• Title/Summary/Keyword: Plasma

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Physiological and Biochemical Response of Blood on Low Temperature Stress in Sevenband Grouper, Epinephelus septemfasciatus (저수온 스트레스에 따른 능성어 (Epinephelus septemfasciatus) 혈액의 생리생화학적 반응)

  • Park, Jong Youn;Park, Jae Min;Hong, Chang Gi;Kim, Kyong Min;Cho, Jae Kwon
    • Korean Journal of Ichthyology
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    • v.28 no.1
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    • pp.1-8
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    • 2016
  • This study was conducted to investigate the physiological responses of sevenband grouper, Epinephelus septemfasciatus at low temperature condition in winter season. Water temperature was dropped from $12^{\circ}C$ to $5^{\circ}C$ by two different interval (interval I: $-1^{\circ}C/24h$ and interval II: $-1^{\circ}C/8h$). The experimental fishes were exposed for 9 days at each water temperature condition, and were observed survival rate. The blood sample was collected from fishes for analysis of cortisol, glucose, total protein (TP), and electrolytes ($Na^+$, $Cl^-$, $K^+$). Mortality of fish was not observed until $5^{\circ}C$. The mortality (12 individuals) began after 170 h and all fish were death after 174 h in interval I case, whereas the mortality (9 individuals) began after 72 h and all fish were death after 76 h in interval II case. Little different was observed in the physiological responses, but trend was similar between two intervals. The physiological metabolism of plasma cortisol and glucose were increased rapidly, and swimming ability and respiration response was faster at $7^{\circ}C$. This result means that the critical temperature of sevenband grouper was $7^{\circ}C$ at low water temperature condition.

Fabrication and sintering of nano $TiN_x$ and its composites (Nano $TiN_x$와 그 복합체의 제조 및 소결)

  • Kim, Dong-Sik;Kim, Sung-Jin;Rahno, Khamidova;Park, Sung-Bum;Park, Seung-Sik;Lee, Hye-Jeong;Lee, Sang-Woo;Cho, Kyeong-Sik;Woo, Heung-Sik;Ahn, Joong-Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.16 no.3
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    • pp.101-105
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    • 2006
  • We fabricated the nano $TiN_x$ by making of reaction between titanium powder and $Si_3N_4$ during planetary milling. The $TiN_x$ powder was sintered by spark plasma sintering machine after mixing with 50 wt% of titanium powder, and the sintered body was heat-treated at $850^{\circ}C$ in order to investigate its hardness property at the elevated temperature. We analyzed crystal structure by XRD. We observed the peaks of $TiN_{0.26}$ and TiN after 10 hours milling, and we observed TiN peak mainly after 20 hours milling. The reacted particle size distribution was investigated by FE-SEM. Increase of milling time, the size of reacted particles was decreased and the $10{\sim}20nm$ size of $TiN_x$ on the surface of titanium and $TiN_x$ was observed after 20 hours milling. The micro-Vickers hardness of mixed sintered body was about $1050kgf/mm^2$.

A license plate detection method based on contour extraction that adapts to environmental changes (주변 환경 변화에 적응하는 윤곽선 추출 기반의 자동차 번호판 검출 기법)

  • Pyo, Sung-Kook;Lee, Gang-seong;Park, Young-Soo;Lee, Sang-Hun
    • Journal of the Korea Convergence Society
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    • v.9 no.9
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    • pp.31-39
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    • 2018
  • In this paper, we proposed a license plate detection method based on contour extraction that adapts to environmental changes. The proposed method extracts contour lines using DoG (Difference of Gaussian) to remove unnecessary noise parts in the contour extraction process. Binarization was applied in ugly outline images, and erosion and dilation operations were used to emphasize the contour of the character part. Then, only the outline of the ratio of the characters of the plate was extracted through the ratio of the width and height of the characters. And the case where the outline is the longest is estimated by estimating the characters of the license plate. For the experiment, we applied 130 image data to license plate on the front of the vehicle, oblique environment, and environment images with various backgrounds. I also experimented with motorcycle images of different license plate patterns. Experimental results showed that the detection rate of the oblique image was 93% and that of the various background environment was 70% in the motorcycle image but 98% in the front image.

