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Restriction endonuclease analysis of mitochondrial DNA of Acanthamoebn sp. YM-4 (Korean isolate) (Acanrhamoeba sp. YM-4의 미토콘드리아 DNA의 RFLP분석)

  • Sin, Ho-Jun;Im, Gyeong-Il;Jeon, Gwang-U
    • Parasites, Hosts and Diseases
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    • v.35 no.2
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    • pp.119-126
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    • 1997
  • Acanthnmoebn sp. YM-4 is simitar to A. culbertsoni based upon morphological characteristics of trophozoites and cysts. However, based on other characteristics, pathogenicity to mice, in uitro cytotoxicity and isoenzyme patterns, Acanthomoebo sp. YM- 4 was quite different from A. culbertsoni. Restriction fragment length polymorphism (RFLP) analysis of mtDNA is useful in the classification of members belonging to the genus Acanthcmoebn. Therefore, in this study, RFLP analysis of Acnnthcmoeba mtDNAs was accomplished using five restriction enzymes: Hnelll, Hinull, Clcl, Pudl and ScE. Each restriction enzyme produced approximately 3-15 fragments (range: from 0:6 kip to 34.4 kbp) . The mtDNA genome size, calculated by the summation of restriction fragments, averaged 46.4 kbp in Acnnthamoeba sp. YM-4,48.3 kbp in A. culbertsoni and 48.8 kbp in A. polyphaic, respectively. Digested mtDNA fragments of Accnthcmoeba sp. YM-4 contained nine and seven same size fragments, respectively, from a total of 67 and 69 fragments observed in A. culbertsoni and A. polyphcgn. An estimate of the genetic divergence was 10.1% between Acanthamoebc sp. YM-4 and A. culbertsoni, and 9.9% between Acanthamoebn sp. YM-4 and A. polyphcga.

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Accuracy Examination in the RCS Computation of a Leaf Using the Resistive Sheet Technique with Various Thicknesses and Moisture Contents (잎 두께와 수분함유량에 따른 손실판 방식 RCS 계산의 정확성 검증)

  • Park, Minseo;Kim, Han-Joong;Um, Kwiseob;Park, Sin-Myong;Kweon, Soon-Koo;Oh, Yisok
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.25 no.11
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    • pp.1183-1189
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    • 2014
  • The accuracy of the resistive-sheet technique in calculating the RCS(Radar Cross Section) of a deciduous leaf is examined in this paper for various thicknesses and dielectric constants, and a range of thicknesses for the resistive sheet technique is proposed. At first, a leaf was assumed to be a lossy dielectric disk, and the dielectric disk was again assumed to be a resistive sheet with an appropriate resistivity for a given thickness, a dielectric constant, and a frequency. Then, the RCS of the leaf was computed using the physical optics(PO) method, and was compared with the calculation results of a numerical analysis: i.e., a commercial tool based on the FEM (Finite Element Method) technique. It was shown that the error increases as the thickness increases. The error was 0.1 dB, for example, when the thickness is 1.2 mm and 3.7 dB when the thickness is 3 mm with a dielectric constant of(21.4, 9.7) at 9.6 GHz. It was also found that the error decreases as the dielectric constant increases. This study will be very useful for calculating the scattering characteristics of numerous leaves in a vegetation canopy for estimating its radar backscatter using scattering model.

Studies on the Structure of Plant Community and Visitor's Activities in Mt. Naejang Park (I) - Analysis of the Vegetational Structure by the Ordination Techniques - (내장산국립공원(內藏山國立公園)의 식물군집(植物群集) 및 이용행태(利用行態)에 관한 연구 (I) - Ordination 방법에 의한 식생구조분석(植生構造分析) -)

