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http://dx.doi.org/10.9713/kcer.2020.58.1.122

A Study on Single Cell Polarized Signals Using Polydimethylsiloxane-based Micropatterned Channel System  

Suh, Jung-Soo (Department of Integrated Biological Science, Pusan National University)
Lee, Chanbin (Department of Integrated Biological Science, Pusan National University)
Pan, Yijia (Department of Bioengineering, University of California at San Diego)
Wang, Yingxiao (Department of Bioengineering, University of California at San Diego)
Jung, Youngmi (Department of Integrated Biological Science, Pusan National University)
Kim, Tae-Jin (Department of Integrated Biological Science, Pusan National University)
Publication Information
Korean Chemical Engineering Research / v.58, no.1, 2020 , pp. 122-126 More about this Journal
Abstract
In this study, we produced the micropatterned channel system using polydimethylsiloxane (PDMS) and micromolding in capillaries (MIMIC) technology and evaluated cellular polarity signals through high-resolved imaging at the single-cell level. In cells treated with platelet-derived growth factor (PDGF), three types of key signals in cell migration; phosphoinositide 3-kinase (PI3 K), Rac, and Actin, were strongly activated in the front area compared to the rear region, whereas myosin light chain (MLC) showed no notable activity in the front and rear areas. Our results will, therefore, provide important information and methodology for studying the correlation between cell polarity signals and cell migration under the newly defined microenvironment.
Keywords
Polydimethylsiloxane; Micromolding in capillaries; Micropattern; Cell polarity; Cell imaging;
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1 Kamei, K. I., Mashimo, Y., Koyama, Y., Fockenberg, C., Nakashima, M., Nakajima, M. and Chen, Y., "3D Printing of Soft Lithography Mold for Rapid Production of Polydimethylsiloxane-based Microfluidic Devices for Cell Stimulation with Concentration Gradients," Biomedical Microdevices, 17(2), 36(2015).   DOI
2 Dewez, J. L., Lhoest, J. B., Detrait, E., Berger, V., Dupont-Gillain, C. C., Vincent, L. M. and Rouxhet, P. G., "Adhesion of Mammalian Cells to Polymer Surfaces: from Physical Chemistry of Surfaces to Selective Adhesion on Defined Patterns," Biomaterials, 19(16), 1441-1445(1998).   DOI
3 Veevers-Lowe, J., Ball, S. G., Shuttleworth, A. and Kielty, C. M., "Mesenchymal Stem Cell Migration is Regulated by Fibronectin Through ${\alpha}5{\beta}1$-integrin-mediated Activation of PDGFR-${\beta}$ and Potentiation of Growth Factor Signals," J. Cell. Sci., 124(8), 1288-1300(2011).   DOI
4 Pertz, O., Hodgson, L., Klemke, R. L. and Hahn, K. M., "Spatiotemporal Dynamics of RhoA Activity in Migrating Cells," Nature, 440(7087), 1069-1072(2006).   DOI
5 Duchek, P., Somogyi, K., Jekely, G., Beccari, S. and Rorth, P., "Guidance of Cell Migration by the Drosophila PDGF/VEGF Receptor," Cell, 107(1), 17-26(2001).   DOI
6 Yamaguchi, H. and Condeelis, J., "Regulation of the Actin Cytoskeleton in Cancer Cell Migration and Invasion," Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1773(5), 642-652(2007).   DOI
7 Gardel, M. L., Schneider, I. C., Aratyn-Schaus, Y. and Waterman, C. M., "Mechanical Integration of Actin and Adhesion Dynamics in Cell Migration," Annual Review of Cell and Developmental Biology, 26, 315-333(2010).   DOI
8 Lutolf, M. P. and Hubbell, J. A, "Synthetic Biomaterials as Instructive Extracellular Microenvironments for Morphogenesis in Tissue Engineering," Nat. Biotechnol., 23(1), 47-55(2005).   DOI
9 Plotnikov, S. V., Pasapera, A. M., Sabass, B. and Waterman, C. M., "Force Fluctuations Within Focal Adhesions Mediate ECMRigidity Sensing to Guide Directed Cell Migration," Cell, 151(7), 1513-1527(2012).   DOI
10 Ridley, A. J., "Rho GTPases and Cell Migration," Journal of Cell Science, 114(15), 2713-2722(2001).   DOI
11 Rice, J. J., Martino, M. M., De Laporte, L., Tortelli, F., Briquez, P. S. and Hubbell, J. A., "Engineering the Regenerative Microenvironment with Biomaterials," Adv. Healthc. Mater., 2(1), 57-71(2013).   DOI
12 Liu, W. F. and Chen, C. S., "Engineering Biomaterials to Control Cell Function," Materials Today, 8(12), 28-35(2005).   DOI
13 Tallquist, M. and Kazlauskas, A., "PDGF Signaling in Cells and Mice," Cytokine & Growth Factor Reviews, 15(4), 205-213(2014).   DOI
14 Qin, D., Xia, Y. and Whitesides, G. M., "Soft Lithography for Micro-and Nanoscale Patterning," Nature Protocols, 5(3), 491(2010).   DOI
15 Little, L., Healy, K. E. and Schaffer, D., "Engineering Biomaterials for Synthetic Neural Stem Cell Microenvironments," Chemical Reviews, 108(5), 1787-1796(2008).   DOI
16 Stroka, K. M., Gu, Z., Sun, S. X. and Konstantopoulos, K., "Bioengineering Paradigms for Cell Migration in Confined Microenvironments," Current Opinion in Cell Biology, 30, 41-50(2014).   DOI
17 McLennan, R., Dyson, L., Prather, K. W., Morrison, J. A., Baker, R. E., Maini, P. K. and Kulesa, P. M., "Multiscale Mechanisms of Cell Migration During Development: Theory and Experiment," Development, 139(16), 2935-2944(2012).   DOI
18 Polacheck, W. J., Zervantonakis, I. K. and Kamm, R. D., "Tumor Cell Migration in Complex Microenvironments," Cellular and Molecular Life Sciences, 70(8), 1335-1356(2013).   DOI
19 Clark, A. G. and Vignjevic, D. M., "Modes of Cancer Cell Invasion and the Role of the Microenvironment," Current Opinion in Cell Biology, 36, 13-22(2015).   DOI
20 Ghibaudo, M., Trichet, L., Le Digabel, J., Richert, A., Hersen, P. and Ladoux, B., "Substrate Topography Induces a Crossover from 2D to 3D Behavior in Fibroblast Migration," Biophysical Journal, 97(1), 357-368(2009).   DOI
21 Kim, D. H., Provenzano, P. P., Smith, C. L. and Levchenko, A., "Matrix Nanotopography as a Regulator of Cell Function," The Journal of Cell Biology, 197(3), 351-360(2012).   DOI
22 Shoichet, M. S., "Polymer Scaffolds for Biomaterials Applications," Macromolecules, 43(2), 581-591(2009).   DOI
23 Vozzi, G., Flaim, C., Ahluwalia, A., and Bhatia, S., "Fabrication of PLGA Scaffolds Using Soft Lithography and Microsyringe Deposition," Biomaterials, 24(14), 2533-2540(2013).   DOI