• Title/Summary/Keyword: Polarized Light

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Development of Source Profiles for Asbestos and Non-asbestos Fibers by SEM/EDX (SEM/EDX를 이용한 석면 및 비석명의 오염원분류표 개발)

  • Choi, Young-A;Lee, Tae-Jung;Kim, Dong-Sool
    • Journal of Korean Society for Atmospheric Environment
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    • v.23 no.6
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    • pp.718-726
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    • 2007
  • There are many varieties of asbestos: chrysotile, crocidolite, amosite, tremolite, actinolite, and anthophylite. These are widely used in construction materials, brake lining, textile, and so on. Even though non-asbestos fibers such as glassfiber and rockwool have manufactured because asbestos causes asbestosis, lung cancer, mesothelioma, etc., some bad effects of non-asbestos have been also reported. PCM (phase contrast microscopy) and PLM (polarized light microscopy) have been used to qualitatively analyze asbestoses. These techniques have serious drawbacks when identifying and separating various asbestoses. Recently scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDX) has been known as an useful tool to analyze airborne particle since it provides physical and chemical information simultaneously. The purpose of the study was to classify both asbestos and non-asbestos fibers and finally to develop their source profiles by using the SEM/EDX. The source profiles characterized by 6 different types of asbestos fibers and 2 types of non-asbestos fibers had been developed by analyzing a total of 380 fibers. Analytical parameters used in this study were length, width, aspect ratio, and shape as physical information, and Na, Mg, Al, Si, K, Ca, Cr, Mn, Fe, and Cu as chemical information. All the parameters were intensively reviewed.

Solubility of Liquid Crystalline Hydroxypropyl Chitin in Organic Solvent (액정성 히드록시프로필키틴의 유기용매에 대한 용해성)

  • Lee, Young Moo;Kim, Seong Soo;Kim, Seon Jeong;Sung, Yong Kiel;Kang, In Kyu;Son, Tae II
    • Applied Chemistry for Engineering
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    • v.4 no.2
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    • pp.403-408
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    • 1993
  • Hydroxypropyl chitin(HPCH) was prepared from chitin by reacting it with propylene oxide. The formation of liquid crystalline character of HPCH was investigated using halogenated organic solvents. Solid state $^{13}C$ NMR spectra for chitin and HPCH confirmed the incorporation of hydroxypropyl moiety. The degree of substitution of HPCH was around 0.8 as detected by elemental analysis. WAXD patterns of chitin and HPCH showed that an incorporation of hydroxypropyl unit in chitin contributed to reducing the crystallinity and enhancing the solubility in organic solvents. Polarized light microscopic pictures of concentrated HPCH solution showed that HPCH formed cholesteric liquid crystalline character at about 25w/v% solution in dichloroacetic acid and 1, 2-dichloroethane. Inherent viscosity of HPCH solution in a mixed solvent showed a transient decrease.

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Physicochemical properties of naked barley starches (쌀보리 전분의 이화학적 성질)

  • Song, Eun;Shin, Mal-Shick
    • Applied Biological Chemistry
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    • v.34 no.2
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    • pp.94-101
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    • 1991
  • Physicochemical properties of waxy and non-waxy naked barley starches were investigated. The starch granules observed by photomicroscope, polarized-light microscope and scanning electron microscope were round. The starch showed a typical A type by X-ray diffraction pattern. Water binding capacity, swelling power and solubility of waxy naked barley starch were higher than non-waxy naked barley starch at the same temperature. Transmittance of 0.1% starch suspension was increased rapidly from $60^{\circ}C$ in case of waxy naked barley starch and from $65^{\circ}C$ in case of non-waxy naked barley starch. Amylogram revealed that the non-waxy naked barley starches had higher initial pasting temperature than waxy naked barley starch. Enthalpy for gelatinization of non-waxy and waxy naked barley starches were 1.12-1.58 and 0.85 cal/g, respectively.

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A Study on Single Cell Polarized Signals Using Polydimethylsiloxane-based Micropatterned Channel System (폴리디메틸실록산 기반 마이크로패턴 채널 시스템을 이용한 단일 세포의 극성 신호에 관한 연구)

  • Suh, Jung-Soo;Lee, Chanbin;Pan, Yijia;Wang, Yingxiao;Jung, Youngmi;Kim, Tae-Jin
    • Korean Chemical Engineering Research
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    • v.58 no.1
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    • pp.122-126
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    • 2020
  • In this study, we produced the micropatterned channel system using polydimethylsiloxane (PDMS) and micromolding in capillaries (MIMIC) technology and evaluated cellular polarity signals through high-resolved imaging at the single-cell level. In cells treated with platelet-derived growth factor (PDGF), three types of key signals in cell migration; phosphoinositide 3-kinase (PI3 K), Rac, and Actin, were strongly activated in the front area compared to the rear region, whereas myosin light chain (MLC) showed no notable activity in the front and rear areas. Our results will, therefore, provide important information and methodology for studying the correlation between cell polarity signals and cell migration under the newly defined microenvironment.