  • Lee, Kyong Jae;Oh, Koo Kyoon;Jo, Jae Chang
    • Journal of Korean Society of Forest Science
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    • v.77 no.2
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    • pp.166-177
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    • 1988
  • To investigate the structure of the plant community of the Naejang Temple District in Mt. Naejang National Park, 22 plots were set up by the vegetation physiognomy and vegetation analysis by four kinds of ordination techniques(PO, PCA, RA and DCA) was carried out. Pure and mixed forest community of Quercus variabilis and Q. mongolica were major forest communities and each of them covered 31.27 and 20.77%, respectively. The degree of human disturbance of vegetation 9, 8, 7 and 6 area covered 30.9, 67.0, 0 and 0.02%, respectively. According to stand dynamic analysis by DBH class distribution, the present Pinus densiflora and Quercus communities may be succeeded to Carpinus laxiflora communities. DCA was the most effective method of this study. DCA ordination showed that successional trends of tree species seem to be from P. densiflora through Q. variabilis, Q. aliena, Sorbus alnifolia, Platycarya strobilacea to Carpinus laxiflora, Fraxinus rhynchophylla, Zelkova serrata, Cornus controversa in the tree layer, and from Rhododendron mucronulatum, Rh. schlippenbachii, Lespedeza cyrtobotrya, Rhus trichocarpa through Styrax japonica, Daphniphyllum macropodum, Sapium japonicum to Euonymus oxyphyllum, E. sieboldiana, Lindera erythrocarpa in the subtree layer.

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Influence of Spa Sewage on the Water and Soil Pollution and Restoration I. Influence of Spa Sewage on the Pollution of Stream Water and Agricultural Land (온배수 유입 소형하천의 수질 및 토양오염과 회복에 관한 연구: I. 온배수가 인근 소하천과 농업 환경에 미치는 영향)

  • 정연태;이덕배;이경보;김미연;김백호;최민규;박승택
    • Korean Journal of Environmental Biology
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    • v.17 no.3
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    • pp.337-344
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    • 1999
  • This study was carried out to investigate the influence of spa sewage on water quality and chemical contents in the paddy soil along stream from 1997 to 1998. Concentration of $PO_4, SO_4, Cl, NH_{4}, Ca, Na$ and COD in the spa sewage were lower than standard for agricultural usage, and were lowered as the sewage flew to the into stream. The concentration of $SO_4$in spa sewage was over the criteria for agricultural usage in the inlet, but was lower than criteria for agricultural usage by inlet of non polluted stream water. Concentration of pollutants in the sediment of water channel were the highest in the inlet site. There were no pollutants accumulation in the paddy soil where spa sewage was irrigated. It may be resulted from nutrients uptake of rice plant and self purification of paddy soil. On the while, considering electric conductivity and nitrate in spa sewage, this results suggest that long-term irrigation of the spa sewage may be required general management with some decreasing fertilization.

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The Structure of Plant Community in Kwangnung Forest(I) -Analysis on the Forest Community of Soribong Area by the Classification and Ordination Techniques- (광릉(光陵) 삼림(森林)의 식물군집구조(植物群集構造)(I) -Classification 및 Ordination 방법에 의한 소리봉(蘇利峯)지역의 식생분석(植生分析)-)

  • Lee, Kyong Jae;Jo, Jae Chang;Lee, Bong Su;Lee, Do Suck
    • Journal of Korean Society of Forest Science
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    • v.79 no.2
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    • pp.173-186
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    • 1990
  • To investigate the structure of the plant community of Soribong area in Kwangnung forest, forty-six plots were set up by the clumped sampling method. The classification by TWINSPAN and four kinds of multivariate ordination(PO, PCA, RA, DCA) were applied to the study area in order to classify them into several groups based on woody plants and environmental variables. The classification had been successfully overlayed on an ordination of the same data using DCA. The plots can be classified into four groups by TWINSPAN and DCA. The successional trends of tree species by both techniques seem to be from Pinus densiflora through Quercus mongolica, Q. serrata, Q. aliena, Carpinus laxiflora, Sorbus alnifolia to C. cordata, Fraxinus rhynchophylla, Cornus controversa in the canopy layer, and from Rhododendron mucronulatum, Rhus triohocarpa, Lespeoleza cyrtobotrya, Weigela subsessilis through Corylus sieboldiana, Lindera obtusiloba to Slaphylea bumalda, Callicarpa japonica, Lonicera maackii in the understory layer. As a result of the analysis for the relationship between the stand scores of DCA and environmental variables, they had a tendancy to increase significantly from the P. densiflora community to C. cordata community that was soil pH and the amount of humus, total nitrogen and exchangeable cations.

