• The Plant Pathology Journal
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• 제25권4호
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• pp.369-375
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• 2009

The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMV-Y inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RT-PCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.

• 한국응용곤충학회지
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• 제1권
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• pp.42-46
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• 1962

(1) 위조독소(Fusaric acid)를 생성하는 참깨, 시들음병균(Fusarium oxysporun f. vasinfectum)의 배양여액이 참깨, 밀, 목화, 벼 등의 종자의 발아에 미치는 영향을 구명하기 위하여 본시험에 착수하였다. (2) 공시균의 10종의 계통의 배양여액을 첨가한 발아상에 있어서의 참깨, 목화, 밀, 벼 등의 종자의 발아상태를 조사한 결과 공시균의 몇 가지 계통의 배양여액은 이들 작물종자의 발아에 뚜렷한 경향을 주지 않지만 대부분의 계통의 배양여액은 참깨, 목화, 밀, 벼 등의 종자발아를 완전히 조지하거나 혹은 발아지연 또는발아율의 저하를 초래한다는 것을 알았다. a. 공시균의 10종의 계통중 201호균의 배양여액은 참깨, 목화, 밀, 벼 등의 종자발아를 거의 완전히 저지하고 있으며 발아를 유제하는 독성에 있어서 작물간차이는 볼 수 없었으나 이 밖의 281, 321호균 등의 배양여액은 참깨, 목화. 밀, 벼 등 종자의 발아를 억제하는 독성에 있어서 작물간 차이를 볼 수 있었다. b. Fusarium oxysporum f. vasinfectum의 첩양여액이 종자발아를 억제하는 독성에 대하여 참깨가 가장 감수성이 강하고 벼는 감수성이 가장 약하며 밀, 목화 등의 감수성은 참깨와 혀의 그것의 중간에 속한다. (3) Fusarium oxysporum f. vasinfectum 여러 가지 계통균이 혼재하는 토양에서는 밀, 벼 등 비기주작물도 이 균이 토양중에 생성하는 Fusaric acid에 의하여 종자발아나 발아후의 생육이 저해될 가능성이 있다.

• 식물조직배양학회지
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• 제26권3호
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• pp.205-209
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• 1999

지황 (Rehmannia glutinosa) 우량종묘 생산을 위하여 조직배양에 의해 생산된 종묘의 포장수량성을 평가하고 지표성분인 Catalpol과 유리당 함량 특성을 비교한 결과는 다음과 같다. 조직배양주의 종근저장성은 재래종에 비하여 약간 양호한 경향이었다. 또한 근장, 근경 등의 뿌리생육이 왕성하여 조직배양주의 수량성은 서천재래종 384kg/10 a에 비하여 1년차 묘는 112%, 2년차 묘는 246%까지 증수되는 결과를 보였다. 성분함량 특성비교에서는 총당함량이 서천재래 1.86%에 비하여 조직배양 1, 2년차 묘는 각각 1.21, 1.10%를 보였고, 지표성분인 Catalpol도 서천재래 0.46%에 비하여 조직배양 1, 2년차묘는 0.4%로 약간 감소되는 경향이었다. 이상의 결과에서 지황 조직배양주의 수량성은 재래종에 비하여 증수되는 특성을 보였다. 그러나 유리당과 Catalpol 함량은 약간 감소되는 경향이었는데 모본식물과 큰 차이를 보이지는 않았다.

• Journal of Plant Biology
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• 제30권1호
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• pp.69-77
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• 1987

From the single cell cultures of haploid tobacco (Nicotiana tabacum L. cv. NC 2326) glyphosate-resistant plants were regenerated. After treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and then inoculated onto the LS medium supplemented with 1 mM glyphosate, the single cells survived formed colonies and calluses in 65 and 95 days after culture, respectively, and then whole plants were regenerated in 0.1 mM glyphosate-containing medium from the selected calluses. There was no difference in fresh weight and shikimate content between the selected and normal haploid calluses. When sprayed with 0.1 mM glyphosate, the shikimate contents in the regenerated and normal plants were 0.659 and 20.816 mol/g fr. wt., while that in other normal plants which were not sprayed was 0.921. In addition, the calluses induced from the regenerated plants grew without showing any retardation when treated with glyphosate. These results indicate that the secelcted calluses and regenerated plants are resistant to glyphosate.

