• 대한치과교정학회지
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• 제24권4호
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• pp.933-943
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• 1994

The present study was undertaken to determine the effect of tensile force on DNA and protein biosynthesis in bone cells, and to identify the cell type(s) which primarily respond to external physical force among the heterogenous bone cell populations. As a prerequisite for this study, two bone cell populations which retain fibroblastic and osteoblastic feature were isolated from fetal rat calvaria with sequential enzyme digestion scheme. Tensile force was delivered to each bone cell population by two acrylic resin plates connected with a orthodontic expansion screw during culture period. Rate of DNA and protein synthesis in each bone cell population were assessed by the incorporated radioactivity of $[^3H]-thymidine$ into DNA and $[^3H]-proline$ into fraction of collagenase-digestible protein and noncollagenous protein, respectively. DNA synthesis of osteoblast-like calvarial cell populations was increased significantly by the application of tensile force for 24 hours. In contrast, no alteration in DNA synthesis of fibroblast-like populations could be observed in response to applied force. Tensile force induced the change in protein synthesis of bone cell populations with the same pattern. Total protein and collagen synthesis were increased whithin 24 hours in osteoblast-like populations, but not in fibroblast-like populations by tensile force application. These findings indicate that physical force can affect cellullar activity of the particular cell population, not all cell Populations residing in bone and osteoblasts respond more sensitively than fibroblasts. So osteoblasts can modulate the behavior of other bone cells including osteoclasts by producing several local regulating factors of bone metabolism. In this context, preferential responsiveness of osteoblasts to applied tensile force observed in this study suggests that osteoblasts may play an important role in regulation of physical force-induced remodelling process.

• 환경영향평가
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• 제27권4호
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• pp.394-401
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• 2018

본 연구는 환경영향평가의 생태면적률의 인구, 지역, 사업 유형, 총 사업면적에 따른 경향성을 분석하였다. 생태면적률은 총대지면적 중 생태적 기능이 있는 토지면적의 비율로, 생태계 보존과 개발된 단지에서 생활하는 시민들의 건강과 삶의 질에 중요한 요소이다. 현재 환경영향평가에서는 가중치가 고려된 생태면적과 총 사업면적의 비로 생태면적률을 산출하며, 각 사업 유형 별로 최소 목표 생태면적률을 제시하여 최소한의 생태면적을 확보하도록 하고 있다. 경향성에 대한 평가는 도시의 개발, 산업입지 및 산업단지 조성, 관광단지 개발, 폐기물처리시설 및 분뇨처리시설의 설치 사업 등 총 4가지 사업에 대하여 총 55개의 표본을 근거로 하였다. 본 연구는 경향성을 분석한 결과 인구에 대한 고려가 반영된 척도, 사업빈도와 지역 전체를 고려한 생태면적률 조정, 그리고 최소달성치 달성 여부에 대한 규제 강화를 통해 현 제도를 개선해야 한다는 결론을 얻었다. 본 연구 이후로 더 많은 수의 데이터를 포함한 광범위한 연구와, 구체적 제도개선에 대한 연구가 후속되어야 한다.

• 대한의생명과학회지
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• 제15권1호
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• pp.31-35
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• 2009

High blood pressure (BP) is the most frequent risk factor among metabolic syndrome components. The control of hypertension is very important to prevent the cardiovascular risk in metabolic syndrome. The dysfunction of calcium channel is responsible in the regulation of the vascular muscle contribution to hypertension. Calcium channel, voltage-dependent, P/Q type, alpha-1A subunit (CACNA1A) gene is located in brain and known to control the intracranial hypertension. In this study, we investigate whether the polymorphisms of CACNA1A gene is associated with hypertension. The 49 CACNA1A genotypes were determined using the Affymetrix Genotyping chip array in 92 hypertension and 279 control individuals from a Korean population. Logistic and multiple regression models were employed to analyze the genetic contributions of polymorphisms. Out of 49 polymorphisms, six SNPs (rs12611029, rs16035, rs7259944, rs10419472, rs17777900, and rs4926294) showed a significant association with hypertension in three alternative models (codominant, dominant, and recessive models; P<0.05 after adjusting for age and sex). Our results suggest that the CACNA1A gene may be associated with hypertension in the Korean population.

• Genomics & Informatics
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• 제3권3호
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• pp.74-79
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• 2005

The H19 gene, located at human chromosome 11p15.5, is imprinted in most normal human tissues. However, imprinting is often lost in tumors suggesting H19 is a putative tumor suppressor. We analyzed the single nucleotide polymorphisms (SNPs) of a 16 kb region that includes the H19 gene and its imprinting control region (ICR) in the Korean population. To identify SNPs, we directly sequenced this region in 18 Korean subjects. We identified 64 SNPs, of which 7 were in the exons of H19, 2 were in the introns, 14 were in the 3' intergenic region and 41 were in the 5' intergenic region. Of the 64 SNPs, 21 had not previously been reported and thus appear to be unique to the Korean population. The identified SNPs of H19 in the Korean population may eventually be useful as genetic markers associated with various diseases. In this study, 7 of the 64 identified SNPs were at CTCF binding sites in the ICR and may affect regulation of H19 gene imprinting. Thus, several genetic variations of the H19 gene may be important markers in human diseases that involve genomic imprinting, including cancer.