Crystallization Kinetics by Thermal Analysis (DTA) on Starting Glass Compositions for PDP(Plasma Display Panel) Rib (열분석에 의한 PDP 격벽용 출발유리조성의 결정화 특성 연구)

  • Jeon, Young-Wook;Cha, Jae-Min;Kim, Dae-Whan;Lee, Byung-Chul;Ryu, Bong-Ki
    • Journal of the Korean Ceramic Society
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    • v.39 no.8
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    • pp.721-727
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    • 2002
  • In order to overcome trade-off among compositions, process and properties of the glasses with high PbO-base composition for PDP Rib, we studied glass crystallization and crystallization kinetics by Differential Thermal Analysis(DTA). Glass powder was obtained through melting/cooling/grinding, with 3 wt%TiO2 addition for the crystal nucleation and growth in $62PbO-19B_2O_3-10SiO_2-9(Al_2O_3-K_2O-BaO-ZnO)$(in wt%) composition glass. This powder was heat-treated for 1 to 10 h at $445^{\circ}C$ for nucleation. DTA measurements were performed to obtain the crystallization peak with $5∼25^{\circ}C/min$ heating rates. DTA crystallization peak temperature increased with increasing the heating rate and decreased with increasing the heating time. Because the Avrami parameter (n) was approximately 1, the surface crystallization occurred. The maximum nucleation time was 2 h.

Localization of Dendroaspis natriuretic peptide (DNP) in the rat salivary glands (흰쥐 타액선내에서의 DENDROASPIS NATRIURETIC PEPTIDE(DNP)의 분포)

  • Kim, Jae-Gon;Lee, Young-Soo;Baik, Byeong-Ju;Park, Byung-Keon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.28 no.3
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    • pp.447-463
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    • 2001
  • Dendroaspis natriuretic peptide (DNP), a fourth member of the natriuretic peptide isolated from the venom of the Dendroaspis angusticeps snake, has been reported to be present in human plasma and atrial myocardium and caused vasorelaxation and diuresis in experimental animals. However, it is uncertain whether they are present in peripheral organs other than the heart and its further physiological roles also remains to be clarified. To assess the possible physiological role of DNP in the salivary glands, I investigated the localization of DNP peptide in the rat salivary glands by immunohistochemistry and the binding sites for radiolabelled DNP in the rat salivary glands and oral mucosa using in vitro autoradiography. DNP immunoreactivity was widely distributed in the submandibular, sublingual and parotid glands, particularly in the ducts such as the intercalated and striated ducts, where atrial natriuretic peptide (ANP) was colocalized in consecutive sections, but not in acini. High density $^{125}I-DNP$ binding sites were localized in the epithelia of the tongue and hard palate, while low density binding sites for $^{125}I-DNP$ were also distributed in the submandibular, sublingual, and parotid glands. In the hard palate and tongue, the precise location of this binding was revealed on the basal and parabasal cells of the epithelia by emulsion microautoradiography. These results suggest that DNP may not only have a role in the salivary glands but also play a role in the regulation of growth in the oral epithelium, particularly in the hard palate and tongue.

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Rhus verniciflua Extract Ingestion and Exercise Training on Blood Lipids and Insulin Resistance in Rats (옻나무 추출액 섭취와 운동훈련이 흰쥐의 혈중 지질 및 인슐린 저항성에 미치는 영향)

  • Lee, Youn-Kyung;Lee, Soo-Chun;Jeon, Byung-Duk;Kim, Sea-Hyun;Kim, Pan-Gi;Ryu, Sungpil
    • Journal of Korean Society of Forest Science
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    • v.98 no.6
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    • pp.681-689
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    • 2009
  • We investigated the effect of Rhus verniciflua Stokes extract ingestion with exercise training on plasma lipids and insulin resistance for 8 weeks. Forty SD (Sprague-Dawley) male rats were used as the experimental animals that were divided into CON (control), RVS (Rhus verniciflua Stokes), EXE (exercise training), and RVS-EXE (Rhus verniciflua Stokes with exercise training), respectively. Body weight gain in EXE (202%) and RVS-EXE (203%) was significantly lower than CON (253%) and RVS (239). Stored fats were significantly lower with RVS and/or exercise training. Blood lipids were enhanced in RVS and RVS-EXE compared to CON. Blood glucose was significantly high in CON compared to the other groups. Insulin and HOMA index has the same tendencies with glucose, however, the synergic effect was found in RVS-EXE. In conclusion, Rhus verniciflua Stokes extract ingestion with exercise training has the effect of lowering amount of stored fats, reducing blood lipids, and enhancing insulin resistance, therefore, metabolic syndrome, diabetes, obesity, and hyperlipidemia might be prevented.

