• 감성과학
• /
• 제14권4호
• /
• pp.531-536
• /
• 2011

감성과학에서 인간의 감성을 손쉽게 측정할 수 있는 기술이나 기법 등은 매우 중요한 분야이다. 최근 마음의 변화는 몸의 변화를 수반한다(정신신경증면역내분비학, psychoneuroimmunoendocrinology)는 전제하에 인간 감성을 체액을 통해서 측정하는 시도가 이루어지고 있다. 몸의 변화 중에 최근까지 가장 많이 연구된 것은 스트레스에 관한 것으로 이를 측정하는 지표로는 코티졸(cortisol)이라는 물질이 널리 알려져 오고 있다. 코티졸을 측정하는 기존의 방법을 열거하면 High Performance Liquid Chromatography (HPLC), fluorometric assay, reverse phase chromatography 등이 대표적인데 모두 시간이 많이 걸리고 가격이 비싸며 휴대용이 아니기에 POCT (point of care testing)에 적합하지 않다. 이에 본 연구진은 소형 초고주파 공진 소자를 만들어 타액 속의 코티졸을 측정할 수 있는 항체를 고정하고 이것이 코티졸과 결합할 때 나오는 공진신호를 읽음으로써 환자의 타액 속 코티졸을 쉽고 빠르게 측정할 수 있는 기법을 소개한다. 본 연구를 위해 제안된 공진소자는 밀리미터 크기의 소자로서 제작이 용이하며 간단한 형태이다. 최종적으로 소자표면상에 결합되는 코티졸 농도변화(100, 10, 1, 0.1 ng/ml)에 따라 거의 선형적인 주파수응답(11, 10, 9, 7 MHz) 특성을 보인다. 100 pg/ml에 해당하는 적은 코티졸의 양까지 거의 실시간에 가까운 빠른 시간 안에서 쉽게 검출이 가능하며, 뿐만 아니라 표지(labeling)가 필요 없는 장점을 가지고 있다. 이러한 농도에 따른 주파수 변화를 기반으로 하는 코티졸 감성지표센서는 무선단말시스템에 적용될 수 있는 가능성과 응용성을 가지고 있다.

• 대한임상검사과학회지
• /
• 제53권1호
• /
• pp.11-18
• /
• 2021

호흡기 바이러스는 감염된 방문자와의 직접적인 접촉을 통해 병원 환경에서 감염된다. 감염관리 분야에서 호흡기 바이러스에 의한 병원내 획득 감염의 주요한 문제를 유발한다. 임상 검사실에서 파생된 감시 데이터는 또한 의료 서비스를 제공하는 기관과 공중 보건 분야에서 치료, 소모품 및 진단 제품 구매를 위해 병원과 지역 사회에 의료자원을 적절하게 배분하기 위해 종종 사용된다. 호흡기바이러스의 감염에서 조기 진단은 필수적이며 호흡기 검체를 사용하는 진단법에 활용될 수 있는 방법에는 바이러스 배양, 분자 진단 및 분석 등이 포함된다. 랩온어칩(LoC)/마이크로칩은 보다 다양하고 강력한 기술인 차세대 현장검사 시험법을 개발할 수 있는 새로운 전략으로 제공한다. 호흡기계의 중요성은 의료관련 종사자의 직업적 건강과 안전을 보장하기 위해 감염 관리 지침에 엄격히 적용되어야 한다. 이 연구를 포함하여 점점 더 많은 임상적 효능 증거가 감염 전파에 대한 오랜 패러다임에 도전하고 있다. 바이러스의 감염 가능성을 의심하는 새로운 호흡기 증상이 시작된 입원 환자로부터 호흡기 바이러스를 탐지하기 위한 빈번한 검사에 대해 추가 지원이 요구되고 감염통제의 노력에 집중적으로 도움이 이루어져야 할 것이다.

