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http://dx.doi.org/10.5423/PPJ.2009.25.4.369

Antiviral Effects of the Culture Filtrate from Serratia marcescens Gsm01, against Cucumber mosaic virus (CMV)  

Thapa, Shree Prasad (Division of Biological Environment, Kangwon National University)
Lee, Hye-Jin (Division of Bio-resources Technology, Kangwon National University)
Park, Duck-Hwan (Department of Forest Resources Protection, Kangwon National University)
Kim, Sam-Kyu (Division of Biological Environment, Kangwon National University)
Cho, Jun-Mo (Division of Biological Environment, Kangwon National University)
Cho, Sae-Youll (Division of Bio-resources Technology, Kangwon National University)
Hur, Jang-Hyun (Division of Biological Environment, Kangwon National University)
Lim, Chun-Keun (Division of Bio-resources Technology, Kangwon National University)
Publication Information
The Plant Pathology Journal / v.25, no.4, 2009 , pp. 369-375 More about this Journal
Abstract
The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMV-Y inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RT-PCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.
Keywords
culture filtrate; Cucumber mosaic virus; Serratia marcescens;
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1 Doubrava, N. S., Dean, R. A. and Kuc, J. 1988. Induction of systemic resistance to anthracnose caused by Colletotrichum lagenarium in cucumber by oxalate and extracts from spinach and rhubarb leaves. Physiol. Mol. Plant Pathol. 33:69-79   DOI
2 Green, P. J. 1994. The ribonucleases of higher plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45:421-445   DOI   ScienceOn
3 Kessman, H., Staub, T., Hofinann, C., Maetzke, T., Herzog, G., Ward, E., Uknes, S. and Ryals, J. 1994. Induction of systemic acquired disease resistance in plants by chemicals. Annu. Rev. Phytopathol. 32:439-460   DOI   ScienceOn
4 Maurhofer, M., Hase, C., Meuwly, P., Metraux, J. P. and Defago, G. 1994. Induction of systemic resistance of tobacco to Tobacco necrosis virus by the root-colonizing Pseudomonas fluorescens strain CHA0: Influence of the gacA gene and pyoverdine production. Phytopathology 84: 139-146   DOI   ScienceOn
5 Rivers, B. A, Bernatzky, R., Robinson, S. J. and Jahnen-Dechent, W. 1993. Molecular diversity at the self incompatibility locus is a salient feature in natural populations of wild tomato (Lyco-persiconpernvianum). Mol. Gen. Genet. 238:419-427   DOI   ScienceOn
6 Takanami, Y. 1981. A striking change in symptoms on cucumber mosaic virus-infected tobacco plants induced by a satellite RNA Virology 109:120-126   DOI   ScienceOn
7 Ehara, Y. and Mink, G. I. 1986. Inactivation of the yellow strain of cucumber mosaic virus by pancreatic ribonuclea'le. Ann. Phytopathol. Soc. Jpn. 52:260-269   DOI
8 Francki, R. I. B. 1968. Inactivation of cucumber mosaic virus (Q strain) nucleoprotein by pancreatic ribonuclease. Virology 34:694-700   DOI   PUBMED   ScienceOn
9 Ipper, N. S., Lee, S. H., Suk, J. K., Shrestha, A., Seo, D. U., Park, D. H., Cho, J. M., Park, D. S., Hur, J. H. and Lim, C. K. 2006. Isolation and evaluation of an antiviral producing Serratia spp. strain Gsm01 against Cucumber mosaic virus in Korea. Kor. J. Pest. Sci. 10:344-350
10 Palukaitis, P., Roossinck, M. J., Dietzgen R. G. and Frankl, R. B. 1992. Cucumber mosaic virus. Advances in Virus Research, pp.281-348. Academic Press, New York
11 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685   DOI   PUBMED   ScienceOn
