C$ for 1hr was 0.240mo1/h.g-cat as $H_2$ evolution rate. This value was about 2.0 times that of P-25(Degussa P-25) as a standard photocatalyst.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.329-336
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• 2016

Occurrence of oriental corn borer, Ostrinia furnacalis, and yield in a paddy-upland rotation field for 8 maize (Eolrukchal 1, Heugjeom 2, Miheukchal, Ilmichal, Heukjinjuchal, Chalok 4, Mibaek 2, Daehakchal) and 7 sorghum (Hwanggeumchal, Anzunbaengisusu, Moktaksusu, Sodamchal, DS-202, Nampungchal, Donganme) varieties has been surveyed. In a monitoring study using a pheromone trap carried out from 15 May to 10 September, the density of O. furnacalis adults increased rapidly from about 2 weeks after maize planting and reached the highest density at mid June. After that, their density was fluctuated a little at earlier September. The number of the damaged maize and invasive pores on the stem of 2 maizes and sorghum varieties was examined. The mean number of the damaged maize per 20 plants was 19 and 18 plants, and the number of invasive pores was 1.8 and 1.4 per maize stem in Daehakchal and Mibaek 2, respectively. In a survey carried out at harvest period using 8 maize varieties, the damaged ratio was 94%, 92%, 71%, 64%, 54%, 52%, and 45% in Daehakchal, Mibaek 2, Ilmichal, Eolrukchal 1, Chalok 4, Miheukchal, and Heugjeom 2, respectively. The number of invasive pore per Ilmichal stem was 1.4 and that of the others was less than 1.0 per stem. In addition, the damaged ratio of maize ears was 50% in Ilmichal, 40% in Heukjinjuchal, 37% in Daehakchal, etc. The damage pattern of 2 sorghum varieties, Nampungchal and Donganme, by O. furnacalis larvae was steeply increased from planting to 2 months and the trend was continued up to earlier August. At this time, the mean number of damaged sorghum was 13 and 9.2 plants for Nampungchal and Donganme, and the number of invasive stem pores was 1.06 and 0.46, respectively. In another survey carried out at harvest period for 7 sorghum varieties, their damaged ratio was 95% in DS-202, 76% in Moktaksusu, 75% in Sodamchal, 67% in Nampungchal, 57% in Anzunbaengisusu, 46% in Donganme, and 34% in Hwanggeumchal. The damage of sorghum varieties was much higher and severer than that of maizes by O. furnacalis larvae. The number of invasive pores on a sorghum stem was 1.7 in DS-202, 1.4 in Moktaksusu, 1.3 in Sodamchal, 1.1 in Nampungchal, 1.0 in Anzunbaengisusu, 0.5 in Donganme, and 0.4 in Hwanggeumchal. Meanwhile, there was no distinct connection between damaging results and yields of maizes and sorghums by O. furnacalis larvae in a paddy-upland rotation field. These results from this study can be applicable for the establishment of a management strategy to control Oriental corn borer in paddy-upland rotation fields for maize and sorghum.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.1
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• pp.106-127
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• 2019

This study aims to understand spatio-temporal variations of macrozoobenthos community in Han River (HRE), Geum River (GRE), and Nakdong River estuaries (NRE) of Korea, sampled by National Survey of Marine Ecosystem. The survey was seasonally performed at a total of 20 stations for three years (2015-2017). Sediment samples were taken three times with van Veen grab of $0.1m^2$) areal size and sieved through a 1 mm pore size mesh on site. A total of 1,008 species were identified with 602 species in HRE, 612 in GRE, and 619 in NRE, showing similar number of species between estuaries. Mean density was $1,357ind./m^2$, showing the high in NRE ($1,357ind./m^2$), mid in GRE ($1,357ind./m^2$), and low in HRE ($1,127ind./m^2$). Mean biomass was $116.8g/m^2$, showing similar variations to density ($174.2g/m^2$ in NRE, $129.0g/m^2$ in GRE, $49.0g/m^2$ in HRE). Polychaeta dominated in number of species and density in three estuaries. Biomass-dominated taxon was Mollusca in HRE and GRE, and Echinodermata in NRE. Polychaetous species dominated all three estuaries over 4% of density, such as Dispio oculata, Heteromastus filiformis and Aonides oxycephala in HRE, Heteromastus filiformis and Scoletoma longifolia in GRE, and Pseudopolydora sp. and Aphelochaeta sp. in NRE, showing various density between estuaries. Community structure was determined by various environmental variables among estuaries such as mean grain size and sorting (HRE), salinity and mean grain size (GRE), and salinity, dissolved oxygen, loss on ignition and mud content (NRE). Our study demonstrates the application of different measures to manage ecosystems in three estuaries. HRE needs to alleviate sedimentary stressors such as sand mining, land-filling, dike construction. Management of GRE should be focused on fresh water control and sedimentary stressors. In NRE, monitoring of dominant benthos and process study on hypoxia occurrence in inner Masan Bay are necessary.