• 지역사회간호학회지
• /
• 제10권1호
• /
• pp.53-69
• /
• 1999

A descriptive correlation study was done to provide a basic data for comprehensive nursing care by analyzing the relationship between spiritual wellbeing, hope and perceived health status of the elderly. 195 respondents who lived at their homes and 148 respondents who lived at the facilities for elders such as nursing homes and elder's rehabilitation centers were selected and their age was over 60 years old. Paloutzian and Ellison(1982)'s spiritual wellbeing scale, Nowotny(1989)'s hope scale and Northern Illinois University's health self rating scale was used. From August 10th to August 25th, 1998, ready made questionnaires were handed out by researcher to those who can fill it out and for those who cannot fill out the questionnaires alone, the researcher read it and finished by interview. This study used Pearson's correlation coefficient for the hypothetic test and the average point and standard deviation of spiritual wellbeing, hope, and perceived health status were checked. To find out the difference between spiritual wellbeing, hope, and perceived health status by general characteristics ANOVA and Tukey test were used. The results are as follows : 1. The mean score for spiritual wellbeing of the elders was 42.27($SD{\pm}9.67$) in a possible range of 20-80. The average point of spiritual wellbeing was 2.11($SD{\pm}0.97$) point to 4 point full marks. The mean score of religious wellbeing was 21.37($SD{\pm}7.02$) and that of existential wellbeing was 20.90($SD{\pm}4.63$) in a possible range of 10 - 40. The average point of religious wellbeing was 2.14($SD{\pm}0.70$)points and existential wellbeing was 2.09($SD{\pm}0.46$) points to 4 point full marks. 2. The mean score for hope was 67.16($SD{\pm}12.28$) in a possible range of 29-116. The average point of hope was 2.31($SD{\pm}0.42$) points to 4 point full marks. 3. The mean score for perceived health status was 8.72($SD{\pm}2.49$) in a possible range of 4-14. 4. In testing the hypothesis concerning the relationship between spiritual wellbeing and hope, there was a statistically positive correlation(r=0.5209, p=0.0001). 5. In testing the hypothesis concerning the relationship between spiritual wellbeing and perceived health status, there was a statistically positive correlation(r=0.1427, p=0.0081). 6. In testing the hypothesis concerning the relationship between hope and perceived health status, there was a statistically positive correlation(r=0.2797, p=0.0001). 7. There were significant differences in spiritual wellbeing according to sex, religion, and present occupation. 8. There were significant differences in hope according to residential places, age, religion, educational level, family status, average monthly pocket money. 9. There were significant differences in perceived health status according to residential places, sex, age, educational level, present occupation and family status. From the above results it can be concluded that: There was a positive correlation between spiritual wellbeing and hope, spiritual wellbeing and perceived health status, hope and perceived health status. When the nurse implicate the nursing intervention which can be promote the spiritual wellbeing and hope, elder's perceived health status also can be improved.

• 대한의생명과학회지
• /
• 제29권4호
• /
• pp.363-370
• /
• 2023

Human respiratory viral infections such as COVID-19 are highly contagious, so continuous management of airborne viruses is essential. In particular, indoor air monitoring is necessary because the risk of infection increases in poorly ventilated indoors. However, the current method of detecting airborne viruses requires a lot of time from sample collection to confirmation of results. In this study, we proposed a system that can monitor airborne viruses in real time to solve the deficiency of the present method. Air samples were collected in liquid form through a bio sampler, in which case the virus is present in low concentrations. To detect viruses from low-concentration samples, viral RNA was concentrated and extracted using silica-magnetic beads. RNA binds to silica under certain conditions, and by repeating this binding reaction, bulk samples collected from the air can be concentrated. After concentration and extraction, viral RNA is specifically detected through real-time qPCR (quantitative polymerase chain reaction). In addition, based on liquid handling technology, we have developed an automatic machine that automatically performs the entire testing process and can be easily used even by non-experts. To evaluate the system, we performed air sample collection and automated testing using bacteriophage MS2 as a model virus. As a result, the air-collected samples concentrated by 45 times then initial volume, and the detection sensitivity of PCR also confirmed a corresponding improvement.

• 비파괴검사학회지
• /
• 제35권2호
• /
• pp.128-133
• /
• 2015

원격진료를 요하는 응급현장에서 사용을 위하여 1차원 배열 탐촉자를 손으로 조작하는 동안 변하는 방사빔의 위치를 추적하여 정적인 3차원 초음파영상을 구성하였다. 탐촉자에 고정된 모션센서의 병진운동 및 회전운동에 대한 정보를 기준직교좌표계로 변환하는 이론적 모델을 제시하였으며, 신호 증폭과 시리얼 통신을 위한 인터페이스 용도의 모듈을 상용의 센서를 사용하여 제작하였다. 실리콘 퍼티로 제작된 시험용 팬텀에 대한 B-모드의 동영상과 센서의 신호를 동기화하여 기록하였다. 동영상으로부터 주기적으로 추출한 B-모드 영상과 각 영상내의 픽셀 각각의 그레이 레벨을 이용하여 3차원 복셀의 그레이 레벨을 재구성하였다. 이 복셀의 데이터를 이용하여 3차원 영상과 임의단면의 B-모드 방식의 2차원 영상을 구성하였으며, 이 영상들은 시험용 팬텀의 형상과 잘 일치하였다.

