• 대한방사선기술학회지:방사선기술과학
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• 제21권1호
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• pp.79-87
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• 1998

이 실험의 목적은 4.5 및 7.5 Gy의 단일 선량으로 감마선의 전신조사에 따른 혈장 및 좌골신경 혈소판-유래성 성장인자(platelet-derived growth factor, PDGF)의 농도와 좌골신경 PDGF ${\alpha}$ -및 ${\beta}$ -수용체 밀도에 대한 시간의존성 변화를 조사 추구하는데 있다. 웅성 흰쥐(Sprague-Dawley계, 체중 $270{\pm}15.4g$)를 사용하여 $^{60}Co$ 감마선을 전신 조사(선량률 1.065 Gy/min)하고, 조사 후 1, 2, 3, 5 및 10일구에서 피폭에 따른 급성반응을 조사 추구하였다. 실험의 결과는 다음과 같다. 1) 4.5 및 7.5 Gy 선량군에서는 혈장 PDGF의 농도가 전 일구에 걸쳐 대조군($2.26{\pm}0.31\;ng/ml$)에 비해서 감소경향이 나타났으며, 한편 4.5 및 7.5 Gy 선량군 사이에서는 전 일구에 걸쳐 혈장 PDGF의 농도차에 유의적인 차이가 없었다(P>0.05). 피폭된 흰쥐의 혈장 PDGF의 농도는 4.5 및 7.5Gy 선량군에서 모두 대조군의 흰쥐에 비하여 5일구($4.5Gy:1.35{\pm}0.23\;ng/ml$; 대조군의 백분율로서 59.7%, 그리고 $7.5\;Gy:1.24{\pm}0.13\;ng/ml;54.9%$) 및 10일구($4.5Gy:1.27{\pm}0.28ng/ml;56.2%$ 그리고 $7.5Gy:1.07{\pm}0.19\;ng/ml:47.3%$)에서 각각 유의성으로 낮았다(P<0.05). 2) 4.5 및 7.5Gy 선량군에서는 좌골신경 PDGF의 농도가 전 일구간에 걸쳐 대조군($3.68{\pm}0.12\;ng/ml$)에 대하여 대체로 약간의 증가가 나타났으나, 한편 4.5 및 7.5Gy 선량군 사이에서 전 일구에 걸쳐 좌골신경 PDGF의 농도차는 무의성이었다(P>0.05). 피폭된 흰쥐의 좌골신경 PDGF의 농도는 4.5 및 7.5Gy 선량군에서 모두 대조군에 비하여 1일구($4.5Gy:4.33{\pm}0.03\;ng/m1$;대조군의 백분율로서 118% 그리고 $7.5Gy:4.81{\pm}0.36\;ng/ml$; 130%)에서 각각 증가하였으나 무의성이었다(P>0.05). 3) 방사선의 조사는 혈장 및 좌골신경 PDGF의 농도의 변화를 초래할 뿐만아니라 좌골신경 PDGF ${\alpha}$ - 및 ${\beta}$ -수용체 밀도의 변화도 초래하였다. 수용체의 밀도단위를 단백질의 상대적 함량(relative level of protein)으로 표시하고 일구-필적의 대조군을 백분율로 표시하였다. 4.5 및 7.5Gy 선량군에서의 좌골신경 PDGF ${\alpha}$ -수용체의 밀도는 대조군에 비하여 초기에 감소현상으로 나타나, 1일구에서는 40%(4.5Gy)와 50%(7.5Gy)를, 그리고 2일구에서는 33%(4.5Gy)와 50%(7.5Gy)로 가장 낮은 값을 나타낸 후 점차 대조군에 근접하는 값으로 증가하는 경향이었으며, 한편 4.5 및 7.5Gy 선량군에서 좌골신경 PDGF ${\beta}$ -수용체의 밀도는 역시 대조군에 비하여 각각 26%(4.5 Gy)와 27%(7.5Gy)의 최소 값을 나타낸 후점차 대조군에 근접하는 값으로 증가하는 경향으로 나타났다. 이러한 실험의 결과를 미루어 보아서, 혈장 및 좌골신경의 PDGF의 농도와 좌골신경 PDGF ${\alpha}$ - 및 ${\beta}$ -수용체의 밀도에 대한 방사선-유발성 변경이 골수 간세포 및 말초뉴런의 장해의 병인론에 관련되어 있는 것으로 생각된다.