• Economic and Environmental Geology
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• v.54 no.4
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• pp.409-425
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• 2021

Carbon dioxide has been known to be a typical greenhouse gas causing global warming, and a number of efforts have been proposed to reduce its concentration in the atmosphere. Among them, carbon dioxide capture and storage (CCS) has been taken into great account to accomplish the target reduction of carbon dioxide. In order to commercialize the CCS, its safety should be secured. In particular, if the stored carbon dioxide is leaked in the arable land, serious problems could come up in terms of crop growth. This study was conducted to investigate the effect of carbon dioxide leaked from storage sites on soil fertility. The leakage of carbon dioxide was simulated using the facility of its artificial injection into soils in the laboratory. Several soil chemical properties, such as pH, cation exchange capacity, electrical conductivity, the concentrations of exchangeable cations, nitrogen (N) (total-N, nitrate-N, and ammonia-N), phosphorus (P) (total-P and available-P), sulfur (S) (total-S and available-S), available-boron (B), and the contents of soil organic matter, were monitored as indicators of soil fertility during the period of artificial injection of carbon dioxide. Two kinds of soils, such as non-cultivated and cultivated soils, were compared in the artificial injection tests, and the latter included maize- and soybean-cultivated soils. The non-cultivated soil (NCS) was sandy soil of 42.6% porosity, the maize-cultivated soil (MCS) and soybean-cultivated soil (SCS) were loamy sand having 46.8% and 48.0% of porosities, respectively. The artificial injection facility had six columns: one was for the control without carbon dioxide injection, and the other five columns were used for the injections tests. Total injection periods for NCS and MCS/SCS were 60 and 70 days, respectively, and artificial rainfall events were simulated using one pore volume after the 12-day injection for the NCS and the 14-day injection for the MCS/SCS. After each rainfall event, the soil fertility indicators were measured for soil and leachate solution, and they were compared before and after the injection of carbon dioxide. The results indicate that the residual concentrations of exchangeable cations, total-N, total-P, the content of soil organic matter, and electrical conductivity were not likely to be affected by the injection of carbon dioxide. However, the residual concentrations of nitrate-N, ammonia-N, available-P, available-S, and available-B tended to decrease after the carbon dioxide injection, indicating that soil fertility might be reduced. Meanwhile, soil pH did not seem to be influenced due to the buffering capacity of soils, but it is speculated that a long-term leakage of carbon dioxide might bring about soil acidification.

• ANNALS OF ANIMAL RESOURCE SCIENCES
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• v.30 no.3
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• pp.111-120
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• 2019

The objective of this study was to investigate the effect of different feed inoculation method on rumen fermentation in an in vitro. Three experimental treatments were used: control (CON, direct dispersion of feed (2 g) in rumen fluid), combinations of direct dispersion (1 g) and nylon bag (DNB, pore size: 50 ㎛, 1 g), and nylon bag (NB, 2 g). An in vitro fermentation experiment was carried out using strained rumen fluid for 48 h incubation time and timothy was used as a substrate. At the end of the incubation, in vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), pH, volatile fatty acids (VFA), ammonia nitrogen (NH₃-N), and microbial community were evaluated and gas production was estimated at 3, 6, 12, 24, 48 h incubation periods. Gas production was higher in CON than DNB and NB at 6 and 12 h incubation time (p<0.01). There were no differences in final gas production, pH, NH₃-N concentration, total VFA production, and VFA profiles among treatments. The IVDMD was lowest in CON (p<0.01) but the IVNDFD was not differed by feed distribution methods. There were no significant differences in general bacteria and fungi. Protozoa count was highest in NB treatment among treatments (p<0.01). The abundance of cellulolytic bacteria, Ruminococcus flavefaciens and Fibrobacter succinogenes, was highest in the CON among treatments (p<0.01).

