• Title/Summary/Keyword: PoF

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19F NMR Investigation of F1-ATPase of Escherichia coli Using Fluorinated Ligands

  • Jung, Seun-Ho;Kim, Hyun-Won
    • BMB Reports
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    • v.31 no.1
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    • pp.44-47
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    • 1998
  • Asymmetry amongst nucleotide binding sites of Escherichia coli $F_1$-ATPase was examined using $^{19}F$ NMR signal from fluorinated analogs of adenine nucleotides bound to nucleotide binding sites. ADP-$CF_2-{PO_3}^{2-}$ showed no inhibitory effect to $F_1$-ATPase. But ADP-CHF-${PO_3}^{2-}$ (racemic mixture) showed competitive inhibition of $F_1$-ATPase with $K_i$ of $60\;{\mu}m$. ADP-CHF-${PO_3}^{2-}$ shows only negligible binding to $EF_1$ in the absence of $Mg^2+$. With the addition of $Mg^2+$ to the medium, the $^{19}F$ resonance of free ADP-CHF-${PO_3}^{2-}$ disappeared and the new broad resonances appeared. Appearance of more than two new asymmetric resonances following the binding of ADP-CHF-${PO_3}^{2-}$ to $EF_1$ may indicate that at least one of the isomers showed split resonances. This may suggest that the region between ${\alpha}$-and ${\beta}$-phosphate of ADP-CHF-${PO_3}^{2-}$ which is bound to catalytic sites is experiencing a different environment at different sites.

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A Study on the Modeling of PoF Estimation for Probabilistic Risk Assessment based on Bayesian Method (확률론적 위험도평가를 위한 베이지안 기반의 파손확률 추정 모델링 연구)

  • Kim, Keun Won;Shin, Dae Han;Choi, Joo-Ho;Shin, KiSu
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.41 no.8
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    • pp.619-624
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    • 2013
  • To predict the probabilistic service life, statistical factors should be included to consider the uncertainty of parameters. Generally the probabilistic analysis is one of the common methods to account the uncertainty of parameters on the structural failure. In order to apply probabilistic analysis on the deterministic life analysis, it would be necessary to introduce Probability of Failure(PoF) and conduct risk assessment. In this work, we have studied probabilistic risk assessment of aircraft structures by using PoF approach. To achieve this goal, the Bayesian method was utilized to model PoF estimation since this method is known as the proper method to express the uncertainty of parameters. A series of proof tests were also conducted in order to verify the result of PoF estimation. The results from this efforts showed that the PoF estimation model can calculate quantitatively the value of PoF. Furthermore effectiveness of risk assessment approach for the aircraft structures was also demonstrated.

Optical spectroscopy of Tb3+ ions doped NaCa(PO3)3 phosphors (Tb3+ 이온이 첨가된 NaCa(PO3)3 형광체의 형광특성)

  • Yoon, Changyong
    • Journal of the Korean Society of Radiology
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    • v.12 no.4
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    • pp.451-457
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    • 2018
  • Luminescence properties of $NaCa(PO_3)_3$ doped with $Tb^{3+}$ ions are investigated by optical and laser excitation spectroscopy. The phosphors were prepared by solidstate reaction method The X-ray diffraction(XRD) was used to analyze the crystal structure and the crystallinity of the samples. The excitation and emission spectra and decay curve of $NaCa(PO_3)_3:Tb^{3+}$(0.01 ~ 30mol%) were measured at room temperature. The f - d band of $Tb^{3+}$ is observed in the excitation spectra of $NaCa(PO_3)_3:Tb^{3+}$ in the wave length region 205 ~ 245 nm. Strong emission lines due to the $^5D_4{\rightarrow}^7F_J$ transition and weak emission lines due to the $^5D_4{\rightarrow}^7F_J$ transition are observed in the emission spectra of $NaCa(PO_3)_3:Tb^{3+}$. The energy transfer and cross relaxation between $Tb^{3+}$ ions are discussed in $NaCa(PO_3)_3:Tb^{3+}$ in the emission spectra and life time.

