• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.311-328
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• 2017

This study was carried out to investigate the changes in soil microorganisms and soil enzymes by split irrigation and organic matter application under no-tillage green house conditions. Soil bacteria and fungi abundances were higher in soybean cake fertilizer than in the soil without the soybean cake fertilizer under whole quantity irrigation. Bacteria and fungi abundances in soil increased with increasing organic fertilizer application rate. Bacteria and fungi amount in the soil increased at half division irrigation in no-treatment of soybean cake fertilizer compared with whole quantity irrigation. Actinomycete amount in the soil decreased with increasing soybean cake fertilizer with whole quantity irrigation while clearly increased in no-treatment of soybean cake fertilizer. Actinomycete amount in soil clearly increased with increasing organic fertilizer input at half division irrigation. Chitinase activity in the soil decreased in soybean cake fertilizer with increasing organic fertilizer input, while increased in no-treatment of soybean cake fertilizer. Chitinase activity in the soil increased at half division irrigation compared with whole quantity irrigation regardless of soybean cake fertilizer input. ${\beta}$-Glucosidase activity in the soil was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. ${\beta}$-Glucosidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. ${\beta}$-Glucosidase activity in the soil clearly increased in no-treatment of soybean cake fertilizer at half division irrigation compared with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil was not significantly different at half division irrigation and whole quantity irrigation in organic fertilizer input, while increased at half division irrigation in no-treatment of soybean cake fertilizer. Acid phosphatase activity increased at standard level 66% in soybean cake fertilizer, while was not significantly different in no-treatment of soybean cake fertilizer. Spore density of Arbuscular Mycorrhizal Fungi (AMF) in the soil increased with increasing organic fertilizer input at whole quantity irrigation in no-treatment of soybean cake fertilizer, while decreased above the standard level 66% in organic fertilizer input. However, spore density of AMF in the soil was not significantly different in soybean cake fertilizer regardless of input amount of organic fertilizer. Root colonization rate of AMF in red pepper roots was not significant difference at two irrigations regardless of soybean cake input.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1230-1236
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• 2012

To verify that the electrokinetic remediation is effective for decreasing salinity of fields of the plastic-film house, field tests for physical property, chemical property, and crop productivity of soils have been conducted. The abridged result of those tests is as follows. In the EK treatment, the electrokinetic remediation has been treated at the constant voltage (about 0.8 V $cm^{-1}$) for fields of the farm household. At this time, an alternating current (AC) 220 V of the farm household was transformed a direct current. The HSCI (High Silicon Cast Iron) that the length of the stick for a cation is 20cm, and the Fe Plate for an anion have been spread out on the ground. As the PVC pipe that is 10 cm in diameter was laid in the bottom of soils, cations descend on the cathode were discharged together. For soil physical properties according to the EK treatment, the destruction effect of soil aggregate was large, and the infiltration rate of water was increased. However, variations of bulk density and porosity were not considerable. Meanwhile, in chemical properties of soils, principal ions of such as EC, $NO_3{^-}$-N, $K^+$, and $Na^+$ were better rapidly reduced in the EK treated control plot than in the untreated control plot. And properties such as pH, $P_2O_5$ and $Ca^{2+}$ had a small impact on the EK. For cropping season of crop cultivation according to the EK treatment, decreasing rates of chemical properties of soils were as follows; $NO_3{^-}$-N 78.3% > $K^+$ 72.3% > EC 71.6% $$\geq_-$$ $Na^+$ 71.5% > $Mg^{2+}$ 36.8%. As results of comparing the experimental plot that EK was treated before crop cultivation with it that EK was treated during crop cultivation, the decreasing effect of chemical properties was higher in the case that EK was treated during crop cultivation. After the EK treatment, treatment effects were distinct for $NO_3{^-}$-N and EC that a decrease of nutrients is clear. However, because the lasting effect of decreasing salinity were not distinct for the single EK treatment, fertilization for soil testing was desirable carrying on testing for chemical properties of soils after EK treatments more than two times. In the growth of cabbages according to the EK treatment, the rate of yield increase was 225.5% for the primary treatment, 181.0% for the secondary treatment, and 124.2% for third treatment compared with the untreated control plot. The yield was increased by a factor of 130.0% for the hot pepper at the primary treatment (Apr. 2011), 248.1% for the lettuce at the secondary treatment (Nov.2011), and 125.4% for the young radish at the third treatment (Jul. 2012). In conclusion, the effect of yield increase was accepted officially for all announced crops.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.77-101
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• 2009

