Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant

Salmonella enterica serovars Enteritidis의 온도감수성 변이주 및 폴리인산키나아제 변이주의 제작과 방어효과

  • Kim, Kiju (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Park, Soyeon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Cho, Youngjae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kwak, Jeong-Yeon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kang, Zheng-Wu (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Eun-Hee (ChoongAng Vaccine Lab.) ;
  • Choi, Hwan-Won (ChoongAng Vaccine Lab.) ;
  • Won, Ho-Keun (ChoongAng Vaccine Lab.) ;
  • Noh, Yun-Hee (ChoongAng Vaccine Lab.) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 김기주 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 박소연 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 조영재 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 곽정연 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 강정무 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 김은희 ((주)중앙백신연구소) ;
  • 최환원 ((주)중앙백신연구소) ;
  • 원호근 ((주)중앙백신연구소) ;
  • 노윤희 ((주)중앙백신연구소) ;
  • 한태욱 (강원대학교 수의과대학 및 동물의학종합연구소)
  • Received : 2013.07.02
  • Accepted : 2013.09.24
  • Published : 2013.12.31
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Abstract

This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.

Keywords

References

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