• Journal of Periodontal and Implant Science
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• v.52 no.5
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• pp.394-410
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• 2022

Purpose: The purpose of this study was to compare the microbial composition of 3 types of oral samples through 16S metagenomic sequencing to determine how to resolve some sampling issues that occur during the collection of sub-gingival plaque samples. Methods: In total, 20 subjects were recruited. In both the healthy and periodontitis groups, samples of saliva and supra-gingival plaque were collected. Additionally, in the periodontitis group, sub-gingival plaque samples were collected from the deepest periodontal pocket. After DNA extraction from each sample, polymerase chain reaction amplification was performed on the V3-V4 hypervariable region on the 16S rRNA gene, followed by metagenomic sequencing and a bioinformatics analysis. Results: When comparing the healthy and periodontitis groups in terms of alpha-diversity, the saliva samples demonstrated much more substantial differences in bacterial diversity than the supra-gingival plaque samples. Moreover, in a comparison between the samples in the case group, the diversity score of the saliva samples was higher than that of the supra-gingival plaque samples, and it was similar to that of the sub-gingival plaque samples. In the beta-diversity analysis, the sub-gingival plaque samples exhibited a clustering pattern similar to that of the periodontitis group. Bacterial relative abundance analysis at the species level indicated lower relative frequencies of bacteria in the healthy group than in the periodontitis group. A statistically significant difference in frequency was observed in the saliva samples for specific pathogenic species (Porphyromonas gingivalis, Treponema denticola, and Prevotella intermedia). The saliva samples exhibited a similar relative richness of bacterial communities to that of sub-gingival plaque samples. Conclusions: In this 16S oral microbiome study, we confirmed that saliva samples had a microbial composition that was more similar to that of sub-gingival plaque samples than to that of supra-gingival plaque samples within the periodontitis group.

• Imaging Science in Dentistry
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• v.53 no.3
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• pp.209-216
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• 2023

Purpose: This study was conducted to compare dental plaque scores obtained through clinical examinations and various imaging techniques, as well as to assess the effectiveness of herbal and conventional toothpastes for plaque removal. Materials and Methods: Thirty volunteers were divided into 3 groups. Each group was given a different toothpaste (from 2 herbal toothpastes and a conventional toothpaste) with which to brush their teeth for 21 days. Both initially and after brushing, dental plaque samples were collected, and plaque on the buccal surfaces of anterior teeth was scored using several imaging systems after staining with a disclosing agent. Specifically, digital dental photography, intraoral digital scanning, and FluoreCam imaging were employed to capture intraoral images. The Turesky Modified Quigley-Hein Plaque Index was used for clinical examination and image analysis. Quantitative polymerase chain reaction analyses and correlational assessments between clinical examination and imaging scores were conducted before and after toothpaste use. The Shapiro-Wilk test and Pearson correlations were utilized. Results: The lowest mean value was observed in the clinical examination without staining, while the highest was obtained using the FluoreCam method. No significant change was found in the level of any microorganism assessed following toothpaste use (P<0.05), with the exception of a decrease in S. mutans levels after using conventional toothpaste (P<0.05). Conclusion: Herbal toothpaste demonstrated plaque-removal effectiveness comparable to that of conventional toothpaste. The use of imaging methods for measuring plaque index has been suggested as a means to educate patients about plaque control and promote ongoing oral care.

• The korean journal of orthodontics
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• v.40 no.2
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• pp.95-105
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• 2010

Objective: To identify clinical, salivary, and bacterial changes during orthodontic treatment with follow-up to 24 months. Methods: In 30 patients, clinical (decayed, missing, and filled surfaces [DMFS], O'Leary's plaque index, and plaque pH), salivary (unstimulated and stimulated saliva, buffer capacity, pH, and occult blood), and bacterial (Streptococcus mutans and Lactobacillus) markers were evaluated. A questionnaire was employed to evaluate their hygienic-dietary habits. Data were analyzed by ANOVA, logistic regression and Spearman's correlation. Results: DMFS increased significantly, whereas the plaque index diminished, plaque pH was more acidic (p = 0.23), and unstimulated salivary flow showed significant differences during the treatment (p = 0.013). Stimulated saliva flow increased in females after the placement of appliances; buffer capacity was diminished in males during the therapy; salivary pH remained at basal values. Bacterial levels and occult blood increased to high-risk levels and were not statistically significant different between genders (p > 0.05). Two major relationships were confirmed: initial plaque with use of dental aids (r = 0.429; p = 0.018) and final DMFS with unstimulated salivary flow (r = -0.372; p = 0.043). Conclusions: The increase in retentive surfaces increased the bacterial levels, plaque pH became acidified, and gingival damage was greater. Buffer capacity was altered but maintained a healthy salivary pH during the treatment.

