• Title/Summary/Keyword: Pleurotus ostreatus

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Phylogenetic relationships of Pleurotus species based on RAPD analysis

  • Choi, Sun-Gyu;Jang, Kab-Yeul;Kim, Gyu-Hyun;Kong, Won-Sik;Jo, Jae-Sun;Kim, Hae-Yeong;Yoo, Young-Bok
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.154-162
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    • 2014

  • Oyster mushrooms including of P. ostreatus, P. eryngii, P. pulmonarius and P. cornucopiae are one of the famous mushrooms for foods in Korea. RAPD were carried out using 14 of oligoprimers to analyze the phylogenetic relationship among 57 strains of 32 Pleurotus species. Most of species formed the minimum clade with strains within species and was divided respectively species. Therefore clade was separated well in accordance species. Pleurotus species formed again clade to be added in close related to other species, and were discriminated by sixteen clades with each representative species including high similarity groups. Sixteen clades were composed representative species according to each clade. There were clade I of P. pulmonarius(P. sajor-caju, P. opuntiae, P. sapidus), clade II of P. eryngii(P. fuscus var. ferulae, P. fossulatus), clade III of P. ostreatus(P. ostreatus var. columbinus, P. spodoleucus, P. floridanus), clade IV of P. florida, clade V of P. djamor(P. flabellatus, P. incarnates, P. salmoneo-stramineus), clade VII of P. populinus(P. subareolatus), clade VIII of P. cystidiosus(P. cystidiosus var. formosensis), clade X of P. dryinus(P. dryinus var. pometi), clade XIV of P. cornucopiae(P. citrinopilieatus, P. euosmus), and clade XV of P. australis. These species were representative species each clades. Five species, P. ulmarius(clade VI), P. griseus(clade IX), P. calyptratus(clade XI), P. lampas(clade XII), P. smithii(clade XIII)and P. serotinus (clade XVI) were used each one strain in analysis, so they were clustered other groups.

  • https://doi.org/10.14480/JM.2014.12.3.154 인용 PDF KSCI

The Effects of the Quantities of the Rice Straw Substrates and Spawn on the Yield of Oyster Mushroom Pleurotus ostreatus (느타리버섯(Pleurotus ostreatus) 재배(裁培)에 있어서 배지량(培地量) 및 종균(種菌) 재식량(載植量)이 자실체(子實體) 수량(收量)에 미치는 영향(影響))

  • Park, Yong-Hwan;Chang, Hak-Gil;Ko, Seung-Joo
    • The Korean Journal of Mycology
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    • v.5 no.1
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    • pp.1-5
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    • 1977

  • The studies were carried out to examine the influence of the quantities of the rice straw substrate and spawn on the yield in the cultivation of oyster mushroom, Pleurotus ostreatus(Fr.) Quel. using rice straw as growing substrate. The best yield of fresh sporophres was 102 kgs. when the substrate was increased by up to 90 kgs. per 3.3 sq. metre. In case of distributing the spawn over the surface, it was regularly possible to increase the yield using spawn rate of 8kgs. per 3.3 sq. metre and by increasing the ratio of spawn in the substrate, the mycelial growth also made rapid progress.

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Transfer of Isolated Nuclei from Pleurotus florida into Protoplasts of Pleurotus ostreatus (느타리버섯 원형질체내(原形質體內)에 사철느타리버섯 핵(核)의 전이(轉移))

  • Yoo, Young-Bok;You, Chang-Hyun;Shin, Pyung-Gyun;Park, Yong-Hwan;Chang, Kwon-Yawl
    • The Korean Journal of Mycology
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    • v.15 no.4
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    • pp.250-253
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    • 1987

  • The transfer of the isolated nuclei from P. florida into protoplasts of P. ostreatus was induced with polyethylene glycol and $CaCl_2$. Three types of transfer products of nuclei were obtained when transferred to MMM. Type 1 colonies were more vigrously growing mycelium and stable on MCM. One of the type 1 colonies, appeared segregation on MCM plus benomyl. The mycelium did not form clamp connection. These results suggest that type 1 colonies were nuclear hybrids or allodiploids. Type 2 was main products of nuclear transfer. The mycelium formed clamp connection and fertile on sawdust media. Type 3 was very slow growing or non-viable colonies after debris of nuclei or chromosomes transfer into recipient protoplasts. Isozyme pattern of esterase in type 1 produced a new band. Type 2 and type 3 could be characterized by parental bands.

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