Organic farming in Korea has mainly focused on producing vegetables in plastic film house and cereals in paddy field. For high productivity of vegetables and cereals, most Korean farmers have not applied crop rotation in the cropping system. Thus, this study was carried out to evaluate the effects of crop rotation on the yield of red pepper and green onion, the changes in soil fertility and the potential as green manure of rye and hairy vetch. Rye and hairy vetch were cultivated for winter season every year, and directly incorporated into the soil. The yield of red pepper fruits in organic farming using crop rotation (OFCR) decreased 23 to 36% compared with conventional farming system (CFS). Whereas, green onion showed the increased yield of about 13%. In OFCR, total carbon content of soil was higher, however available phosphate content lower than conventional farming. As a result of measuring the bulk density of soil, OFCR and CFS were 1.26 to $1.35Mg/m^3$ and 1.37 to $1.42Mg/m^3$, respectively. Carbon and nitrogen contents of microbial biomass in OFCR were obviously higher compared with the CFS. In the plot cultivated rye for winter season, the weed germination was strongly reduced (about 52 %). Balance of macro nutrient elements, nitrogen and phosphate, in the application of rye and hairy vetch had a minus value. For potassium, the output value was higher than the input one, therefore organic farming under red pepper-rye or hairy vetch systems requires applying additional potassium input. Also, we selected two hairy vetch varieties of cv. Hungvillosa and Ostsaat which can be adapt at Korea climate. In order to estimate a yield of green manures, the weight of shoot and root was measured. The ratio of shoot and root between rye and hairy vetch showed 0.88 and 1.91, respectively. The potential levels of nitrogen, phosphate and potassium which could be supplied from rye were 7.7, 7.8 and 21.9 kg/10a and from hairy vetch were 17.0, 8.6 and 22.9 kg/10a, respectively. So we recommend that cultivating hairy vetch, as a nutrient supplier, and rye, as an amendment to enhance the soil physical property, for winter season be practical method in Korea organic farming system.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.343-348
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• 2008

To investigate the effects of incorporation of green manures (GM) into a sandy loam soil on growth, yield, and nutrient uptake of tomato (Lycoperiscon esculentum Mill.) and nutrient balances (input minus offtake of nutrients), five tomato production systems were compared under the condition of plastic film house: 1) a no input system (no additional amendment or inputs, 0-To-0-To); 2) a conventional system (application of N-P-K chemical fertilizers, Cf-To-Cf-To); 3) a leguminous GM-containing system (hairy vetch-tomato-soybean-tomato, Hv-To-Sb-To); 4) a graminaceous GM-containing system (rye-tomato-sudan grass-tomato, Ry-To-Sd-To); and 5) system mixed with leguminous and graminaceous GMs (rye-tomatosoybean- tomato, Ry-To-Sb-To). Here, hairy vetch and rye were cultivated as winter cover crops during late $Dec{\sim}late$ Feb and soybean and sudan grass were cultivated as summer cover crops during late $Jun{\sim}mid$ Aug. All of them cut before tomato planting and then incorporated into soil. Biomass of GMs was greater in summer season than that of winter season. Nitrogen amount fixed by a leguminous plants was about $126\;kg\;ha^{-1}$ per a cropping season, corresponding to 60% N level needed for tomato production, which was comparable to 50 and $96\;kg\;ha^{-1}$ fixed by rye and sudan grass. As a result, tomato yield of Hv-To-Sb-To system (legume GM treatment) was similar to Cf-To-Cf-To (conventional), but that in Ry-To-Sd-To system (graminaceous GM treatment) was not attained to a half level of conventional treatment. Nutrient budgets for N, P and K on the conventional farm were balanced or somewhat positive exception for minus-balanced K. Ry-To-Sd-To system showed a positive N, P and K budgets due to the depressed growth of tomato which is caused by high C/N ratio and low N-fixing capacity of the GMs. Inversely, those of Hv-To-Sb-To system were negative in all of N, P and K budgets because of increased growth and yield of tomato with high nitrogen-supplying capacity as well as low C/N ratio of leguminous GM. In conclusion, although conventional cultivation has an advantage in relation to N, P and K nutrient budgets rather than GM-incorporated systems, a leguminous GMs could be recommended as nitrogen reservoir and soil amendment because the yield of tomato between use of leguminous GM and conventional cultivation was not only significantly difference, but also GMs commonly reduce nutrient loss and improve microbial communities.