• Journal of the korean academy of Pediatric Dentistry
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• v.7 no.1
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• pp.17-20
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• 1980

The purpose of this investigation was to make a comprehensive study and evaluation of the oral hygiene status by considering dental plaque index in 60 children age 7-9. The obtained results were as follows 1) Average plaque index of total mandibular teeth was higher than that of maxillary teeth. (Mandible; 2.14, Maxilla; 1.98) 2) In average plaque index per tooth surface, plaque index of facial surface was higher than that of lingual surface in maxilla and lower in mandible. 3) In mixed dentition, dental plaque occur most frequently and in greater quantity on the buccal surfaces of the maxillary permanent 1st molars and the lingual surfaces of the mandibular anterior permanent incisors.

• The Journal of the Korean dental association
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• v.15 no.4
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• pp.305-308
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• 1977

Dental plaque has been considered as one of the major causes of inflammation. Therefore, removal of dental plaque by tooth brushing is the most important to prevent gingival and periodontal diseases and to maintain postoperative gingival health. The author executed clinical examination on 100 outpatients who were admitted to infirmary of dental college, S.N.U. The patients were examined on their tooth brushing method, frequency, habit and admitted experience to dental clinic. The obtained results were as follows; 1. The patient group who brushed with the method of Stillman, showed the lowest plaque index. 2. The examined group who brushed more frequently per a day, showed the tendency of reduced plaque index. 3. Plaque index was lowest in the group who brushed after meals and before bed. 4. The patient group who visited more frequently to dental clinic, showed the lower tendency of plague index. 5. Among the total examined group, those who recognized the importance of oral hygiene were less than 10%.

• Journal of Periodontal and Implant Science
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• v.33 no.3
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• pp.475-484
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• 2003

Many tooth cleansing instruments and agents have been developed for removal of plaque, inhibition of plaque formation and reduction of gingival inflammation. The aim of this study was to evaluate the plaque control effect and the therapeutic effect of newly developed tooth cleansing instrument(Belloblanco(R)). 40 healthy subjects with gingivitis or early periodontitis were divided into two groups. Subjects in control group only used manual tooth brushing and in experimental group used manual tooth brush and additive tooth cleansing instrument(Belloblanco(R)). Additive tooth cleansing instrument was used once a week. At baseline scaling and tooth brushing instruction was performed. Probing depth, bleeding on probing, plaque index, gingival index were scored at baseline, 2weeks, 4weeks. Probing depth of control and experimental group were significantly reduced at 2 weeks, 4weeks, hut there were no differences between two groups(P<(0.05). Bleeding on probing, plaque index and gingival index of control and experimental group were significantly reduced at 2weeks and 4weeks and there was significantly more reduction in the experimental group than the experimental group than the control group(p<(0.05). From these finding. it can be conclude that newly developed tooth cleansing instrument(Belloblanco(R)) are effective on the removal of plaque and the reduction of gingival inflammation

• Journal of Korean society of Dental Hygiene
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• v.18 no.5
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• pp.853-862
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• 2018