• Genomics & Informatics
• /
• 제21권2호
• /
• pp.24.1-24.7
• /
• 2023

Assays of clinical diagnosis and species identification using molecular markers are performed according to a quantitative method in consideration of sensitivity, cost, speed, convenience, and specificity. However, typical polymerase chain reaction (PCR) assay is difficult to quantify and have various limitations. In addition, to perform quantitative analysis with the quantitative real-time PCR (qRT-PCR) equipment, a standard curve or normalization using reference genes is essential. Within the last a decade, previous studies have reported that the digital PCR (dPCR) assay, a third-generation PCR, can be applied in various fields by overcoming the shortcomings of typical PCR and qRT-PCR assays. We selected Stilla Naica System (Stilla Technologies), Droplet Digital PCR Technology (Bio-Rad), and Lab on an Array Digital Real-Time PCR analyzer system (OPTOLANE) for comparative analysis among the various droplet digital PCR platforms currently in use commercially. Our previous study discovered a molecular marker that can distinguish Hanwoo species (Korean native cattle) using Hanwoo-specific genomic structural variation. Here, we report the pros and cons of the operation of each dPCR platform from various perspectives using this species identification marker. In conclusion, we hope that this study will help researchers to select suitable dPCR platforms according to their purpose and resources.

• 간호행정학회지
• /
• 제4권2호
• /
• pp.419-437
• /
• 1998

Nurses use a nursing process. that is a scientific approach method. in order to understand the clients' condition and to solve their problems. Professional nurses have accepted the nursing process as a standard framework for nursing activities, and the first step of the nursing process is nursing assessment. The purpose of this study was to develop a nursing assessment tool for emergency patients. Assessment is a first step in the nursing process, and scientific assessment helps to better understand the patients. Thus the development of an assessment tool for emergency patients will improve of nursing quality and advance to efficacy of emergency room(ER) management. The study involved the development of a conceptual framework, preliminary tool, content validity verification process. and reliability verification process. The conceptual framework was developed through a review of the literature. and preliminary tool was developed from the conceptual framework that based on care flow sheet' developed James A. Haley. The preliminary tool was evaluated for validity and reliability by seven experts, one ER nurse manager, two ER head nurses, two emergency medicine doctors. and two ER charge nurses and by ten nurses with one to five year careers in ER nursing. The results of this study was as follows 1. The conceptual framework which was developed was composed of three parts. They were triage criteria, first assessment and secondary assessment. 2. The preliminary tool which was developed had 31 items based on the conceptual framework. 3. To test expert validity a 4-point scale was used. items which had gained 3-4 points from six persons of the seven experts were selected. All 31 items from the preliminary tool were selected. 4. The reliability test was done by ten nurses educated in the use of the assessment tool. Two pairs of nurses simultaneously assessed the same patients. A total agreement percentile was calculated and result was 87.3% agreement. 5. After the validity and reliability testing. the final emergency patient nursing assessment tool was finalized with 31 items. and a check-list for the responses. This study concludes that the tool which was developed is both valid and reliable will advance quality care for emergency patients. This emergency nursing assessment tool was also found to be an adequate tool for assessment of emergency patients.

• 대한의용생체공학회:의공학회지
• /
• 제30권1호
• /
• pp.23-33
• /
• 2009

It can be used easily to reduce rehabilitation and treatment time if diagnostic and therapeutic devices are attached to cloth or body. Therefore we developed neuromuscular wearable ultra-miniature lighting modules which can improve the neuromuscular function and verified its clinical effectiveness. The system is based on the ultra-miniature lighting treatment module and there are two types of systems. One of them is designed as an attached type and the other type is combined with clothing. The wearable ultra-miniature lighting module is composed of controller (battery, MCU, bidirectional transmitter and receiver), cable, treatment medium generating device and other peripheral devices. To verify the clinical effectiveness of this device, we observed the difference of the strength of a muscle before and after 650nm and 25mW laser irradiation on the reflex point for 1 to 4 seconds. Among 48 patients having the degenerative osteoarthritis, the muscle strength before and after irradiation of laser was $21.8{\pm}7.99$ and $27.3{\pm}8.43$. According to the result, the muscle strength after treatment was significantly increased (p<0.01). To whom having difficulty in visiting to OPD(Out-Patient Department), doctors medically examine the patients and find the therapeutic point, attachment of this wearable ultra-miniature lighting module can function as self treatment (treating instrument) and treatment assist at home. If doctor can remotely control the patient and take part in treatment, the therapeutic device could contribute to prevention and care device.