Effect of Antioxidative Vitamin Supplementation on Mineral Contents in the Hair and Autistic Related Behaviors in Autistic Children (자폐아동에 있어서 항산화 영양소 보충이 모발의 미네랄 함량 및 자폐성향에 미치는 영향)

  • Song, Won-Young;Hong, Jung-Hee;Park, Eun-Ju;Lee, Han-Woo;Choi, Jeong-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.2
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    • pp.237-243
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    • 2010
  • This study was investigated the effect of antioxidative vitamin supplementation ($\beta$-carotene 175 mg, vitamin C 200 mg, vitamin E 33.5 mg) for 12 weeks on antioxidant status and autistic related behaviors in autistic children. The antioxidative vitamin-supplemented children had significantly lower concentrations of toxic mineral, such as cadmium (Cd), mercury (Hg) and lead (Pb) in hair, while they had higher concentration of antioxidant minerals (Se, Zn, Fe) in hair. Antioxidant vitamin supplementation for 12 weeks reduced free radical while it increased the hair levels of serotonin compared to the beginning of the study. Additionally, antioxidative vitamin supplementation resulted in significant improvement in social age. These results indicate that the consumption of antioxidative vitamin supplementation for 12 weeks in autistic children may increase plasma antioxidant mineral concentration, resulting in reduced toxic mineral and free radical and improved autistic behavior.

Association between Dietary Sodium Intake and Abdominal Obesity in Pre-diabetes Korean Adults (전당뇨병 성인에서 나트륨 섭취와 복부비만과의 상관관계)

  • Lim, So Young;Yang, Soo Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.5
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    • pp.763-771
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    • 2014
  • The objective of this study was to assess the relationship between dietary sodium intake and prevalence of abdominal obesity in Korean adults. We used data from the Korea National Health and Nutrition Examination Survey V-1 and analyzed data on 4,475 Koreans (${\geq}30$ years old). Subjects were divided into three groups according to fasting plasma glucose (FPG): 1) normal (FPG <100 mg/dL), 2) pre-diabetes ($100mg/dL{\leq}FPG{\leq}125mg/dL$), and 3) diabetes (FPG ${\geq}126mg/dL$ or subjects diagnosed with diabetes). The subjects in each category were stratified by dietary sodium intake as well as index of abdominal obesity. We found that dietary sodium intake was positively correlated with waist circumference (WC) (P=0.002) and was particularly high in the pre-diabetes group. In multiple logistic regression analysis, the normal and diabetes groups showed no association between dietary sodium intake and WC, whereas the pre-diabetes group with a high sodium intake exhibited a significant association (odds ratio (OR)=1.479, P=0.029) between dietary sodium intake and WC. Further, the OR for abdominal obesity in the high sodium intake group with pre-diabetes was 1.590 after adjusting for age and sex (P=0.012). In addition, the ORs for the prevalence of abdominal obesity with fasting glucose, fasting insulin, and homeostasis model assessment of insulin resistance were significantly higher in the pre-diabetes group with high sodium intake compared with low sodium intake. Moreover, these associations were significant even after adjusting for confounding variables (model 2: age and sex; model 3: age, sex, and total energy intake). Our results suggest a strong association between sodium intake and abdominal obesity in pre-diabetes Korean adults.

