• Development and Reproduction
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• v.11 no.3
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• pp.235-243
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• 2007

Human umbilical cord blood(HUCB) contains a rich source of hematopoietic stem cells, mesenchymal stem cells and endothelial cell precursors. Mesenchymal stem cells(MSCs) in HUCB are multipotent stem cells, differ from hematopoietic stem cells and can be differentiated into neural cells. We studied on transdifferentiation-promoting conditions in neural cells and cholinergic neuron induction of HUCB-derived MSCs. Neural differentiation was induced by addingdimethyl sulphoxide(DMSO) and butylated hydroxyanisole(BHA) in Dulbeco's Modified Essential Medium(DMEM) and fetal bovine serum(FBS). Differentiation of MSCs to cholinergic neurons was induced by combined treatment with basic fibroblast growth factor(bFGF), retinoic acid(RA) and sonic hedgehog(Shh). MSCs treated with DMSO and BHA rapidly assumed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including $\beta$-tubulin III, GFAP and MBP, was markedly elevated during this acute differentiation. The differentiation rate was about $32.3{\pm}2.9%$ for $\beta$-tubulin III-positive cells, $11.0{\pm}0.9%$ for GFAP, and $9.4{\pm}1.0%$ for Gal-C. HUCB-MSCs treated combinatorially with bFGF, RA and Shh were differentiated into cholinergic neurons. After cholinergic neuronal differentiation, the $\beta$-tubulin III-positive cell population of total cells was $31.3{\pm}3.2%$ and of differentiated neuronal population, $70.0{\pm}7.8%$ was ChAT-positive showing 3 folds higher in cholinergic population than neural induction. Conclusively, HUCB-derived MSCs can be differentiated into neural and cholinergic neurons and these findings suggest that HUCB are alternative cell source of treatment for neurodegenerative diseases such as Alzheimer's disease.

• Ecology and Resilient Infrastructure
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• v.4 no.2
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• pp.75-82
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• 2017

This study was executed to know the effect of the habitat size on huanren brown frog (Rana huanrensis) larvae's growth. We've conducted the study on Mt. Surak in Sanggye-dong, Nowon-gu, Seoul. Our study site was discriminated the huanren brown frog larvae's habitat into the three different areas, such as small area (S1, $3.91m^2$), middle area (S2, $4.42m^2$), and large area (S3, $38.37m^2$) in the same water channel. We measured two times per a week the 15 huanren brown frog larvae's body length, and developmental stages (foreleg, hind leg, tail length) including the environmental factors (humidity, air temperature and water temperature) of the study site from March 30 to June 28 in 2016. The only hatching rate was measured for the three egg sacs at the S2 study site. We found that the average hatching rate was $76.84{\pm}18.23(%)$ at S2. Generally, because of less precipitation during our study periods, all habitat areas for our study site were gradually decreased. The middle area, S2, was not reduced, the small area, S1, was reduced more than a fourth, and the largest area, S3, was drastically reduced more than half from initial area. There was no statistically difference among the three study sites for the three environmental factors (average humidity, average air temperature, and average water temperature) by F-test, but there were significant difference among the three site for the larvae's body length (F-test p< 0.05). Therefore, the larger of habitat areas, the larger of the larvae length as well as the faster of the larvae's developmental stages. When we measured the body lengths of immature huanren brown frogs, the immature frog of S1 was smallest, the immature frog of S3 was middle lenght, and the immature frog of S2 was largest. There were statistically different among the three study sites for the immature huanren brown frogs (F-test, p< 0.05). Because the S2 study site was relatively stable without rarely changing the study area, it meant that huanren brown frog was sensitive to habitat areas. Based on the results of this study, if we study on the effect of the altitude on the growth rate of huanren brown frog, it must be helpful in understanding the habitat environment of the population of huanren brown frog.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.6 no.4
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• pp.153-171
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• 2011