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Optimal Culture Conditions for Masspropagation of Gametophytes and Sporophytes of Pyrrosia linearifolia by Tissue Culture (조직배양을 이용한 우단일엽의 대량번식을 위한 전엽체와 포자체의 적정 배양조건)

  • Shin, So Lim;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.3
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    • pp.179-185
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    • 2010
  • This study was carried out to investigate the optimal culture conditions for gametophytes growth and sporophytes regeneration of Pyrrosia linearifolia in order to provide for the masspropagation system foundation of Pyrrosia linearifolia using their life cycle. Among many different media, 2MS medium was most effective in prothallus proliferation. Prothallus growth was promoted as the total concentration of nitrogen sources increased, and the best result was observed on 120 mM nitrogen. The best concentration of sucrose was 3%. The addition of 5~20 mM IAA, NAA, BA and kinetin promoted the propagation of prothallus. But 2iP demonstrated the most inhibitory effect on prothallus proliferation. Gametophytes shaking-cultured with liquid medium showed similar growth with solid medium and normal formation of reproductive organs. Shoot regeneration was most effective on 1/8MS medium, but growth was promoted on 1/2MS medium. For promotion of shoot regeneration and growth, the suitable concentrations of sucrose and $NaH_2PO_4$ were 1% and $50{\sim}100mg{\cdot}mL^{-1}$ in 1/8MS medium, respectively.

A Study on the Spatial Composition and Area Distribution Functional shift in the Community Center - Focused on the Cases in Gunpo, Gyeonggi-do - (주민센터의 '기능전환'에 따른 공간구성과 면적배분에 관한 연구 - 경기도 군포시 사례를 중심으로 -)

  • Juen, Mu-Yeon;Park, Jae-Seung
    • Korean Institute of Interior Design Journal
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    • v.19 no.2
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    • pp.176-185
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    • 2010
  • Due to Korean government's reduction policy of the level of local administration, the government tries to become smaller and more efficient organization and in accordance with this movement, they try to transfer the human power and work of the office of Eup, Myeon, Dong to the office of Si, Goon, Goo so that they can take administrative action quickly, increase the efficiency of administration by preventing the overlapping of administrative task, improve the administrative service toward the citizens and make a functional shift of Inhabitant Self-Government Center in a way that is aimed at improving the quality of life of the inhabitants by setting up cultural, welfare facilities and forming the local community. Hereupon I expect this study will help to show the ways of composing the space of community center, accommodating the various demands of inhabitants through appropriate area calculation of functional area, increasing the efficiency of government officers' administrative support and also will be the guide for the architectural designs of the community centers. For this purpose, I have investigated community centers of 8 Dongs out of 11 Dongs in Goon-po Si that was chosen to be operating the pilot program of the office of Eup, Myeon, Dong's functional shift and obtained excellent results. I have surveyed how the functional spaces are being used, interviewed the government officials, examined the size of the functional and required space with Building card of a plan and the analyzed results are as follows.

A Design of Predistortion HPA using Frequency Up-Conversion Mixing Operation (주파수 상향 변환을 이용한 전치왜곡 전력 증폭기 설계)

  • Jeong, Yong-Chae;Kim, Young;Kim, Chul-Dong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.15 no.5
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    • pp.480-485
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    • 2004
  • In this paper, a new predistortion linearizing method using frequency up-conversion operation of mixer is proposed. This linearizing method doesn't require any additional signal sources in spite of frequency up-conversion mixing operation. This method extracts the 2nd low frequency intermodulation distortion signal from input signals and uses the extracted 2nd low frequency intermodulation distortion signal as mixing signal source. To show validation of the proposed predistortion method, we made K-PCS power amplifier. On 2-tone signals amplification process, the (C/I) ratio of amplifier is improved 26 ㏈ (@Po=22 ㏈m/tone), where two tones are 1.8544 ㎓ and 1.8556 ㎓, respectively. And (C/I) ratio is improved more than to 20 ㏈ for 17 ㏈ output signal dynamic range. On IS-95A CDMA 1FA amplification process, the improvements of adjacent channel power ratio(ACPR) are 10.8 ㏈ and 6.4 ㏈ at ${\pm}$885 ㎑ and ${\pm}$1.25 ㎒ offset points, respectively.