A Row Decoder Design and Simulation Considering The Characteristics of PoRAM (PoRAM의 특성을 고려한 행 디코더 설계 및 시뮬레이션)

  • Park, Yu-Jin;Kim, Jung-Ha;Cho, Ja-Young;Lee, Sang-Sun
    • Proceedings of the IEEK Conference
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    • 2006.06a
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    • pp.659-660
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    • 2006
  • The low crosstalk row-decoder is studied for PoRAM applications. Because polymer-based memories can be more densely integrated than established silicon-based ones, PoRAM is highly sensitive for the crosstalk problem. To overcome the problem and to suggest the suitable decoder for PoRAM, this paper shows the comparison of the row-path characteristics for both the 2-stage dynamic logic decoder and the 2-stage static logic decoder. Moreover, to suppress the Glitch effect which is observed by using the static logic decoder, the Master-Slave(M/S) D-Flip/Flop(D-F/F) is applied as a deglitch. Finally, the improved output result of the 2-stage static logic decoder with the M/S D-F/F is shown..

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AN EXPERIMENTAL STUDY OF THE EFFECT OF FLUORIDES ON HUMAN DENTIN (불화물(弗化物)의 상아질도포효과(象牙質塗布效果)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Chun, Dong-Moon;Min, Byung-Soon;Choi, Ho-Young;Park, Sang-Jin
    • Restorative Dentistry and Endodontics
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    • v.13 no.1
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    • pp.23-38
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    • 1988
  • In order to investigate the material formed by application of fluoride on human dentin, exposed dentin of tooth cervix and sound dentin powder was treated by several fluoride solutions. The former was observed by scanning electron microscope and the latter was analysed by x-ray diffractometer and infrared spectrophotometer. The results obtained were as follows: 1. Application with NaF, $SnF_2$ and $Na_2PO_3F$ on exposed dentin of tooth cervix formed mineralized materials covered. 2. The amount of mineralized materials increased with time intervals of applying the fluorides. 3. The density of mineralized materials was highest in $Na_2PO_3F$ treatment group, and NaF treatment group was higher than $SnF_2$ treatment group. 4. $SnF_2$ remained in covering of mineralized materials. 5. Application with NaF and $Na_2PO_3F$ formed mineralized materials characterized apatitic crystals. 6. The crystallinity of mineralized materials was higher prominently in $Na_2PO_3F$ treatment group.

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Development of Reliability Simulator for Electronic Components (전자부품 통합 신뢰성 Simulator 개발)

  • Kim, Wan-Doo;Lee, Seung-Woo;Han, Seung-Woo;Osterman, Michael
    • Proceedings of the KSME Conference
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    • 2007.05a
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    • pp.1749-1753
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    • 2007
  • The reliability, that is Long-Term Quality, require an approaching different from Short-Term Quality which is used before. As the electronic components are able to be easily normalized on the reliability testing, various testing standards are used. In this study, we proposed two reliability simulator that is PoF(Physics of Failure)-based and failure rate models-based. PoF-based simulator is introduced based on CalceEP program that is created by University of Maryland. This simulator can be modified by user interface of properties and PoF models and operated on stand alone system. Failure rate models-based simulator introduced according to analyzing reliability prediction documents. Also, unified database including failure data models is built from existing MIL-HDBK-217F N2, PRISM, and Bellcore, and web-based simulator is developed. The developed reliability simulator will service of the PoF model, properties, failure rate model accumulated and its data by web and internet.

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The Effect of Electroacupuncture at the ST36 on the Electroencephalogram (족삼리(ST36) 전침 자극이 뇌파에 미치는 영향)

  • Gwon, Sun-Cheol;Youn, Dae-Sik;Lee, Sang-Ryong
    • Korean Journal of Acupuncture
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    • v.23 no.1
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    • pp.15-36
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    • 2006
  • Objectives . The aim of this study was to examine the effect of electroacupuncture(EA) at the ST36 on normal humans by using power spectral analysis. Methods : EEG(Electroencephalogram) power spectral exhibits site-specific and state-related differences in specific frequency bands. In this study, power spectrum was used as a measure of complexity. 32 channel EEG study was carried out in 12 subjects (10 males; age=26.7 years old, 2females; age=28 years old). Results ; In ${\alpha}$ (alpha) band, the power values at Fp2, F7, F3, Fz, FTC1, FTC2, T3, C3, Cz, C4, TT1, TCP1, CP1, CP2,T5, P3, Pz, P4, Po1, Po2, O1, Oz,O2 channels(p<0.05) during the ST36-acupoint treatment were significantly increased. In ${\beta}$ (beta) band, the power values at Fp2, F7, F3, Fz, F4, F8, FTC1, FTC2, T3, C3, Cz, C4, TT1, TCP1, CP1, CP2, T5, P3, Pz, P4, Po1, Po2, O1, Oz, O2 channels(p<0.05) during the ST36-acupoint treatment were significantly decreased. In ${\delta}$ (delta) band, the power values at F7, Fz, T3, C3, TT1, TCP1, CP1, CP2, T5, P3, Pz,T6, Po1, PO2,O1, Oz, O2 channels(p<0.05) during the ST36-acupoint treatment were significantly decreased. In ${\theta}$(theta) band, the power values at F7, Fz, FTC1, T3, TCP1, CP2, TCP2, Po1, Po2 channels(p<0.05) during the ST36-acupoint treatment were significantly decreased. ${\alpha}$/${\beta}$ values at Cz, T5, O1, Oz, O2 channels during the ST36-acupoint treatment were increased. ${\beta}$/${\theta}$ values at Fpl, F7, F3, Fz, F4, F8, FTC1, FTC2, T3, C3, C4, T4, TT1, TCP1, TCP2, TT2, P3, P4, T6, Pol channels during the ST36-acupoint treatment were increased. Conclusions : This results suggest that Electroacupuncture at the ST36 mostly affects the charge on alpha(23 channels), beta(25 channels) bands.