Optimal Incorporation Level of Dietary Alternative Phosphate (MgHPO4) and Requirement for Phosphorus in Juvenile Far Eastern Catfish (Silurus asotus)

  • Yoon, Tae-Hyun;Lee, Dong-Hoon;Won, Seung-Gun;Ra, Chang-Six;Kim, Jeong-Dae
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.111-119
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    • 2015
  • A growth trial was conducted to determine the optimal incorporation level of dietary magnesium hydrogen phosphate (MHP, $MgHPO_4$), which was manufactured from swine manure and phosphorus (P), required by juvenile far eastern catfish (Silurus asotus). Graded MHP of 0.5%, 1.0%, 1.5%, and 2.0%, and 2.0% monocalcium phosphate (MCP) each was added to the basal diet (control) in lieu of cellulose to become the range of available P (AP) from 0.4% to 0.8% of which diets were designated as control, MHP0.5, MHP1.0, MHP1.5, MHP2.0, and MCP, respectively. Control diet contained fish meal (20%), soybean meal (40%), wheat flour (27%), corn gluten meal (5%), fish oil (2%) and soy oil (2%) as main ingredients. Following a 24 h fasting, 540 fish with a mean body weight of 11.8 g were randomly allotted to 6 groups in triplicate, whereby 18 tanks ($0.4{\times}0.6{\times}0.36cm$, water volume of 66 L) were prepared. The feeding experiment lasted for 8 weeks. Fish group fed the control diet showed the lowest weight gain (WG) and feed efficiency (FE) among treatments. The WG was, however, not significantly different (p>0.05) from that of fish group fed MHP0.5. Fish group fed MHP2.0 showed the highest WG and FE of which values were not significantly different from those of fish groups fed diets MHP1.0 and MHP1.5 as well as MCP (p>0.05) except fish groups fed control and MHP0.5. Aspartate aminotransferase was significantly decreased with an increase in available P, while alanine aminotransferase did not show a significant difference among treatment. The highest inorganic P in plasma was observed in fish fed MHP2.0. From the present results, a second-order regression analysis revealed that the optimal dietary MHP level and the AP requirement were found to be 1.62% and 0.7%, respectively.

Concentrations of Progesterone and Estradiol in Peripheral Plasma during the Estrous Cycle and after Ovariectomy in Huanghuai Goats of High or Poor Prolificacy

  • Pang, X.S.;Wang, Z.Y.;Zhu, T.G.;Yin, D.Z.;Zhang, Y.L.;Meng, L.;Wang, F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.2
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    • pp.188-196
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    • 2010
  • The objective of this study was to characterize the litter sizes in Huanghuai goats with high prolificacy (HP, five or more kids born per litter on at least one occasion), and to compare their peripheral blood concentrations of progesterone and estradiol with those of goats with poor prolificacy (PP, up to three kids born per litter on any occasion). The circulating concentrations of progesterone and estradiol were measured by radioimmunoassay from daily blood samples taken during natural estrus cycles and at 1-5 days after ovariectomy. Estrus was synchronized using two doses of a prostaglandin analog. Litter size for the HP goats increased up to a parity of five and decreased thereafter. The percentage of goats with litter sizes of $\geq$4 from parities 3 to 6 ranged from 44.5% to 58.3%. Although small differences in litter size were obtained for goats of parities three, four and six relative to five, parity five does had the highest mean litter size. Progesterone concentrations began to rise earlier and were higher in the HP than in the PP goats on most days of the luteal phase, but not during the follicular phase of the cycle or after ovariectomy. There was a significant difference between the two groups (p<0.05) in the magnitude of the progesterone plateau. Mean estradiol concentrations in the HP group remained significantly higher than in the PP group (p<0.05) during the estrus cycle. There were two estradiol peaks in the HP goats during the early luteal phase, but only one in the PP group. Measurements of individual corpora lutea (CL) in vitro showed that there was a greater prevalence of small CL (<6 mm in diameter) in the HP group than in the PP group (p<0.05). After ovariectomy, the estradiol level on day 1 was significantly higher than at the nadir during the estrus cycle in both the HP (p<0.01) and PP (p<0.05) goats, while levels decreased by 12.3% and 26.2% respectively compared with the mid-luteal period in HP and PP goats (p<0.05). The overall mean estradiol concentrations in HP goats were lower than in the PP group, but no significant differences were found between groups at 1-5 days after ovariectomy.