• 한국동물학회지
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• 제33권3호
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• pp.266-275
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• 1990

Onocogene의 일종인 c-raf protein kinase는 세포질 속에 존재하는 serine / threonine-speccific protein이며, 이것은 mitogene signal에 의해 활성화된다. c-raf protein kinase의 구조와 기능은 protein kinase C와 매우 유사한 것으로 생각된다. 면역세포화학적으로 c-raf protein kinase의 signal transduction을 조사하기 위하여 EC-4 세포에 tumor promotor인 12-0-tet-radecanoylphorbol-13-acetae와 mitogenic gactor인 platelet-derived growth factor로 time-course에 따라서 처리하였다. Translocotion되는 c-raf는 먼저 perinuclear membrane에 모이고 그 후에 핵내로 이동되었다. 그런데 TPA와 PDGF로 처리한 c-raf의 translocotion은 각각의 다른 경로를 가짐을 알 수 있었다. TPA와 PAGF을 장기간 처리하였을 때, c-raf protein kinase의 down regulation이 유도됨을 알 수 있었다.

• Journal of Periodontal and Implant Science
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• 제26권1호
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• pp.80-88
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• 1996

The aim of the present investigation was to see the effect of combined use of PDGF BB and IGF -1 on the guided tissue regeneration(GTR) using barrier membrane in the treatment of human furcation involvement. Twelve patients with initially diagnosed as having moderate to advanced adult periodontitis with mandibular class II buccal furcation defects have been wer selected. Initial scaling and root planing has been performed and baseline data consisting of probing depths and attachment levels have been recorded prior to surgical procedures. The GTR procedures using either barrier membrane(control : ePTFE) alone or together with the application of PDGF - BB and IGF -l(experimental : ePTFE+PDGF/IGF) have been done under the routine guidelines. During the surgery, the distance from CEJ either to the bottom of the bone defects(CEJ - BD) or to the bone crest(CEJ-BC) were measured. Horizontal distance to the deepest area in the furcal defects were measured from the reference line connection the most prominent bony walls of the two buccal roots. 6 months following the GTR therapy, all the measurements were made repeatedly. The probing attachment gain of the experimental and the control grous were 2.14mm and l.07mm, respectively with no statically significnant difference. Amont of vertical bone fill in the experimental and the control groups were 2.43mm and 2.29mm, rexpectively. Amonut of horizontal bone fill were 2.86mm in the experimental group and 2.17mm in the control group, respectively. However, there were no significant differences in the amount of bone fill(both vertical and horizontal)between the two groups.

• The Korean Journal of Physiology and Pharmacology
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• 제23권2호
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• pp.141-150
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• 2019

Despite increased evidence of bio-activity following far-infrared (FIR) radiation, susceptibility of cell signaling to FIR radiation-induced homeostasis is poorly understood. To observe the effects of FIR radiation, FIR-radiated materials-coated fabric was put on experimental rats or applied to L6 cells, and microarray analysis, quantitative real-time polymerase chain reaction, and wound healing assays were performed. Microarray analysis revealed that messenger RNA expressions of rat muscle were stimulated by FIR radiation in a dose-dependent manner in amount of 10% and 30% materials-coated. In 30% group, 1,473 differentially expressed genes were identified (fold change [FC] > 1.5), and 218 genes were significantly regulated (FC > 1.5 and p < 0.05). Microarray analysis showed that extracellular matrix (ECM)-receptor interaction, focal adhesion, and cell migration-related pathways were significantly stimulated in rat muscle. ECM and platelet-derived growth factor (PDGF)-mediated cell migration-related genes were increased. And, results showed that the relative gene expression of actin beta was increased. FIR radiation also stimulated actin subunit and actin-related genes. We observed that wound healing was certainly promoted by FIR radiation over 48 h in L6 cells. Therefore, we suggest that FIR radiation can penetrate the body and stimulate PDGF-mediated cell migration through ECM-integrin signaling in rats.