Analysis of Relationship between Mixed Venous PO2 and Status of Cardiac Performance with Hemodynamic Values after Correction of Cyanotic Congenital Heart Disease (청색심기형 교정술후 혼합정맥혈 산소분압과 심근상태 및 혈류역학치와의 상관관계 분석)

  • An, Jae-Ho;Kim, Yong-Jin
    • Journal of Chest Surgery
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    • v.22 no.2
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    • pp.212-219
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    • 1989
  • We utilized pulmonary artery pressure monitoring system in risky patients for preventing the postoperative pulmonary hypertensive crisis and for sampling the mixed venous blood. And this mixed venous blood oxygen saturation [MVSO2] or partial pressure [MVPO2]tells us many meaningful patients state. We selected 59 cyanotic congenital heart diseased patients, who were operated in our hospital from Nov. 1987 to Oct. 1988, in the Department of Thoracic and Cardiovascular Surgery, Seoul National University Children\ulcorner Hospital, who had pulmonary artery pressure monitoring catheter and who made us know their mixed venous oxygen condition. We found that there was no close relationship between MVPO2 and Cardiac Index [C.I.] during early postoperative period, but on the first and second day after operation the correlation coefficient was increased as r=0.35[p=0.008], r=0.78[p=0.0001]. So we concluded that the correlation between MVPO2 and C.I. was more reliable with time going as hemodynamic stabilization. And we experienced no survivors whose MVPO2 was under 20 torr, but that was not the only factor for death. From these results, we conclude that we can consider the MVPO2 [or MVSO2] representing C.I. after stabilized postoperative condition of the open heart surgery patients, but during early postoperative period, in addition to this MVPO2, we should do also apply other parameter such as urine output, arterial blood pressure, left atrial pressure and pulmonary arterial pressure for exact estimation of the patients status.

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Synthesis and Biological Activities of Some New 3,6-Disubstituted 1,2,4-Triazolo[3,4-b]1,3,4-thiadiazole Derivatives

  • Rafiq, Muhammad;Saleem, Muhammad;Hanif, Muhammad;Maqsood, Muhammad Rizwan;Rama, Nasim Hasan;Lee, Ki-Hwan;Seo, Sung-Yum
    • Bulletin of the Korean Chemical Society
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    • v.33 no.12
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    • pp.3943-3949
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    • 2012
  • A series of aromatic hydrazides 3a-j were prepared by refluxing esters 2a-j with hydrazine hydrate in methanol, which were prepared by the esterification of 1a-j. Acetohydrazides 3a-j upon treatment with carbon disulfide and methanolic potassium hydroxide yielded potassium dithiocarbazate salts 4a-j, which on refluxing with hydrazine hydrate yielded substituted 4-amino-5-aryl-3H-1,2,4-triazole-3-thiones 5a-j. The target compounds 6a-j were synthesized by condensing furan-3-carboxylic acid in the presence of polyphosphoric acid under reflux. The structures of newly synthesized compounds were characterized by IR, $^1H$ NMR, $^{13}C$ NMR, elemental analysis and mass spectrometric studies. All the synthesized compounds were screened for their urease, acetylcholine esterase inhibition, antioxidant and alkaline phosphatase inhibition activity. Almost all of the compounds 6a-j showed good to excellent activities against urease and acetylcholine esterase more than the reference drugs. Compounds 6f and 6g were more potent scavenger of free radicals than the reference n-propyl gallate. Compound 6b and 6h showed excellent activities of alkaline phosphatase as compare to the reference $KH_2PO_4$.