• Journal of Mushroom
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• v.13 no.1
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• pp.21-25
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• 2015

This study was carried out to develop optimum sawdust medium for bottle cultivation of Pholiota adiposa. Five kinds of sawdust media were tested. Period of primordial formation was shorter 1-5 days in T3 and in T4 than in other treatments. Total cultivation period was 3-5 days longer in treatments including oak sawdust than in T5, treatment of poplar+rice bran(8:2). Stipe diameter, stipe length, and number of valid stipe were the biggest in T3. Yield of fruiting body was increased 33% in T3 and 12% in T4, respectively compared to T1 as control. Therefore, T3, treatment of oak sawdust+beet pulf+cottonseed meal(5:3:2), would be appropriate for the commercial production of Pholiota adiposa by bottle cultivation.

• Journal of Biosystems Engineering
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• v.35 no.1
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• pp.37-45
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• 2010

Since SRC (Short-term Rotation Coppice) such as poplar and willow can be harvested in three years, they are known to be a potential forest biomass as fuel for a power plant. The production system including transplanting and harvesting is, however, necessary to be mechanized because such a biomass should be handled in a massive volumetric size. A pull-type stem-cutting transplanter was developed in the research as the first step to realize the production of SRC. A needle-like transplanting device pushes a stem-cutting into the prepared soil bed by a pneumatic cylinder, and another device firms soil around a stem-cutting transplanted. Since this is an intermittent operation, it was necessary to develop a zero horizontal velocity mechanism which enabled only the transplanting needle part to continue a zero horizontal movement relative to the ground during the transplanting operation even when the tractor kept moving forward. The 2-row transplanter can transplant stem-cuttings at the rate of 6.5 seconds per row without missing a single attempt. The planting depth and distance were well maintained and controlled. Their CVs were between 2.1~3.4% and 0.87~1.7% for the depth and the distance, respectively. Although, the transplanted stem-cuttings tended to lean outward from the back-view and forward from the side view, they were planted within the range of $3^{\circ}$ from the upright position.

• The Korean Journal of Mycology
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• v.26 no.2 s.85
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• pp.194-199
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• 1998

Pholiota sp. were collected from the forest of Kangwon province (Mt. Samak, Mt. Odae and Kangwon National University Forest Experimentation) from the early of August to the end of October 1997. Among these fungi, Poliota adiposa was selected for culture experiment because it is suitable for edible mushroom. The Optimal temperature for the mycelial growth of Pholiota adiposa was in the range of $28^{\circ}C$ while that of fruit body formation was $15{\pm}1^{\circ}C$. Brown sugar as carbon source and soybean flour as nitrogen source were good for mycelial growth in commercial liquid culture. The fruit bodies of Pholiota adiposa were artifitially produced on plastic bottle including poplar sawdust and rice bran (4:1).

• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.403-409
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• 2005

Mature nodal segments of 2-year-old greenhouse stock plant were cultured on Murashige and Skoog basal medium supplemented with the different kinds and various concentrations of cytokinins to produce multiple shoots in in vitro condition. The most adventitious shoots were produced from excised ends of nodal segments. The highest average number $(24.6\;{\pm}\;4.6)$ of shoots was produced with the combination of BA 1.0mg/L and TDZ 0.1mg/L in MS medium. In addition, several shoots were formed from lenticels of bark cambium with the same treatment. These concentrations promoted high shooting capability upto $94.6\%$ and NAA was the best cytokinin among five different PGR sources.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.131-135
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• 1995

Physiologically modified stem nodes derived from ex vitro and in vivo explants of hybrid aspen (Populus alba L.X P.grandidentata Michx. 'Crandon') were tested for their multiple shoot regeneration capacity using a broad spectrum dosage of cytokinins. Ex vitro derived stem nodes with excised axillary buds at the time of culture produced 11 to 13 multiple shoots on 20 to 30 $\mu$M zeatin containing Woody Plant Medium (WPM) after 6 weeks. Excision of axillary bud sprouts after 2 weeks of culture and culture of the remaining stem nodes on WPM with 1.0 to 2.0 $\mu$M BA or 10 to 30 $\mu$M zeatin produced 13 to 15 and 7 to 8 shoots per explant, respectively, Multiple tiny shoots were produced when in vivo derived stem nodes (on which all leaves were removed) were cultured on WPM with 30 to 50 $\mu$M 2iP or 20 to 50 $\mu$M zeatin. The greatest number of multiple tiny shoot proliferation (32 to 50 shoots per explant) were obtained when the explants were cultured on media containing 20 $\mu$M zeatin. Successful transplanting of these multiple shoots into the greenhouse and/or nursery was achieved.

• Journal of the Korean Wood Science and Technology
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• v.23 no.1
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• pp.54-60
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• 1995

This paper reports on the changes of equilibrium moisture contents and specific surface areas of Poplar wood(Populus euramericana) for various steam explosion treatments. Equilibrium moisture contents(EMC) and specific surfaces of steam exploded woods were measured under the moisture adsorption course at 25$^{\circ}C$, and compared with those of other materials and wood meals. The EMCs of steam exploded wood meal were 1~5% less in comparison with that of wood meal. In the case of delignified steam exploded wood meal and delignified wood meal, the same tendency was appeared too. But absolute values of EMCs for delignified wood meals were larger than those of the wood meal. For the changes of EMC by the steam exploded conditions, the EMC decreased with the increase of the steam explosion pressure. On the other hand, specific surface areas were calculated from BET plots based on amounts of monomolecular vapor adsorption of various wood meals. Specific surface areas of the wood meal and delignified wood meal were 90~145, 34~90($m^2/g$) respectively, and which were greater in comparison with those of steam exploded wood meals and delignified steam exploded wood meals. From these results, it is considered that the amount of water vapor adsorption was decreased by the increase of the crystallinity, effect of heat treatment, and coating by melted lignin in during the steam explosion.