• Journal of Periodontal and Implant Science
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• 제30권1호
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• pp.27-39
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• 2000

To evaluate the effects of Dexamethasone(Dex), Platelet derived growth factor-BB(PDGF) and combination of Dex and PDGF(DP) on the growth and differentiation of MC3T3-E1 cells, Dex($10^{-7}\;M$) and PDGF(10 ng/ml) in experimental group were added to the cells at the days 5, 10, 15, 20, 25 and examined for cell proliferation activities, DNA synthesis activities, ALP activities and bone nodule formation. The results were as follows : 1. In Dex group, cell proliferation, DNA synthesis and ALP activities were lower until 15 days when compared to the control group. Bone nodules formation were shown at 10 days. 2. In PDGF group, cell proliferation and DNA synthesis activities were higher until 15 days and ALP activities were lower when compared to the control and Dex groups. Bone nodules formation were shown at 20 days. 3. In DP group, cell proliferation and DNA synthesis activities of PDGF were suppressed by Dex and synergistic effects of combination of Dex and PDGF on ALP activities were shown at days 5 when compared to control and Dex groups. Bone nodules formation activities of Dex were suppressed by PDGF.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.84-84
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• 2002

This study was to investigate generation of the specific neuronal cell in vitro from the neural progenitors derived from human embryonic stem (hES, MB03) cells. For the neural progenitor cell formation, we produced embryoid bodies (EB: for 5 days, without mitogen) from hES cells and then neurospheres (for 7-10 days, 20 ng/㎖ of bFGF added N2 medium) from EB. And then for the differentiation into neuronal cells, neural progenitor cells were cultured in N2 medium (without bFGF) supplemented with brain derived neurotrophic factor (BDNF, 5 ng/㎖) or platelet derived growth factor-bb (pDGF-bb, 20ng/㎖) for 2 weeks. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.108.2-108.2
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• 2003

Kaempferol, a flavonol compound, has been reported as the anti-oxidant and anti-angiogenic agent and it has been found to inhibit cell growth in vitro. Abnormal proliferation of vascular smooth muscle cells (VSMCs) plays an important role in development of atherosclerosis. In this study, we examined the anti-proliferative effect and its mechanism on rat aortic VSMCs treated by kaempferol. kaempferol significantly inhibited the platelet-derived growth factor (PDGF)-BB-induced proliferation of rat aortic VSMCs in concentration-dependent manner by cell count and ［$^3$H］-thymidine incorporation assay. (omitted)

• Journal of Periodontal and Implant Science
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• 제29권3호
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• pp.507-519
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• 1999

The ultimate objective of periodontal treatment is to stop disease progression and to regenerate destroyed periodontal tissues and thereby regain normal function. Growth factors are naturally found polypetides which stimulate many cellular activities pertaining to wound healing by acting as signal molecule in controlling cell movement, proliferation, and matrix production. Platelet derived growth factor (PDGF) is 28,000-35,000 Da molecular weight dimeric protein with 2 long positively charged polypeptide chains connected by sulfide bonds. The purpose of this study is to evaluate histologically the initial guided tissue regeneration in a periodontal defect f a beagle dog treated with a biodegradable membrane formed with polylactic acid (poly-L-lactic acid) and polyglycolic acid loaded with 200ng/$cm^2$ platelet derived growth factor. 2 beagle dogs were used in he experiment. $5mm{\times}6mm$ alveolar bone defect was formed in upper and lower canines and third premolars and a reference notch was placed. PDGF-BB non-containing membrane was used as control. Each defect was randomly assigned to the test roup or the control group. The dogs were sacrificed 3 weeks after membrane placement. Toluidine blue and multiple staining was done for histological analysis. In the 3 week specimen in the control group, no new one formation could be seen. Small amount f bone resorption below the notch could be seen. In the notch, loose connective tissue with infiltration of inflammatory cells could be seen. Also thin discontinuous new cementum could be seen and the membrane still retained its structure. Where PDGF-BB containing membrane was used, new bone formation could be seen in the notch at weeks and also continuous thin cementum could be seen. PDL cells were observed between new bone and new cementum and some were attached to bone and cementum. These results suggest that new bone and cementum formation seen when PDGF-BB loaded membrane was used was due to inhibition of downgrowth of epithelial cells and also due to continuous release of the growth factor. Further study on the resorption characteristics of the membrane nd the release characteristics of the PDGF-BB is necessary. Also, development of a membrane easier to use clinically is necessary.