The welfare system and the direction of the welfare policy have been unable to escape greatly from the frame of the past against the facts that the external environments of the national economy of the world including Korea have been changing in quick and rapid speed. Such results have caused the issues related with the welfare, economy and society ended in the ideological collision in connection with the goals of the policy, the right agreement between the policies lacked, and the intervention and conflict between the interest group concerning the policy continued. Social policy of Korea in the past had the level of complementing the parts which could not be solved through the growth. Employment creation had been achieved continuously backed up by the high rate of growth. And the low aging level, the young population structure, and the high rate of childbirth had been the structures that made such achievement possible. New economic, social and welfare environment at home and abroad has been requesting new change in welfare policy. Goal of the economic and social policy is to construct the safe economic and social system. And what has been requested has been the formation of the economic and social policy orienting the welfare nation in form of social investment and welfare expansion. Also the direction in strengthening the welfare system of Korea shall have the balance between the protection and activation strength with the necessity of converting to the prevention welfare from the post welfare. Also the public part, market, the 3rd sector and the share of the role of an individual shall be achieved. And what is needed is the achievement of the transfer from the paradigm of residual welfare to the universal welfare. And such improvements of the welfare system will be able to elevate the possible continuity of the system in long term basis through the improvement of the welfare system.

• Journal of the Korean Regional Science Association
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• v.36 no.2
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• pp.13-24
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• 2020

In modern society, the number of one-person households is increasing significantly. In particular, one-person households have rapidly increased around local small and medium-sized cities. This study examines the characteristics of local small and medium-sized cities by factor and cluster analysis. Analysis of variance are applied to the characteristics of one-person household in different local cities to find the relationship between different types of cities and the characteristics of one-person households. As a result of the study, local small and medium-sized cities are classified into growth stagnation cities, industrial leading cities, regional base cities, and population outflow cities. It is also found that there are several different types of local cities based on the characteristics of one-person households. The growth stagnation city is a city where the regional economy is revitalized due to the development of regional industries in the past. One-person households have a small age group in their 30s and 40s, which are the basis of industrial activities. They have a high proportion of older generation living in more than three rooms in their homes. It is necessary to supply long-term public rental housing and share houses for older generation. The leading city of the industry is a city where the local economy is revitalized as workers are concentrated. One-person households are evenly distributed among all age groups, and the apartment occupancy rate is the highest compared to other types. It is necessary to provide happy housing for youth generation and reconstruction or renovation housing of manhood generation. The regional base city leads the regional base function and the regional economy, but it has reduced workers. Many of one-person households are younger than 30 years old and college educated. They are also high rate of unmarried and live at one room as rental houses. It is needed to expand the supply of small houses such as apartments, officetels and rented houses for youth generation. The population outflow city has a slow local economy and a rural residential environment. It is found that the households of one-person households have high rate of bereavement and the age. They live more than four rooms in single-family homes. It is necessary not only to provide welfare housing but also to create a sound residential environment where cultural exchange is possible.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.3
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• pp.197-203
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• 1993

Greenhouse experiments were conducted to avaluate strain competition, nodulation, patterns of nodule occupancy and population changes of Bradyrhizobium sp. strain HCR-46 $str^{r}cep^{r}$ and CB756 $str^{r}rif^{r}$ in the rhizosphere of peanut(Arachis hypogaea L.) under different root temperatures. Inoculated with two strains using seed coating with peat slurry under different root temperatures, population of each strain in the rhizosphere increased with plant growth and multiplication rate of inoculum in the unit weight of root were showed the highest from 10 to 15days after sowing. The multiplication rate of inoculum in the rhizosphere was $28^{\circ}C$>$34^{\circ}C$>$22^{\circ}C$. The density of HCR-46 $str^{r}cep^{r}$ was more increased than that of CB756 $str^{r}rif^{r}$ under $22^{\circ}C$ and $28^{\circ}C$. While the density of two strains showed no difference under $34^{\circ}C$. Inoculated with HCR-46 $str^{r}cep^{r}$ and CB756 $str^{r}rif^{r}$, respectively at 22, 28 and $34^{\circ}C$, nodulation of each strain was dominated in its inoculation portion. Inoculated with the mixture of HCR-46 $str^{r}cep^{r}$ and CB756 $str^{r}rif^{r}$, occupancy rate of HCR-46 $str^{r}cep^{r}$ was dominated over that of CB756 $str^{r}rif^{r}$ at $22^{\circ}C$ and $28^{\circ}C$, but that was similar between them at $34^{\circ}C$. Dry mass, nodulation, nitrogen content per plant and nitrogenase activity showed higher at $28^{\circ}C$ than at $32^{\circ}C$ and $22^{\circ}C$, while those were higher in HCR-46 $str^{r}cep^{r}$ and mixing HCR-46 $str^{r}cep^{r}$ with CB756 $str^{r}rif^{r}$ than in CB756 $str^{r}rif^{r}$.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.299-304
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• 2014

