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http://dx.doi.org/10.14405/kjvr.2013.53.4.211

Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant  

Kim, Kiju (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Park, Soyeon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Cho, Youngjae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Kwak, Jeong-Yeon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Kang, Zheng-Wu (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Kim, Eun-Hee (ChoongAng Vaccine Lab.)
Choi, Hwan-Won (ChoongAng Vaccine Lab.)
Won, Ho-Keun (ChoongAng Vaccine Lab.)
Noh, Yun-Hee (ChoongAng Vaccine Lab.)
Hahn, Tae-Wook (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
Publication Information
Korean Journal of Veterinary Research / v.53, no.4, 2013 , pp. 211-216 More about this Journal
Abstract
This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.
Keywords
cross protection; polyphosphate kinase deleted mutant; Salmonella Enteritidis; temperature-sensitive mutant;
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