• 대한한방내과학회지
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• 제29권1호
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• pp.32-41
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• 2008

Background : Monocyte chemoattractant protein-1(MCP-1), one of the CC chemokines, appears to play a significant role in asthma pathogenesis. It was reported that polymorphism in the MCP-1(-2518 A/G promoter) was associated with asthma in Caucasians, but the association of this polymorphism and asthma patients in the Korean population has not yet been clarified. Objective : We investigated the possible association between 2 polymorphisms (-2518 A/G promoter and Cys35Cys) and asthma patients in a Korean population. Materials and Methods : DNA samples were obtained from 86 Korean asthma patients and 270 healthy controls. MCP-1 genomic variants (-2518 A/G promoter and Cys35Cys polymorphism) were detected by PCR-RFLP. Level of MCP-1 was measured by ELISA for each genotype (n=8) (AA, AG, GG) and allele types of -2518 A/G promoter polymorphism for control subjects. Results : The Cys35Cys polymorphism was associated with asthma patients in Korean population [genotype distribution ($X^{2}=16.011$, P<0.001)]. Comparison of the two groups revealed no detectable differences in genotype and allele frequencies of the -2518 A/G polymorphism. Haplotype frequencies analysis revealed significant difference $(X^{2}=51.70$, P<0.001). MCP-1 serum level of subjects with G genotype of -2518 A/G promoter polymorphism was statistically higher than that with AA genotype (P<0.05). Conclusion : Our data indicate that no association exists between the MCP-1 -2518 A/G polymorphism and asthma susceptibility in the Korean population. However, it is noteworthy that the high prevalence of the -2518 G allele in the Korean population suggests a potentially important ethnic variation in the regulation of MCP-1 production. This variation must be considered in gene-association studies in different ethnic populations.

• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 2007년도 추계학술논문발표회 논문집
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• pp.432-437
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• 2007

• 한국조경학회지
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• 제24권1호
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• pp.42-54
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• 1996

Many of urban parks which have been designated for urban planning regulation are not well developed because the priority of investment is inferior to that of other public facilities. Park development is an urgent urban policy and it is hard to decide priority among parks. Therefore, it is necessary to evaluate the priority of park development. Thus the Analytic Hierarchy Process has been introduced because it is suitable for achieving objectivity by collecting the professional opinions. The goals of park development are evaluated by AHP method and the priorities among goals are conservation of natural environment, satisfying the residents' residents' needs, population density, regional pollution, land acquisition cost, ratio of greeneries and facilities within park areas. Priority of park development is decided by the standardization of each individual inventory data and considering of the professional opinions. Among the multi-attribute judgement methods, AHP method may be the most widely used. Despite the critical evaluations of the AHP, such as limitation of the nine-point scale and the rank reversal problem, application of AHP to decide the priority of park development is suitable for actual circumstances.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1661-1677
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• 2006

Rhizobium and related genera are soil bacteria with great metabolic plasticity. These microorganisms survive in many different environments and are capable of eliciting the formation of nitrogen-fixing nodules on legumes. The successful establishment of symbiosis is precisely regulated and requires a series of signal exchanges between the two partners. Quorum sensing (QS) is a prevalent form of population density-dependent gene regulation. Recently, increasing evidence indicates that rhizobial quorum sensing provides a pervasive regulatory network, which plays a more generalized role in the physiological activity of free-living rhizobia, as well as during symbiosis. Several rhizobia utilize multiple, overlapping quorum sensing systems to regulate diverse properties, including conjugal transfer and copy number control of plasmids, exopolysaccharide biosynthesis, rhizosphere-related functions, and cell growth. Genomic and proteomic analyses have begun to reveal the wide range of functions under quorum-sensing control.

• 천문학회보
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• 제39권2호
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• pp.56.1-56.1
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• 2014

Although the growth of structure, as traced by galaxy clusters, has been extensively studied through cosmological simulations and large-scale surveys, the early formation and evolution of their galaxy content, and its relation to the transformation of the host environment, are still somewhat poorly understood. This is particularly true of the processes that give rise to the quiescent galaxy population between z=3 and z=2. Recent discoveries at z~2 are now bridging the gap between the well-established massive clusters of the last 9 Gyr and the high-redshift universe, and new datasets are now giving us access to statistical populations of intermediate-mass structures at this epoch. I will discuss the properties of quiescent galaxies in the most distant confirmed X-ray detected galaxy clusters, their implications for galaxy quenching at high-redshift as well as the regulation of star formation at group scales at z~2.

• Genomics & Informatics
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• 제10권4호
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• pp.234-238
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• 2012

Genetic epidemiology studies have established that the natural variation of gene expression profiles is heritable and has genetic bases. A number of proximal and remote DNA variations, known as expression quantitative trait loci (eQTLs), that are associated with the expression phenotypes have been identified, first in Epstein-Barr virus-transformed lymphoblastoid cell lines and later expanded to other cell and tissue types. Integration of the eQTL information and the network analysis of transcription modules may lead to a better understanding of gene expression regulation. As these network modules have relevance to biological or disease pathways, these findings may be useful in predicting disease susceptibility.