Fabrication of Wavelength Division Demultiplexing Photodetectors Using Quantum Well Intermixing (다중양자우물의 상호 섞임 현상을 이용한 다중파장검출기의 제작)

  • Yeo, Deok-Ho;Yoon, Kyung-Hun;Kim Sung-June
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.37 no.9
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    • pp.1-6
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    • 2000
  • Utilizing impurity free vacancy diffusion (IFVD) method, area selective intermixing of InGaAs/InGaAsP multi-quantum well (MQW) structure was done. After this, wavelength division demultiplexing waveguide type photodetectoers was integrated and measured. It showed large blue shift in bandgap due to intermixing of MQW. Photodetectors are based on typical p-i-n structure and devices having large and small bandgap areas line up linearly. Width of waveguide and length of each photodetector are 20 and 250 ${\mu}m$, respectively, TE/TM polarized light from tunable laser was butt-coupled to the photodetector and spectral response was measured. Photodetectors can demultiplexing 1480 and 1550 nm wavelength.

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Binding Modes of New Bis-Ru(II) Complexes to DNA: Effect of the Length of the Linker

  • Kwon, Byung-Hyang;Choi, Byung-Hoon;Lee, Hyun-Mee;Jang, Yoon-Jung;Lee, Jae-Cheol;Kim, Seog-K.
    • Bulletin of the Korean Chemical Society
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    • v.31 no.6
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    • pp.1615-1620
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    • 2010
  • Bis-[dipyrido[3,2-$\alpha$:2',3'-c]phenazine)$_2$(1,10-phenanthroline)$_2Ru_2$]$^{2+}$ complexes (bis-Ru(II) complexes) tethered by linkers of various lengths were synthesized and their binding properties to DNA investigated by normal absorption and linear dichroism spectra, and fluorescence techniques in this study. Upon binding to DNA, the bis-Ru(II) complex with the longest linker (1,3-bis-(4-pyridyl)-propane), exhibited a negative $LD^r$ signal whose intensity was as large as that in the DNA absorption region, followed by a complicate $LD^r$ signal in the metal-to-ligand charge transfer region. The luminescence intensity of this bis-Ru(II) complex was enhanced. The observed $LD^r$ and luminescence results resembled that of the [Ru(1,10-phenanthroline)$_2$ dipyrido[3,2-$\alpha$:2',3'-c]phenazine]$^{2+}$ complex, whose dipyrido[3,2-$\alpha$:2',3'-c]phenazine (dppz) ligand has been known to intercalate between DNA bases. Hence, it is conclusive that both dppz ligands of the bis-Ru(II) complex intercalate. The binding stoichiometry, however, was a single intercalated dppz per ~ 2.3 bases, which violates the "nearest binding site exclusion" model for intercalation. The length between the two Ru(II) complexes may be barely long enough to accommodate one DNA base between the two dppz ligands, but not for two DNA bases. When the linker was shorter (4,4'-bipyridine or 1,2-bis-(4-pyridyl)-ethane), the magnitude of the LD in the dppz absorption region, as well as the luminescence intensity of both bis-Ru(II) complexes, was half that of the bis-Ru(II) complex bearing a long linker. This observation can be elucidated by a model whereby one of the dppz ligands intercalates while the other is exposed to the aqueous environment.

Morphometric analysis on bone formation effect of $\beta-TCP$ and rhBMP-2 in rabbit mandible (토끼의 하악골에서 $\beta-TCP$ 와 rhBMP-2의 골형성 효과에 대한 형태계측학적 연구)

  • Kim, Kyu-Nam;Yang, Jung-Eun;Jang, Jea-Won;Sasikala, Balaraman;Wang, Beng;Kim, Il-Kyu
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.36 no.3
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    • pp.161-171
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    • 2010
  • Introduction: This study was to assess the effectiveness of new bone formation and regeneration by using a rhBMP-2 and $\beta-TCP$ as a carrier in rabbits’mandible. Materials and Methods: The mandibles of 36 rabbits were exposed and cortical bone was penetrated for this study. The experimental subjects were divided into 3 groups each 12 rabbits ; control group, experimental group 1, and experimental group 2. Control group had the defect itself without any treatment, in the experimental group 1, $\beta-TCP$P only was grafted, and in the experimental group 2, rhBMP-2 soaked in $\beta-TCP$ was grafted. The rabbits were sacrificed after 1, 2, 3, 4, 6, and 8weeks, and new bone formation area was examined and measured for bone quantitative and qualitative analysis with light, fluorescent and polarized microscopy. Results: In the experimental group 1, new bone formation from the adjacent host bone was made by osteoconduction, and in the experimental group 2, direct new bone formation by osteoinduction of rhBMP-2 as well as new bone formation by osteoconduction of $\beta-TCP$ were observed. Conclusion: rhBMP-2 of experimental group 2 is very effective in the bone formation in early 2weeks and bone remodelling from 3weeks.