• Korean Journal of Environmental Agriculture
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• v.24 no.1
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• pp.24-28
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• 2005

Waste of the hydroponic solution from the plastic film house cultivation was recycled to grow the red pepper(Capsicum annum L.) in upland fields as supplement for plant nutrients and irrigation sources. Application of hydroponic waste solution increased the pH and EC of the soils, coupled with the increases in the concentrations of exchangeable cations(Ca, Mg, and K), total nitrogen, $NH_4-N,\;and\;NO_3-N$. Growth and yield of red pepper were highest when the treatment of chemical fertilizer(70%) was combined with hydroponic waste solution(30%). Amounts of the daily producing hydroponic waste solution were 2,880 L $ha^{-1}\;day^{-1}$ from the experimental facilities and this could irrigate $409.86m^2$ of area to compensate for the amount of water loss by evapotranspiration(3%). The overall results demonstrated that hydroponic waste solution could be recycled as plant nutrients and irrigation water resources for enhancing soil fertility and environmental quality.

• Korean Journal Plant Pathology
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• v.3 no.3
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• pp.187-197
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• 1987

Bacteria and fungi antagonistic to Fusarium oxysporum f. sp. cucumerinum Owen were effectively isolated with each of modified Triple Layer Agar (TLA) technique from rhizosphere soil where cucumber had been grown healthily in plastic film house. Three predominant bacterial isolates selected were identified as Pseudomonas fluorescens, and P. putida, Serratia sp. and three fungal isolates were Gliocladium sp. Trichoderma harzianum, and T. viride. Antagonistic bacteria inhibited $26-45\%$ of germination and $41-56\%$ of germ tube elongation of microconidia of F. oxysporum f. sp. cucumerinum on Water Agar (WA). P. fluorescens was the strongest inhibitor. Several my co parasitisms were observed on dual culture of WA between antagonistic fungi and F. oxysporum f. sp. cucumerinum such as coiling, penetration, overgrowing, and lysis. Mycelial lysis of the pathogen was the most severe at pH 4.6, followed by 3.6, 5.6 and 6.6 of the medium in decreasing order. At pH 6.6, mycelia of the pathogen were not conspicuously damaged, however, the antagonistic fungi formed abundant chlamydospores especially Gliocladium sp. T. harzianum revealed the most excellent antagonism in vitro.

• Korean Journal of Environmental Biology
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• v.39 no.2
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• pp.147-155
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• 2021

To investigate the incidence of plant-parasitic nematodes (PPNs) in perilla fields in Korea, 55 soil samples were collected from open fields and plastic-film house fields and were analyzed during January 2020 to October 2020. Root-lesion nematodes (RLNs), spiral nematodes, root-knot nematodes, and stunt nematodes were detected in perilla fields, and the incidences of RLNs (39%) and spiral nematodes (55%) were higher than those of other nematodes. Among PPNs, RLNs are very important species found on economic crops. The detection frequency of RLNs was increased due to continuous crop cultivation, and the frequency of fields that cultivated only one crop continuously for over 11 years was twice higher than the frequency of fields that cultivated only one crop for less than 5 years. The PPN species diversity and density were different between leaf-perilla cultivation fields and seed-perilla cultivation fields. In phylogenetic analysis of RLNs, Pratylenchus penetrans, P. vulnus, and Pratylenchoides leiocauda were identified in perilla fields. These results suggest the RLNs should be considered for establishing nematode management strategies in perilla fields in Korea.

• Korean Journal of Organic Agriculture
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• v.30 no.2
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• pp.279-290
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• 2022

This study was conducted to find out that winter green manure crops could be efficient replacements of expeller cake fertilizers, which were mostly imported, on environment-friendly red pepper production. Four treatments were compared under the condition of plastic film house: 1) barley (B); 2) hairy vetch (HV); 3) mixtures of barley and hairy vetch (B/HV); 4) expeller cake (EC). Total nitrogen content in hariy vetch was 3.6%, which was higher than 1.5~1.8% in barley and mixtures of barley and hairy vetch. P₂O₅ and K₂O contents were similar in all green manures. Supplying amount of nitrogen from B, HV and B/HV plot, which were 172 kg ha^-1, 193 kg ha^-1, and 198 kg ha^-1, were higher than amount of basal nitrogen required by soil testing but were lower than that of total nitrogen, respectively. Among the green manure crops, C/N ratio of HV was the lowest at 11.8, showing a similar value to that of expeller cake, but that of B was the highest at 30.6. Total yield was no significant difference in all treatment plots although B/HV plot showed the highest yield, but initial yield in B plot with high C/N ratio was lower than that in EC plot.

• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.731-742
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• 2014

In the current study, we investigated effects of a combination of earthworm casting, environment-friendly by-product fertilizer, and cultivation soil of Gymnocalycium mihanovichii in a heavy fertilizing culture on diameter, height, numbers of tubercles, and chemical properties of soil thereby elucidating optimal mixture ratio for securing production as well as providing nutrients throughout cultivation period. The Gymnocalycium mihanovichii var 'Ihong', one of grafted cactus for export (Rootstock: 9 cm, Scion: $1.5{\times}1.3cm$ grafted cactus) was cultured in plastic houses of Agricultural Technology Center located in Naegok-dong, Seocho-gu, Seoul from June, 2013 through December, 2013. For the control group, a mixture of sand and fertilizer (50:50) was used as this ratio is widely utilized in farmhouses. In contrast, a variety mixtures of sand and earthworm casting that was produced with food wastes was compared; the mixture ratios were 80:20, 60:40, 40:60, 20:80, and 0:100 and pH for these mixtures were found to be similar each other (ranging between 7.1 and 7.4) which is in an appropriate range (pH 6.5-7.5) for cultivation of G. mihanovichii. The organic content was increasing along with increasing contents of earthworm casting ratio while it was lower than the treatment practice group (32-43 mg/kg vs. 55 mg/kg). The content of exchangeable cation was also increasing as the ratio of earthworm casting was elevated; although levels of $K^+$, $Na^+$, and $Mg^{2+}$ were lower than the treatment practice group, the level of $Ca^{2+}$ was higher ($9.1cmol^+/kg$ and $11.5-33.7cmol^+/kg$ in the treatment practice group and the earthworm casting group, respectively). Three months after grafting, diameters of G. mihanovichii were compared with the control group; consequently, there was a significant difference noted in between the earthworm casting group and the control group (31.39 mm vs. 32.46-37.59 mm). After 5 months, growth characteristics of G. mihanovichii were evaluated. Similarly, the diameter of G. mihanovichii was significantly increasing in the group with higher ratio of earthworm casting treatment (32.63 mm vs. 32.49-37.59 mm). The height of tubercles was 2.63 mm in the control group while it was significantly elevating along with the ratio of earthworm casting mixture. The more numbers of tubercles, the more incomes for farm-houses; as results, higher mixture ration of earthworm casting resulted more numbers of tubercles compared to the control group (2.7 vs. 3.2-8.3 ea). In particular, in the earthworm casting groups with 80% and 100% ratios, the numbers of tubercles were 6.2 and 8.3 ea, respectively, which is 2.5 times more than those of the control group. These results indicate that earthworm casting treatment may be utilized in G. mihanovichii farming houses for short term production of tubercles. In the group with 40% and 60% of earthworm casting mixture, the numbers of tubercles were found to be 4.5 and 4.8 ea, respectively which is higher than the control group as well; in these groups, there were no issues with soil drainage as well as moss formation. Given the analysis results of growth characteristics of G. mihanovichii, it was concluded that 40% and 60% of earthworm casting mixture might be the optimal ratios.

• Korean Journal of Environmental Agriculture
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• v.26 no.4
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• pp.306-312
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• 2007

To investigate the effect of N fertigation on the growth, yield, and water and nitrogen use efficiencies during tomato cultivation, seedlings were transplanted in a sandy loam soil under plastic film house condition. 0, 88, 132, 176, $220\;kg\;ha^{-1}$ N rates, which correspond to 0 (NF0), 40 (NF40), 60 (NF60), 80 (NF80), 100% (NF100) N level of soil test-based N fertilization, were injected weekly through drip irrigation system for 15 weeks in N fertigation system, and the control (conventional N treatment) was installed for comparison. Herein, nitrogen was applied by top-dressing with 60% as a basal and 40% as additional fertilizer. There was little different in stem diameter growth among N fertigation treatments, but plant height and dry matter increased with increasing N fertigation rates as well as in N conventional treatment. Tomato yield was increased with increasing the number of marketable fruits in N fertigation treatments, and the fruit yield was maximized in NF 80 treatment ($176\;kg\;ha^{-1}$ N supply or $96.6\;mg\;L^{-1}$ N injection). Dry matter productivity and nitrogen uptake amount were significantly increased with increasing N fertigation rates. The ratio of fruits to the dry weight of whole plant was decreased with increasing N fertigation rates, but this ratio was $2.6{\sim}5.3%$ higher in N fertigation treatments than in the control. In addition, the ratios of nitrogen distributed toward fruits in N fertigation treatments were $3.7{\sim}21.7%$ higher than that of control. The apparent N recovery percentages showed significantly higher values as $71.8{\sim}102.3%$ in N fertigation treatments, compared to 45% in N conventional treatment. Water use efficiency was significantly increased by fertigation system with the maximum $361\;kg/ha\;cm^{-1}$ in NF 80, which is comparable to $324\;kg/ha\;cm^{-1}$ of the conventional treatment. Conclusively, N fertigation system was effective on increasing tomato productivity and nutrient efficiency as well as 20% reduction of N fertilization level.