• Elastomers and Composites
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• 제18권3호
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• pp.87-98
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• 1983

Fundamental properties and surface treatment effects of domestic kaolin calcined at higher temperature were studied to develop reinforcing fillers for rubbers. The results obtained are as follows: (i) X-ray diffraction and scanning electron microscopy studies revealed kaolinite as a major constituent of the raw kaolin used in this study. (ii) Physical properties of natural rubber vulcanizates compounded with the calcined kaolin fillers treated with poly(maleic anhydride) and sodium polyphosphate are favorably improved. Particularly, the kaolin filler treated with sodium polyphosphate(designated as PT series) shows excellent physical properties compared with hydrated silica.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.201.2-201
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• 1996

No Abstract, See Full Text

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 1998년도 제38회 춘계학술발표대회
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• pp.43.1-43.1
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• 1998

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2000년도 제29회 국제학술심포지움 및 추계학술대회
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• pp.77-77
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• 2000

• 대한수의학회지
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• 제53권4호
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• pp.211-216
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• 2013

This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.

• 미생물학회지
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• 제43권1호
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• pp.72-75
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• 2007

Escherichia coli에서 RNA 분해와 가공에 있어서 중심적인 역할을 하는 RNase E의 동족체 단백질인 Streptomyces coelicolor RNase ES의 결합 단백질을 항체침전을 이용하여 탐색하였다. 무기인산을 이용하는 polyphosphate kinase와 리보핵산외부분해효소인 polynucleotide phophorylase의 동족체인 GPSI가 RNase ES와 함께 침전되는 것을 확인하였으며, 이는S. coelicolor에도 E. coli RNase E를 매개로 구성되는 다단백질복합체인 degradosome이 RNase ES에 의해 형성될 수 있음을 암시한다. 계통적으로 멀리 떨어진 이 두 세균에서 정제된 polynucleotide phosphorylase 동족체는 시험관에서의 RNA 분해 특성이 서로 유사함을 보였다. 이러한 실험 결과는 RNase ES가 E. coli degradosome과 유사한 기능과 구조를 가진 단백질 복합체를 형성함을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1061-1065
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• 2001

The antibacterial effects of water extracts of Scutellariate Radix (a dried root of Scutellaria baicalensis GEORGI) and its major flavonoid components, Baicalin and Baicalein, on Salmonella typhimurium, a representative enteric pathogen, were studied. Through a Kriby-Bauer disc analysis, the growth-inhibition activity of Scutellariae Radix against. S. typhimurium was found to be compatible with commercial antibiotics, such as ampicillin, chloramphenicol, and streptomycin. In contrast, the growth of a nonpathogenic E. coli strain was unaffercted by Scutellariae Radix. To examine the effect of polyphosphate kinase (ppk), a putative virulence factor, on the antibacterial activity of Scutellariae Radix, the growth profile of a ppk mutant of S. typhimurium was investigated in a tryptic soy broth containing different concentrations of water extracts of Scutellariae Radix. The ppk mutant was able to grow in 6 mg/ml of water extracts of Scutellariae Radix, whereas in 6 mg/ml of water extracts of Scutellariae Radix, whereas the wild-type could not, implying that the inactivation of ppk made S. typhimurium more resistant to the antibacterial activity of Scutellariae Radix. No enhanced resistance was observed in a ppk mutant of S. typhimurium complemented with a ppk expression vector. The attenuation of the virulence by ppk inactivation was also observed in a virulence assay using BLAB/c mice. Neither Baicalin nor Baicalein exhibited any growth-inhibition activity against S. typhimurium. The water extracts of Scutellariae Radix stimulated the transcription of ppk, especially in the early growth-stage of S. typhimurium.

