• Korean Journal of Food Science and Technology
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• v.23 no.3
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• pp.291-295
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• 1991

In order to obtain the highest productivity of polysaccharide, acetic acid bacteria was used. Several acetic acid bacteria were investigated to the productivity of polysaccharide, an mutant, Acetobacter pasteurianus IFO 13751-5 selected among serveral acetic acid bacteria which can produce the polysaccharide by radiation of ultra-violet ray. Acetobacter pasteurianus IFO 13751-5 was shown 3 fold polysaccharide production than that of its parents. When the Acetobacter pasteurianus IFO 13751-5 was investigated under the condition of carbon source containing 5% sucrose, the highest amount of polysaccharide (45.95 mg/ml) was obtained. The polysaccharide production by Acetobacter pasteurianus IFO 13751-5 was 55.10 mg/ml by using jar fermentor.

• Fisheries and Aquatic Sciences
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• v.2 no.2
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• pp.182-188
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• 1999

Production of extracellular polysaccharide by Monilinia fructigena in B-I medium containing cereals was higher than that in glucose medium. Productivities in B-I medium and glucose medium were 0.7g/l nd 0.2-03g/l respectively. The maximum content of polysaccharide occurred at the rising point from the lowest pH of culture. As the apparent viscosity of the polysaccharide solution increased, the flow Index (m) decreased, and the consistency Index (Kc) also increased. The polysaccharide solution was a typical pseudoplastic fluid. The mycelium was separated from the culture solution by $300\mu m$ mesh-filter and the polysaccharide was precipitated by adding 50% of ethanol (v/v). The amount of the polysaccharide removed from the filtrated solution was 0.45 g/l and the amount adhered to the mycelium was 0.25g/l. In experiments for investigating growth enhancement of rotifer (Brachionus plicatilis) by the polysaccharide, the dose of the polysaccharide was 1mg per 10,000 organisms of rotifer. Maximum specific growth rate of rotifer with feed consisting of sea Chlorella sp. and the polysaccharide was 1.095/day in the batch culture for 10 days. A semi-continuous culture was done for 30 days, the biomass of rotifer could be harvested twice. Maximum specific growth rate with sea Chlorella sp. and the polysaccharide was 0.734/day before the first harvest, and 1.685/day before the second harvest. Productivity was 38 $cells/ml\; \cdot\; day$ with sea Chlorella sp. and the polysaccharide.

• Microbiology and Biotechnology Letters
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• v.16 no.2
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• pp.98-104
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• 1988

A bacterial isolate FRI-33 which produces hydrophillic polysaccharide was identified and its cultural condition was investigated. FRI-33 was identified as Enterobacter agglomerans. The optimum cultural conditions for polysaccharide production were 3$0^{\circ}C$, pH 5.7, using medium composed of glucose 25 g/$\ell$, peptone 2.0 g/$\ell$, yeast extract 0.5 g/$\ell$, KH$_2$PO$_4$ 1.0g/$\ell$, MgSO$_4$.7H$_2$ O 1.0g/$\ell$, CaCO$_3$ 2.5g/$\ell$. The polysaccharide production after 72 hours was 8.41 g/$\ell$. The polysaccharide was composed of galactose (1.0 mole), xylose (1.5 mole), gluconodeltalactone (1.9 mole) and ribose (0.03 mole). The apparent viscosity of 1% polysaccharide solution was 504 mPa.s at 60 rpm and intrinsic viscosity was 45.80 d$\ell$/ g.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.253-257
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• 2003

This study was executed to investgate the effects of medium components on liquid culture in the flask culture of Coriolus versicolor. This work was focused on raising the mycelial growth and the polysaccharide production. In order to optimize the medium, different carbon and nitrogen sources were investgated. Glucose and yeast extract were chosen for the production of mycelia and polysaccharide as carbon and nitrogen sources, respectively, in the flask culture. For the mycelia growth and polysaccharide production, the medium contained glucose 20g/L, yeast extract 6g/L, $KH_2PO_4$ 0.46g/L, $MgSO_4.7H_2O$ 0.5g/L. The liquid culture conditions for the mycelial growth were $27^{\circ}C$, 200rpm and working volume 100mL using 250mL flask.

• Journal of Life Science
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• v.19 no.11
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• pp.1617-1622
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• 2009

Optium conditions for the production of mycelia and extracellular polysaccharide (EXPS) from submerged mycelial culture of Inonotus obliquus and their immunomodulating activities were investigated. The optmium production of mycelia and EXPS from I. obliquus was observed in mushroom complete medium (MCM). The optimum pH, temperature, and agitation speed for the production of mycelia and EXPS were 5.5, $25^{\circ}C$, and 150 rpm, respectively. The culture period for maximum production of mycelia (10.89 g/l) and EXPS (1.25 g/l) in shake flask cultivation was 11 days. The anticomplementary activity of intracellular polysaccharide (INPS) and EXPS form I. obliquus increased in a dose-dependent manner. Lysosomal enzyme activity of EXPS and INPS increased by 2.0- and 2.2-fold at $100{\mu}g/ml$ concentration, respectively, compared to the control group.