• 한국의료질향상학회지
• /
• 제4권1호
• /
• pp.82-103
• /
• 1997

Background : With increased concerns about variation among physician's practice pattern and their impact on the quality of care, clinical practice guidelines have been developed by many different organizations, with differing aims and incentives. From the same point of view, there is growing interest in the development of clinical practice guidelines in Korea, but with only a few examples. As a result, there is not much exploration on the incentive and barrier to develop guidelines as well as description on the development process. The purposes of this study are to describe the process of the four different clinical practice guidelines in a hospital setting, and to identify incentives and barriers in the development of guidelines. Methods : For this research, a clinical practice guideline development committee and four clinical practice guideline development teams were organized in a university hospital which has more than 1,200 bead. Twenty eight doctors, three nurses, and one technician participated as members of development teams for eight months. Four to six meetings were held, and three to seven departments in the hospital were involved. Results : The topics which developed into clinical practice guidelines were cardiopulmonary resuscitation(CPR), blood transfusion, anticoagulation, and angiography. The main goals set by teams were education(CPR, angiography), risk management(blood transfusion), and to enhance quality of care(anticoagulation). Among four teams, only in the team for anticoagulation guideline medical record review and pilot-testing were performed. Also literature review was not carried out systematically. However, all the guidelines were developed by multidisciplinary be used as standard protocols in the practice. Conclusion : Experience and skill in developing process has to be improved to have a more valid and useful practice guideline. In particular, literature review and problem identification by examining medical record should be emphasized. Also further studies on the clinical outcomes of the guidelines application and changes in physicians' behaviors would be required.

• 한국보건간호학회지
• /
• 제18권2호
• /
• pp.342-357
• /
• 2004

A descriptive correlation study was done to provide a basic data for comprehensive nursing care by analyzing the relationship between spiritual wellbeing, hope and perceived health status of the rural elderly. 130 respondents who lived at their homes and nursing homes for elders in D county. Kyungbuk province were selected and their age was over 60 years old. Paloutzian and Ellison(1982)'s spiritual wellbeing scale. Nowotny(l989)'s hope scale and Northern Illinois University's health self rating scale was used. From September 2nd to September 30th, 2002, ready made questionnaires were handed out by researcher and two well trained nurse research assistants. to those who can fill it out and for those who cannot fill out the questionnaires alone, the researcher and assistants read it and finished by interview. This study used Pearson's correlation coefficient for the hypothetic test and the average point and standard deviation of spiritual wellbeing, hope, and perceived health status were checked. To find out the difference between spiritual wellbeing, hope, and perceived health status by general characteristics ANOVA and Tukey test were used. The results are as follows : 1. The mean score for spiritual wellbeing of the rural elders was $49.94(\pm5.62)$ in a possible range of 20-80. The mean score of religious wellbeing was $25.20(\pm3.91)$ and that of existential wellbeing was $24.74(\pm2.83)$ in a possible range of 10-40. The average point of religious wellbeing was $2.52(\pm0.39)$ points and existential wellbeing was $2.47(\pm0.28)$ points to 4 point full marks. 2. The mean score for hope was $67.68(\pm10.92)$ in a possible range of 29-116. The average point of hope was $2.33(\pm0.38)$ points to 4 point full marks. 3. The mean score for perceived health status was $9.95(\pm2.66)$ in a possible range of 4-14. The average point of perceived health status was $2.15(\pm0.72)$ point to 4 point full marks. 4. In testing the hypothesis concerning the relationship between spiritual wellbeing and hope, there was a statistically positive correlation(r=0.621. p=0.000). 5. In testing the hypothesis concerning the relationship between hope and perceived health status, there was a statistically positive correlation(r=0.305, p=0.000). 6. There were significant differences in spiritual wellbeing according to age(F=5.60, p=0.000), religion(F=11.61. p=0.000), family status(F=2.86, p=0.040) and average monthly pocket money(F=4.32, p=0.015). 7. There were significant differences in hope according to age(F=16.49, p=0.000), religion (F=3.56, p=0.009), educational level(F=8.94, p=0.000), present occupation(t=-3.13, p=0.002), family status(F=5.90, p=0.001) and average monthly pocket money(F=3.41. p=0.036). 8. There were significant differences in perceived health status according to present occupation(t=-2.16, p=0.033) average monthly pocket money(F=4.11, p=0.019). From the above results it can be concluded that: There was a positive correlation between spiritual wellbeing and hope, hope and perceived health status. There was no positive correlation between spiritual wellbeing and perceived health status. For futher study, adequate spiritual wellbeing scale and hope scale for rural elders should be developed and, age and religion factors has to be reconsidered. When the nurse implicate the nursing intervention which can be promote the spiritual wellbeing and hope, rural elder's spiritual wellbeing and hope can be improved and at the same time, their perceived health status also can be improved.