• 대한치과교정학회지
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• 제27권6호
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• pp.955-961
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• 1997

흡연의 주성분중의 하나인 니코틴은 인체내에 다양한 전신적 및 국소적인 질환의 원인으로 보고 되어지고 있다. 전신적인 질환에 있어 특히, 호흡기와 순환기 조직세포에 대한 세포분열효과가 많은 연구의 초점이 되어왔으며, 국소적인 효과에 대한 연구에서는 조직파괴나 치료후 치유지연에 대해 보고하고 있다. Platelet-Derived Growth Factor(PDGF)와 Insulin-like Growth Factor(IGF)는 치주인대세포의 세포분열을 촉진하는 주요 성장인자로 알려져있다. 본 연구의 목적은 니코틴이 사람의 치주인대세포에 미치는 세포분열효과를 알아보기 위하여 니코틴 처리된 치주인대세포로부터 추출한 PDGF-${\alpha}\;and\;{\beta}$ receptor 및 IGF-l 수용기의 mRNA변화를 Northern분석을 이용해 확인해 보고자 함이다. 실험군은 각기 다른 농도의 니코틴(100ng/ml, 1000mg/ml)과 배양액내 혈청농도($1\%,\;10\%$)로 나누었으며 이를 각각 니코틴 처리 시간에 따라 분류하였다. 본연구의 결과로 $10\%$ 혈청의 배양액과 100ng/ml 니코틴 농도군에서 모든 성장인자 수용체의 mRNA가 증가됨을 보였으며 이는 흡연자의 체내 축적 가능한 니코틴 농도에서 치주인대세포의 세포분열을 촉진한다는 추측을 가능케 한다.

• Journal of Periodontal and Implant Science
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• 제30권2호
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• pp.347-360
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• 2000

Bone remodeling results from the combined process of bone resorption and new bone formation which is regulated in part by some of the polypeptide growth factors such as platelet derived growth factor(PDGF), which has been known to be an important local regulator of bone cell activity and participate in normal bone remodeling. This process includes strictly regulated gene expression of several bone matrix proteins such as type I collagen and osteopontin, a 44 kDa phosphorylated glycoprotein, which has important roles in bone formation. The purpose of this study is to evaluate the effecs of PDGF-BB on the mRNA expression of bone matrix protein, type I collagen and osteopontin, in MC3T3- E1 cell culture. Cells were seeded at $5{\times}10^5$ cells in 10 ml of minimum essential medium alpha(${\alpha}-MEM$) containig 10% fetal bovine serum, 10 mM beta glycerophosphate. 0.1, 1, 10 ng/ml PDGF-BB were added to the cells for the day 3, 7, 14, 21, 28 and cultured for 24 hours. Type I collagen cDNA, Hf677, and osteopontin cDNA were used as probes for northern blot analysis. Total cellular RNA was purified at indicated day and northern blot analysis was performed. The results were as follows : Type I collagen mRNA expressions were higher at the day 3 and 7, and lower in the day 14, 21 in the control groups. In the experimental groups, mRNA expressions were increased when 0.1 ng/ml PDGF-BB were added on the day 3, 7, 21, and decreased in dose-dependent manner on the day 14, decreased at all added dose on the day 28. Osteopontin mRNA expressions were highest in the day 21 groups and lowest in the day 14 groups in the control groups. Interesting results were shown in the day 14 and 21 groups. We found that osteopontin mRNA level was increased in dose dependent manner in the day 14 groups, and decreased dose dependent manner in the day 21 groups. In conclusion, PDGF-BB may have various control effects on type I mRNA expression in the growth and differentiation process of MC3T3-E1 cells and may have contrary regulatory effects on osteopontin mRNA expression. For examples, when the baseline level of osteopontin mRNA was low, as in the day 14, PDGF-BB up-regulated osteopontin mRNA expression in dose dependent manner, and when the baseline level was high as in the day 21, PDGF-BB down-regulated dose dependent manner. Thus, it may be useful for clinical application in periodontal regeneration procedure if further study were performed.