• Journal of Plant Biology
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• 제15권3호
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• pp.14-18
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• 1972

This paper includes a review on recent development on protoplast culture, regeneraton of plant from protoplast, and fusion of isolated protoplasts, and also describes the possibility of obtaining interspecific hybrid plants through asexual fusion of protoplasts of cells from distantly related plants which are not crossed by the ordinary sexual method.

• The Plant Pathology Journal
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• 제20권4호
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• pp.293-296
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• 2004

During the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) infection on tobacco plants, we found a bacterial isolate KTB3, and identified it as Acinetobacter sp. which strongly inhibited the infection of TMV When the culture filtrate from KTB3 was applied on the upper surface of the Xanthi-nc tobacco leaves at the same time, or 24 hours before TMV inoculation, almost complete inhibition was achieved. Likewise, 86% inhibition was achieved, when the culture filtrate was applied on the underside of the leaves. In field trials, transmission of TMV from diseased seedlings to healthy ones during transplanting work was reduced by 92%, when the culture filtrate was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. Antiviral substance from the culture filtrate was purified by ethanol precipitation, dialysis, DEAE-cellulose, and Sephadex G75 gel column chromatography. The partially purified active material which showed positive color reaction to sugar and protein inhibited TMV infection by 60% at 1 ${\mu}$g/ml.

• International Journal of Advanced Culture Technology
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• 제9권1호
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• pp.129-135
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• 2021

In this study, we built a mock-up of an indoor garden for private use and vertical gardens were installed on the walls of this indoor garden model. The purpose of this study is to examine the types of plants for best fit for growth and nurture in vertical garden and to identify the effects of indoor air quality improvement by these plants. As the result of the experiment, 22 species out of 32 species previously used for indoor garden was selected to be suitable for vertical gardens of a personal indoor garden. 10 species were found to be inappropriate for a personal indoor garden in terms of ornamental value, growth status and maintenance. The effect of plants on reducing CO2 has been proven by many studies. Also, through photosynthesis, plants combine CO2 with water and produce sugars and O2 (oxygen). Everyone accepts this fact. In nature, the production of oxygen is so important that without plants we would soon use it up and die. From the NASA Fact Sheet we know that air contains 20.95% O2 and 0.04% CO2. If you had enough plants in a room to use up all of the all of CO2 and convert it to oxygen, the oxygen levels would increase from 20.95% to 21%. This increase is difficult to detect and would have no effect on humans.

• 한국연초학회지
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• 제26권2호
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• pp.79-84
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• 2004

During the screening of antiviral substances having inhibitory effects on tobacco mosaic virus (TMV) infection to tobacco plants, we found a bacterial isolate KTGBP10, which was identified as a Stenotrophomonas sp., strongly inhibited the infection of TMV. When the culture filtrate from KTGBP10 was applied on the upper surface of leaves of Xanthi-nc tobacco plants at the same time or 24 hours before TMV inoculation, almost complete inhibition of TMV infection was achieved. And $40\%$ inhibition was shown with application of the culture filtrate to the under surface of leaves. In field trials, transmission of TMV from diseased seedlings to the healthy ones during transplanting work was reduced by $87.1\~92.6\%$ when the culture filtrate or cell suspension was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. When the broth filtrate of KTGBP10 was supplied by soaking through the cut-leaves before and/or after virus inoculation, the TMV infection was also inhibited by $50.4\~65.3\%$.

• 한국식생활문화학회지
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• 제1권2호
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• pp.167-170
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• 1986

The first historical record on the use of wild edible plants as foods in Korea involves sswuk and manul concerned with the mythology of Tangun. Numerous names of wild vegetables had been recorded in various ancient books. Wild edible plants are of great value as food resources and for domestication, since they have variable edible portions and quite a long picking season. Several kinds of wild edible plants have been already grown as vegetable crops. Doragi (Platycodon grandiflorum) is probably the one with the longest history of cultivation. During World War II, an attempt had been made to substitute vegetable crops for wild edible plants. As picking wild greens requires a great deal of labor and plants of wild growth are limited in the amount, domestication of wild vegetables as crops appears to be an urgent need for securing food resources in Korea.

• Plant Resources
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• 제6권2호
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• pp.153-158
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• 2003

Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.