• Journal of Korean Society of Forest Science
• /
• v.103 no.1
• /
• pp.59-64
• /
• 2014

This study describes analysis of genetic diversity and spatial genetic structure of Korean poplar (Populus koreana Rehder) in Mt. Odae using I-SSR markers. P. koreana is a deciduous broad-leaved tree species that primarily grows in the alpine valleys of China, Russia and North Korea. In South Korea, P. koreana is found limitedly in Gangwon province. Especially, the population in Mt. Odae is located on the southern limit line, its importance is emphasized from the genetic resource conservation perspective. The Shannon's diversity (I=0.230) and the expected heterozygosity (He=0.151) were relatively low as compared with those of other plant species. Spatial autocorrelation analysis using Tanimoto's distance showed that the genetic patch was founded within 400 m. It is suggested that individual trees for ex situ conservation should be sampled with a minimum distance of 400 m between trees.

• Journal of Plant Biotechnology
• /
• v.31 no.1
• /
• pp.49-53
• /
• 2004

Shoot formation was investigated from in vitro cultivation of exotic hybrid poplar (Populus koreana ${\times}$ P. trichocarpa) with a specific stomatal character occurring both upper and lower surface of leaves. Two different explants (stem and leaf segment) of Peace poplar were cultured on half strength Murashige and Skoog (MS) basal medium supplemented with the various concentrations of thidiazuron as a plant growth regulator. Most adventitious shoots were produced from excised ends of stem or mid-veins of leaf segments. The highest average numbers of shoots were 7.1 and 5.3 with the treatments of 0.02mg/L TDZ in both explants of stem and leaf segment. The highest shooting rates were achieved to 83.3％ and 47.6％ with the concentrations of 0.01mg/L and 0.02mg/L TDZ by axillary bud and leaf cultures, respectively.

• Journal of Korean Society of Forest Science
• /
• v.98 no.5
• /
• pp.513-523
• /
• 2009

Many species, cultivars, and hybrids in Populus have been introduced and developed by the scientists of Department of Forest Genetic Resources, Korea Forest Research Institute in Korea. P. alba ${\times}$ glandulosa, P. euramericana, P. nigra ${\times}$ maximowiczii, P. koreana ${\times}$ P. nigra var. italica, and P. davidiana have been studied from many aspects, i.e., genetics, breeding, physiology, propagation, silviculture, biomass production, biotechnology and phytoremediation. These precedent results will provide a sound basis for a newly-arising research on short-rotation coppice as one of renewable resources and phytoremediation plants. It was found that there were many promising varieties and clones for these purposes. However, minute analysis on specific gravity and caloric values for those plants should be followed under several silvicultural and rotational systems.

• Korean Journal of Plant Resources
• /
• v.19 no.4
• /
• pp.447-458
• /
• 2006

The resource plants at the Manduksan and Mireukbong was listed 550 taxa: 107 families, 339 genera, 481 species, 1 subspecies, 65 varieties and 3 forms. Among 550 taxa listed was confirmed 323 taxa of edible plants (58.7%), 384 taxa of medicinal plants (69.8%), 267 taxa of ornamental plants (48.5%) and 268 taxa of the others (48.7%). Based on the list of the rare plants by the Forest Research Institute, 4 taxa were recorded in the studied areas; Lilium distichum (Preservation priority order: No. 159), Tricyrtis dilatata (No. 97), Aristolochia contorta (No. 151), Prunus yedoensis (No. 110) and based on the list of Korean endemic plants, 11 taxa were recorded; Cephalotaxus koreana, Carex okamotoi, Lilium amabile, Populus tomentiglandulosa, Salix purpurea var. japonica, Pseudostellaria multiflora, Prunus yedoensis, Forsythia koreana, Paulownia coreana, Weigela subsessilis, Aster koraiensis. Specific plant species by floral region were total 30 taxa; Prunus yedoensis in class V, Wistaria floribunda in class IV, 3 taxa (Spiraea salicifolia, Acer palmatum, Asperula lasiantha) in class III, 3 taxa (Potentilla dickinsii, Viola tokubuchiana var. takedana, Caryopteris incana) in class II, 22 taxa (Pinus koraiensis, Hosta capitata, Chloranthus japonicus, Salix glandulosa, Juglans mandshurica, etc.) in class I. This site needs urgent countermeasures, because there have increased many man-made damages like planting exotic plants, picking wild plants and soil compaction induced by human trampling.