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Electrochemical Characteristics of Nano-sized A2MnPO4F (A = Li, Na) as Cathode Materials for Lithium ion Batteries

  • Cho, Woosuk;Song, Jun Ho;Kim, Sang-Min;Kim, Dong-Jin;Kang, Min-Gu;Kim, Jeom-Soo;Kim, Young-Jun
    • Journal of Electrochemical Science and Technology
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    • v.4 no.3
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    • pp.113-118
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    • 2013
  • Fluorophosphate, $Na_2MnPO_4F$ as new cathode material was synthesized by carbothermal treatment method. Prepared $Na_2MnPO_4F$ has particle size under 100 nm and residual carbon exists in surface of $Na_2MnPO_4F$. Additional carbon coating was performed in order to increase the electrochemical properties. Even capacity and overpotential were improved by carbon coating using mechanical ball milling, the reduced crystallinity limited the drastic improvement of the electrochemical properties. To solve this problem, re-heat treatment was involved to recover crystallinity and then notable improvement of electrochemical properties was obtained. Specific amount of $Li^+$ that participates in electrochemical $Li^+$ insertion / extraction reaction, was x = 1 in $Li_xNa_{2-x}MnPO_4F$ within the voltage range of 2.0 to 4.8 V. The doubled capacity by 2 electron reaction can be obtained when NMPF is charged to higher voltage over 4.8 V.

Solubilization of Hardly Soluble Phosphates and Growth Promotion of Maize (Zea mays L.) by Penicillium oxalicum Isolated from Rhizosphere

  • SHIN WANSIK;RYU JEOUNGHYUN;CHOI SEUNGJU;KIM CHUNGWOO;GADAGI RAVI;MADHAIYAN MUNUSAMY;SESHADRI SUNDARAM;CHUNG JONGBAE;SA TONGMIN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.6
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    • pp.1273-1279
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    • 2005
  • Penicillium oxalicum strain CBPS-3F-Tsa, an efficient phosphate solubilizing fungus, was evaluated for its production of organic acid in vitro and effect of inoculation on the growth promotion of Maize under greenhouse conditions. The fungus solubilized 129.1, 118.8, and 54.1 mg P/1 of tricalcium phosphate [$Ca_{3}(PO_{4})_{2}$], aluminum phosphate ($A1PO_{4}$),and ferric phosphate ($FePO_{4}$), respectively, after 72 h of incubation. Malic acid, gluconic acid, and oxalic acid were detected in the flasks supplemented with various phosphate sources [240, 146, 145 mM/1 $A1PO_{4},\;FePO_{4},\;and\;Ca_{3}(PO_{4})_{2}$, respectively] together with a large amount of malic acid followed by the other two. The effects of inoculation of P. oxalicum CBPS-3F-Tsa on maize plants were studied under pot culture conditions. P. oxalicum CBPS-3F-Tsa was inoculated to maize plants alone or together with inorganic phosphates in the form of fused phosphates (FP) and rock phosphates (RP). Inoculation of P. oxalicum CBPS-3F-Tsa increased the plant growth and N and P accumulation in plants, compared with control plants, and also had positive effects when applied with RP. The results of this study show that the fungus P. oxalicum strain CBPS-3F-Tsa could solubilize different insoluble phosphates by producing organic acids, particularly malic acid, and also improved the efficiency of RP applied to maize plants.