12 Ryu, C.-M., Murphy, J. F., Mysore, K. S. and Kloepper, J. W. 2004. Plant growth promoting rhizobacteria systemically protect Arabidopsis thalina against Cucumber mosaic virus by a salicylic acid and NPR1-independent and jasmonic acid dependcnt signaling pathway. Plant J. 39:381-392   DOI   ScienceOn
13 Barbieri, L., Aron, G. M., Irvin, J. D. and Stirpe, F. 1982. Purification and partial characterization of another form of the antiviral protein from the seeds of Phytolacca americana L. (pokeweed). Biochem. J. 203:55-59   DOI   PUBMED
14 Liu, L., Kloepper, J. W. and Tuzun, S. 1995. Induction of systemic resistance in cucumber by plant growth-promoting rhizobacteria: Duration of protection and effect of host resistance on protection and root colonization. Phytopathology 85:1064-1068   DOI   ScienceOn
15 Venna, H., Varsha, N. and Baranwal, V. K. 1995. Endogenous Virus Inhibitors from Plants, Thcir Physical and Biological Properties. In: M. Chessin, D. Deborde and A Zipf (Eds.), Antiviral Proteins in Higher Plants, pp.1-21. CRC Press, Boca, Raton, FL
16 Cho, S. Y., Lee, S. H., Park, S., Choi, K. D., Cho, J. M., Hur, J. H., Shrestha, A. and Lim, C. K. 2009. Identification of a genetic locus related to antivirus production in pseudomonas fluorescence strain GpfOl against Cucumber mosaic virus. Plant Pathol. J. 25:77-85   DOI   ScienceOn
17 Fitchen, J. and Beachy R. N. 1993. Genetically engineered protection against viruses in transgenic plants. Annu. Rev. Microbiol. 47:739-763   DOI   ScienceOn
18 Murphy, J. F., Reddy, M. S. C., Ryu, M., Kloepper, J. W. and Li, R. 2003. Rhizobacteria-mediated growth promotion of tomato leads to protection against Cucumber mosaic virus. Phytopathology 93:1301-1307   DOI   ScienceOn
19 Zitter, T A., 1991. Discases caused by viruses. In: Jones, J. B., Jones, J. P., Stall, R. E. and Zitter, T. A (eds), Compendium of Tomato Diseases. The American Phytopathological Society, pp. 3142. St. Paul, MN., USA
20 Someya, N., Kataoka, N., Komagata, T, Hirayae, K., Hibi, T. and Akutsu, K. 2000. Biological control of cyclamen soil borne diseases by Serratia marcescens strain B2. Plant Dis. 84:334-340   DOI   ScienceOn
21 Kubo, S. Ikeda, T, Imaizumi, S., Takanami, Y. and Mikami, Y. 1990. A potent plant virus inhibitor found in Mirabilis jalapa L. Ann. Phytopath. Soc. Japan 56:481-487   DOI
22 Raupach, G. S., Liu, L., Murphy, J., Tuzun, S. and Kloepper, J. W. 1996. Induced systemic resistance in cucumber and tomato against cucumber mosaic cucumovirus using plant growth-promoting rhizobacteria (PGPR). Plant Dis. 80:891-894   DOI   ScienceOn
23 Clark, M. F. and Adams, A. N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483   DOI   ScienceOn
24 Ghandi, H. A. 2000. Bcnzo-(1, 2, 3)-thiodiazole-7 -carbothioic acid S-methyl ester induces systemic resistance in tomato (Lycopersicon esculentum. Mill cv. Vollendung) to Cucumber mosaic virus. Crop Protect. 19:401-405   DOI   ScienceOn
25 Moiseyev, G. P., Fedoreyeva, L. I., Zhuravlev, Y. N., Yanetskaya, E., Jekel, P. A. and Beintema, J. J. 1997. Primary structures of two ribonucleases from ginseng calluses. New member of the PR-10 family of intracellular pathogenesis-related protein. FEBS Lett. 402:207-210
26 Bergstorm, G. C., Johnson, M. C. and Kuc, J. 1982. Effect of local infection of cucumber by Colletotrichum lagenarium, Pseudomonas lachrymans, or tobacco necrosis virus on systemic resistance to cucumber mosaic virus. Phytopathology 72:922-926   DOI