Effect of Molecular Weights of Polyethyleneimine on the Polyplex Formation with Calf Thymus DNA

  • Cho, Tae-Sub;Jungho Han;Kim, Seog K.;Lee, Jae-Cheol
    • Macromolecular Research
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    • v.12 no.3
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    • pp.276-281
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    • 2004
  • In this study we investigated the spectral properties, including electric absorption, circular and linear dichroism (CD and LD), and fluorescence emission, of DNA in a DNA-branched polyethyleneimine (BPEI) polyplex at various polymer molecular weights (M$\_$w/) and BPEI-amine-to-DNA-phosphate ratios (N/P ratios). All BPEIs exhibited a common N/P dependence in their absorption and CD spectra. At N/P ratios < 1.0, we observed some hyperchromism in the absorption spectrum, red-shifts in CD bands, and decreases in LD intensity and fluorescence intensity of intercalated ethidium. At intermediate N/P ratios, complete collapse of all spectra occurred. As the N/P ratio increased further, the polyplex dissolved in water. From its characteristic CD spectrum obtained under these conditions, we conclude that the DNA exists in a B-like form. The fluorescence and LD intensities never recovered even at high N/P ratios- which indicates that the dissolved polyplex possesses positive charges and the DNA in the polyplex is condensed despite its B-form CD spectrum. The N/P range in which the absorption and CD signals collapsed was wider when the BPEIs M$\_$w/ decreased. In the case where the BPEIs M$\_$w/ was 0.8 k, recovery of the absorption and CD spectral properties at a high N/P ratio was never achieved, which suggests that the molecular weight of the polymer plays an important role in its dissolution at a high N/P ratio.

Design and fabrication of dichroic mirror and broadband H/R mirror for color separation (색분리를 위한 Dichroic미러 및 광대역 고반사 미러의 설계와 제작)

  • 박영준;박정호;황영모;김용훈;이진호;이상학
    • Korean Journal of Optics and Photonics
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    • v.8 no.3
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    • pp.183-188
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    • 1997
  • Cut-off filters would reject all the radiation below and transmit all that above a certain wavelength or vice versa. In this paper, we study design and farbrication of dichroic mirror and broadband high reflective mirror for color separation of white laser beam source to R.G.B color beam source. In laser display system, color separation is very important. We fabricated below specific component for finite color separation of the Kr-Ar laser source. At 45$^{\circ}$ incidence s-polarized light , it is required that - H/R in blue region R>99%, H/T in green and red region T>90% - H/R in green and red region R>99%, H/T in blue region T>90% - H/R in green region R>99%, H/T in red region T>90% We composed the optical system and realize the full color image.

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Friable Test and Risk Assessment for Asbestos in Soils using the Releasable Asbestos Sampler (RAS) (석면 비산 장비를 이용한 토양 중 석면 비산 특성 및 위해성 평가)

  • Kim, In Ja;Kim, Jeong-Wook;Ryu, Hyun Sik;Jung, Myung Chae
    • Journal of Soil and Groundwater Environment
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    • v.17 no.5
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    • pp.68-74
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    • 2012
  • The objective of this study is to examine a releasable asbestos sampler developed for measuring friable asbestos from soil to air controlled by wind velocity and water contents. Asbestos contaminated soil with 3.75% at the Sinsuk-asbestos mine was sampled, air-dried and sieved to 100 mesh, then homogenized during 24 hours' agitation. Various wind velocities, 1 m/s, 2 m/s, 3 m/s, and 5 m/s, were applied to evaluate releasable characteristics of the releasable asbestos sampler. In addition, soils with 1.0%, 3.4%, 4.4%, 11.0%, 16.2%, and 20.0% of water contents were also examined the amount of friable asbestos by the releasable asbestos sampler. Collected soil and air samples were analyzed by polarized light microscopy (PLM) and phase contrast microscopy (PCM), respectively. Those contents were applied to calculate an excess life cancer risk (ELCR). This study also discussed the relationship with risk assessment by a weeding scenario of activity based sampling (ABS) and field applied releasable asbestos sampler. The result was shown that friability of asbestos in soil increased with wind velocity and decreased with water content. In comparison with ELCR results, over 10E-4 of cancer risk was found in condition on < 5% water content and > 3m/s wind velocity.