Objectives: This study involved a non-equivalent control group, pre-test/post-test measurement experiment to evaluate the efficacy of distributing plaque disclosing agents to healthy adults living in South Korea and promoting its self-application. Methods: O'Leary index, PHP index, oral health behavior intention, and oral care satisfaction were used as research tools. A notice was posted on the bulletin board from September 12 to October 15, 2016, to recruit adults aged 20-50 years. Data were analyzed using SPSS Version 22.0, and frequency analysis, Pearson's chi-squared, one-way analysis of variance (ANOVA), Kruskal-Wallis, and repeated measures ANOVA were performed. Results: The O'Leary index was significantly reduced in the group (p<0.01), and time (p<0.01) after education and width of the decrease had the highest score in the group that received education on plaque disclosing only. They were followed by the group that received education on plaque disclosing and the rolling method, the group that received education on the rolling method alone, and the control group. The PHP index significantly decreased in the group(p<0.01) and time (p<0.01), and the group time (p=0.039) after education, and width of the decrease had the highest score in the group that received education on plaque disclosing and rolling method. They were followed by the group that received education on plaque disclosing alone, the group that received education on the rolling method alone, and the control group. Conclusions: Based on the aforementioned findings, the direct use of plaque disclosing had a marked effect on self-oral care. These results suggest that the self-application of plaque disclosing could be an effective self-care method for improving the oral environment.

• Journal of dental hygiene science
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• v.15 no.3
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• pp.377-382
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• 2015

The purpose of this study was to evaluate the validity and reliability of plaque scoring system using new Qraycam (All in One Bio, Korea) device which enables plaque score without tooth disclosing. This study measured Quigley-Hein index and plaque control record by both Qraycam and disclosing agent on 64 elderly people and checked degree of congruence between the two methods. Reliability was evaluated with the mean of measured values, kappa index and intraclass correlation coefficient statistical analysis. The analysis of the plaque scores showed a high agreement between the measured values according to the method of measurement and the measured part. The mean of plaque index of anterior labial were not significantly different according to measurement method. The kappa index was higher by Qraycam and tooth disclosing method of plaque index. Therefore, it was verified that Qraycam has sufficient reliability as screening tool for plaque scoring system.

• Journal of Periodontal and Implant Science
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• v.48 no.1
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• pp.22-33
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• 2018

Purpose: The purpose of this study was to evaluate the efficacy of the modified plaque score (MPS) for assessing the oral hygiene status of periodontitis patients. Methods: A total of 116 patients were included in this study. After evaluation of the $L{\ddot{o}}e$ and Silness gingival index (GI), Silness and $L{\ddot{o}}e$ plaque index (PlI), O'Leary plaque control record (PCR), and MPS, patients were randomly assigned to either a conventional tooth brushing instruction (C-TBI) group (n=56) or a professional intraoral tooth brushing instruction (P-TBI) group (n=60). The MPS and clinical parameters were re-evaluated after scaling and a series of root planing. The convergent validity of MPS with the PlI and PCR was assessed. The measurement time for MPS and PCR was compared according to the proficiency of the examiner. Results: After root planing, the GI, PlI, PCR, and MPS improved from their respective baseline values in both groups. Three different plaque indices including the MPS, showed significant differences between the C-TBI group and the P-TBI group after root planing. The MPS showed significant concurrence with the PCR and PlI. The mean time for PCR measurement was $2.76{\pm}0.71$ times longer than that for MPS measurement after 2 weeks of training. Conclusions: MPS seems to be a practical plaque scoring system compared with the PlI and PCR. These findings suggest that repetitive plaque control combined with an easily applicable plaque index (MPS) may facilitate more effective oral hygiene education and improved periodontal health.

• The Journal of the Korea Contents Association
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• v.17 no.9
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• pp.324-330
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• 2017

The aim of this study was to make a comparison of dental plaque control and reduction of oral malodor according to hardness of detergent food. Subjects are 1 male(5.0%) and 19 females(95. 0%), the average age of 20.8 years old. The study was conducted from March 6 to April 24, 2014. Detergent foods which were selected during this experiment were cucumber, cabbage and tomato. The data were analyzed by using SPSS where the PHP Index, plaque rate, $H_2S$, $(CH_3)_2S$, Oral Gas, Expiration Gas were analyzed by Non-parametric Statistics and it was compared to the results of the compared mean whereas factors of detergent food before and after ingestion were analyzed by paired t-test. With all detergent foods, compared with the degree of control of dental plaque before and after ingestion showed a statistically significant difference between PHP index from cucumber, PHP index and plaque rate from tomato, and plaque rate from cabbage.