Effects of false seedbed procedures in organic corn field were evaluated as affected by preparation date and method. False seedbed was prepared at May 7, 14, 21, 28 and June 5 with shallow tillage or flame weeding. Weed suppression rate of each treatment was evaluated 3 weeks after sowing. Optimal false seedbed preparation time was between end of May and early in June. Weed suppression rate of early false seedbed preparation was quiet low. Flaming weed treatment was better than shallow tillage. Weed suppression rate by the second treatment was increased approximately 30-60% compared to $1^{st}$ treatment. Weed suppression rate at 3 weeks after sowing was 70-90% by the second false seedbed preparation. The results showed that the false seedbed technique between end of May and early in June preparation has some potential weed control to reduce weed population during crop growth. Therefore late planting time crop such as corn, sorghum and soybean would be possible to apply false seedbed for organic weed control.

• Journal of Animal Science and Technology
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• v.60 no.5
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• pp.14.1-14.12
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• 2018

Background: Sheep production is a major component of the livestock sector in Ethiopia. The country owing to the large population of 30.70 million estimated numbers of sheep in the country and out of which about 72.14% are females, and 27.86% are males with diverse genetic resources. The real value of indigenous breeds was often under-estimated mostly due to their poor appearance and relatively low productivity. Developing countries in most cases opt for exotic breeds to increase animal productivity through crossbreeding or breed substitution without properly investigating the production potential of the indigenous breeds. The main objective of the research was to identify sheep flock composition and structure, farmers' traits of interest and breeding objective of Wollo highland sheep, and their $F_1$ crossbreed progenies. Results: Smallholder farmers' flock synthesized from breeding ewes, breeding rams, pre-weaned ewe lambs, pre-weaned ram lambs, unproductive ewes, castrated and fattened rams, with the percentage coverage of 29.2, 13.3, 15.5, 16.5, 12.4, and 12.5%, respectively. The maximum number of flock size was 289.0 sheep per flock and higher in the third stratum. The off-take rate percentage of the three strata presented as 21.9% in 1st stratum, 12% in the 2nd stratum, and 16.4% in the 3rd stratum and higher off-take rate recorded in the first stratum. Sheep producer's traits of interest ranked by growth rate (first), body size (second) and marketing value was third rank. Communal breeding (random mating), village based controlled breeding, mixed type and private ram controlled breeding practice were comprised of 39.7, 61.7, 52 and 71.3%, respectively. The percentages of ewes per flock composition were presented as 36.5, 27.1 and 25.5%, respectively in the 3rd stratum, 2nd stratum and 3rd stratum in the order of their importance's. Conclusion: Genetic improvement practices at smallholder sheep producers situation was showing promising outcome with indigenous Washera $F_1$ crossbred lambs and which designated for weaning rate, body size, marketing age, age at first lambing, good temperament and large litter size in the order of their rank. The contemporary breeding practice tendency indicated that, reduced flock size to improve flock productivity via crossbreeding practices.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.252-260
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• 2009

Rice is the most important cereal crop not only in supplying the basic staple food for more than half of the world's population but also as a model plant for functional genomic studies of monocotyledons. Although rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5 mg/L L-cysteine, 1 mM sodium thiosulfate, 1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5 mg/L silver nitrate). Co-cultivation temperature ($23.5^{\circ}C$ for 1 day, $26.5^{\circ}C$ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.