• The Korean Journal of Food And Nutrition
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• v.30 no.1
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• pp.96-104
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• 2017

To obtain the immunomodulating polysaccharide from chaga mushroom (Inonotus obliquus sclerotia, IO), crude polysac- charide fractions (IO-M-CP and IO-CP, respectively) prepared from hot-water extract (IO-W) of I. obliquus by EtOH precipitation after MeOH reflux or not. After IO-W was re-dissolved in water followed by EtOH addition in the case without MeOH reflux, EtOH mixture was fractionated into EtOH-soluble (IO-E) and crude polysaccharide (IO-CP). In the meanwhile, MeOH-soluble fraction (IO-M) was separated from IO-W after MeOH reflux. The residue was dissolved in water and was added by EtOH, and then EtOH mixture was also fractionation into EtOH-soluble (IO-M-E) and crude polysaccharide (IO-M-CP). As a result of the macrophage stimulating activity of these fractions, IO-CP and IO-M-CP showed significantly increased cell proliferation and cytokines production than IO-W. Particularly, IO-M-CP promotes the production of IL-12 more than IO-CP. In the splenocytes proliferating activity and intestinal immune system modulating activity through Peyer's patch, both of 2 crude polysaccharide fractions were significantly promoted in cell proliferation and cytokines production than IO-W, and IO-M-CP was more potent than IO-CP in IL-2 production from splenocytes and GM-CSF production ($10{\mu}g/mL$) in Peyer's patch cells. In addition, immunomodulating polysaccharide fractions (IO-M-CP and IO-CP) prepared from IO-W by EtOH precipitation with or without EtOH reflux showed no significant difference in the chemical composition and component sugar. These results suggested that MeOH reflux might exclude low-molecular weight materials from IO-W and consequently increase the immunomodulating activity of IO-M-CP. Therefore, it was confirmed that immunomodulation of polysaccharide prepared from hot-water extract of chaga mushroom was enhanced by fractionation including MeOH reflux and EtOH precipitation.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.816-820
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• 2005

Polysaccharide-based dressings have increasingly become viable alternatives to somewhat less biocompatible and often problematic cotton or viscose gauzes traditionally used for wound dressings. Polysaccharide which is particularly abundunt in Agaricus blazei Murill is known as the bioactive materials. Polysaccharide extracted from Agaricus blazei Murill enhances the recovery of the damaged epidermal tissue and the production of epithelial cell growth factors which are necessary to heal the burn and wound on the epidermal tissue. In this study, the biopolymer delivery system of polysaccharide extracted from Agaricus blazei Murill into epidermal tissue was elucidated using the skin permeating enhancer in vitro. The enhancing effects of various penetration enhancers and some other enhancers on the permeation of ${\beta}-glucan$ were evaluated using Franz diffusion cell. Permeation of polysaccharide in nomal and burn skin was compared. Polysaccharide was studied for the burn and wound healing activity in the epidermal tissue on rats.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.325-327
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• 2000

We developed a on-line gas control system for three gas components and investigated the effect of oxygen, carbon dioxide, and ethylene on polysaccharide production and cell growth in the suspension cultures of Aloe saponaria. Carbon dioxide(5% v/v) enhanced significantly polysaccharide production while the cell growth was not affected.

• Journal of Ginseng Research
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• v.22 no.4
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• pp.324-330
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• 1998

We Previously reported that Polysaccharide Isolated from panax ginseng C. A. Meyer, stimulates murine splenocytes to proliferate and to be cytotoxic against a wide range of tumor cells in MHC non-restricted manner:) Therefore, we examined the cytokine mRNA expression induced by the ginseng polysaccharide in this paper. This study demonstrates that the ginseng polysaccharide stimulates Thl type cytosine expression such as IL-2 and IFNY, and macrophage type cytokine expression such as IL-lc and GM-CSF in a dose-dependent manner at different time: IL-2 mRNA was induced at 30 min, IL-la, GM-CSF mRNA at 3 hr, IFNY at 6 hr after the ginseng polysaccharide treatment. In contrast with these, Th2 type cytokine expression such as IL-4 and IL-5 was not induced. The generation of the ginseng polysaccharide-activated killer cells which was induced at the optimal doses of 50 pEyml was neutralized in the presence of anti-lL-2, anti-lFNy, anti-IL-l ${\alpha}$ antibodies, showing the importance of these cytokines produced by the ginseng polysaccharide. In flow cytometry analysis, the blastogenesis of IgM+ cells was induced on day 3 and the number of Thy 1.21 cells, CD4+ and CD8+ cells was increased on day 5. The ginseng polysaccharide also induced blastogenesis of T cells. In conclusion, the ginseng polysaccharide may have considerable antitumor immunotherapeutic modality by stimulating the cytokine production from Thl cells and macrophage and by proliferating lymphocytes.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.5
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• pp.359-362
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• 2001

The production of an extracellular polysaccharide, EPS-R, from the marine bacterium Hahella chejuensis was investigated at various aeration rates in a batch culture. Higher aeration rate resulted in enhanced EPS production and increased the viscosity of the culture broth. At an aeration rate of 1.5 vvm, EPS-R (12.2 g/L) was obtained with a yield (Y$\_$p/s) of 0.6 from the STN medium after 72 h of cultivation. The H. chejuensis cells changed rod morphology to a short-rod form in the stationary growth phase.