• Journal of Plant Biotechnology
• /
• v.6 no.1
• /
• pp.15-19
• /
• 2004

The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

• The Korean Journal of Ecology
• /
• v.18 no.2
• /
• pp.255-263
• /
• 1995

Two poplar clones, I-214 (Populus euramericana) and Peace (P. Koreana × P. trichocarpa), were grown for 21 days in growth chambers at different CO₂concentrations (350, 700 and 2,000 μL·L-1). I-214 has stomata responding to environmental conditions in normal ways and Peace has unresponsive stomata to environmental factors including light, ABA, water stress and CO₂. In both plants, elevated CO₂ stmulated the growth of plant parts, especially leaf dry weight. And a CO₂ enrichment of 700 μL·L-1 CO₂ caused increment of net assimilation rate (NAR). The growth responses of these plants to CO₂ enrichment were different especially at high CO₂ condition (2,000 μL·L-1 CO₂). The total dry weight in Peace increased up to 2,000 μL·L-1 CO₂ but not in I-214. A CO₂ enrichment of 2,000 2,000 μL·L-1 CO₂ had little effect on NAR of I-214 but enhanced NAR of Peace. Although it is uncertain whether the different responses to CO₂ enrichment between I-214 and Peace resulted from the different properties of stomatal responses to long-term CO₂ treatment, the decrease in NAR is probably due in part to CO₂-induced stomatal closure in I-214 but not in Peace.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.10 no.2
• /
• pp.44-53
• /
• 2007

The vascular plants of the studied area in the Gyehwado was listed 378 taxa; 99 families, 259 genera, 322 species, 1 subspecies, 50 varieties and 5 forms. In this, Woody plants were 130 taxa (34.4%), Herbaceous plants were 248 taxa (65.6%) of all 378 taxa vascular plants. Pteridophyta were 7 families, 9 genera, 11 taxa (2.9%), Gymnospermae were 4 families, 5 genera, 9 taxa (2.4%) and Angiospermae were 88 families, 245 genera, 358 taxa (97.7%). Angiospermae in this, Monocotyledoneae were 10 families, 45 genera, 60 taxa (16.8%) and Dicotyledoneae were 78 families, 200 genera, 298 taxa (83.2%). Based on the list of the rare plants by the Forest Research Institute, recorded in the studied areas; Phacelurus latifolius (Preservation priority order; No. 194), Tricyrtis dilatata (No. 97), Aristolochia contorta (No. 151) and based on the list of Korean endemic plants, 6 taxa were recorded; Cephalotaxus koreana, Populus tomentiglandulosa, Carpinus coreana, Indigofera koreana, Forsythia koreana, Weigela subsessilis. Specific plant species by floral region were total 30 taxa; Wistaria floribunda in class IV, 5 taxa (Ligustrum ovalifolium, Callicarpa mollis, Lonicera subhispida, etc.) in class III, 24 taxa (Cephalotaxus koreana, Aristolochia contorta, Grewia biloba var. parviflora, etc.) in class I. The naturalized plants in this site were 12 families, 27 genera, 34 species, 2 varieties, 36 taxa and naturalization rate was 9.5% of all 378 taxa vascular plants.

• Journal of Environmental Science International
• /
• v.16 no.10
• /
• pp.1139-1146
• /
• 2007