• Journal of Korea Foundry Society
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• v.42 no.2
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• pp.83-93
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• 2022

Throughout this research course, we have analyzed the financial situation of more than 2,700 companies using credit evaluation disclosures from 2017 to 2019. The population was gathered based on the certification of Ppuri companies and Ppuri Expertise companies through the Korea National Ppuri Industry Center, accompanied by the NICE credit evaluation index. For the first time in Korea, we wanted to look at growth, profitability, and stability through financial analysis of the Ppuri industry. Through an indepth analysis, we identified operating income (rate), net income (rate), asset size, and debt ratio, along with three years of Ppuri company workers and total sales fluctuations, and looked at the financial structure per capita. In addition, financial status per person was compared by dividing Ppuri companies into six groups by employee size. Groups were 10 or fewer people, 11 to 20 people, 21 to 50 people, 51 to 200 people, 201-300 people, and 300 or more people; single individual companies were excluded for research convenience. Overall, the financial situation of Ppuri companies was judged to be in a very bad downturn, and financial indicators deteriorated over the course of the three years of investigation. In particular, the smaller the number of employees, the greater the financial fluctuations were and the worse the situations were. Among Ppuri companies, the casting industry, which is the technical starting point for the value chain of the industry, was found to also be in a very bad state, with continued workforce declines, total assets and sales reductions at severe levels, and operating income (rate) and net income (rate) also very poor. This is why we need a suitable and feasible policy direction, something that is difficult but must be allowed to develop.

• Journal of Technologic Dentistry
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• v.26 no.1
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• pp.9-34
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• 2004

The present study reviews the license registration status of dental technicians and dentists from 1970 to 2002, the number of technician members of The Korea Dental Technician Association from 1990 to 2003, and the number of dentist members in 2003. It also considers the yearly membership fluctuations, its growth rates, yearly increase/decrease, the rate of dentist to dental technician, regional distribution of association members, regional yearly increase/decrease of the members, and the regional distribution of dentists and dental technicians. The purpose is to identify and predict the problems in the demand/supply of dental technicians and the regional imbalance of manpower distribution. By doing so, this study attempts to propose the appropriate standard for the supply of dental technicians and point out the necessity of making mid- or long-term plans for ensuring the efficiency of manpower supply and balanced regional distribution. The result is as follows: 1. The number of dental technicians and dentists in 2002 has grown 36.79 and 9.27 times from 1970, and 2.20 and 2.05 times from 1990, respectively. It tool 11 years for the twofold increase of dental technicians from 1990, one year faster than the same increase of dentists. The number of dental technicians per a dentist was the lowest in 1972 (0.21 person), and the highest in 2002 (0.86 person). Specifically, the fastest growth was observed for a year from 1983 (0.47 technician per a dentist) to 1984 (0.6). From 1984 to 1986, the ratio grew by 0.1 every year. Summing up the numbers of dentists and dental technicians who passed the national certification examination, this study predicted the number of dental technicians per a dentist to be 0.88 in 2003, and 0.90 in 2004. 2. From 1990 to 2003, the average distribution of dental technician was 71.76% in the metropolitan areas and 28.24% in other smaller regions. The proportion of dental technicians working in the three major cities (Seoul, Busan, and Daegu) was the lowest in 2001 (52.39%) and the highest in 1996 (62.66%). The majority number of dental technicians who practiced in the metropolitan areas (about 70% of the entire population) were in service in the three major cities. 3. Compared with 1990, the number of dental technicians in 2003 grew 2.28 times in the large cities, and 2.05 times in other smaller regions. The yearly growth rate was the highest in 1994 over the previous year: 26.06% in large cities and 17.86% in the other regions. In the large metropolitan cities, the growth rate was the highest in Incheon (5.8 times for 5 years from 1998 to 2003), In the rest of the regions, the increase of dental technicians was the highest in Gyeonggi (6.5 times from 1990 to 2003). 4. In 2003, as much as 73.40% of all the members of the Korea Dental Technician Association were distributed in the large metropolitan cities, and the rest 26.60% were in other smaller regions. Meanwhile, 54.35% of the dentists practiced in the metropolitan cities, and the rest 45.65% were in service at other regions. This result indicates that more dental technicians than dentists are concentrated in large cities. More than half of all the technician members of the Association are distributed in the three major cities (i.e. Seoul, Busan, Daegu). On the other hand, 42.03% of dentist members are in these three cities and 53.97% of them practice in Seoul, Gyeonggi, and Busan. In 2003, the ratio of dentist to dental technician is 1:0.43 in general, while the ratio is 1:0.58 in the metropolitan cities, and 1:0.43 in other regions. In 2003, 33.30% of all the certified dental technicians are the member of the Korea Dental Technician Association, while 66.82% of all the licensed dentists are its members.