• The Korean Journal of Mycology
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• v.28 no.1
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• pp.1-5
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• 2000

This experiment were carried out to investigate the effect of the rice bran added into spawn various amounts on the cultivation. Our results show that 10 to 20 percent addition of the rice bran as a supplement results in a good mycelial growth and density. However we didn't find a significant variance among the different species of oyster mushroom using poplar sawdust as a medium. When it inoculated spawn with various amounts of rice bran on the medium of rice straw, the mycelial density was increased according to the increase of the added supplement, while there was no significant in the mycelial growth among the treatments. Through the field test it was showed that 15 to 20 percent addition of the supplement results in the highest yield, the shortest days from spawing to pinhead, and the lowest infection rate.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.110-118
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• 2016

We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.

• Journal of the Korean Wood Science and Technology
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• v.24 no.2
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• pp.7-14
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• 1996

이 연구(硏究)는 미국(美國) 남부(南部)의 9개 주(州)에 소재하고 있는 가구공장(家具工場)에 대한 설문조사(設問調査)를 통해 산업구조(産業構造)와 마케팅 전략을 조사(調査) 하였다. 이번 조사 결과를 요약하면 1. 가구공장(家具工場)이 사용하는 원자재(原資材)는 red oak, pine, poplar, maple 등으로 한국 가구공장(家具工場)이 원자재(原資材)의 95% 이상(以上)을 남양재(南洋材)에 의존하고 있는 것과 뚜렷한 차이를 보였다. 2. 가구공장(家具工場)의 68.8%는 주요시장(主要市場)을 자기 주내(州內)에 가지고 있고, 28.8%가 주(州) 밖에 그리고 조사공장(祖師工場)의 2.5%만이 수출(輸出)했다. 아울러 수출(輸出)이나 자기 주외(州外)에 시장(市場)을 가지고 있을 수록 공장규모(工場規模)가 컸다. 3. 주요시장(主要市場)까지의 거리는 50%가 160km 이내(以內)였는데 이것은 활엽수 제재목(製材木) 공장(工場)의 30%가 800km 밖에까지 시장(市場)을 가지고 있는 것에 비해 매우 좁은 시장(市場)을 형성하고 있는 것으로 나타났다. 4. 제품(製品)의 유통(流通)은 직접 소비자(消費者)에게 출하(出荷)하는 것이 3/4로 압도적이었지만, 중간유통기관에 출하(出荷)하는 곳일수록 공장규모(工場規模)도 컸다. 5. 가구산업(家具産業)의 성공에 기여(寄與)하는 요인(要因)으로는 제품품질(製品品質)과 장기적(長期的)인 소비자(消費者)와의 관계(關係) 증진(增進)을 가장 중요한 요인으로 지적하였고, 그 다음으로 기업에 대한 평판(評判)과 높은 소비자(消費者) 서비스를 지적하였다. 반면에 가구산업발전(家具産業發展)의 장애요인(障碍要因)으로는 원자재(原資材)의 품질(品質)과 원료(原料)의 불안정(不安定)한 공급(供給)이 제일 중요하게 인식되어 있었다. 그러나 해외공급자(海外供給者)와의 경쟁(競爭)은 장애요인(障碍要因)으로 거의 인식되고 있지 않았다. 6. 공장(工場)의 확장(擴張)이나 이전(移轉)에 가장 큰 영향을 미치는 요인(要因)으로는 노동력(勞動力) 문제(問題)(생산성(生産性)과 비용(費用))가 가장 중요하게 지적되었고, 그 다음으로 세금구조(稅金構造), 건설비(建設費) 등이 지적되었다.

• The Korean Journal of Mycology
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• v.24 no.2 s.77
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• pp.149-154
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• 1996

Effects of various kinds of sawdusts, supplements and culture conditions on activities of several enzymes such as protease, phenoloxidase and cellulase produced from mycelium of P. ostreatus grown on sawdust medium were studied and the results are as follows; Higher specific activity of these enzymes was observed when oak tree sawdust and poplar tree sawdust were supplemented with rice bran or wheat bran at rate of 30%, 20% and 10% in total volume respectively. Higher total activities of protease, phenoloxidase and cellulase were observed at 70% of the moisture contents of culture media, while lower activity of these enzymes was observed with 40% moisture contents of sawdust culture medium. The pH 4 and 9 of the sawdust media appeared to be optimum pH for the. production of protease while pH 5 and 7 were optimal for the production of phenoloxidase. The pH 6 of the sawdust medium was optimal for the production of cellulase. The optimum incubating temperature for the production of protease, phenoloxidase and cellulase was $25^{\circ}C$. Higher total activities of protease and phenoloxidase were observed when culture medium was added with wood vinegar at the control, and 0.5% for cellulase.