• Title/Summary/Keyword: Polyhedra

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곤충 핵다각체병 바이러스 다각체 단백질의 소화액에 의한 분해 (Degradation of Polyhedral Proteins of Nuclear Polyhedrosis Viruses in the Gut Juice of Several Lepidopteran Larvae)

  • 진병래;박범석;우수동;김주읍;강석권
    • 한국미생물·생명공학회지
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    • 제21권6호
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    • pp.513-519
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    • 1993
  • The alkaline protease in the polyhedra preparation of Spodoptera litura nuclear polyhedrosis virus was successfully inactivated by heating at 100C for 20 minutes. SDS-PAGE analysis indicated that heat inactivated polyhedra is composed of major proteins of 31kDa and presumptive its polymer protein of 62kDa. However, this polyhedra was converted into several smaller molecular weight proteins when treated with midgut juice, but not by treatment with heat-inactivated midgut juice.

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Studies on Tolerance Level Against Antheraea mylitta Cytoplasmic Polyhedrosis Virus (AmCPV) in Different Eco-races of Tropical Tasar Silkworm, Antheraea Mylitta Drury

  • Singh, G.P.;Zeya, S.B.;Srivastava, A.K.;Ojha, N.G.;Suryanarayana, N.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제17권1호
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    • pp.153-155
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    • 2008
  • Pathogenic infection in tasar silkworm, Antheraea mylitta Drury is common and there is a breed specific response regarding tolerance. Hence, the tolerance level of three eco-races of tasar silkworm viz. Daba, Sarihan and Raily to Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) infection was tested. The survival of Daba, Sarihan and Raily eco-races was significantly different (p<0.05) when challenged with the same concentration ($1{\times}10^5$ polyhedra/ml) of AmCPV. Daba eco-race was more tolerant to the AmCPV infection having higher survival (65.7%) and $LC_{50}$ values (1000893.17 polyhedra/ml) of AmCPV followed by Sarihan eco-race (50.7% survival and $LC_{50}$ value of AmCPV 187203.6168 polyhedra/ml) and Raily eco-race (24.3% survival and $LC_{50}$ value of AmCPV 5176.37 polyhedra/ml). The tolerance level of Daba and Sarihan eco-races against AmCPV was more than two times than Raily eco-race.

두 종류의 다각체 단백질 유전자를 발현하는 유전자 재조합 핵다각체병 바이러스의 특성 (Characterization of Recombinant Baculovirus Ewpressing Polyhedrin Gene of Bombyx mori and Autographa californica Nuclear Polyhedrois Virus)

  • 김우진;우수동
    • 한국잠사곤충학회지
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    • 제38권1호
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    • pp.36-41
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    • 1996
  • AcNPV 와 BmNPV의 배양세포주에서의 동시감염에 의해 선발된 재조합 바이러스 RecS-A6는 그 다각체 외부 형태가 모바이러스와 다를뿐만 아니라 배양 세포주에 따라서도 그 형태에 차이가 있었다. 이러한 다각체의 특징적인 형태가 나타나는 요인을 다각체 단백질 유전자를 중심으로 조사한 결과 RecS-A6는 AcNPV 와 BmNPV의 다각체 단백질 유전자를 모두 갖고 있는 것이 확인되었으며, 또한 RecS-A6의 다각체를 단백질 전기영동하여 분석한 결과 RecS-A6의 다각체를 단백질 전기영동하여 분석한 결과 AcNPV와 BmNPV의 다각체 단백질이 모두 다각체 형성에 이용되었음을 확인할 수 있었다.

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3차원 정보를 이용한 다면체의 물제인식에 관한 연구 (A Study on the Recognition of Polyhedral Object using 3-D Information)

  • 김영일;우동임;백남칠;우광방
    • 대한전기학회논문지
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    • 제38권6호
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    • pp.458-469
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    • 1989
  • 본 논문에서는 투영정보를 이용하여 기지의 다면체의 3차원 위치와 자세를 측정하는 방법을 제안 하였다. 다면체와 그 상 및 그림자 상과의 관계를 해석하였고, 후보위치를 결정하므로서 다면체의 꼭지점의 3차원 정보를 추출하였다. 입력된 다면체 화상은 전처리 과정을 거친후 물체가 존재하는 장면으로 기술되며, 이 장면의 물체와 데이타 베이스에 저장되어 있는 모델과의 상응(Correspondence)을 탐색하므로써 정합을 수행한다. 실험에서 3차원 정보로부터 몇 개의 모델 영역을 선택하고 장면의 영역과 정합을 수행하므로써 물체를 인식하였다. 실험 결과 복잡하게 구성된 다면체일지라도 측정 오차가 3%이내의 높은 정확도를 갖고 있음을 고찰하였으며, 본 논문에서 구현한 인식 시스템은 슬레시홀드(threshoil)값을 잘 선택하므로써 높은 인식 효율울 갖는다는 것을 확인하였다.