The vascular plants in the Mt. Manduk was listed 560 taxa composed of 110 families, 345 genera, 488 species, 1 subspecies, 67 varieties and 4 forms. Based on the list of the rare plants by the Forest Research Institute, 4 taxa were recorded in the studied areas; Lilium distichum (Preservation priority order; No. 159), Tricyrtis dilatata (No. 97), Aristolochia contorta (No. 151) and Prunus yedoensis (No. 110). Based on the list of Korean endemic plants, 12 taxa were recorded; Cephalotaxus koreana, Carex okamotoi, Lilium amabile, Populus tomentiglandulosa, Salix purpurea var. japonica, Pseudostellaria multiflora, Prunus yedoensis, Stewartia koreana, Forsythia koreana, Paulownia core ana, Weigela subsessilis and Aster koraiensis. Based on the list of approved for delivering overseas of plants, 7 taxa were recorded; Carex okamotoi, Lilium distichum, Aristolochia contorta, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha and Saussurea seoulensis. Specific plants by floral region were total 32 taxa; Prunus yedoensis in class V, Wistaria floribunda in class IV, 5 taxa (Girardinia cuspidata, Spiraea salicifolia, Acer palmatum, Stewartia koreana, Asperula lasiantha) in class III, 3 taxa (Potentilla dickinsii, Viola tokubuchiana var. takedana and Caryopteris incana) in class II and 22 taxa (Pinus koraiensis, Hosta capitata, Chloranthus japonicus, Salix glandulosa, Juglans mandshurica, etc.) in class I. The naturalized plants in the surveyed sites were 14 families, 36 genera, 44 species, 2 varieties, 46 taxa and naturalization rate was 8.2% of all 560 taxa of vascular plants. Wild plants disturbing ecosystem like Ambrosia artemisiifolia var. elatior have been increasing. Therefore, continuous control and conservation measures are needed on the ecosystem of Mt. Manduk.

• Journal of agriculture & life science
• /
• v.45 no.5
• /
• pp.17-23
• /
• 2011

Growth characteristics and adaptability of 10 poplar clones planted at a reclaimed tidal flat were evaluated. The contents of $Na^+$, $Ca^{2+}$ and $Mg^{2+}$ were 10.0, 3.4 and 1.5 times higher, respectively than those of control although the electrical conductivity(EC) in the soil at the test plantation was low as much as 0.51 dS/m. The contents of organic matter(OM) and total nitrogen(TN) in the soil were 22.9 and 23.0 times lower than those of control. Average survival rate of 10 poplar clones showed 88% at three years after planting. Clones Eco28(Populus euramericana), Dorskamp(Populus deltoides ${\times}$ P. nigra) and I-476(Populus euramericana) showed the best survival rate of 100%. However, clones 97-19(Populus deltoides(Lux) ${\times}$ P. deltoides(Harvard)) and Suwon (Populus koreana ${\times}$ P. nigra var. italica) were relatively lower than other clones. Average height and DBH of all clones were 4.8 m and 3.6 cm, respectively. Clone Dorskamp showed the greatest height and DBH, 5.9 m and 5.0 cm, respectively. Clones 97-19 and Dorskamp showed the least defoliation by stress and visible damage by insects and diseases, whereas clones Suwon and I-476 were the most sensitive at the reclaimed tidal flat. Clone Dorskamp showed the best adaptability at the reclaimed tidal flat, but clone Suwon showed the worst based on survival rate, growth, and visible damages.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.11 no.4
• /
• pp.37-45
• /
• 2008

The vascular plants of the studied area in Nonsan economical forest growing site appeared to be 341 taxa; 81 families, 218 genera, 290 species, 1 subspecies, 42 varieties and 9 forms. Based on the list of Korean endemic plants, 5 taxa existed; Cephalotaxus koreana, Thalictrum actaefolium, Asperula lasiantha, Populus tomentiglandulosa (Planted species), Paulownia coreana (Planted species). Specific plant species by floral region were total 13 taxa; Cirsium setidens in class III, Potentilla dickinsii in class II, Cephalotaxus koreana, Pinus koraiensis (Planted species), Hosta capitata, Juglans mandshurica, Alnus hirsuta, Quercus variabilis, Ulmus parvifolia, Dictamnus dasycarpus, Gwenia biloba var. parviflora, Vaccinium oldhami, Trigonotis icumae in class I. The naturalized plants in this site were 9 families, 19 genera, 20 species, 1 varieties, 21 taxa and naturalization rate was 6.1% of all 341 taxa vascular plants. The communities were classified into Quercus acutissima community, Pinus densiflora community, Quercus variabilis community, Platycarya strobilacea community, Quercus aliena community of natural forests and Pinus rigida community, Metasequoia glyptostroboides community, Larix leptolepis community of artificial forests.