• Food Science and Preservation
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• v.30 no.2
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• pp.347-357
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• 2023

The succulent plant Aeonium sedifolium leaves contain several compounds that are of interest for their cosmetic uses on the skin. This study measured the inhibitory effects of enzyme production and antioxidant, astringent effects and skin wrinkles using Aeonium sedifolium leaves (ASL). The total phenolics compounds (TPC) content of ASL under optimal extraction conditions was 34.49 mg/g for hot water extract (ASLW) and 61.64 mg/g for 50% ethanol extract (ASLE). The ASLW and ASLE extracts were freeze-dried, powdered, and used as solids. TPC content, 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity, and 2,2'-azinobis (3-ethylben-zothiazoline 6-sulfonate) (ABTS) radical inhibition of the ASL phenolics were tested. The DPPH radical scavenging activities of ASLW and ASLE were tested at a TPC of 100 ㎍/mL. ABTS radical inhibition showed antioxidant activity of 100.00% in ASLW and ASLE, and the antioxidant protection factor of ASLW and ASLE was 1.07 and 1.22, respectively. The thiobarbituric acid-reactive substance (TBARS) inhibitory activity of ASLW and ASLE was 77.00%. The elastase inhibitory activity of ASLE was 69.03%, and collagenase inhibition activity for ASLW and ASLE was 29.82% and 54.76%, respectively. The astringent effect of ASLE was 89.82% at a TPC of 200 ㎍/mL. Thus, we concluded that ASL has the potential as a functional cosmetic ingredient with anti-aging effects on the skin.

• Journal of the Korean Society for Marine Environment & Energy
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• v.16 no.4
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• pp.227-238
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• 2013

The purpose of this study is to develop a process technology to produce high hardness drinking water which meet drinking water standard, remaining useful minerals like magnesium and calcium in the seawater desalination process while removing the sulfate ions and chloride ions. Seawater have been separated the concentrated seawater and desalted seawater by passing on Reverse Osmosis membrane (RO). Using Nano-filtration membrane (NF), We were prepared primary mineral concentrated water that sodium chloride were not removed. By the operation of electro-dialysis (ED) having ion exchange membrane, we were prepared concentrated mineral water (Mineral enriched desalted water) which the sodium chloride is removed. We have produced the high hardness water to meet the drinking water quality standards by diluting the mineral enriched desalted water with deionized water by RO. Reverse osmosis membranes (RO) can separate dissolved material and freshwater from seawater (deep seawater). The desalination water throughout the second reverse osmosis membrane was completely removed dissolved substances, which dissolved components was removed more than 99.9%, its the hardness concentration was 1 mg/L or less and its chloride concentration was 2.3 mg/L. Since the nano-filtration membrane pore size is $10^{-9}$ m, 50% of magnesium ions and calcium ions can not pass through the nano-filtration membrane, while more than 95% of sodium ions and chloride ions can pass through NF membrane. Nano-filtration membrane could be separated salt components like sodium ion and chloride ions and hardness ingredients like magnesium ions and calcium ions, but their separation was not perfect. Electric dialysis membrane system can be separated single charged ions (like sodium and chloride ions) and double charged ions (like magnesium and calcium ions) depending on its electrical conductivity. Above electrical conductivity 20mS/cm, hardness components (like magnesium and calcium ions) did not removed, on the other hand salt ingredients like sodium and chloride ions was removed continuously. Thus, we were able to concentrate hardness components (like magnesium and calcium ions) using nano-filtration membrane, also could be separated salts ingredients from the hardness concentration water using electrical dialysis membrane system. Finally, we were able to produce a highly concentrated mineral water removed chloride ions, which hardness concentration was 12,600 mg/L and chloride concentration was 2,446 mg/L. By diluting 10 times these high mineral water with secondary RO (Reverse Osmosis) desalination water, we could produce high mineral water suitable for drinking water standards, which chloride concentration was 244 mg/L at the same time hardness concentration 1,260 mg/L. Using the linked process with reverse osmosis (RO)/nano filteration (NF)/electric dialysis (ED), it could be concentrated hardness components like magnesium ions and calcium ions while at the same time removing salt ingredients like chloride ions and sodium ion without heating seawater. Thus, using only membrane as RO, NF and ED without heating seawater, it was possible to produce drinking water containing high hardness suitable for drinking water standard while reducing the energy required to evaporation.