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Shoot Regeneration of Mature Nodal Segments in Poplar (Populus deltoides)  

Yang Hee-Sun (Education Department, National Environment Research Institute, Ministry of Environment)
Kang Hoduck (Forest Biotechnology Lab., Department of Forest Resources, Dongguk University)
Publication Information
Korean Journal of Plant Resources / v.18, no.3, 2005 , pp. 403-409 More about this Journal
Abstract
Mature nodal segments of 2-year-old greenhouse stock plant were cultured on Murashige and Skoog basal medium supplemented with the different kinds and various concentrations of cytokinins to produce multiple shoots in in vitro condition. The most adventitious shoots were produced from excised ends of nodal segments. The highest average number $(24.6\;{\pm}\;4.6)$ of shoots was produced with the combination of BA 1.0mg/L and TDZ 0.1mg/L in MS medium. In addition, several shoots were formed from lenticels of bark cambium with the same treatment. These concentrations promoted high shooting capability upto $94.6\%$ and NAA was the best cytokinin among five different PGR sources.
Keywords
adventitious shoot formation; lenticel; mature nodal segment; Populus deltoides;
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Times Cited By KSCI : 1  (Citation Analysis)
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