Isolation and Characterization of a Lymantria dispar Multinucleocapsid Nucleopolyhedrovirus Isolate in Korea

  • Shim, Hee-Jin;Roh, Jong-Yul;Choi, Jae-Young;Li, Ming-Shun;Woo, Soo-Dong;Oh, Hyun-Woo;Boo, Kyung-Saeng;Je, Yeon-Ho
    • Journal of Microbiology
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    • 제41권4호
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    • pp.306-311
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    • 2003
  • In Korea, a Lymantria dispar multinucleocapsid nucleopolyhedrovirus, LdMNPV-NM, was isolated and characterized from dead L. dispar larvae. The polyhedra of LdMNPV-NM were irregularly shaped with a diameter of $1.62{\pm}0.33{\mu}m$. Numerous virions comprised of the multinucleocapsid were evident in the electron microscopic examination of the polyhedra cross sections. These polyhedra were composed of a major protein of 30 kDa. The restriction enzyme digestion patterns of LdMNPV-NM showed that this isolate had some different fragments from those of the Gypchek LdMNPV isolate, although their overall profiles were similar. The deduced amino acid sequence of the enhancin gene of LdMNPV-NM showed differences when compared to previously reported enhancin genes of other LdMNPV strains. These results suggested that the LdMNPV-NM isolate from Korea was a new NPV strain and had a new enhancin gene.

삼차원 공간에서 두 다면체 사이의 최소거리 계산을 위한 효율적인 알고리즘의 개발 (Development of an Efficient Algorithm for the Minimum Distance Calculation between two Polyhedra in Three-Dimensional Space)

  • 오재윤;김기호
    • 한국정밀공학회지
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    • 제15권11호
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    • pp.130-136
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    • 1998
  • This paper develops an efficient algorithm for the minimum distance calculation between two general polyhedra(convex and/or concave) in three-dimensional space. The polyhedra approximate objects using flat polygons which composed of more than three vertices. The algorithm developed in this paper basically computes minimum distance between two polygons(one polygon per object) and finds a set of two polygons which makes a global minimum distance. The advantage of the algorithm is that the global minimum distance can be computed in any cases. But the big disadvantage is that the minimum distance computing time is rapidly increased with the number of polygons which used to approximate an object. This paper develops a method to eliminate sets of two polygons which have no possibility of minimum distance occurrence, and an efficient algorithm to compute a minimum distance between two polygons in order to compensate the inherent disadvantage of the algorithm. The correctness of the algorithm is verified not only comparing analytically calculated exact minimum distance with one calculated using the developed algorithm but also watching a line which connects two points making a global minimum distance of a convex object and/or a concave object. The algorithm efficiently finds minimum distance between two convex objects made of 224 polygons respectively with a computation time of about 0.1 second.

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경면 다면체의 모서리 추출 (Extracting roof edges of specular polyhedra)

  • 박원식;조형석
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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    • pp.379-382
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    • 1997
  • This paper introduces a new vision technique for extracting roof edges of polyhedra having specularly reflecting surfaces. There have been many previous works on object recognition using edge information. But they can not be applied to specular objects since it is hard to acquire reliable camera images of specular objects. If there is a method which can extract the edges of specular objects, it is possible to apply edge-based recognition algorithms to specular objects. To acquire the reliable edge images of specular objects, scanned double pass retroreflection method is proposed, whose main physical characteristic is curvature-sensitive. This utility of the physical characteristic is motivated by the idea that roof edges can be characterized as local surfaces of high curvature. In this paper, the optical characteristics of double pass retroreflection are discussed and a series of simulation studies are performed to verify and analyze the sensor characteristics. The results from a series of simulations show the effectiveness of the proposed method.

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Synthesis and Characterization of a Strontium Iron Fluoride Hydrated, Sr2Fe2F10(H2O)

  • Kim, Sun Woo
    • 통합자연과학논문집
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    • 제12권4호
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    • pp.101-104
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    • 2019
  • A hydrated strontium iron fluoride, Sr2Fe2F10(H2O) has been synthesized and characterized. The material was synthesized through mild hydrothermal reaction using an aqueous CF3COOH solution. The material exhibits a one-dimensional structure consisting of chains of corner-shared Fe3+F6 octahedra, isolated Fe3+F5(H2O) octahedra, chains of SrF10 polyhedra and isolated SrF8 polyhedra, respectively. Magnetic property measurements on Sr2Fe2F10(H2O) reveals an antiferromagnetic order at TN of ~2.5 K with a Weiss temperature (θ) of -61.51 K.