• 한국식품과학회지
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• 제30권5호
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• pp.1064-1069
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• 1998

호주산 밀(Australian Standard White) 제분한 국수용 밀가루(단백질 8.76%,회분 0.380%)에 피로인산나트륨과 과산화수소의 부가 혼합물인 과산화피로인산나트륨을 $0.2%{\sim}0.4%$ 첨가했을 때 파리노그래프의 안정도가 증가하였고, 익스텐시그래프의 신장성은 감소한 반면 신장에 대한 저항도는 증가하였다. 첨가제 0.2 % 농도에서 아밀로그래프의 최고 점도는 대조구보다 130 BU 높았고, 0.1% 증가함에 따라 최고 점도는 80 BU씩 증가하였다. 칼국수 제조시 가수량은 과산화피로인산나트륨(0.3%)의 첨가에 따라 6.45% 증가하였으며, 면대 신장율은 대조구보다 10% 정도 작았으나, 조리성질에는 영향이 없었다. 삶은 국수의 색깔, 텍스쳐와 선호도를 관능평가한 결과 첨가구가 대조구보다 유의적으로 우수하였다. 국수를 $20^{\circ}C$와 $25^{\circ}C$에서 저장했을 때 이취 발생 시기는 저장 온도에 관계없이 첨가구가 대조구보다 7시간 늦게 나타났으며, 이 때의 pH는 약 6.1이었다.

• 한국물환경학회지
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• 제20권5호
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• pp.480-487
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• 2004

This study was conducted to find the effect of electron acceptors on the formation of granular sludge by using four different types of electron acceptors. The phosphorous uptake, denitrification, and sulfate reduction in anoxic modes were simultaneously occured because of the presence of the polyphosphate accumultating organism(PAO) that utilize nitrate and sulfate as an electron acceptor in the anoxic zone. Denitrirying phosphorous removal bacteria(DPB) was enriched under anaerobic/anoxic/aerobic condition with a nitrate as an electron acceptor, and desulfating phosphorous removal bacteria(DSPB) was enriched under anaerobic/anoxic/aerobic condition with a sulfate as an electron acceptor. Polyphosphate accumulating organism(PAO) were enriched in the anaerobic/aerobic SBR. PAO took up acetate faster than DPB and DSPB during the aerobic phase. The sludge with nitrate and sulfate as an electron acceptors grew as a granules which possessed high activity and good settleability. In the anaerobic/aerobic modes, typical floccular growth was observed. In the result of bench-scale experiment, simultaneous reactions of phosphorus uptake, denitrification and sulfate reduction were observed under anoxic condition with nitrate and sulfate as an electron acceptors. These results demonstrated that the anaerobic/anoxic modes with nitrate and sulfate as an electron acceptors played an important role in the formation of the sludge granulation.

• 한국균학회지
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• 제13권3호
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• pp.141-148
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• 1985

이 연구는 효모 세포를 catabolic repression과 derepression시켰을 때, poly-P 합성 및 분해에 직접 관련된 tripoly-Pase와 poly-Pase의 활성도 동태를 조사하여 세포내 인산 대사 조절양상을 해석하고자 하였다. 또 TLC 방법을 통하여 무기 폴리인산을 분석하고, 배양시기 및 인산 첨가배양에 따른 무기폴리인산의 세포내 축적 상태, 세포내 위치, 전환과정을 조사하기 위해 염색을 통한 volutin 과립의 세포학적 관찰을 행하였다. Tripoly-Pase 활성도는 catabolic repression시킨 세포에서 derepression된 세포에 비하여 6배 이상 증가하였으며, poly-Pase 활성도는 산 불용성 무기폴리인산인 poly-P-"B"가 최대 축적량을 나타날 시기에 최대의 활성도를 보였다. Linear poly-P의 분석에서는 tripoly-P가 주로 검출되었고 n>8이상인 poly-P가 다량 검출되었다. catabolic derepression시킨 세포에서 volutin과립의 형성을 세포학적으로 관찰하였을 때, 세포벽에 존재하는 산 불용성 무기 폴리인산이 우선적으로 합성되었으며, 배양함에 따라 세포질, 핵 또는 액포로 전이되었다.